scholarly journals Molecular and Biological Characterization of Tomato mottle mosaic virus and Development of RT-PCR Detection

Plant Disease ◽  
2017 ◽  
Vol 101 (5) ◽  
pp. 704-711 ◽  
Author(s):  
Xuelian Sui ◽  
Yi Zheng ◽  
Rugang Li ◽  
Chellappan Padmanabhan ◽  
Tongyan Tian ◽  
...  
Keyword(s):  
New York ◽  
South Carolina ◽  
Mosaic Virus ◽  
Genetic Relationships ◽  
The United States ◽  
Biological Properties ◽  
Tomato Mosaic Virus ◽  
Rt Pcr ◽  
The World ◽  
Species Specific

Tomato mottle mosaic virus (ToMMV) was first identified in 2013 as a novel tobamovirus infecting tomatoes in Mexico. In just a few years, ToMMV has been identified in several countries around the world, including the United States. In the present study, we characterized the molecular, serological, and biological properties of ToMMV and developed a species-specific RT-PCR to detect three tomato-infecting tobamoviruses: Tobacco mosaic virus (TMV), Tomato mosaic virus (ToMV), and ToMMV. Previously, ToMMV has been reported in Florida and New York. In this study, we made two new reports on the occurrences of ToMMV on tomatoes in California and South Carolina. Their complete genome sequences were obtained and their genetic relationships to other tobamoviruses evaluated. In host range studies, some differential responses in host plants were also identified between ToMMV and ToMV. To alleviate cross-serological reactivity among the tomato-infecting tobamoviruses, a new multiplex RT-PCR was developed to allow for species-specific detection and identification of TMV, ToMV, and ToMMV. In addition, we observed resistance breaking by ToMMV on selected tomato cultivars that were resistant to ToMV. This has caused serious concerns to tomato growers worldwide. In conclusion, the characterization in molecular and biological properties of ToMMV would provide us with fundamental knowledge to manage this emerging virus on tomato and other solanaceous crops in the U.S. and around the world.

scholarly journals Differential Detection of the Tobamoviruses Tomato Mosaic Virus (ToMV) and Tomato Brown Rugose Fruit Virus (ToBRFV) Using CRISPR-Cas12a

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1256
Author(s):  
Dan Mark Alon ◽  
Hagit Hak ◽  
Menachem Bornstein ◽  
Gur Pines ◽  
Ziv Spiegelman
Keyword(s):  
Mosaic Virus ◽  
Tomato Mosaic Virus ◽  
Differential Detection ◽  
Rt Pcr ◽  
Specific Detection ◽  
Novel Approach ◽  
Pcr Product ◽  
Species Specific ◽  
User Friendly ◽  
Efficient Sequence

CRISPR/Cas12a-based detection is a novel approach for the efficient, sequence-specific identification of viruses. Here we adopt the use of CRISPR/Cas12a to identify the tomato brown rugose fruit virus (ToBRFV), a new and emerging tobamovirus which is causing substantial damage to the global tomato industry. Specific CRISPR RNAs (crRNAs) were designed to detect either ToBRFV or the closely related tomato mosaic virus (ToMV). This technology enabled the differential detection of ToBRFV and ToMV. Sensitivity assays revealed that viruses can be detected from 15–30 ng of RT-PCR product, and that specific detection could be achieved from a mix of ToMV and ToBRFV. In addition, we show that this method can enable the identification of ToBRFV in samples collected from commercial greenhouses. These results demonstrate a new method for species-specific detection of tobamoviruses. A future combination of this approach with isothermal amplification could provide a platform for efficient and user-friendly ways to distinguish between closely related strains and resistance-breaking pathogens.

Bringing the Real World of Science to Children: A Partnership of the American Museum of Natural History and the City University of New York

2008 ◽  
Vol 12 (1) ◽  
Author(s):  
Anthony G Picciano ◽  
Robert V. Steiner
Keyword(s):  
United States ◽  
New York ◽  
Natural History ◽  
Local Governments ◽  
Compulsory Education ◽  
Development Program ◽  
The United States ◽  
American Museum ◽  
The World ◽  
The City

Every child has a right to an education. In the United States, the issue is not necessarily about access to a school but access to a quality education. With strict compulsory education laws, more than 50 million students enrolled in primary and secondary schools, and billions of dollars spent annually on public and private education, American children surely have access to buildings and classrooms. However, because of a complex and competitive system of shared policymaking among national, state, and local governments, not all schools are created equal nor are equal education opportunities available for the poor, minorities, and underprivileged. One manifestation of this inequity is the lack of qualified teachers in many urban and rural schools to teach certain subjects such as science, mathematics, and technology. The purpose of this article is to describe a partnership model between two major institutions (The American Museum of Natural History and The City University of New York) and the program designed to improve the way teachers are trained and children are taught and introduced to the world of science. These two institutions have partnered on various projects over the years to expand educational opportunity especially in the teaching of science. One of the more successful projects is Seminars on Science (SoS), an online teacher education and professional development program, that connects teachers across the United States and around the world to cutting-edge research and provides them with powerful classroom resources. This article provides the institutional perspectives, the challenges and the strategies that fostered this partnership.

“Little Plants in the Country”: Henry Ford's Village Industries and the Beginning of Decentralized Technology in Modern America

Prospects ◽  
1988 ◽  
Vol 13 ◽  
pp. 181-223 ◽  
Author(s):  
Howard P. Segal
Keyword(s):  
United States ◽  
Decision Making ◽  
New York ◽  
Information Processing ◽  
Real Estate ◽  
New York Times ◽  
The United States ◽  
Physical Proximity ◽  
The World ◽  
Word Processors

“Technology Spurs Decentralization Across the Country.” So reads a 1984 New York Times article on real-estate trends in the United States. The contemporary revolution in information processing and transmittal now allows large businesses and other institutions to disperse their offices and other facilities across the country, even across the world, without loss of the policy- and decision-making abilities formerly requiring regular physical proximity. Thanks to computers, word processors, and the like, decentralization has become a fact of life in America and other highly technological societies.

THE TEN-STATE NUTRITION SURVEY: A PEDIATRIC PERSPECTIVE

PEDIATRICS ◽  
1973 ◽  
Vol 51 (6) ◽  
pp. 1095-1099
Author(s):  
Charles U. Lowe ◽  
Gilbert B. Forbes ◽  
Stanley Garn ◽  
George M. Owen ◽  
Nathan J. Smith ◽  
...  
Keyword(s):  
United States ◽  
New York ◽  
South Carolina ◽  
Selection Process ◽  
The United States ◽  
Food Utilization ◽  
Nutrition Survey ◽  
Dietary Recall ◽  
Department Of Health ◽  
The Individual

In 1967 the 90th Congress of the United States attached an amendment to the Partnership for Health Act requiring the Secretary of the Department of Health, Education, and Welfare to undertake a survey of "the incidence and location of serious hunger and malnutrition–in the United States." In response to the legislative mandate the Ten-State Nutrition Survey was conducted during the years 1968 through 1970. The sample was selected from urban and rural families living in the following ten states: New York, Massachusetts, Michigan, California, Washington, Kentucky, West Virginia, Louisiana, Texas, and South Carolina. The families selected were those living in some of the census enumeration districts that made up the lowest economic quartiles of their respective states at the time of the 1960 census. During the eight years after the 1960 census the social and economic characteristics found in some of the individual enumeration districts had changed, so that there was a significant numer of families in the surveys with incomes well above the lowest income quartile. Thus, it was possible in analyzing results to make some comparisons on an economic basis. Thirty thousand families were identified in the selection process; 23,846 of these participated in the survey. Data regarding more than 80,000 individuals were obtained through interviews and 40,847 of these individuals were examined. The survey included the following: extensive demographic information on each of the participating families; information regarding food utilization of the family; a 24-hour dietary recall for infants up to 36 months of age, children 10 to 16 years of age, pregnant and lactating women, and individuals over 60 years of age.

Maize dwarf mosaic virus. [Distribution map].

2003 ◽  
Author(s):  
Keyword(s):  
New York ◽  
South Carolina ◽  
Mosaic Virus ◽  
Uttar Pradesh ◽  
Saccharum Officinarum ◽  
Maize Dwarf Mosaic Virus ◽  
Distribution Map ◽  
Central Russia ◽  
Virus Distribution ◽  
Distribution In Europe

Abstract A new distribution map is provided for Maize dwarf mosaic virus Viruses: Potyviridae: Potyvirus Hosts: Maize (Zea mays), sorghum (Sorghum bicolor), also sugarcane (Saccharum officinarum), millet (Panicum miliaceum) and many other Poaceae. Information is given on the geographical distribution in EUROPE, Bosnia-Herzegovina, Bulgaria, Croatia, Czech Republic, France, Germany, Greece, Hungary, Italy, Romania, Central Russia Russia, Spain, Ukraine, Yugoslavia (Fed. Rep.), ASIA, China, Gansu, Hebei, Hubei, Jiangsu, Liaoning, Shaanxi, Shandong, Shanxi, India, Maharashtra, Uttar Pradesh, Iran, Iraq, Israel, Kazakhstan, Korea Republic, Pakistan, Taiwan, Turkey, Uzbekistan, Yemen, AFRICA, Burkina Faso, Cameroon, Cote d'Ivoire, Egypt, Ethiopia, Mauritius, Morocco, South Africa, Zambia, Zimbabwe, NORTH AMERICA, Canada, Ontario, Mexico, USA, Alabama, Arkansas, California, Delaware, Florida, Georgia, Hawaii, Idaho, Illinois, Indiana, Iowa, Kansas, Kentucky, Louisiana, Maine, Massachusetts, Michigan, Minnesota, Mississippi, Missouri, Nebraska, New Hampshire, New Jersey, New Mexico, New York, North Carolina, North Dakota, Ohio, Oklahoma, Pennsylvania, South Carolina, South Dakota, Tennessee, Texas, Vermont, Virginia, Washington, Wisconsin, CENTRAL AMERICA & CARIBBEAN, Cuba, Haiti, Honduras, SOUTH AMERICA Argentina, Brazil, Goias, Minas Gerais, Sao Paulo, Chile, Colombia, Peru, Venezuela, OCEANIA, Australia, Queensland, Victoria.

scholarly journals First Report of Potato spindle tuber viroid Naturally Infecting Field Tomatoes in the Dominican Republic

Plant Disease ◽  
2014 ◽  
Vol 98 (5) ◽  
pp. 701-701
Author(s):  
K.-S. Ling ◽  
R. Li ◽  
D. Groth-Helms ◽  
F. M. Assis-Filho
Keyword(s):  
United States ◽  
Dominican Republic ◽  
Mosaic Virus ◽  
Potato Spindle Tuber Viroid ◽  
The United States ◽  
Disease Outbreak ◽  
Ringspot Virus ◽  
Rt Pcr ◽  
Spot Virus ◽  
Three Samples

In recent years, viroid disease outbreaks have resulted in serious economic losses to a number of tomato growers in North America (1,2,3). At least three pospiviroids have been identified as the causal agents of tomato disease, including Potato spindle tuber viroid (PSTVd), Tomato chlorotic dwarf viroid (TCDVd), and Mexican papita viroid (MPVd). In the spring of 2013, a severe disease outbreak with virus-like symptoms (chlorosis and plant stunting) was observed in a tomato field located in the Dominican Republic, whose tomato production is generally exported to the United States in the winter months. The transplants were produced in house. The disease has reached an epidemic level with many diseased plants pulled and disposed of accordingly. Three samples collected in May of 2013 were screened by ELISA against 16 common tomato viruses (Alfalfa mosaic virus, Cucumber mosaic virus, Impatiens necrotic spot virus, Pepino mosaic virus, Potato virus X, Potato virus Y, Tobacco etch virus, Tobacco mosaic virus, Tobacco ringspot virus, Tomato aspermy virus, Tomato bushy stunt virus, Tomato mosaic virus, Tomato ringspot virus, Tomato spotted wilt virus, Groundnut ringspot virus, and Tomato chlorotic spot virus), a virus group (Potyvirus group), three bacteria (Clavibacter michiganensis subsp. michiganensis, Pectobacterium atrosepticum, and Xanthomonas spp.), and Phytophthora spp. No positive result was observed, despite the presence of symptoms typical of a viral-like disease. Further analysis by RT-PCR using Agdia's proprietary pospiviroid group-specific primer resulted in positive reactions in all three samples. To determine which species of pospiviroid was present in these tomato samples, full-genomic products of the expected size (~360 bp) were amplified by RT-PCR using specific primers for PSTVd (4) and cloned using TOPO-TA cloning kit (Invitrogen, CA). A total of 8 to 10 clones from each isolate were selected for sequencing. Sequences from each clone were nearly identical and the predominant sequence DR13-01 was deposited in GenBank (Accession No. KF683200). BLASTn searches into the NCBI database demonstrated that isolate DR13-01 shared 97% sequence identity to PSTVd isolates identified in wild Solanum (U51895), cape gooseberry (EU862231), or pepper (AY532803), and 96% identity to the tomato-infecting PSTVd isolate from the United States (JX280944). The relatively lower genome sequence identity (96%) to the tomato-infecting PSTVd isolate in the United States (JX280944) suggests that PSTVd from the Dominican Republic was likely introduced from a different source, although the exact source that resulted in the current disease outbreak remains unknown. It may be the result of an inadvertent introduction of contaminated tomato seed lots or simply from local wild plants. Further investigation is necessary to determine the likely source and route of introduction of PSTVd identified in the current epidemic. Thus, proper control measures could be recommended for disease management. The detection of this viroid disease outbreak in the Dominican Republic represents further geographic expansion of the viroid disease in tomatoes beyond North America. References: (1). K.-S. Ling and M. Bledsoe. Plant Dis. 93:839, 2009. (2) K.-S. Ling and W. Zhang. Plant Dis. 93:1216, 2009. (3) K.-S. Ling et al. Plant Dis. 93:1075, 2009. (4) A. M. Shamloul et al. Can. J. Plant Pathol. 19:89, 1997.

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