scholarly journals Differential Display Analysis of the Early Compatible Interaction Between Soybean and the Soybean Cyst Nematode

1998 ◽  
Vol 11 (12) ◽  
pp. 1258-1263 ◽  
Author(s):  
Dieter Hermsmeier ◽  
Mitra Mazarei ◽  
Thomas J. Baum
Keyword(s):  
Gene Expression ◽  
Differential Display ◽  
Soybean Cyst Nematode ◽  
Cyst Nematode ◽  
Site Formation ◽  
Cdna Clones ◽  
Mrna Levels ◽  
Compatible Interaction ◽  
Feeding Site ◽  
Differential Display Analysis

The marked cellular changes during feeding site formation of the soybean cyst nematode (Heterodera glycines) indicate major changes in soybean gene expression. We used differential display of mRNA to detect host gene expression changes during the early compatible interaction between soybean and H. glycines. Fifteen cDNA clones corresponding to mRNAs with different abundances in H. glycines-infected versus uninfected roots were identified. Differential display results indicated that abundances of five mRNAs increased in infected roots, whereas abundances of 10 mRNAs decreased. Transcripts for nine of these 15 cDNAs could be detected on RNA blots, and their hybridization signals confirmed the differential display results for eight of these nine cDNAs. Sequence analyses identified five cDNAs with decreased mRNA levels in infected roots as corresponding to two putative aldolase genes, a transcription-factor TFIIA homologue, the soybean small GTP-binding protein gene sra1, and the soybean auxin down-regulated gene ADR12. RNA blot analyses of other auxin down-regulated genes revealed a decrease in their mRNA abundances in H. glycines-infected roots as well.

scholarly journals Modulations in Gene Expression and Mapping of Genes Associated with Cyst Nematode Infection of Soybean

2001 ◽  
Vol 14 (1) ◽  
pp. 42-54 ◽  
Author(s):  
Zarir Vaghchhipawala ◽  
Ronald Bassüner ◽  
Kathryn Clayton ◽  
Kimberley Lewers ◽  
Randy Shoemaker ◽  
...  
Keyword(s):  
Gene Expression ◽  
Soybean Cyst Nematode ◽  
Genetic System ◽  
Cyst Nematode ◽  
Infection Process ◽  
Resistance Locus ◽  
Root Cells ◽  
Feeding Site ◽  
Susceptible Line ◽  
Scn Resistance

Infection of the soybean root by the soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) induces a well-documented, yet poorly understood, response by the host plant. The plant response, involving the differentiation of a feeding structure, or “syncytium,” facilitates the feeding and reproduction of the nematode to the detriment of the host. We used a genetic system involving a single dominant soybean gene conferring susceptibility to an inbred nematode strain, VL1, to characterize the nematode-host interaction in susceptible line PI 89008. The restriction fragment length polymorphism marker pB053, shown to map to a major SCN resistance locus, cosegregates with resistance among F2 progeny from the PI 89008 × PI 88287 cross. Cytological examination of the infection process confirmed that syncytium development in this genetic system is similar to that reported by others who used noninbred nematode lines. Our study of infected root tissue in the susceptible line PI 89008 revealed a number of genes enhanced in expression. Among these are catalase, cyclin, elongation factor 1α, β-1,3-endoglucanase, hydroxymethylglutaryl coenzyme A reductase, heat shock protein 70, late embryonic abundant protein 14, and formylglycinamidine ribonucleotide synthase, all of which we have genetically positioned on the public linkage map of soybean. Formylglycinamidine ribonucleotide synthase was found to be tightly linked with a major quantitative trait locus for SCN resistance. Our observations are consistent with the hypothesis proposed by others that feeding site development involves the dramatic modulation of gene expression relative to surrounding root cells.

scholarly journals Parallel Genome-Wide Expression Profiling of Host and Pathogen During Soybean Cyst Nematode Infection of Soybean

2007 ◽  
Vol 20 (3) ◽  
pp. 293-305 ◽  
Author(s):  
Nagabhushana Ithal ◽  
Justin Recknor ◽  
Dan Nettleton ◽  
Leonard Hearne ◽  
Tom Maier ◽  
...  
Keyword(s):  
Gene Expression ◽  
Differential Expression ◽  
Soybean Cyst Nematode ◽  
Defense Responses ◽  
Cyst Nematode ◽  
Nematode Infection ◽  
Primary Metabolism ◽  
Compatible Interaction ◽  
Significant Differential Expression ◽  
Genome Wide

Global analysis of gene expression changes in soybean (Glycine max) and Heterodera glycines (soybean cyst nematode [SCN]) during the course of infection in a compatible interaction was performed using the Affymetrix GeneChip soybean genome array. Among 35,611 soybean transcripts monitored, we identified 429 genes that showed statistically significant differential expression between uninfected and nematode-infected root tissues. These included genes encoding enzymes involved in primary metabolism; biosynthesis of phenolic compounds, lignin, and flavonoids; genes related to stress and defense responses; cell wall modification; cellular signaling; and transcriptional regulation. Among 7,431 SCN transcripts monitored, 1,850 genes showed statistically significant differential expression across different stages of nematode parasitism and development. Differentially expressed SCN genes were grouped into nine different clusters based on their expression profiles during parasitism of soybean roots. The patterns of gene expression we observed in SCN suggest coordinated regulation of genes involved in parasitism. Quantitative real-time reverse-transcription polymerase chain reaction confirmed the results of our microarray analysis. The simultaneous genome-wide analysis of gene expression changes in the host and pathogen during a compatible interaction provides new insights into soybean responses to nematode infection and the first profile of transcript abundance changes occurring in the nematode as it infects and establishes a permanent feeding site within a host plant root.

Gene expression profile of human bone marrow stromal cells determined by restriction fragment differential display analysis

2004 ◽  
Vol 92 (4) ◽  
pp. 733-744 ◽  
Author(s):  
Massimiliano Monticone ◽  
Yi Liu ◽  
Laura Tonachini ◽  
Maddalena Mastrogiacomo ◽  
Stefano Parodi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Marrow ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Restriction Fragment ◽  
Differential Display ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Differential Display Analysis

Plasmodium falciparum: Differential Display Analysis of Gene Expression during Gametocytogenesis

2001 ◽  
Vol 99 (4) ◽  
pp. 244-254 ◽  
Author(s):  
Liwang Cui ◽  
Kimberly A. Rzomp ◽  
Qi Fan ◽  
Samuel K. Martin ◽  
Jackie Williams
Keyword(s):  
Gene Expression ◽  
Plasmodium Falciparum ◽  
Differential Display ◽  
Differential Display Analysis

Differential display analysis of gene expression in female-to-male sex-reversing gonads of the frog Rana rugosa

2008 ◽  
Vol 155 (3) ◽  
pp. 623-634 ◽  
Author(s):  
Goro Okada ◽  
Koichi Maruo ◽  
Sadahiro Funada ◽  
Masahisa Nakamura
Keyword(s):  
Gene Expression ◽  
Differential Display ◽  
Differential Display Analysis ◽  
Male Sex

Gene expression in the rat supraoptic nucleus induced by chronic hyperosmolality versus hyposmolality

2000 ◽  
Vol 279 (4) ◽  
pp. R1239-R1250 ◽  
Author(s):  
Eric Glasgow ◽  
Takashi Murase ◽  
Bingjun Zhang ◽  
Joseph G. Verbalis ◽  
Harold Gainer
Keyword(s):  
Gene Expression ◽  
Supraoptic Nucleus ◽  
Cdna Libraries ◽  
Global Changes ◽  
Cdna Clones ◽  
Mrna Levels ◽  
Magnocellular Neurons ◽  
In Situ Hybridization Histochemistry ◽  
Functional Classes

Magnocellular neurons of the hypothalamo-neurohypophysial system play a fundamental role in the maintenance of body homeostasis by secreting vasopressin and oxytocin in response to systemic osmotic perturbations. During chronic hyperosmolality, vasopressin and oxytocin mRNA levels increase twofold, whereas, during chronic hyposmolality, these mRNA levels decrease to 10–20% of that of normoosmolar control animals. To determine what other genes respond to these osmotic perturbations, we have analyzed gene expression during chronic hyper- versus hyponatremia. Thirty-seven cDNA clones were isolated by differentially screening cDNA libraries that were generated from supraoptic nucleus tissue punches from hyper- or hyponatremic rats. Further analysis of 12 of these cDNAs by in situ hybridization histochemistry confirmed that they are osmotically regulated. These cDNAs represent a variety of functional classes and include cytochrome oxidase, tubulin, Na+-K+-ATPase, spectrin, PEP-19, calmodulin, GTPase, DnaJ-like, clathrin-associated, synaptic glycoprotein, regulator of GTPase stimulation, and gene for oligodendrocyte lineage-myelin basic proteins. This analysis therefore suggests that adaptation to chronic osmotic stress results in global changes in gene expression in the magnocellular neurons of the supraoptic nucleus.

Localization of hydrogen peroxide during the defence response of Arabidopsis thaliana against the plant-parasitic nematode Heterodera glycines

Nematology ◽  
1999 ◽  
Vol 1 (7) ◽  
pp. 681-686 ◽  
Author(s):  
Georg Waetzig ◽  
Miroslaw Sobczak ◽  
Florian Grundler
Keyword(s):  
Hydrogen Peroxide ◽  
Arabidopsis Thaliana ◽  
Soybean Cyst Nematode ◽  
Parasitic Nematode ◽  
Heterodera Glycines ◽  
Cyst Nematode ◽  
Defence Response ◽  
H2o2 Production ◽  
Cerium Chloride ◽  
Feeding Site

AbstractHydrogen peroxide (H2O2) production during the infection of Arabidopsis thaliana by the soybean cyst nematode Heterodera glycines was detected histochemically by the reaction of H2O2 with cerium chloride producing four different patterns of electron-dense precipitates of cerium perhydroxides. As A. thaliana is not a regular host of H. glycines, the defence response is considerable, but does not completely inhibit the development of the nematode. H2O2 was produced not only by cells mechanically damaged during invasion and feeding site induction by the nematode, but also by cells surrounding developing syncytia and cells which were neither in contact with the nematode nor with the syncytium. Die Lokalisation von Peroxid wahrend der Abwehrreaktion von Arabidopsis thaliana gegen den pflanzenparasitaren Nematoden Heterodera glycines - Die Bildung von Wasserstoffperoxid (H2O2) im Rahmen der Infektion von Arabidopsis thaliana durch den Sojabohnen-Zystennematoden Heterodera glycines wurde histochemisch durch die Reaktion von H2O2 mit Cerchlorid nachgewiesen, wobei vier verschiedene Muster elektronendichter Prazipitate von Cerperhydroxiden gebildet wurden. Da A. thaliana kein regularer Wirt von H. glycines ist, kommt es zu einer betrachtlichen Abwehrreaktion, die jedoch die Entwicklung des Nematoden nicht vollstandig verhindert. H2O2 wurde nicht nur von Zellen produziert, die im Laufe des Eindringens und der Induktion des Nahrzellensystems durch den Nematoden mechanisch beschadigt worden waren, sondern auch von Zellen, die sich entwickelnde Syncytien umgaben und von Zellen, die weder mit dem Nematoden noch mit dem Syncytium in Kontakt standen.

Sign in / Sign up

Export Citation Format

Share Document