scholarly journals Restriction-Site Heteroplasmy in Anchovy (Engraulis encrasicolus) Indicates Incidental Biparental Inheritance of Mitochondrial DNA

1993 ◽  
Keyword(s):  
Mitochondrial Dna ◽  
Restriction Site ◽  
Biparental Inheritance ◽  
Engraulis Encrasicolus

Assignment of two mitochondrially synthesized polypeptides to human mitochondrial DNA and their use in the study of intracellular mitochondrial interaction

1982 ◽  
Vol 2 (1) ◽  
pp. 30-41
Author(s):  
N A Oliver ◽  
D C Wallace
Keyword(s):  
Mitochondrial Dna ◽  
Restriction Site ◽  
Mitochondrial Protein ◽  
Mitochondrial Protein Synthesis ◽  
Human Mitochondrial Dna ◽  
Ht1080 Cells ◽  
Mitochondrial Dnas ◽  
A Cell ◽  
Dna Restriction ◽  
Restriction Site Polymorphisms

Two mitochondrially synthesized marker polypeptides, MV-1 and MV-2, were found in human HeLa and HT1080 cells. These were assigned to the mitochondrial DNA in HeLa-HT1080 cybrids and hybrids by demonstrating their linkage to cytoplasmic genetic markers. These markers include mitochondrial DNA restriction site polymorphisms and resistance to chloramphenicol, an inhibitor of mitochondrial protein synthesis. In the absence of chloramphenicol, the expression of MV-1 and MV-2 in cybrids and hybrids was found to be directly proportional to the ratio of the parental mitochondrial DNAs. In the presence of chloramphenicol, the marker polypeptide linked to the chloramphenicol-sensitive mitochondrial DNA continued to be expressed. This demonstrated that resistant and sensitive mitochondrial DNAs can cooperate within a cell for gene expression and that the CAP-resistant allele was dominant or codominant to sensitive. Such cooperation suggests that mitochondrial DNAs can be exchanged between mitochondria.

Fate of genetically marked mitochondrial DNA from spermatocytes microinjected into mouse zygotes

Zygote ◽  
1999 ◽  
Vol 7 (2) ◽  
pp. 151-156 ◽  
Author(s):  
James M. Cummins ◽  
Hidefumi Kishikawa ◽  
Denise Mehmet ◽  
Ryuzo Yanagimachi
Keyword(s):  
Mitochondrial Dna ◽  
Germ Cells ◽  
Restriction Site ◽  
Mutual Recognition ◽  
Nuclear Genome ◽  
Pcr Amplification ◽  
Cell Stage ◽  
Mt Dna ◽  
Mouse Zygotes ◽  
Host Embryo

Cytoplasts from single spermatocytes of NZB/BinJ mice were separated from the nuclei and individually microinjected into B6D2F1 (C57BL/6 × DNBA/2J) hybrid embryos at the pronuclear stage (20 h after hCG injection). Of 363 zygotes injected, 311 (86%) survived and developed. From these experiments, we transferred 222 embryos into 20 pseudopregnant recipients. Eighteen (90%) became pregnant and 82 pups were born (37% of transfers). Mitochondrial DNA (mt DNA) from the NZB/BinJ strain lacks a RsaI restriction site and can thus be distinguished from the host embryo following PCR amplification. We were unable to detect the transferred mtDNA in blastocysts on day 4–5 after injection. Nor could we detect NZB/BinJ mtDNA in placentae, nor in tissues from mice born to host mothers following the transfer of blastocysts that developed from injected zygotes. Rejection of paternal mitochondria by the embryo normally occurs at the 4- to 8-cell stage in mice and is apparently dependent on mutual recognition between the mitochondria and the nuclear genome. We conclude that this mechanism has probably already developed by the time the germ cells have become committed to meiosis.

The Drosophila montium subgroup species. Phylogenetic relationships based on mitochondrial DNA analysis

Genome ◽  
1996 ◽  
Vol 39 (5) ◽  
pp. 874-883 ◽  
Author(s):  
Nikolas Nikolaidis ◽  
Zacharias G. Scouras
Keyword(s):  
Mitochondrial Dna ◽  
Phylogenetic Relationships ◽  
Phylogenetic Trees ◽  
Dna Analysis ◽  
Restriction Site ◽  
Species Group ◽  
The Other ◽  
Complex Species ◽  
Restriction Site Maps ◽  
Montium Subgroup

Mitochondrial DNA (mtDNA) restriction site maps for three Drosophila montium subgroup species of the melanogaster species group, inhabiting Indian and Afrotropical montium subgroup territories, were established. Taking into account previous mtDNA data concerning six oriental montium species, a phylogeny was established using distance-matrix and parsimony methods. Both genetic diversity and mtDNA size variations were found to be very narrow, suggesting close phylogenetic relationships among all montium species studied. The phylogenetic trees that were constructed revealed three main lineages for the montium subgroup species studied: one consisting of the Afrotropical species Drosophila seguyi, which is placed distantly from the other species, one comprising the north-oriental (Palearctic) species, and one comprising the southwestern (south-oriental, Australasian, Indian, and Afrotropical) species. The combination of the mtDNA data presented here with data from other species belonging to the melanogaster and obscura subgroups revealed two major clusters: melanogaster and obscura. The melanogaster cluster is further divided into two compact lineages, comprising the montium subgroup species and the melanogaster complex species; the species of the other complex of the melanogaster subgroup, yakuba, disperse among the obscura species. The above grouping is in agreement with the mtDNA size variations of the species. Overall, among all subgroups studied, the species of the montium subgroup seem to be the most closely related. Key words : mtDNA restriction site maps, mtDNA size variations, Drosophila, phylogeny.

Mitochondrial DNA restriction site variation in Tasmanian populations of Orange Roughy (Hoplostethus atlanticus), a deep-water marine teleost

1989 ◽  
Vol 40 (1) ◽  
pp. 1 ◽  
Author(s):  
JR Ovenden ◽  
AJ Smolenski ◽  
RWG White
Keyword(s):  
Mitochondrial Dna ◽  
West Coast ◽  
East Coast ◽  
Restriction Site ◽  
The West ◽  
Orange Roughy ◽  
Site Change ◽  
Restriction Site Variation ◽  
Site Variation ◽  
Hoplostethus Atlanticus

The orange roughy, Hoplostethus atlanticus, forms the basis of an important trawl fishery in Australia and New Zealand which is currently being over-exploited. To gain information about the stock structure of an Australian orange roughy population, ten restriction enzymes were used to survey the restriction- site variation in the mitochondrial genome of 23 individuals collected from the east coast and 26 individuals collected from the west coast of Tasmania. The mean diversity between all 49 genomes was a low 0.19%. Of the eleven haplotypes identified, only one was present in both the east and west coast samples. Four haplotypes were found only in the east coast sample and six were found only in the west coast sample. Eight haplotypes were related to the haplotype found on both coasts by the gain or loss of one restriction site. One haplotype collected in the east coast sample was related to one other east coast haplotype by one site change. One haplotype collected from the west coast was related to a west coast haplotype by one site change. If this pattern is confirmed by further mitochondrial DNA studies, it could be inferred that gene flow between the orange roughy populations on the east and west coasts of Tasmania is low.

Biparental inheritance of organelles in Pelargonium: evidence for intergenomic recombination of mitochondrial DNA

Planta ◽  
2012 ◽  
Vol 237 (2) ◽  
pp. 509-515 ◽  
Author(s):  
Janina Apitz ◽  
Andreas Weihe ◽  
Frank Pohlheim ◽  
Thomas Börner
Keyword(s):  
Mitochondrial Dna ◽  
Biparental Inheritance ◽  
Intergenomic Recombination

scholarly journals Heteroplasmy variability in individuals with biparentally inherited mitochondrial DNA

2020 ◽  
Author(s):  
Jesse Slone ◽  
Weiwei Zou ◽  
Shiyu Luo ◽  
Eric S Schmitt ◽  
Stella Maris Chen ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Single Cell ◽  
Strong Evidence ◽  
Genetic Factors ◽  
Single Cell Level ◽  
Biparental Inheritance ◽  
Cell Level ◽  
Single Population ◽  
Parent Of Origin ◽  
Two Populations

ABSTRACTWith very few exceptions, mitochondrial DNA (mtDNA) in humans is transmitted exclusively from mothers to their offspring, suggesting the presence of a strong evolutionary pressure favoring the exclusion of paternal mtDNA. We have recently shown strong evidence of paternal mtDNA transmission. In these rare situations, males exhibiting biparental mtDNA appear to be limited to transmitting just one of the mtDNA species to their offspring, while females possessing biparental mtDNA populations consistently transmit both populations to their offspring at a very similar heteroplasmy level. The precise biological and genetic factors underlying this unusual transmission event remain unclear. Here, we have examined heteroplasmy levels in various tissues among individuals with biparental inheritance. Our results indicate that individuals with biparental mtDNA have remarkable inter-tissue variability in heteroplasmy level. At the single-cell level, paternal mtDNA heteroplasmy in sperm varies dramatically, and many sperm possess only one of the two mtDNA populations originally in question. These results show a fundamental, parent-of-origin difference in how mtDNA molecules transmit and propagate. This helps explain how a single population of mtDNAs are transmitted from a father possessing two populations of mtDNA molecules, suggesting that some mtDNA populations may be favored over others when transmitted from the father.

Sign in / Sign up

Export Citation Format

Share Document