scholarly journals 2,4-Diacetylphloroglucinol Alters Plant Root Development

2008 ◽  
Vol 21 (10) ◽  
pp. 1349-1358 ◽  
Author(s):  
Jessica N. Brazelton ◽  
Emily E. Pfeufer ◽  
Teresa A. Sweat ◽  
Brian B. McSpadden Gardener ◽  
Catharina Coenen
Keyword(s):  
Root Growth ◽  
Plant Root ◽  
Primary Root ◽  
Luciferase Reporter ◽  
Root Production ◽  
Inhibitory Effects ◽  
Luciferase Reporter Gene ◽  
Root Branching ◽  
Tomato Seedlings ◽  
Primary Root Growth

Pseudomonas fluorescens isolates containing the phlD gene can protect crops from root pathogens, at least in part through production of the antibiotic 2,4-diacetylphloroglucinol (DAPG). However, the action mechanisms of DAPG are not fully understood, and effects of this antibiotic on host root systems have not been characterized in detail. DAPG inhibited primary root growth and stimulated lateral root production in tomato seedlings. Roots of the auxin-resistant diageotropica mutant of tomato demonstrated reduced DAPG sensitivity with regards to inhibition of primary root growth and induction of root branching. Additionally, applications of exogenous DAPG, at concentrations previously found in the rhizosphere of plants inoculated with DAPG-producing pseudomonads, inhibited the activation of an auxin-inducible GH3 promoter∷luciferase reporter gene construct in transgenic tobacco hypocotyls. In this model system, supernatants of 17 phlD+ P. fluorescens isolates had inhibitory effects on luciferase activity similar to synthetic DAPG. In addition, a phlD– mutant strain, unable to produce DAPG, demonstrated delayed inhibitory effects compared with the parent wild-type strain. These results indicate that DAPG can alter crop root architecture by interacting with an auxin-dependent signaling pathway.

Exogenous nitrate induces root branching and inhibits primary root growth in Capsicum chinense Jacq.

2011 ◽  
Vol 49 (12) ◽  
pp. 1456-1464 ◽  
Author(s):  
Teresita de Jesús Celis-Arámburo ◽  
Mildred Carrillo-Pech ◽  
Lizbeth A. Castro-Concha ◽  
María de Lourdes Miranda-Ham ◽  
Manuel Martínez-Estévez ◽  
...  
Keyword(s):  
Root Growth ◽  
Primary Root ◽  
Capsicum Chinense ◽  
Root Branching ◽  
Primary Root Growth

scholarly journals AtMYB44 Positively Regulates the Enhanced Elongation of Primary Roots Induced by N-3-Oxo-Hexanoyl-Homoserine Lactone in Arabidopsis thaliana

2016 ◽  
Vol 29 (10) ◽  
pp. 774-785 ◽  
Author(s):  
Qian Zhao ◽  
Man Li ◽  
Zhenhua Jia ◽  
Fang Liu ◽  
Hong Ma ◽  
...  
Keyword(s):  
Root Growth ◽  
Root Elongation ◽  
Plant Root ◽  
Primary Root ◽  
Homoserine Lactone ◽  
Defense Responses ◽  
Plant Response ◽  
Signal Molecules ◽  
Cellular Analysis ◽  
Primary Root Growth

N-acyl-homoserine lactones (AHL) are the quorum-sensing (QS) signal molecules used by many gram-negative bacteria to coordinate their collective behavior in a population. Recent evidence demonstrates their roles in plant root growth and defense responses. AtMYB44 is a multifaceted transcriptional factor that functions in many physiological processes in plants but whether AtMYB44 modulates the plant response to AHL with aspects of primary root elongation remains unknown. Here, we show that the expression of AtMYB44 was upregulated upon treatment with N-3-oxo-hexanoyl-homoserine lactone (3OC6-HSL). The stimulatory effect of 3OC6-HSL on primary root elongation was abolished in the AtMYB44 functional-deficiency mutant atmby44. In contrast, an enhanced promoting-impact of 3OC6-HSL on primary root growth was observed in AtMYB44-overexpressing plant MYB44OTA. Cellular analysis indicated that the prolonged primary root elicited by 3OC6-HSL is the consequence of increased cell division in the meristem zone and enhanced cell elongation in the elongation zone, and AtMYB44 may act as a positive regulator in this process. Furthermore, we demonstrated that AtMYB44 might participate in the 3OC6-HSL-mediated primary root growth via regulating the expression of cytokinin- and auxin-related genes. The data establish a genetic connection between the regulatory role of AtMYB44 in phytohormones-related gene expression and plant response to the bacterial QS signal.

scholarly journals High throughput phenotyping of root growth dynamics, lateral root formation, root architecture and root hair development enabled by PlaRoM

2009 ◽  
Vol 36 (11) ◽  
pp. 938 ◽  
Author(s):  
Nima Yazdanbakhsh ◽  
Joachim Fisahn
Keyword(s):  
Root Growth ◽  
Growth Kinetics ◽  
Lateral Root ◽  
Root Architecture ◽  
Imaging Techniques ◽  
Plant Root ◽  
Primary Root ◽  
Growth Media ◽  
Root Extension ◽  
Lateral Root Development

Plant organ phenotyping by non-invasive video imaging techniques provides a powerful tool to assess physiological traits and biomass production. We describe here a range of applications of a recently developed plant root monitoring platform (PlaRoM). PlaRoM consists of an imaging platform and a root extension profiling software application. This platform has been developed for multi parallel recordings of root growth phenotypes of up to 50 individual seedlings over several days, with high spatial and temporal resolution. PlaRoM can investigate root extension profiles of different genotypes in various growth conditions (e.g. light protocol, temperature, growth media). In particular, we present primary root growth kinetics that was collected over several days. Furthermore, addition of 0.01% sucrose to the growth medium provided sufficient carbohydrates to maintain reduced growth rates in extended nights. Further analysis of records obtained from the imaging platform revealed that lateral root development exhibits similar growth kinetics to the primary root, but that root hairs develop in a faster rate. The compatibility of PlaRoM with currently accessible software packages for studying root architecture will be discussed. We are aiming for a global application of our collected root images to analytical tools provided in remote locations.

scholarly journals Developmental Distribution of the Plasma Membrane-Enriched Proteome in the Maize Primary Root Growth Zone

2013 ◽  
Vol 4 ◽  
Author(s):  
Zhe Zhang ◽  
Priyamvada Voothuluru ◽  
Mineo Yamaguchi ◽  
Robert E. Sharp ◽  
Scott C. Peck
Keyword(s):  
Plasma Membrane ◽  
Root Growth ◽  
Primary Root ◽  
Growth Zone ◽  
Primary Root Growth

scholarly journals The Arabidopsis bZIP11 transcription factor links low-energy signalling to auxin-mediated control of primary root growth

PLoS Genetics ◽  
2017 ◽  
Vol 13 (2) ◽  
pp. e1006607 ◽  
Author(s):  
Christoph Weiste ◽  
Lorenzo Pedrotti ◽  
Jebasingh Selvanayagam ◽  
Prathibha Muralidhara ◽  
Christian Fröschel ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Root Growth ◽  
Primary Root ◽  
Low Energy ◽  
Primary Root Growth

scholarly journals Temperature and pH of the nutrient solution on wheat primary root growth

2004 ◽  
Vol 61 (3) ◽  
pp. 313-318 ◽  
Author(s):  
Carlos Eduardo de Oliveira Camargo ◽  
Antonio Wilson Penteado Ferreira Filho ◽  
Marcus Vinicius Salomon
Keyword(s):  
Root Growth ◽  
Nutrient Solution ◽  
Primary Root ◽  
Growth Period ◽  
Triticum Aestivum L ◽  
Solution Temperature ◽  
Stages Of Development ◽  
Primary Root Growth ◽  
Temperature And Growth ◽  
Nutrient Solutions

Primary root growth is very important for wheat (Triticum aestivum L.) crop in upland conditions in the State of São Paulo. Fourteen wheat genotypes (mutant lines and cultivars) were evaluated for primary root growth during 7 and 15 days of development in complete and aerated nutrient solutions, in the laboratory. In the first experiment, solutions with three pH values (4.0, 5.0 and 6.0) at constant temperature (24 ± 1°C), and in the second experiment, solutions with the same pH (4.0) but with three temperatures (18°C ± 1°C, 24°C ± 1°C and 30°C ± 1°C) were used. High genetic variability was observed among the evaluated genotypes in relation to primary root growth in the first stages of development in nutrient solutions independent of pH, temperature and growth period. Genotypes 6 (BH-1146) and 13 (IAC-17), tolerant to Al3+ showed genetic potential for root growth in the first stages of development (7 and 15 days), regardless of nutrient solution temperature and pH. Genotypes 14 (IAC-24 M), 15 (IAC-24), 17 (MON"S" / ALD "S") ´ IAC-24 M2, 18 (MON"S" / ALD "S") ´ IAC-24 M3 and 24 (KAUZ"S" / IAC-24 M3), tolerant to Al3+, showed reduced root growth under the same conditions.

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