scholarly journals Characterization of Colletotrichum spp. Sensitivity to Carbendazim for Isolates Causing Strawberry Anthracnose in China

Plant Disease ◽  
2021 ◽  
Vol 105 (1) ◽  
pp. 87-95
Author(s):  
Shan Zhong ◽  
Jianqiang Miao ◽  
Xili Liu ◽  
Guozhen Zhang
Keyword(s):  
Point Mutation ◽  
Morphological Characteristics ◽  
Economic Losses ◽  
Sequence Analyses ◽  
Colletotrichum Species ◽  
Strawberry Anthracnose ◽  
Colletotrichum Spp ◽  
Moderately Resistant ◽  
Important Disease

Strawberry anthracnose caused by Colletotrichum species is an important disease that may cause significant economic losses. Based on multilocus sequence analyses and morphological characteristics, 64 isolates from strawberry anthracnose samples collected from nine Chinese provinces and municipalities were identified as three species: Colletotrichum fructicola (29 isolates), Colletotrichum siamense (23 isolates), and Colletotrichum nymphaeae (12 isolates). Isolates of C. siamense showed strong aggressiveness to fruit and leaves. Isolates of C. fructicola showed strong aggressiveness to crowns. Isolates of C. nymphaeae were weakly or not pathogenic to fruit, leaves, or crowns. Sensitivity to carbendazim was determined for a total of 75 isolates, including 11 previously preserved. Two isolates of C. siamense were highly resistant (HR) and 21 were moderately resistant (MR). Nine isolates of C. fructicola were sensitive (S), 24 were HR, and four were MR. All 15 isolates of C. nymphaeae were insensitive, and their mycelial growth was not completely inhibited on potato dextrose agar amended with 500 μg/ml carbendazim. Beta-tubulin (TUB2) of representative isolates was amplified and sequenced, revealing a glutamic acid substituted by alanine at codon 198 in HR isolates of C. siamense and C. fructicola. MR isolates of C. siamense and C. fructicola had a point mutation at codon 200, causing a replacement of phenylalanine acid by tyrosine. No point mutation was detected at codons 50, 167, 198, 200, or 240 in TUB2 of C. nymphaeae insensitive isolates. Overall, this study revealed that C. fructicola was the dominant species causing anthracnose on strawberry and could improve the understanding of the management of fungicide resistance in Colletotrichum species on strawberry in China.

Identification and characterization of Colletotrichum species associated with Camellia sinensis anthracnose in Anhui province, China

Plant Disease ◽  
2020 ◽  
Author(s):  
Lei Zhang ◽  
Xianghan Li ◽  
YuanYuan Zhou ◽  
Genjia Tan ◽  
Lixin Zhang
Keyword(s):  
Camellia Sinensis ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Anhui Province ◽  
Tea Plantation ◽  
Colletotrichum Species ◽  
Pathogenicity Tests ◽  
Colletotrichum Spp ◽  
Identification And Characterization

Recent advances in Colletotrichum taxonomy have led to the need to conduct fresh surveys of Colletotrichum species associated with important crops. Anthracnose caused by Colletotrichum spp., is one of the destructive diseases on Camellia sinensis. In this study, a total of 22 representative Colletotrichum isolates were obtained from diseased leaves of Ca. sinensis cultivated in four tea plantation regions in Anhui province of China. The isolates were identified based on multi-locus (ITS, ACT, CAL, CHS-1, TUB2, GAPDH) phylogenetic analyses, and their morphological characteristics were also analyzed. Twenty-one isolates belonging to C. gloeosporioides complex were identified as C. camelliae, C. fructicola and C. siamense. One isolate belonging to C. boninense complex was identified as C. karstii. Pathogenicity tests revealed that the isolates of C. camelliae and C. fructicola were highly virulent when inoculated on the leaves of detached twigs of Ca. sinensis cv. Shuchazao. Furthermore, it was found that the interspecies virulence was less distinct and individual isolates showed varied virulence when inoculated on different varieties of Ca. sinensis. To our knowledge, this is the first report of C. fructicola, C. siamense and C. karstii causing anthracnose on Ca. sinensis in Anhui province, China.

scholarly journals Identification of Colletotrichum Species Associated with Blueberry Anthracnose in Sichuan, China

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 718
Author(s):  
Xuan Liu ◽  
Xiaojuan Zheng ◽  
Muhammad Ibrahim Khaskheli ◽  
Xiaofang Sun ◽  
Xiaoli Chang ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Economic Losses ◽  
Single Spore ◽  
Purification Method ◽  
Colletotrichum Fructicola ◽  
Pathogenicity Tests ◽  
Colletotrichum Spp ◽  
Important Disease

Anthracnose caused by Colletotrichum spp. is an important disease of blueberries and results in large economic losses for blueberry growers. Samples of anthracnose were collected from six main blueberry cultivation areas in Sichuan Province. In total, 74 Colletotrichum isolates were obtained through a single-spore purification method and identified to the species through morphological characteristics and phylogenetic analyses based on partial DNA sequences of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), internal transcribed spacer (ITS) regions, and the β-tubulin (TUB2), actin (ACT) and calmodulin (CAL) genes. Among all species, Colletotrichum fructicola was the most dominant species, with an isolation percentage of up to 66.2% in Sichuan, followed by Colletotrichum siamense (17.6%), C. kahawae (5.4%), C. karstii (5.4%), C. nymphaeae (2.7%) and C. sichuaninese (2.7%). Pathogenicity tests showed all species were able to cause typical anthracnose symptoms on blueberry leaves and stems. Colletotrichum fructicola was the predominant species with strong aggressiveness. Moreover, C. fructicola, C. kahawae, C. sichuaninese and C. nymphaeae are first reported here to cause blueberry anthracnose. This study provides a comprehensive reference for the association of different Colletotrichum spp., which may support the sustainable management of blueberry anthracnose.

scholarly journals Diversity of Colletotrichum Species Associated with Anthracnose Disease in Tropical Fruit Crops—A Review

Agriculture ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 297
Author(s):  
Latiffah Zakaria
Keyword(s):  
Management Strategies ◽  
Economic Losses ◽  
Fruit Crops ◽  
Tropical Fruit ◽  
Colletotrichum Species ◽  
Adequate Knowledge ◽  
Dragon Fruit ◽  
Causative Agents ◽  
Colletotrichum Spp ◽  
Fruit Plantations

In tropical fruit crops, anthracnose is mainly caused by species belonging to the fungal genus, Colletotrichum. These phytopathogens can infect several parts of the fruit crops; however, infection during postharvest or ripening stages is responsible for major economic losses. Due to the formation of black to dark brown sunken lesions on the fruit surface, anthracnose reduces fruit quality and marketability. Among the most common tropical fruit crops susceptible to anthracnose are mango, papaya, banana, avocado, guava, and dragon fruit; these are economically relevant products in many developing countries. It is important to document that the newly recorded Colletotrichum spp. associated with fruit anthracnose can infect multiple hosts, but some species may be host-specific. By using multiple markers, many phylogenetic species of Colletotrichum have been reported as anthracnose-causing pathogens. Taking into account that disease management strategies strongly rely on adequate knowledge of the causative agents, updated information on Colletotrichum species and the hazard posed by the most recently identified species in tropical fruit plantations and harvested fruits becomes vital. Besides, the newly recorded species may be important for biosecurity and should be listed as quarantine pathogens, considering that tropical fruits are traded worldwide.

scholarly journals Identification and Characterization of Benomyl-Resistant and -Sensitive Populations of Colletotrichum gloeosporioides from Statice (Limonium spp.)

2006 ◽  
Vol 96 (5) ◽  
pp. 542-548 ◽  
Author(s):  
Marcel Maymon ◽  
Aida Zveibil ◽  
Shimon Pivonia ◽  
Dror Minz ◽  
Stanley Freeman
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Sequence Analyses ◽  
Specific Primers ◽  
Tubulin Genes ◽  
Colletotrichum Spp ◽  
Polymerase Chain ◽  
Species Specific ◽  
Identification And Characterization ◽  
Propagation Material

Sixty-four isolates of Colletotrichum gloeosporioides were isolated from infected Limonium spp. cultivated in 12 different locations in Israel. All isolates were identified as belonging to the C. gloeosporioides complex by species-specific primers. Of these isolates, 46 were resistant to benomyl at 10 μg/ml and 18 were sensitive to this concentration of fungicide. Based on arbitrarily primed polymerase chain reaction of all isolates and internal transcribed spacer-1 sequence analyses of 12 selected isolates, the benomyl-resistant and -sensitive populations belong to two distinct genotypes. Sequence analyses of the β-tubulin genes, TUB1 and TUB2, of five sensitive and five resistant representative isolates of C. gloeosporioides from Limonium spp. revealed that the benomyl-resistant isolates had an alanine substitute instead of a glutamic acid at position 198 in TUB2. All data suggest that the resistant and sensitive genotypes are two independent and separate populations. Because all Limonium plant propagation material is imported from various geographic regions worldwide, and benomyl is not applied to this crop or for the control of Colletotrichum spp. in Israel, it is presumed that plants are bearing quiescent infections from the points of origin prior to arrival.

scholarly journals Genetic Diversity of Colletotrichum spp. Causing Strawberry Anthracnose in Zhejiang, China

Plant Disease ◽  
2020 ◽  
Vol 104 (5) ◽  
pp. 1351-1357 ◽  
Author(s):  
X. Y. Chen ◽  
D. J. Dai ◽  
S. F. Zhao ◽  
Y. Shen ◽  
H. D. Wang ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Dominant Species ◽  
Colletotrichum Gloeosporioides ◽  
Morphological Characteristics ◽  
Zhejiang Province ◽  
Control Measures ◽  
Chitin Synthase ◽  
Colletotrichum Gloeosporioides Complex ◽  
Strawberry Anthracnose ◽  
Colletotrichum Spp

Anthracnose is a serious fungal disease that primarily infects strawberry roots and stolons during development. Here, 91 isolates from different areas of Zhejiang province, China, were collected. Morphological characteristics were analyzed, and a phylogenetic analysis based on multiple genes (actin, internal transcribed spacer, calmodulin, glyceraldehyde-3-phosphate dehydrogenase, and chitin synthase) was performed. We found that all of the Colletotrichum species causing strawberry anthracnose belonged to the Colletotrichum gloeosporioides complex. Among them, we identified 48 isolates of C. fructicola, 21 isolates of C. siamense, 13 isolates of C. gloeosporioides, and 9 isolates of C. aenigma. C. siamense was distributed in the central and eastern regions of Zhejiang province (Hangzhou, Jinhua, Shaoxing, Ningbo, and Taizhou). This is the first report of C. siamense causing strawberry anthracnose in Zhejiang province. C. fructicola was the most dominant species causing strawberry anthracnose in Zhejiang province. We identified the four species causing strawberry anthracnose in Zhejiang province, which will improve our understanding of the strawberry anthracnose epidemic and will benefit the development of future control measures.

Resistance to azoxystrobin and thiophanate-methyl is widespread in Colletotrichum spp. isolates from the Mid-Atlantic Strawberry Fields

Plant Disease ◽  
2020 ◽  
Author(s):  
Qiuchen Luo ◽  
Anita Schoeneberg ◽  
Mengjun Hu
Keyword(s):  
Cyt B ◽  
Thiophanate Methyl ◽  
Colletotrichum Species ◽  
Predominant Species ◽  
Strawberry Anthracnose ◽  
Colletotrichum Spp ◽  
Moderate Resistance ◽  
High Level ◽  
Host Tissues

Multiple Colletotrichum species have been found to be responsible for strawberry anthracnose, and prevalence of each species seems to vary by regions and/or host tissues. In this study, a total of 200 Colletotrichum isolates were obtained from different strawberry cultivars displaying anthracnose symptoms in the Mid-Atlantic fields. Analysis of g3pdh, tub2, and/or ITS sequences revealed four Colletotrichum species, including C. nymphaeae, C. fioriniae, C. siamense, and C. lineola. C. nymphaeae was the predominant species, representing 90% of all isolates collected. This species was found from all strawberry organs/tissues examined, whereas C. siamense and C. fioriniae were limited to the crown and fruit, respectively. Further, all Colletotrichum isolates were screened for resistance to azoxystrobin in vitro, and all C. siamense isolates were additionally screened for resistance to thiophanate-methyl. The overall frequency of resistance to azoxystrobin and thiophanate-methyl was 48.0 % and 67.0 %, respectively. G143A in the cytochrome b gene (cyt b) was found in all C. nymphaeae and C. siamense isolates with high level of resistance, with EC50 > 100 µg/ml, while F129L was found in two of the five C. nymphaeae isolates with moderate resistance, with EC50 values ranging from 2.6 to 7.8 µg/ml. All C. fioriniae isolates tested were found to be less sensitive to azoxystrobin, with EC50 values ranging from 9.7 to 14.4 µg/ml, despite no mutations detected in cyt b. Moreover, E198A in tub2 was linked with C. siamense isolates resistant to thiophanate-methyl (EC50 > 100 µg/ml). These results revealed that resistance in Colletotrichum spp. to primary fungicides was widespread in the Mid-Atlantic strawberry fields.

scholarly journals Isolation and Characterization of Two Rare Mucoralean Species with Specific Habitats

2018 ◽  
Author(s):  
Seo Hee Lee ◽  
Thuong T. T. Nguyen ◽  
Hyang Burm Lee
Keyword(s):  
Internal Transcribed Spacer ◽  
Fungal Diversity ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Sequence Analyses ◽  
Saprotrophic Fungi ◽  
Isolation And Characterization ◽  
Gilbertella Persicaria ◽  
28S Ribosomal Dna

The order Mucorales, the largest in number of species within the Mucoromycotina, comprises typically fast-growing saprotrophic fungi. During a study of the fungal diversity of undiscovered taxa in Korea, two novel mucoralean strains, CNUFC-GWD3-9 and CNUFC-EGF1-4, were isolated from specific habitats including freshwater and fecal samples, respectively, in Korea. On the basis of their morphological characteristics and sequence analyses of internal transcribed spacer (ITS) and large subunit (LSU) of 28S ribosomal DNA regions, the CNUFC-GWD3-9 and CNUFC-EGF1-4 isolates were confirmed to be Gilbertella persicaria and Pilobolus crystallinus, respectively. It is ecologically, pathologically and mycologically significant to find such rare zygomycetous fungi in such specific habitats. 

scholarly journals Assessment of chilli genotypes for anthracnose resistance under field conditions

2020 ◽  
Vol 15 (2) ◽  
pp. 67-72
Author(s):  
K. Arjun ◽  
T. Arumugam ◽  
M. Karthikeyan ◽  
H. Usha Nandhini Devi ◽  
S. Mohankumar
Keyword(s):  
Disease Index ◽  
Field Conditions ◽  
High Yield ◽  
Anthracnose Resistance ◽  
Colletotrichum Species ◽  
Cent Disease ◽  
Moderately Resistant ◽  
Resistant Genotypes ◽  
Important Disease

Anthracnose caused by complex of Colletotrichum species is an economically important disease of chilli. The study involved 132 genotypes grown under field conditions. Among the genotypes evaluated, fifteen genotypes were moderately resistant to anthracnose with fairly high yield. In the order of merit, the genotypes were CA 177 (10.74%), Paramakudi 1 (16.64%), F 507 (17.17%), Bird’s eye chilli (17.85%), Kadaladi 1 (19.70%), Ramnad local (20.64%), CO 1 (20.82%), TA/CA/ 10 (21.56%), CA 166 (21.94%), CA 188 (22.29%), Paramakudi 2 (22.45%), CA 13/6 (23.42%), Chilli CO hybrid 1 (24.36%), IC 342465 (24.68%) and CA 165 (25.70%). whereas, 40 genotypes were susceptible with PDI ranging from 26.30 to 50.69 per cent and majority of the genotypes (77nos) were found to be highly susceptible to anthracnose with per cent disease index ranging from 51.81 to 96.42 per cent. The moderately resistant genotypes identified in the present investigation will serve as donors or source of resistance for anthracnose.

scholarly journals Molecular Characterization, Pathogenicity and Biological Characterization of Colletotrichum Species Associated with Anthracnose of Camellia yuhsienensis Hu in China

Forests ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1712
Author(s):  
Xinggang Chen ◽  
Lingyu Jiang ◽  
Anhua Bao ◽  
Changlin Liu ◽  
Junang Liu ◽  
...  
Keyword(s):  
Mycelial Growth ◽  
Morphological Characteristics ◽  
Biological Characteristics ◽  
Camellia Oleifera ◽  
Biological Characterization ◽  
Pathogenicity Tests ◽  
Colletotrichum Spp ◽  
And Control ◽  
First Time

Camellia yuhsienensis Hu, a species of tea oil tree with resistance to anthracnose, is widely used to breed disease-resistant Camellia varieties. In 2019, anthracnose symptoms were observed on Ca. yuhsienensis for the first time. However, the species and biological characteristics of Colletotrichum spp. isolated from Ca. yuhsienensis (YX-Colletotrichum spp.) have not been elucidated. In this study, five isolates (YX2-5-2, 2YX-3-1, 2YX-5-1, 2YX-8-1-1 and 2YX-8-1-2), which were consistent with the morphological characteristics of Colletotrichum spp., were obtained from Ca. yuhsienensis. A phylogenetic analysis demonstrated that YX2-5-2, 2YX-3-1 and 2YX-8-1-2 belonged to first clade along with Colletotrichum fructicola. 2YX-8-1-1 belonged to the second clade along with Colletotrichum siamense. 2YX-5-1 belonged to the third clade with Colletotrichum camelliae. Pathogenicity tests revealed that the pathogenicity of YX-Colletotrichum spp. was stronger than that of Colletotrichum spp. isolated from Camellia oleifera (GD-Colletotrichum spp.). Biological characteristics illustrated that the mycelial growth of YX-Co. camelliae (2YX-5-1) was slower than that of GD-Co. camelliae when the temperature exceeded 20 °C. In addition, in the presence of ions, the mycelial growth of YX-Co. fructicola (YX2-5-2) and YX-Co. siamense (2YX-8-1-1) was also slower than that of GD-Co. fructicola and GD-Co. siamense. Furthermore, the ability of YX-Colletotrichum spp. to utilize lactose and mannitol was weaker than that of GD-Colletotrichum spp., while the ability to utilize NH4+ was generally stronger than that of GD-Colletotrichum spp. This is the first report of anthracnose of Ca. yuhsienensis induced by Co. fructicola, Co. siamense and Co. camelliae in China. These results will provide theoretical guidance for the study of the pathogenesis and control of anthracnose on Ca. yuhsienensis.

scholarly journals Characterization of Two Undescribed Mucoralean Species with Specific Habitats in Korea

2018 ◽  
Author(s):  
Seo Hee Lee ◽  
Thuong T. T. Nguyen ◽  
Hyang Burm Lee
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Fungal Diversity ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Sequence Analyses ◽  
Saprotrophic Fungi ◽  
Gilbertella Persicaria ◽  
28S Ribosomal Dna

The order Mucorales, the largest in number of species within the Mucoromycotina, comprises typically fast-growing saprotrophic fungi. During a study of the fungal diversity of undiscovered taxa in Korea, two mucoralean strains, CNUFC-GWD3-9 and CNUFC-EGF1-4, were isolated from specific habitats including freshwater and fecal samples, respectively, in Korea. The strains were analyzed both for morphology and phylogeny based on the internal transcribed spacer (ITS) and large subunit (LSU) of 28S ribosomal DNA regions. On the basis of their morphological characteristics and sequence analyses, isolates CNUFC-GWD3-9 and CNUFC-EGF1-4 were confirmed to be Gilbertella persicaria and Pilobolus crystallinus, respectively.To the best of our knowledge, there are no published literature records of these two genera in Korea.

Sign in / Sign up

Export Citation Format

Share Document