scholarly journals Ribotypes of Polymyxa graminis in Wheat Samples Infected with Soilborne Wheat Viruses in China

Plant Disease ◽  
2018 ◽  
Vol 102 (5) ◽  
pp. 948-954 ◽  
Author(s):  
Yu Xu ◽  
Lifeng Hu ◽  
Linying Li ◽  
Yan Zhang ◽  
Bingjian Sun ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Mosaic Virus ◽  
Wheat Root ◽  
Plant Viruses ◽  
Yellow Mosaic Virus ◽  
Wheat Yellow Mosaic Virus ◽  
Polymyxa Graminis ◽  
Obvious Association ◽  
Soilborne Viruses ◽  
Chinese Wheat

Polymyxa graminis is an obligate parasite and important vector of more than 14 soilborne plant viruses that pose a significant threat to cereal crops in Europe, North America, and Asia. Different ribotypes or formae speciales of P. graminis have been recognized and these may be associated with different cereal hosts or with transmission of different viruses. Two soilborne viruses infecting winter wheat in China have been reported and well studied (Wheat yellow mosaic virus [WYMV, genus Bymovirus] and Chinese wheat mosaic virus [CWMV, genus Furovirus]) but there has been no reported characterization of P. graminis isolates associated with them. In this study, the ribosomal DNA internal transcribed spacer (ITS) regions of P. graminis were examined from 63 wheat samples with apparent virus symptoms obtained from 16 sites within six Chinese provinces. Their associations with soilborne viruses were investigated. Ribotype I (P. graminis f. sp. temperata) and ribotype II (P. graminis f. sp. tepida) were confirmed in winter wheat regions of China for the first time. All 63 wheat root samples were infected with ribotype I of P. graminis and 11 were also infected with ribotype II. There was no obvious association between the ribotypes and infection by either WYMV or CWMV (or double infection). Phylogenetic analysis of the P. graminis ITS1-5.8S-ITS2 sequences revealed that ribotype I in China belongs to previously reported subgroup Ib, whereas ribotype II belongs to IIa. There was considerable sequence variation (pairwise distances from 0.0219 to 0.0319) between Chinese ribotype I isolates of different regions and previously reported ribotype I isolate Ken5 (accession number HE860055.1).

Polymyxa graminis. [Distribution map].

2011 ◽  
Author(s):  
Keyword(s):  
New York ◽  
South Carolina ◽  
Mosaic Virus ◽  
Prince Edward Island ◽  
Plant Viruses ◽  
Yellow Mosaic Virus ◽  
Distribution Map ◽  
Wheat Yellow Mosaic Virus ◽  
Polymyxa Graminis ◽  
Distribution In Europe

Abstract A new distribution map is provided for Polymyxa graminis Ledingham. Cercozoa: Plasmodiophorida. Hosts: groundnut (Arachis hypogaea), oats (Avena sativa), barley (Hordeum vulgare), rice (Oryza sativa), rye (Secale cereale), wheat (Triticum aestivum), millet (Panicum miliaceum) and sorghum (Sorghum bicolor). Information is given on the geographical distribution in Europe (Belgium, France, Mainland France, Germany, Greece, Mainland Greece, Italy, Mainland Italy, Netherlands, Spain, Mainland Spain, UK, England and Wales), Asia (China, Anhui, Gansu, Henan, Hubei, Hunan, Jiangsu, Jiangxi, Shaanxi, Shandong, Shanxi, Sichuan, Zhejiang, India, Andhra Pradesh, Gujarat, Punjab, Rajasthan, Iran, Japan, Honshu, Lebanon, Pakistan, Syria, Turkey), Africa (Burkina Faso, Cote d'Ivoire, Mali, Niger, Senegal), North America (Canada, Manitoba, Ontario, Prince Edward Island, USA, Arkansas, Florida, Georgia, Illinois, Iowa, Kansas, Kentucky, Maryland, Missouri, Nebraska, New Mexico, New York, Oklahoma, Pennsylvania, South Carolina, Virginia, Washington), South America (Bolivia, Brazil, Rio Grande do Sul, Colombia), Oceania (New Zealand). Vector of numerous plant viruses, including Soil-borne wheat mosaic virus, Wheat yellow mosaic virus, Wheat spindle streak mosaic virus, Barley yellow mosaic virus, Oat mosaic virus, Rice stripe necrosis virus and Peanut clump virus. Several viruses transmitted by P. graminis cause major widespread diseases on barley, wheat and groundnut. P. graminis is presumed to be present in all fields infested with the viral diseases it transmits, but is also present in virus-free soils. The distribution of the viruses transmitted by P. graminis is greater than the confirmed distribution of P. graminis shown on the map.

scholarly journals Genome-Wide Identification and Expression Analysis of the Histone Deacetylase Gene Family in Wheat (Triticum aestivum L.)

Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 19
Author(s):  
Peng Jin ◽  
Shiqi Gao ◽  
Long He ◽  
Miaoze Xu ◽  
Tianye Zhang ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Gene Family ◽  
Mosaic Virus ◽  
Stress Responses ◽  
Viral Infections ◽  
Phylogenetic Analyses ◽  
Plant Viruses ◽  
Gene Family Evolution ◽  
Yellow Mosaic Virus ◽  
Wheat Yellow Mosaic Virus

Histone acetylation is a dynamic modification process co-regulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs). Although HDACs play vital roles in abiotic or biotic stress responses, their members in Triticumaestivum and their response to plant viruses remain unknown. Here, we identified and characterized 49 T. aestivumHDACs (TaHDACs) at the whole-genome level. Based on phylogenetic analyses, TaHDACs could be divided into 5 clades, and their protein spatial structure was integral and conserved. Chromosomal location and synteny analyses showed that TaHDACs were widely distributed on wheat chromosomes, and gene duplication has accelerated the TaHDAC gene family evolution. The cis-acting element analysis indicated that TaHDACs were involved in hormone response, light response, abiotic stress, growth, and development. Heatmaps analysis of RNA-sequencing data showed that TaHDAC genes were involved in biotic or abiotic stress response. Selected TaHDACs were differentially expressed in diverse tissues or under varying temperature conditions. All selected TaHDACs were significantly upregulated following infection with the barley stripe mosaic virus (BSMV), Chinese wheat mosaic virus (CWMV), and wheat yellow mosaic virus (WYMV), suggesting their involvement in response to viral infections. Furthermore, TaSRT1-silenced contributed to increasing wheat resistance against CWMV infection. In summary, these findings could help deepen the understanding of the structure and characteristics of the HDAC gene family in wheat and lay the foundation for exploring the function of TaHDACs in plants resistant to viral infections.

scholarly journals Identification of a Novel Quinvirus in the Family Betaflexiviridae That Infects Winter Wheat

2021 ◽  
Vol 12 ◽  
Author(s):  
Hideki Kondo ◽  
Naoto Yoshida ◽  
Miki Fujita ◽  
Kazuyuki Maruyama ◽  
Kiwamu Hyodo ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Mosaic Virus ◽  
De Novo ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Local Alignment ◽  
Limited Information ◽  
Wheat Yellow Mosaic Virus ◽  
Yellow Mosaic Disease ◽  
The Impact

Yellow mosaic disease in winter wheat is usually attributed to the infection by bymoviruses or furoviruses; however, there is still limited information on whether other viral agents are also associated with this disease. To investigate the wheat viromes associated with yellow mosaic disease, we carried out de novo RNA sequencing (RNA-seq) analyses of symptomatic and asymptomatic wheat-leaf samples obtained from a field in Hokkaido, Japan, in 2018 and 2019. The analyses revealed the infection by a novel betaflexivirus, which tentatively named wheat virus Q (WVQ), together with wheat yellow mosaic virus (WYMV, a bymovirus) and northern cereal mosaic virus (a cytorhabdovirus). Basic local alignment search tool (BLAST) analyses showed that the WVQ strains (of which there are at least three) were related to the members of the genus Foveavirus in the subfamily Quinvirinae (family Betaflexiviridae). In the phylogenetic tree, they form a clade distant from that of the foveaviruses, suggesting that WVQ is a member of a novel genus in the Quinvirinae. Laboratory tests confirmed that WVQ, like WYMV, is potentially transmitted through the soil to wheat plants. WVQ was also found to infect rye plants grown in the same field. Moreover, WVQ-derived small interfering RNAs accumulated in the infected wheat plants, indicating that WVQ infection induces antiviral RNA silencing responses. Given its common coexistence with WYMV, the impact of WVQ infection on yellow mosaic disease in the field warrants detailed investigation.

scholarly journals Efficient CRISPR/Cas-Mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1481
Author(s):  
Florian Hahn ◽  
Laura Sanjurjo Sanjurjo Loures ◽  
Caroline A. Sparks ◽  
Kostya Kanyuka ◽  
Vladimir Nekrasov
Keyword(s):  
Winter Wheat ◽  
Mosaic Virus ◽  
Genome Editing ◽  
Plant Viruses ◽  
Targeted Mutagenesis ◽  
Experimental Methodology ◽  
Yellow Mosaic Virus ◽  
Wheat Varieties ◽  
Mutant Lines ◽  
Winter Wheat Varieties

CRISPR/Cas technology has recently become the molecular tool of choice for gene function studies in plants as well as crop improvement. Wheat is a globally important staple crop with a well annotated genome and there is plenty of scope for improving its agriculturally important traits using genome editing technologies, such as CRISPR/Cas. As part of this study we targeted three different genes in hexaploid wheat Triticum aestivum: TaBAK1-2 in the spring cultivar Cadenza as well as Ta-eIF4E and Ta-eIF(iso)4E in winter cultivars Cezanne, Goncourt and Prevert. Primary transgenic lines carrying CRISPR/Cas-induced indels were successfully generated for all targeted genes. While BAK1 is an important regulator of plant immunity and development, Ta-eIF4E and Ta-eIF(iso)4E act as susceptibility (S) factors required for plant viruses from the Potyviridae family to complete their life cycle. We anticipate the resultant homozygous tabak1-2 mutant lines will facilitate studies on the involvement of BAK1 in immune responses in wheat, while ta-eif4e and ta-eif(iso)4e mutant lines have the potential to become a source of resistance to wheat spindle streak mosaic virus (WSSMV) and wheat yellow mosaic virus (WYMV), both of which are important pathogens of wheat. As winter wheat varieties are generally less amenable to genetic transformation, the successful experimental methodology for transformation and genome editing in winter wheat presented in this study will be of interest to the research community working with this crop.

scholarly journals Towards plant resistance to viruses using protein-only RNase P

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Anthony Gobert ◽  
Yifat Quan ◽  
Mathilde Arrivé ◽  
Florent Waltz ◽  
Nathalie Da Silva ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Virus Replication ◽  
Yield Loss ◽  
Rnase P ◽  
Plant Viruses ◽  
Yellow Mosaic Virus ◽  
Resistance To Viruses ◽  
Target Plant

AbstractPlant viruses cause massive crop yield loss worldwide. Most plant viruses are RNA viruses, many of which contain a functional tRNA-like structure. RNase P has the enzymatic activity to catalyze the 5′ maturation of precursor tRNAs. It is also able to cleave tRNA-like structures. However, RNase P enzymes only accumulate in the nucleus, mitochondria, and chloroplasts rather than cytosol where virus replication takes place. Here, we report a biotechnology strategy based on the re-localization of plant protein-only RNase P to the cytosol (CytoRP) to target plant viruses tRNA-like structures and thus hamper virus replication. We demonstrate the cytosol localization of protein-only RNase P in Arabidopsis protoplasts. In addition, we provide in vitro evidences for CytoRP to cleave turnip yellow mosaic virus and oilseed rape mosaic virus. However, we observe varied in vivo results. The possible reasons have been discussed. Overall, the results provided here show the potential of using CytoRP for combating some plant viral diseases.

Sign in / Sign up

Export Citation Format

Share Document