scholarly journals Relationship between inflammatory biomarkers and oxidative stress with uterine health in dairy cows with different dry period lengths1

2019 ◽  
Vol 3 (2) ◽  
pp. 607-619
Author(s):  
Novi Mayasari ◽  
Erminio Trevisi ◽  
Annarita Ferrari ◽  
Bas Kemp ◽  
Henk K Parmentier ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dairy Cows ◽  
Negative Energy ◽  
Inflammatory Biomarkers ◽  
Early Lactation ◽  
Dry Period ◽  
Inflammatory Status ◽  
Uterine Health ◽  
Uterine Environment ◽  
And Oxidative Stress

Abstract Earlier studies indicated that the inflammatory status of dairy cows in early lactation could not be fully explained by the negative energy balance (NEB) at that moment. The objective of the present study was to determine relationships between inflammatory biomarkers and oxidative stress with uterine health in dairy cows after different dry period lengths. Holstein–Friesian dairy cows were assigned to one of three dry period lengths (0-, 30-, or 60-d) and one of two early lactation rations (glucogenic or lipogenic ration). Cows were fed either a glucogenic or lipogenic ration from 10-d before the expected calving date. Part of the cows which were planned for a 0-d dry period dried themselves off and were attributed to a new group (0 → 30-d dry period), which resulted in total in four dry period groups. Blood was collected (N = 110 cows) in weeks −3, −2, −1, 1, 2, and 4 relative to calving to determine biomarkers for inflammation, liver function, and oxidative stress. Uterine health status (UHS) was monitored by scoring vaginal discharge (VD) based on a 4-point scoring system (0, 1, 2, or 3) in weeks 2 and 3 after calving. Cows were classified as having a healthy uterine environment (HU, VD score = 0 or 1 in both weeks 2 and 3), nonrecovering uterine environment (NRU, VD score = 2 or 3 in week 3), or a recovering uterine environment (RU, VD score = 2 or 3 in week 2 and VD score= 0 or 1 in week 3). Independent of dry period length, cows with NRU had higher plasma haptoglobin (P = 0.05) and lower paraoxonase levels (P < 0.01) in the first 4 weeks after calving and lower liver functionality index (P < 0.01) compared with cows with HU. Cows with NRU had lower plasma albumin (P = 0.02) and creatinine (P = 0.02) compared with cows with a RU, but not compared with cows with HU. Independent of UHS, cows with a 0 → 30-d dry period had higher bilirubin levels compared with cows with 0-, 30-, or 60-d dry period (P < 0.01). Cows with RU and fed a lipogenic ration had higher levels of albumin in plasma compared with cows with NRU and fed a lipogenic ration (P < 0.01). In conclusion, uterine health was related to biomarkers for inflammation (haptoglobin and albumin) and paraoxonase in dairy cows in early lactation. Cows which were planned for a 0-d dry period, but dried themselves off (0 → 30-d dry period group) had higher bilirubin levels, which was possibly related to a more severe NEB in these cows. Inflammatory biomarkers in dairy cows in early lactation were related to uterine health in this period.

scholarly journals Effects of dry period length and dietary energy source on inflammatory biomarkers and oxidative stress in dairy cows

2017 ◽  
Vol 100 (6) ◽  
pp. 4961-4975 ◽  
Author(s):  
N. Mayasari ◽  
J. Chen ◽  
A. Ferrari ◽  
R.M. Bruckmaier ◽  
B. Kemp ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Energy Source ◽  
Dairy Cows ◽  
Period Length ◽  
Inflammatory Biomarkers ◽  
Dietary Energy ◽  
Dry Period ◽  
And Oxidative Stress

scholarly journals Integrated Metabolomics and Proteomics Dynamics of Serum Samples Reveals Dietary Zeolite Clinoptilolite Supplementation Restores Energy Balance in High Yielding Dairy Cows

Metabolites ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 842
Author(s):  
Sudipa Maity ◽  
Ivana Rubić ◽  
Josipa Kuleš ◽  
Anita Horvatić ◽  
Dražen Đuričić ◽  
...  
Keyword(s):  
Energy Balance ◽  
Dairy Cows ◽  
Interaction Analysis ◽  
Negative Energy ◽  
Exchange Property ◽  
Early Lactation ◽  
Differentially Expressed Proteins ◽  
Limited Information ◽  
Dry Period ◽  
Serum Samples

Dairy cows can suffer from a negative energy balance (NEB) during their transition from the dry period to early lactation, which can increase the risk of postpartum diseases such as clinical ketosis, mastitis, and fatty liver. Zeolite clinoptilolite (CPL), due to its ion-exchange property, has often been used to treat NEB in animals. However, limited information is available on the dynamics of global metabolomics and proteomic profiles in serum that could provide a better understanding of the associated altered biological pathways in response to CPL. Thus, in the present study, a total 64 serum samples were collected from 8 control and 8 CPL-treated cows at different time points in the prepartum and postpartum stages. Labelled proteomics and untargeted metabolomics resulted in identification of 64 and 21 differentially expressed proteins and metabolites, respectively, which appear to play key roles in restoring energy balance (EB) after CPL supplementation. Joint pathway and interaction analysis revealed cross-talks among valproic acid, leucic acid, glycerol, fibronectin, and kinninogen-1, which could be responsible for restoring NEB. By using a global proteomics and metabolomics strategy, the present study concluded that CPL supplementation could lower NEB in just a few weeks, and explained the possible underlying pathways employed by CPL.

scholarly journals Effect of parity on metabolic and oxidative stress profiles in Holstein dairy cows

2020 ◽  
Vol 13 (12) ◽  
pp. 2780-2786
Author(s):  
Shimaa G. Yehia ◽  
Eman S. Ramadan ◽  
Eissa A. Megahed ◽  
Noha Y. Salem
Keyword(s):  
Oxidative Stress ◽  
Health Status ◽  
Dairy Cows ◽  
Total Protein ◽  
Negative Energy ◽  
Total Antioxidant ◽  
Pregnancy And Lactation ◽  
Stress Profiles ◽  
Stress Patterns ◽  
And Oxidative Stress

Background and Aim: Pregnancy and lactation have an impact on health status of animals and constitute burden on body metabolites and the oxidant-antioxidant equilibrium. This study is aimed at evaluating metabolic and oxidative stress patterns and parity impacts in both primiparous and multiparous dairy cows. Materials and Methods: Twenty-seven primiparous and multiparous Holstein cows were enrolled and categorized into four groups according to their physiologic status: Primiparous peripartum heifer (n=5), primiparous postpartum cow (n=9), multiparous peripartum cows (n=5), and multiparous postpartum cows (n=8). Blood sample was taken from each animal – peripartum groups at 3 weeks prepartum and postpartum groups at 3 weeks post-parturition – for complete blood picture, glucose, cholesterol, triglyceride, total protein, albumin, non-esterified fatty acids (NEFA), malondialdehyde (MDA), total antioxidant capacity, and haptoglobin estimation. Results: Postpartum primiparous cows showed significant decrease in glucose, total protein, and albumin while showing significant increase in MDA, NEFA, and globulin; on the other hand, multiparous postpartum cows showed significant decrease in glucose, total protein, and albumin, associated with significant increase in cholesterol and MDA when compared with prepartum PP and MP cows, respectively. Postpartum multiparous cows significantly showed reduction in NEFA when compared to primiparous postpartum cows. Hematologic profiles of postpartum primiparous and multiparous cows showed significant decrease in red blood cells and packed cell volume, significant increase in lymphocytes when compared with prepartum cows. Conclusion: Metabolic and oxidative abnormalities exist in both primiparous and multiparous cows during the transition phase, however postpartum primiparous cows show higher susceptibility to negative energy balance impacts. Oxidant/ antioxidant imbalance occurred in both the primiparous and multiparous postpartum cows, highlighting the importance of oxidative stress profiles in the assessment of metabolic health status during transition.

scholarly journals Changes in biomarkers of nutrient metabolism, inflammation, and oxidative stress in dairy cows during the transition into the early dry period

2018 ◽  
Vol 101 (10) ◽  
pp. 9350-9359 ◽  
Author(s):  
A.K. Putman ◽  
J.L. Brown ◽  
J.C. Gandy ◽  
L. Wisnieski ◽  
L.M. Sordillo
Keyword(s):  
Oxidative Stress ◽  
Dairy Cows ◽  
Dry Period ◽  
Nutrient Metabolism ◽  
And Oxidative Stress

Effect of dietary energy content pre- and post-calving on production and blood metabolites of dairy cows during early lactation

2009 ◽  
Vol 2009 ◽  
pp. 11-11
Author(s):  
R A Law ◽  
F J Young ◽  
D C Patterson ◽  
D J Kilpatrick ◽  
A R G Wylie ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Energy Content ◽  
Post Partum ◽  
Energy Levels ◽  
Negative Energy ◽  
Dietary Energy ◽  
Early Lactation ◽  
Dry Period ◽  
Energy Demands ◽  
Increased Risk

The increase in milk production potential of the modern high yielding dairy cow has resulted in excessive and prolonged negative energy balance (NEB) during early lactation. This predisposes the cow to an increased risk of metabolic disorders, poor fertility and subsequently increased culling rates. Historically, much emphasis has been placed on post partum nutrition in an attempt to suppress these ill effects. However, contemporary theories advocate improved dry cow nutrition to properly prepare the cow for energy demands of early lactation. Friggens et al. (2004) stated that priming the liver during the dry period would allow the cow to better deal with metabolic processes in the post partum period. In this experiment, dairy cows were offered different dietary energy levels pre-and post-calving in an attempt to quantify the effects on energy parameters during the same time periods.

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