Maternal and congenital infections arising from Zika, dengue and Chikungunya arboviruses in Salvador, Brazil

2019 ◽  
Vol 114 (3) ◽  
pp. 222-225
Author(s):  
Alan Oliveira Duarte ◽  
João Vitor Oliveira ◽  
Tereza Cristina Xavier Carvalho ◽  
Lorena de Brito Pessoa ◽  
Cláudio Magalhães Filho ◽  
...  
Keyword(s):  
Zika Virus ◽  
Neonatal Screening ◽  
Congenital Infection ◽  
Cross Sectional Study ◽  
Igg Antibodies ◽  
Sectional Study ◽  
Cross Sectional ◽  
High Exposure ◽  
Congenital Infections ◽  
Igm Antibodies

Abstract Background Salvador was one of the Brazilian cities most affected during the 2015 Zika virus (ZIKV) outbreak. Methods A cross-sectional study was performed with enrolment of parturients and their newborns. Results Positive IgM antibodies for ZIKV, dengue (DENV) and Chikungunya (CHIKV) were present in 6.9, 11.9 and 22.8% of the parturients, and IgG antibodies were detected in 72.3, 92.3 and 38.6%, respectively. No cases of DENV congenital infection were identified. ZIKV and CHIKV congenital infections were observed in 16.5 and 13% of newborns, respectively. Conclusions High exposure rates to the three arboviruses and the identification of newborns with ZIKV and CHIKV congenital infections reinforces the necessity of ZIKV and CHIKV prenatal and neonatal screening in endemic regions.

scholarly journals Pediatric neurodevelopment by prenatal Zika virus exposure: A cross-sectional study of the Microcephaly Epidemic Research Group Cohort

2020 ◽  
Author(s):  
paula sobral Silva ◽  
Sophie Helena Eickmann ◽  
Ricardo Arraes de Alencar Ximenes ◽  
Ulisses Ramos Montarroyos ◽  
Marília de Carvalho Lima ◽  
...  
Keyword(s):  
At Risk ◽  
Zika Virus ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Early Assessment ◽  
Cross Sectional ◽  
Group 4 ◽  
Neurological Abnormalities ◽  
Development Delay ◽  
And Behavior

Abstract Background: The implications of congenital Zika Virus (ZIKV) infections for pediatric neurodevelopment and behavior remain inadequately studied. The aim of this study is to investigate patterns of neurodevelopment and behavior in children with different levels of ZIKV-related microcephaly or with prenatal ZIKV exposure in the absence of microcephaly. Methods: We conducted a cross-sectional study, nested in a cohort, of 274 children (aged 10-45 months) who were born during the peak and decline of the microcephaly epidemic in Northeast Brazil. Participants were evaluated between February 2017 and August 2019 at two tertiary care hospitals in Recife, Brazil. We analyzed the children in four groups assigned based on clinical and laboratory criteria: Group 1 had severe microcephaly; Group 2 had moderate microcephaly; Group 3 had prenatal ZIKVxposure confirmed by maternal RT-PCR testing and no microcephaly; and Group 4 was a neurotypical control group. Groups were evaluated clinically for neurological abnormalities and compared using the Survey of Wellbeing of Young Children (SWYC), a neurodevelopment and behavior screening instrument, and a SWYC adapted form to compare severe cases. Results: Based on the SWYC screening, we observed differences between the groups for developmental milestones but not behavior. Among children with severe microcephaly of whom 98.2% presented with neurological abnormalities, 99.1% were at risk of development delay, and presented similar performance whether evaluated under or over 24 months of age. Among children with moderate microcephaly of whom 60% presented with neurological abnormalities, 65% were at risk of development delay. For children without microcephaly, the percentages found to be at risk of developmental delays were markedly lower and did not differ by prenatal ZIKV exposure status: Groups 3, 13.8%; Group 4, 21.7%. Conclusions: Among groups of children with prenatal ZIKV exposure, we found a gradient of risk of development delay. Children with severe microcephaly were at highest risk, while normocephalic ZIKV-exposed children had similar risks to unexposed control children. We propose that ZIKV-exposed children should undergo first-line screening for neurodevelopment and behavior using the SWYC. Early assessment and follow-up will enable at-risk children to be referred to a more comprehensive developmental evaluation and to multidisciplinary care management.

Relation between IgG antibodies to foods and IgE antibodies to milk, egg, cat, dog and/or mite in a cross-sectional study

1999 ◽  
Vol 29 (5) ◽  
pp. 604-610 ◽  
Author(s):  
EYSINK ◽  
DE JONG ◽  
BINDELS ◽  
SCHARP-VAN DER LINDEN ◽  
DE GROOT ◽  
...  
Keyword(s):  
Cross Sectional Study ◽  
Igg Antibodies ◽  
Sectional Study ◽  
Cross Sectional ◽  
Ige Antibodies

scholarly journals Hemoglobinopathies in newborns in the southern region of the Triângulo Mineiro, Brazil. Cross-sectional study

2015 ◽  
Vol 133 (5) ◽  
pp. 439-444 ◽  
Author(s):  
Aline Menezes Carlos ◽  
Renata Andréia Volpe Souza ◽  
Bruna Maria Bereta de Souza ◽  
Gilberto de Araujo Pereira ◽  
Sebastião Tostes Júnior ◽  
...  
Keyword(s):  
Neonatal Screening ◽  
Screening Program ◽  
Cross Sectional Study ◽  
Alkaline Ph ◽  
Sectional Study ◽  
Cross Sectional ◽  
Hb E ◽  
Hb S ◽  
Neonatal Screening Program ◽  
Hb C

ABSTRACT CONTEXT AND OBJECTIVE: Hemoglobinopathies are among the commonest and most widespread genetic disorders worldwide. Their prevalence varies according to ethnic composition and/or geographical region. The aim of this study was to investigate the presence of hemoglobinopathies and their association with ethnicity among 1,004 newborns, to confirm the guideline of the Brazilian National Neonatal Screening Program. DESIGN AND SETTING: Cross-sectional study conducted in a public referral hospital in the Triângulo Mineiro region, Minas Gerais, Brazil. METHODS: Qualitative assessment of hemoglobin was performed through electrophoresis on cellulose acetate: at alkaline pH to identify the hemoglobin (Hb) profile and at acid pH to differentiate Hb S from Hb D and Hb C from Hb E and others that migrate to similar positions at alkaline pH. Neutral pH was used to detect Hb Bart's identified in alpha thalassemia (α-thal). The elution method after electrophoresis was used to quantitatively assess hemoglobins. RESULTS: There was predominance of α-thal, with 105 cases (10.46%), followed by Hb S with 61 cases (6.08%, comprising 46 Hb AS, 2 Hb SS and 13 Hb S/α-thal), 9 cases (0.9%) of Hb AC and 6 cases (0.6%) suggestive of beta thalassemia (β-thal). The frequency of hemoglobinopathies was significantly higher among Afro-descendants. CONCLUSIONS: These findings corroborated of the National Neonatal Screening Program for diagnosing sickle cell disease and Hb C, Hb D, Hb E and β-thal hemoglobinopathies.

Cross-sectional study of bone metabolism with nutrition in adult classical phenylketonuric patients diagnosed by neonatal screening

2011 ◽  
Vol 29 (6) ◽  
pp. 737-743 ◽  
Author(s):  
Hironori Nagasaka ◽  
Hirokazu Tsukahara ◽  
Tomozumi Takatani ◽  
Yoshitami Sanayama ◽  
Masaki Takayanagi ◽  
...  
Keyword(s):  
Bone Metabolism ◽  
Neonatal Screening ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional

scholarly journals Dengue in the campus community of an overseas American university: A cross-sectional study

2019 ◽  
Vol 13 (03) ◽  
pp. 233-239
Author(s):  
Hamish Mohammed ◽  
Mary H Hayden ◽  
Elise Lee ◽  
Luis M Santiago ◽  
Rosina C Krecek ◽  
...  
Keyword(s):  
Risk Factors ◽  
Logistic Regression ◽  
Denv Infection ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Campus Community ◽  
Igg Antibodies ◽  
Sectional Study ◽  
Cross Sectional ◽  
American University

Introduction: After a large outbreak of dengue virus (DENV) serotype-3 in Saint Kitts and Nevis (SKN) in 2008, we performed a cross-sectional study to determine the prevalence of anti-DENV immunoglobulin G (IgG) antibodies in expatriate and local persons affiliated with an American veterinary school there. Methodology: This campus community comprised mostly expatriate students and faculty and Kittitian administrative staff. In 2009, a stratified random sample of students, faculty and staff was invited to complete an electronic survey to assess risk factors for DENV and provide blood for testing for anti-DENV IgG antibodies by an enzyme-linked immunosorbent assay. IgG-positive specimens were also tested by a 90% plaque reduction neutralization test (PRNT90) to determine immunoreactivity to DENV (1-4) serotypes and West Nile virus. Risk factors for anti-DENV IgG seropositivity were determined using simple and adjusted logistic regression. Results: Of the 118 participants, the overall prevalence of DENV IgG antibodies was 44.1% (95% confidence interval [CI]: 35.1-53.0%), ranging from 30.1% in students, 100.0% in staff and 57.9% in faculty (p < 0.001). Duration of residence in St. Kitts was the only variable significantly associated with seropositivity on multiple logistic regression (adjusted odds ratio [95% CI]: 1.21 [1.07-1.37]). The serotype of DENV was determined in 11 persons: DENV-1 (n = 4), DENV-2 (n = 3), and DENV-3 (n = 4). Conclusions: Expatriate students and faculty moving to St. Kitts from non-endemic areas were at high risk of DENV infection. There is a need for increased emphasis on pre-travel mosquito-borne virus prevention education for persons moving to St. Kitts to study and work.

scholarly journals Evaluation of nine serological rapid tests for the detection of SARS-CoV-2

2020 ◽  
Vol 44 ◽  
pp. 1
Author(s):  
Marcela Mercado ◽  
Jeadran Malagón-Rojas ◽  
Gabriela Delgado ◽  
Vivian Vanesa Rubio ◽  
Lida Muñoz Galindo ◽  
...  
Keyword(s):  
Study Groups ◽  
Cross Sectional Study ◽  
Igg Antibodies ◽  
Sectional Study ◽  
Rt Pcr ◽  
Serum Samples ◽  
Serological Tests ◽  
Cross Sectional ◽  
Diagnostic Aid ◽  
Rapid Tests

Objective. To evaluate the operative capacity of nine serological rapid tests to detect the IgM/IgG antibodies response in serum from patients with SARS-CoV-2 in different clinical stages. Methods. A cross-sectional study of serological rapid tests was designed to compare the performance of the evaluated immunochromatographic tests for the diagnosis of SARS-CoV-2. A total of 293 samples was used, including negatives, asymptomatic, and symptomatic serum samples. Results. The sensitivity of the evaluated tests was low and moderate in the groups of asymptomatic serum samples and the group of serums coming from patients with less than 11 days since the onset of the symptoms. The specificity for the anti-SARS-CoV-2 antibodies tests ranged between 86.5%-99% for IgM and 86.5%-99.5% for IgG. The sensitivity and the likelihood ratio were different according to the study groups. The usefulness of these tests is restricted to symptomatic patients and their sensitivity is greater than 85% after 11 days from the appearance of symptoms. Conclusions. Serological tests are not an adequate strategy for the identification of asymptomatic and pre-symptomatic patients. Serological rapid tests for the detection of specific anti-SARS-CoV-2 antibodies can be used as a diagnostic aid, but diagnosis must be confirmed by RT-PCR. Rapid tests should be reserved for patients with symptoms lasting more than 11 days.

scholarly journals Pediatric neurodevelopment by prenatal Zika virus exposure: A cross-sectional study of the Microcephaly Epidemic Research Group Cohort

2020 ◽  
Author(s):  
Paula Fabiana Sobral Silva ◽  
Sophie Helena Eickmann ◽  
Ricardo Arraes de Alencar Ximenes ◽  
Ulisses Ramos Montarroyos ◽  
Marília de Carvalho Lima ◽  
...  
Keyword(s):  
At Risk ◽  
Zika Virus ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Group 4 ◽  
Neurological Abnormalities ◽  
Development Delay ◽  
Group 3 ◽  
And Behavior

Abstract Background: The implications of congenital Zika Virus (ZIKV) infections for pediatric neurodevelopment and behavior remain inadequately studied. The aim of this study is to investigate patterns of neurodevelopment and behavior in groups of children with differening severities of ZIKV-related microcephaly and children with prenatal ZIKV exposure in the absence of microcephaly.Methods: We conducted a cross-sectional study, nested in a cohort, of 274 children (aged 10-45 months) who were born during the peak and decline of the microcephaly epidemic in Northeast Brazil. Participants were evaluated between February 2017 and August 2019 at two tertiary care hospitals in Recife, Pernambuco, Brazil. We analyzed the children in four groups assigned based on clinical and laboratory criteria: Group 1 had severe microcephaly; Group 2 had moderate microcephaly; Group 3 had prenatal ZIKVexposure confirmed by maternal RT-PCR testing but no microcephaly; and Group 4 was a neurotypical control group. Groups were evaluated clinically for neurological abnormalities and compared using the Survey of Wellbeing of Young Children (SWYC), a neurodevelopment and behavior screening instrument validated for use in Brazil. Children with severe delays underwent further evaluation with an adapted version of the SWYC.Results: Based on the SWYC screening, we observed differences between the groups for developmental milestones but not behavior. Among the 114 children with severe microcephaly of whom 98.2% presented with neurological abnormalities, 99.1% were ‘at risk of development delay’ according to the SWYC instrument. Among the 20 children with moderate microcephaly of whom 60% presented with neurological abnormalities, 65% were ‘at risk of development delay’. For children without microcephaly, the percentages found to be ‘at risk of developmental delay’ were markedly lower and did not differ by prenatal ZIKV exposure status: Group 3 (N=94), 13.8%; Group 4 (N=46), 21.7%.Conclusions: Among children with prenatal ZIKV exposure, we found a gradient of risk of development delay according to head circumference. Children with severe microcephaly were at highest risk for delays, while normocephalic ZIKV-exposed children had similar risks to unexposed control children. We propose that ZIKV-exposed children should undergo first-line screening for neurodevelopment and behavior using the SWYC instrument. Early assessment and follow-up will enable at-risk children to be referred to a more comprehensive developmental evaluation and to multidisciplinary care management.

Prevalence and risk factors of Hantavirus infection in patients undergoing hemodialysis in Khartoum, Sudan, in 2019: a cross-sectional study

2020 ◽  
Author(s):  
Azza A Abbas ◽  
Hiba A A Ali ◽  
Mohamed A Alagib ◽  
Huda F S Salih ◽  
Isam M Elkhidir ◽  
...  
Keyword(s):  
Risk Factors ◽  
Esrd Patient ◽  
Hemorrhagic Fever ◽  
Cross Sectional Study ◽  
Hantavirus Infection ◽  
Plasma Samples ◽  
Igg Antibodies ◽  
Sectional Study ◽  
Cross Sectional ◽  
Esrd Patients

Abstract Background Hantaviruses are enveloped negative sense RNA viruses that cause hemorrhagic fever with renal syndrome. This study aimed to identify the prevalence of Hantavirus IgG antibodies and possible risk factors for Hantaviruses infections among end-stage renal disease (ESRD) patients attending the Dr Salma dialysis center in Sudan. Methodology This was a cross-sectional study in which 91 ESRD patients and 30 healthy plasma samples were screened for Hantavirus IgG antibodies using ELISA. A questionnaire containing sociodemographics, history of rat exposure and clinical data information was filled in by each ESRD patient. Results In this study, 9 out of 91 ESRD patients (9.9%) tested positive for Hantaviruses antibodies (IgG) while none of the 30 healthy plasma samples showed seropositivity. There was no statistically significant association between age, gender, educational level and rat exposure and Hantavirus infection in ESRD patients (p&gt;0.05) Conclusion This study is the first to be conducted in Sudan regarding Hantaviruses and ESRD. The prevalence of Hantavirus antibodies among ESRD patients is high compared with findings reported in the literature from studies conducted on the same group of patients. It points to an interesting question as to whether Hantaviruses have an association with ESRD but further studies are needed before drawing any conclusions.

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