scholarly journals Trichothecene Genotype of Fusarium graminearum Isolates from Soybean (Glycine max) Seedling and Root Diseases in the United States

Plant Disease ◽  
2014 ◽  
Vol 98 (7) ◽  
pp. 1012-1012 ◽  
Author(s):  
M. L. Ellis ◽  
G. P. Munkvold
Keyword(s):  
United States ◽  
North America ◽  
Fusarium Graminearum ◽  
Root Rot ◽  
Elongation Factor ◽  
Host Shift ◽  
The United States ◽  
Sensu Stricto ◽  
Head Blight ◽  
The Third

Fusarium graminearum is an economically important pathogen that causes Fusarium head blight of wheat, barley, and oat, and Gibberella ear and stalk rot of maize. More recently, F. graminearum was reported as a soybean seedling and root pathogen in North America (1,5), causing seed decay, damping-off, and brown to reddish-brown root rot symptoms. Type B trichothecene mycotoxins are commonly produced by F. graminearum, which can be categorized into three trichothecene genotypes; those that produce 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), or nivalenol (NIV). The 15-ADON genotype is dominant in populations from small grains and maize in North America (4), but the 3-ADON genotype has recently been found (4). F. graminearum was known as a pathogen of wheat and maize in North America for over a century before it was reported as a soybean pathogen. Therefore, we hypothesized that recent reports on soybean could be associated with the appearance of the 3-ADON genotype. The objective of this research was to determine the trichothecene genotype of F. graminearum isolates from soybean in the United States. Thirty-eight isolates from soybean were evaluated. Twenty-seven isolates came from a 3-year survey for Fusarium root rot from 2007 to 2009 in Iowa. Other isolates (Ahmad Fakhoury, Southern Illinois University, Carbondale) were collected from soybean seedlings during a multi-state survey in 2012, and included three isolates from Illinois, three from Indiana, and five from Nebraska. Species identification and lineage of F. graminearum were confirmed by sequencing the translation elongation factor gene (EF1-α) using EF-1H and EF-2T primers. A maximum likelihood analysis of the EF1-α, including voucher strains from nine lineages of F. graminearum (2), placed all 38 isolates into lineage 7, F. graminearum sensu stricto (representative GenBank accessions KJ415349 to KJ415352). To determine the trichothecene genotype of each isolate we used three multiplex PCR assays. The first two assays targeted a portion of trichothecene biosynthesis genes Tri3 and Tri12 (4), while the third assay targeted portions of the Tri3, Tri5, and Tri7 genes (3). The PCR for the first two assays was conducted as described by Ward et al. (4) using four sets of primers: 3CON, 3NA, 3D15A, and 3D3A; and 12CON, 12NF, 12-15F, and 12-3F for the Tri3 and Tri12 genes, respectively. The PCR for the third assay was conducted as described by Quarta et al. (3) using the following primers: Tri3F971, Tri3F1325, Tri3R1679, Tri7F340, Tri7R965, 3551H, and 4056H. The amplification products were analyzed by gel electrophoresis. All 38 isolates produced amplicons consistent with the 15-ADON genotype; ~610 and 670 bp for the Tri3 and Tri12 genes, respectively (4), and two amplicons of ~708 and 525 bp for the Tri3/Tri5 genes (3). Our results indicated that the dominant trichothecene genotype among isolates of F. graminearum from soybean is 15-ADON, and the introduction of 3-ADON isolates does not explain the recent host shift of F. graminearum to soybean in North America. To our knowledge, this is the first assessment of trichothecene genotypes in F. graminearum populations from soybean from the United States. References: (1) K. E. Broders et al. Plant Dis. 91:1155, 2007. (2) K. O'Donnell et al. Fungal Gen. Biol. 41:600, 2004. (3) A. Quarta et al. FEMS Microbiol. Lett. 259:7, 2006. (4) T. D. Ward et al. Fungal Gen. Biol. 45:473, 2008. (5) A. G. Zue et al. Can. J. Plant Pathol. 29:35, 2007.

scholarly journals Nivalenol-Type Populations of Fusarium graminearum and F. asiaticum Are Prevalent on Wheat in Southern Louisiana

2011 ◽  
Vol 101 (1) ◽  
pp. 124-134 ◽  
Author(s):  
Liane Rosewich Gale ◽  
Stephen A. Harrison ◽  
Todd J. Ward ◽  
Kerry O'Donnell ◽  
Eugene A. Milus ◽  
...  
Keyword(s):  
United States ◽  
North Carolina ◽  
Fusarium Graminearum ◽  
Gulf Coast ◽  
Pcr Primers ◽  
The United States ◽  
Sensu Stricto ◽  
Head Blight ◽  
Trichothecene Mycotoxin ◽  
Southern Louisiana

U.S. populations of the Fusarium graminearum clade cause head blight on wheat and barley and usually contaminate grain with the trichothecene mycotoxin deoxynivalenol (DON). Recently, however, individual nivalenol (NIV)-type isolates from the United States were described that belonged to either the newly described species F. gerlachii or the genetically distinct Gulf Coast population of F. graminearum sensu stricto (s.s.). Here, we describe the discovery of NIV-type F. graminearum s.s. populations that were found in high proportion (79%) among isolates from small-grain-growing regions of Louisiana. We genotyped 237 isolates from Louisiana with newly developed polymerase chain reaction (PCR) restriction fragment length polymorphism markers and multiplex PCR primers that distinguish among the three trichothecene types: the two DON types (15ADON and 3ADON) and NIV. These isolates were compared with 297 isolates from 11 other U.S. states, predominantly from the Midwest. Using Bayesian-model-based clustering, we discovered a southern Louisiana population of F. graminearum s.s. that was genetically distinct from the previously recognized pathogen population in the Midwest (MW15ADON population). Population membership was correlated with trichothecene type. Most isolates from the southern Louisiana population were of the NIV type, while the majority of the isolates from the Midwest were of the 15ADON type. A smaller proportion of isolates from Louisiana belonged to the previously described Gulf Coast population that was mostly of the 3ADON type. The NIV type was also identified in collections from Arkansas (12%), North Carolina (40%), and Missouri (2%), with the collections from Arkansas and North Carolina being small and unrepresentative. F. asiaticum was detected from the two southern Louisiana parishes Acadia and Alexandria. All identified 41 F. asiaticum isolates were of the NIV type. Greenhouse tests indicated that U.S. NIV types accumulated four times less trichothecene toxin than DON types on inoculated wheat. This is the first report of NIV-type populations of F. graminearum s. s. and F. asiaticum in the United States.

scholarly journals Resistance in Winter Wheat Lines to Initial Infection and Spread Within Spikes by Deoxynivalenol and Nivalenol Chemotypes of Fusarium graminearum

Plant Disease ◽  
2011 ◽  
Vol 95 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Peter Horevaj ◽  
Liane R. Gale ◽  
Eugene A. Milus
Keyword(s):  
United States ◽  
Winter Wheat ◽  
Fusarium Graminearum ◽  
The United States ◽  
Blight Resistance ◽  
Head Blight ◽  
Initial Infection ◽  
Additional Information ◽  
Progress Curve ◽  
Wheat Lines

Head blight of wheat in the United States is caused primarily by the deoxynivalenol (DON)-producing chemotype of Fusarium graminearum. However, the discovery of the nivalenol (NIV) chemotype of F. graminearum in Louisiana and Arkansas necessitates having resistance in wheat to both chemotypes. The objectives of this research were to quantify resistance of selected winter wheat lines to initial infection and pathogen spread within spikes, to determine whether wheat lines selected for resistance to the DON chemotype also have resistance to the NIV chemotype, and to improve the methods for quantifying resistance to initial infection. A susceptible check (Coker 9835) and 15 winter wheat lines, which are adapted to the southeastern United States and possess diverse sources of head blight resistance, were evaluated for head blight resistance in a series of greenhouse and growth-chamber experiments. Significant levels of resistance to both initial infection and spread within a spike were found among the lines, and lines with resistance to isolates of the DON chemotype had even higher levels of resistance to isolates of the NIV chemotype. Quantifying resistance to initial infection was improved by standardizing the inoculum and environmental conditions. Additional information related to resistance to spread within a spike was obtained by calculating the area under the disease progress curve from 7 to 21 days after inoculation.

scholarly journals First Report of Puccinia coronata var. coronata sensu stricto Infecting Alder Buckthorn in the United States

2017 ◽  
Vol 18 (2) ◽  
pp. 84-86
Author(s):  
Shawn C. Kenaley ◽  
Geoffrey Ecker ◽  
Gary C. Bergstrom
Keyword(s):  
United States ◽  
North America ◽  
Host Range ◽  
Geographic Distribution ◽  
Phylogenetic Analyses ◽  
Rust Fungus ◽  
The United States ◽  
Sensu Stricto ◽  
Puccinia Coronata ◽  
First Report

Field symptoms, host distribution, pathogen morphology, and phylogenetic analyses clearly demonstrated that the rust fungus infecting alder buckthorn in Connecticut is Puccinia coronata var. coronata sensu stricto. To our knowledge, this is the first report and confirmation of P. coronata var. coronata s.s. in the United States. Additional collections from purported aecial and telial hosts of P. coronata var. coronata s.s. are necessary to determine its host range, geographic distribution, and incidence within the United States and elsewhere in North America.

scholarly journals Specific Detection and Identification of Fusarium graminearum Sensu Stricto Using a PCR-RFLP Tool and Specific Primers Targeting the Translational Elongation Factor 1α Gene

Plant Disease ◽  
2020 ◽  
Vol 104 (4) ◽  
pp. 1076-1086
Author(s):  
Mohamed Hafez ◽  
Ahmed Abdelmagid ◽  
Lorne R. Adam ◽  
Fouad Daayf
Keyword(s):  
Fusarium Graminearum ◽  
Elongation Factor ◽  
Sensu Stricto ◽  
Restriction Pattern ◽  
Head Blight ◽  
Specific Detection ◽  
Fusarium Spp ◽  
Elongation Factor 1Α ◽  
Detection And Identification ◽  
Unique Restriction

Fusarium graminearum is a toxigenic plant pathogen that causes Fusarium head blight (FHB) disease on cereal crops. It has recently shown to have cross-pathogenicity on noncereals (i.e., Fusarium root rot [FRR] on soybean) in Canada and elsewhere. Specific detection and differentiation of this potent toxigenic, trichothecene-producing pathogen among other closely related species is extremely important for disease control and mycotoxin monitoring. Here, we designed a PCR restriction fragment length polymorphism protocol based on the DNA sequence of the translational elongation factor 1α (TEF1α) gene. A unique restriction site to the enzyme HpaII is only found in F. graminearum sensu stricto strains among different Fusarium strains in the F. graminearum species complex (FGSC) and other Fusarium spp. associated with FHB in cereals and FRR in soybean. Partial amplification of the TEF1α gene with newly designed primers mh1/mh2 generated a 459-bp PCR fragment. Restriction digestion of the generated fragments with the HpaII enzyme generated a unique restriction pattern that can rapidly and accurately differentiate F. graminearum sensu stricto among all other Fusarium spp. A primer pair (FgssF/FgssR) specific to F. graminearum sensu stricto also was designed and can distinguish F. graminearum sensu stricto from all other Fusarium spp. in the FGSC and other closely related Fusarium spp. involved in FHB and FRR. This finding will be very useful for the specific detection of F. graminearum sensu stricto for diagnostic purposes as well as for the accurate detection of this pathogen in breeding and other research purposes.

The Galium aparine complex in Canada

1975 ◽  
Vol 53 (9) ◽  
pp. 877-893 ◽  
Author(s):  
R. J. Moore
Keyword(s):  
United States ◽  
North America ◽  
Chromosome Number ◽  
The United States ◽  
Sensu Stricto ◽  
Western Canada ◽  
Galium Aparine ◽  
Prairie Provinces

The occurrence in Canada of Galium aparine sensu lato has been studied. It is concluded that two species are present: G. aparine L. sensu stricto (Cleavers) and G. spurium L. (False Cleavers). Although closely related and morphologically similar, these species differ morphologically, cytologically, and physiologically. The chromosome number of collections of G. aparine from Canada and the United States was found to be 2n = 66, that of G. spurium from the prairie provinces of Canada was 2n = 20. The latter species is an introduction from Eurasia and seems to be the more serious weed in fields of grain and rapeseed in western Canada. Galium aparine is believed to be native, as well as introduced from Eurasia, in North America. Galium aparine occurs usually in moist, shaded habitats, whereas G. spurium favors sunnier habitats. Both species have spiny or smooth fruits and these fruit variants have been recognized at various taxonomic levels. In this paper they are reduced to the rank of form.

Disk and Dat: Recent Developments in Legal Databases and Emerging Information Technologies in the United States

1987 ◽  
Vol 15 (3-4) ◽  
pp. 109-123
Author(s):  
Howard A. Hood
Keyword(s):  
United States ◽  
North America ◽  
Information Technologies ◽  
The United States ◽  
Computer Assisted ◽  
Legal Research ◽  
The Third ◽  
Recent Developments ◽  
Legal Databases

This is the third installment in a series of studies which began in 1983. The earlier essays surveyed computer-assisted legal research in Europe, the Commonwealth, and North America. The present study will concentrai on developments in the United States.

scholarly journals Evolutionary Aspects of Emerging Lyme Disease in Canada

2015 ◽  
Vol 81 (21) ◽  
pp. 7350-7359 ◽  
Author(s):  
N. H. Ogden ◽  
E. J. Feil ◽  
P. A. Leighton ◽  
L. R. Lindsay ◽  
G. Margos ◽  
...  
Keyword(s):  
Public Health ◽  
United States ◽  
At Risk ◽  
North America ◽  
Lyme Disease ◽  
Severe Disease ◽  
The United States ◽  
Sensu Stricto ◽  
Content Type ◽  
Evolutionary Aspects

ABSTRACTIn North America, Lyme disease (LD) is a tick-borne zoonosis caused by the spirochete bacteriumBorrelia burgdorferisensu stricto, which is maintained by wildlife. Tick vectors and bacteria are currently spreading into Canada and causing increasing numbers of cases of LD in humans and raising a pressing need for public health responses. There is no vaccine, and LD prevention depends on knowing who is at risk and informing them how to protect themselves from infection. Recently, it was found in the United States that some strains ofB. burgdorferisensu strictocause severe disease, whereas others cause mild, self-limiting disease. While many strains occurring in the United States also occur in Canada, strains in some parts of Canada are different from those in the United States. We therefore recognize a need to identify which strains specific to Canada can cause severe disease and to characterize their geographic distribution to determine which Canadians are particularly at risk. In this review, we summarize the history of emergence of LD in North America, our current knowledge ofB. burgdorferisensu strictodiversity, its intriguing origins in the ecology and evolution of the bacterium, and its importance for the epidemiology and clinical and laboratory diagnosis of LD. We propose methods for investigating associations betweenB. burgdorferisensu strictodiversity, ecology, and pathogenicity and for developing predictive tools to guide public health interventions. We also highlight the emergence ofB. burgdorferisensu strictoin Canada as a unique opportunity for exploring the evolutionary aspects of tick-borne pathogen emergence.

Pathogenic specialization in Fusarium graminearum

1971 ◽  
Vol 22 (4) ◽  
pp. 553 ◽  
Author(s):  
GS Purss
Keyword(s):  
United States ◽  
Fusarium Graminearum ◽  
The United States ◽  
Crown Rot ◽  
Foot Rot ◽  
Significant Extent ◽  
Head Blight ◽  
Stalk Rot ◽  
Specialized Form

Isolates of Fusarium graminearum from crown rot of wheat, stalk rot of maize (both in Australia and in the United States), and head blight of wheat are all shown to be capable of causing head blight of wheat to a significant level. Stalk rot of maize is shown to be caused by isolates of F. graminearum from stalk rot of maize, crown rot of wheat, and head blight of wheat. Isolates of F. culmorum from foot rot of wheat and barley are also capable of causing stalk rot of maize. Typical crown rot of wheat is shown to be caused to a significant extent only by the isolates from this disease syndrome. The conclusion is drawn that a specialized form of F. graminearurn is responsible for crown rot in wheat.

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