scholarly journals Developmental Dynamics of Globodera ellingtonae in Field-Grown Potato

Plant Disease ◽  
2017 ◽  
Vol 101 (7) ◽  
pp. 1182-1187 ◽  
Author(s):  
Wendy S. Phillips ◽  
Megan Kitner ◽  
Inga A. Zasada
Keyword(s):  
Developmental State ◽  
Bare Soil ◽  
Potato Cyst Nematodes ◽  
Life Stages ◽  
Cyst Nematodes ◽  
Close Relationship ◽  
Annual Reduction ◽  
Developmental Dynamics ◽  
Two Peaks ◽  
Relationship Of

Globodera ellingtonae is a recently described nematode parasite of potato, which is closely related to the economically significant potato cyst nematodes G. rostochiensis and G. pallida. Because of the close relationship of G. ellingtonae to the potato cyst nematodes, a greater understanding of its biology is critical. Two experiments were conducted in Oregon to explore the developmental biology of G. ellingtonae in field-grown potato. The first experiment was conducted in 2013 and 2014 to determine the developmental timing of G. ellingtonae life stages and reproduction by inoculating potato with soil containing cysts followed by weekly collection of soil and root samples. Life stages; second-stage juveniles (J2) in soil and roots, third-stage juveniles (J3) and fourth-stage (J4) females and males in roots, males and females or cysts in soil, and egg number and developmental state were quantified. Normalizing across years using accumulated developmental degree days above 6°C (DD6), J2 of G. ellingtonae were found in soil from 41 to 588 DD6; two peaks of J2 invasion of roots were observed. The first adult females were observed at 387 and 449 DD6 in 2013 and 2014, respectively. The next generation of eggs was first observed from 675 to 854 DD6 and 50% egg development (containing a vermiform juvenile) occurred at approximately 920 DD6. A second J2 hatch was observed in both years at 927 to 1,073 DD6. The developmental dynamics of G. ellingtonae observed here are similar to those reported for G. rostochiensis and G. pallida from several geographical locations. In the second experiment, the effect of potato and bare soil on G. ellingtonae egg hatch was evaluated; in 2014 and 2015, packages containing cysts in soil were buried under potato or in bare soil at the time of planting and eggs per cyst determined weekly. Across years, a significant reduction in eggs per cysts under potato (>50%) was observed 35 days after planting (DAP) and, at 63 DAP, eggs per cyst were reduced by 76 to 96% compared with initial egg per cyst densities. In bare soil, the maximum reduction in densities of eggs per cyst was 55 to 73%. This annual reduction in egg numbers of G. ellingtonae in bare soil is similar to that reported for G. pallida and G. rostochiensis.

Effects of Various Sewage Sludge Treatment Processes on the Survival of Potato Cyst-Nematodes (Globodera spp.) and the Implications for Disposal

1989 ◽  
Vol 21 (8-9) ◽  
pp. 909-916
Author(s):  
A. M. Spaull ◽  
D. M. McCormack ◽  
E. B. Pike
Keyword(s):  
Anaerobic Digestion ◽  
Potato Cyst Nematodes ◽  
Cyst Nematodes ◽  
Sludge Treatment ◽  
Treatment Processes ◽  
Sewage Sludge Treatment ◽  
Thermophilic Digestion ◽  
C 30 ◽  
Effluent Exposure ◽  
Potato Crops

Samples of sewage sludges, taken over a 12-month period from 9 Scottish sewage works, contained on average 0.24 cysts of Globodera spp. (potato cyst-nematodes) of which 11% were viable. The incidence was not significantly related to season or to the presence of vegetable-processing effluent. Exposure of cysts in sludge to mesophilic anaerobic digestion (35 °C, 30 min) cold anaerobic digestion (9 weeks), pasteurisation (70 °C, 30 min) and aerobic thermophilic digestion (60 °C, ld) reduced viability of eggs within the cysts by almost 100%. Sludges so treated can therefore be considered to be free from infection risk to potato crops, although the non-infective cysts may still be recovered. Treatment with lime at pH 11.5 (20 °C, 24 h), by aerobic stabilisation in an oxidation ditch (7 weeks) and by activated-sludge treatment (5d) did not reduced viability acceptably. Accelerated cold digestion did not reduce viability sufficiently after the usual 15 weeks but rendered eggs completely non-viable after 21 weeks. The results show that even sludge treated to destroy viable cysts should not be applied to land used for growing seed potatoes and subject to testing for freedom from infestation. Treatment destroying viability should increase the acceptability of sludge for ware potato growers, although the numbers of cysts applied in untreated sludge would be unlikely to increase significantly levels of cysts in soils already infested.

scholarly journals Taxonomy, Morphological and Molecular Identification of the Potato Cyst Nematodes, Globodera pallida and G. rostochiensis

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 184
Author(s):  
John Wainer ◽  
Quang Dinh
Keyword(s):  
Life Cycle ◽  
Molecular Identification ◽  
Soil Sampling ◽  
Globodera Pallida ◽  
Potato Cyst Nematodes ◽  
Cyst Nematodes ◽  
Primary Focus

The scope of this paper is limited to the taxonomy, detection, and reliable morphological and molecular identification of the potato cyst nematodes (PCN) Globodera pallida and G. rostochiensis. It describes the nomenclature, hosts, life cycle, pathotypes, and symptoms of the two species. It also provides detailed instructions for soil sampling and extraction of cysts from soil. The primary focus of the paper is the presentation of accurate and effective methods to identify the two principal PCN species.

NOTES ON THE GENUS PROTARCHOIDES (HYMEN., ICHNEUMONIDAE)

1938 ◽  
Vol 70 (11) ◽  
pp. 230-232 ◽  
Author(s):  
C. Stuart Walley
Keyword(s):  
North American ◽  
North American Species ◽  
Close Relationship ◽  
Relationship Of

The following notes were assembled in arranging the Protarchoides material in the National Collection. In establishing the identity of Protarchoides mellipes (Prov.) it has been found necessary to synonymize one species. A species allied to mellipes is described as new and a table is provided for the separation of the four known North American species. The recording of Trichiosoma as host for a member of this genus is further evidence of the close relationship of the genus with Protarchus Foer.

scholarly journals Study of the 49 kDa excretory-secretory protein gene of Trichinella nativa and Trichinella spiralis

2007 ◽  
Vol 44 (3) ◽  
pp. 120-125 ◽  
Author(s):  
B. Zheng ◽  
L. Xiao ◽  
X. Wang ◽  
D. Li ◽  
Y. Lu ◽  
...  
Keyword(s):  
Prokaryotic Expression ◽  
Protein Gene ◽  
Trichinella Spiralis ◽  
Secretory Protein ◽  
Rt Pcr ◽  
Prokaryotic Expression Vector ◽  
Close Relationship ◽  
Bamhi Site ◽  
Relationship Of ◽  
Trichinella Nativa

AbstractTo study the function of the 49 kDa excretory-secretory (ES) protein gene (P49) of Trichinella, the genes was amplified by RT-PCR from RNA of Trichinella spiralis and Trichinella nativa and several Chinese Trichinella isolates of domestic animals, and sequenced after being cloned. The amplified products of these parasites produced bands of about 950 bp. The 97.2 % to 100 % nucleotides identity and 94.3 % to 100 % identity of deduced amino acids among P49 gene of these Trichinella strains showed the close relationship of these parasites. The P49 gene of T. nativa was cloned into the BamHI site of the prokaryotic expression vector pET-30a, and the recombinant vector was expressed. The expressed product was 40.8 kDa in size. In Western blot analysis, the expressed product was reactive to sera of mice infected with T. nativa, T. spiralis and their Chinese geographical strains.

The occurrence and distribution of potato cyst nematodes in Croatia

2006 ◽  
Vol 80 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Dinka Grubišić ◽  
Ljerka Oštrec ◽  
Tanja Gotlin Čuljak ◽  
Sylvia Blümel
Keyword(s):  
Potato Cyst Nematodes ◽  
Cyst Nematodes
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