With the development of fluorescent technology, the application area of fluorescent material is expanding, which includes the fields of traffic, national defence, architecture, detection, decoration etc. In the field of printing, whether in the value of utility or view and admire, fluorescent adds tremendous energies. However, a majority of fluorescent materials used nowadays contain radioelement, which will do harm to human health. This paper considers the needs of fluorescent map printing, according to the defects of traditional fluorescent material such as low brightness, short duration time, strong radioactivity, limitation to spot color printing etc. It improves the capability of long fluorescent lag material and makes red, blue, green and yellow long fluorescent lag materials. Meanwhile, it tests the influence of ink thickness, illumination time and granule size etc. on irradiance capability. The thicker the ink is, the longer of the lag time will be. The bigger the granule size is, the lag time will be. It also tests its radioactivity extent, which is in the safety range and reaches the standard of environmental protection. According to the special requirement of map printing, this paper brings forward the technique scheme of the application of long fluorescent lag ink in map printing,which realizes the color fluorescent map printing.