scholarly journals First Report of Phytophthora capsici as Causal Agent of Snap-bean (Phaseolus vulgaris) Pod Decay in Brazil

Plant Disease ◽  
2017 ◽  
Vol 101 (6) ◽  
pp. 1059 ◽  
Author(s):  
R. Petry ◽  
M. E. N. Fonseca ◽  
L. S. Boiteux ◽  
A. Reis
Keyword(s):  
Phaseolus Vulgaris ◽  
Phytophthora Capsici ◽  
Causal Agent ◽  
Snap Bean ◽  
First Report

scholarly journals First Report of Sida golden mosaic virus Infecting Snap Bean (Phaseolus vulgaris) in Florida

Plant Disease ◽  
2010 ◽  
Vol 94 (4) ◽  
pp. 487-487 ◽  
Author(s):  
T. C. Durham ◽  
C. Baker ◽  
L. Jones ◽  
L. Unruh Snyder
Keyword(s):  
Phaseolus Vulgaris ◽  
Mosaic Virus ◽  
Complete Sequence ◽  
Economic Implications ◽  
Snap Bean ◽  
First Report ◽  
Snap Beans ◽  
Alachua County ◽  
Pcr Products ◽  
Total Dna

In October 2006, snap bean (Phaseolus vulgaris ‘Titan’) plants in an Alachua County field exhibited symptoms of foliar mottling, puckering, and curl. Symptomatic plants were distributed along field margins infested with whiteflies (100% incidence). Six collected leaf specimens all tested positive for nuclear inclusion bodies typical of begomoviruses with the methodology outlined by Christie et al. (1). To confirm the putative begomovirus association, total DNA was extracted with Qiagen's DNeasy Plant Mini Kit (Qiagen, Valencia, CA). The degenerate Begomovirus primers 5′-GCCCACATYGTCTTYCCNGT-3′ and 5′-GGCTTYCTRTACATRGG-3′ were used to amplify a 1.1-kb fragment of DNA-A (2). Primers SiGMVf 5′-CCTAAGCGCGATTTGCCAT-3′ and SiGMVr 5′-TACAGGGAGCTAAATCCAGCT-3′ were designed to amplify the remaining 1.5 kb of the DNA-A component. The sequence of both PCR products was compiled to generate a complete sequence of an A component (2,633 nt). BLAST analysis of this sequence (GenBank Accession No. GQ357649) isolated from snap bean indicated 95% nucleotide identity to Sida golden mosaic virus (SiGMV) (GenBank Accession No. AF049336) isolated from Sida santaremensis from Florida. To our knowledge, this is the first report of SiGMV in Florida snap beans. Further study is warranted to examine the etiological and economic implications of this finding. References: (1) R. G. Christie et al. Phytopathology 76:124, 1986. (2) M. R. Rojas et al. Plant Dis. 77:340, 1993.

scholarly journals First Report of Phytophthora capsici Causing Foliar and Pod Blight of Snap Bean in China

Plant Disease ◽  
2018 ◽  
Vol 102 (2) ◽  
pp. 455-455
Author(s):  
J. F. Cao ◽  
C. Huo ◽  
M. Y. Yang ◽  
N. A. Rajput ◽  
Z. J. Zhao
Keyword(s):  
Phytophthora Capsici ◽  
Snap Bean ◽  
First Report

scholarly journals First Report of Blight of Common Bean Caused by Phytophthora capsici in Connecticut

Plant Disease ◽  
2010 ◽  
Vol 94 (1) ◽  
pp. 134-134 ◽  
Author(s):  
J. A. LaMondia ◽  
D. W. Li ◽  
C. R. Vossbrinck
Keyword(s):  
Phaseolus Vulgaris ◽  
New England ◽  
Phytophthora Capsici ◽  
Its Region ◽  
Weather Conditions ◽  
Flood Plain ◽  
Phaseolus Lunatus ◽  
Malt Extract ◽  
Connecticut River ◽  
First Report

Phytophthora capsici Leonion was first identified on pepper (Capsicum annuum L.) and is widespread on solanaceous and cucurbitaceous crops. It was first documented on Phaseolus lunatus L. in Delaware in 2002 (1), followed by reports on snap beans (Phaseolus vulgaris L.) in Michigan in 2003 (2), and on Long Island, NY in 2008 ( http://vegetablemdonline.ppath.cornell.edu/ NewsArticles/Bean_phytoJune09.html ). In 2009, we observed snap and wax beans in commercial production with water-soaked lesions on foliage, stems, and pods. Twelve to sixteen hectares were affected in the flood plain of the Connecticut River in central Connecticut. Weather conditions had been warm and very wet. Lesions displayed white mycelia and sporangia. P. capsici was isolated from surface-sterilized tissue on potato dextrose agar (PDA) and malt extract agar. Hyphal tips were subcultured onto V8 media for further analysis. To confirm Koch's postulates, two isolates were tested for pathogenicity against bean (cv. Valentino) and pepper (cv. Cayenne) by placing colonized PDA plugs or PDA alone next to the crown or in stem branches. Symptoms similar to those observed in the field on bean and pepper developed on inoculated plants and the pathogen was reisolated. Controls did not develop disease. Sporangia of P. capsici growing on V8 medium were ellipsoid, ovoid, pyriform, but occasionally irregular, papillate, and 54.0 ± 5.7 × 31.1 ± 4.7 μm (n = 31) with a length/width (L/W) ratio of 1.8 ± 0.3. The papillae were 5.4 ± 0.9 μm (n = 31) and the pedicels were 24.5 ± 12.6 × 3.0 ± 1.0 μm. Sporangia collected from bean plants were smaller with longer pedicels; the sporangia were 44.9 ± 9.1 × 26.0 ± 2.8 μm with a L/W ratio of 1.7 ± 0.2; papillae were 4.6 ± 1.0 μm; and the pedicels were 49 ± 20.0 × 2.8 ± 0.9 μm (n = 20). To confirm the identity of our isolate genetically, DNA was extracted from one P. capsici isolate and the nuclear ribosomal internal transcribed spacer (ITS) region was amplified and sequenced (GenBank Accession No. GU011684). The ITS sequence was identical to sequences of P. capsisci in GenBank and confirmed our identification of this new isolate as P. capsici. To our knowledge, this is the first report of P. capsici infecting Phaseolus vulgaris in Connecticut and New England. References: (1) C. R. Davidson et al. Plant Dis. 85:886, 2002. (2) A. J. Gevens et al. Plant Dis. 92:201, 2008.

scholarly journals First Report of Sida micrantha mosaic virus in Phaseolus vulgaris in Brazil

Plant Disease ◽  
2011 ◽  
Vol 95 (9) ◽  
pp. 1196-1196 ◽  
Author(s):  
N. A. N. Fernandes-Acioli ◽  
R. C. Pereira-Carvalho ◽  
R. S. Fontenele ◽  
C. Lacorte ◽  
S. G. Ribeiro ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Mosaic Virus ◽  
Universal Primers ◽  
Common Beans ◽  
Snap Bean ◽  
First Report ◽  
Solanaceae Species ◽  
Biotype B

Snap and common beans (Phaseolus vulgaris L.) are severely affected by Bean golden mosaic virus (BGMV) infection, so far the only begomovirus reported on these crops in Brazil (1). Samples of snap and common beans colonized by the whitefly Bemisia tabaci biotype B and displaying golden mosaic, chlorotic spots, and leaf distortion were collected in three production regions in Goiás State (Goianápolis, Luziânia, and Itaberaí) between 2003 and 2007. Total DNA extracted from leaf samples was used as template in PCR assays using universal primers targeting conserved regions of the DNA-A and DNA-B genomes (3). Begomovirus-specific amplicons were observed only with DNA template from symptomatic plants. Two single amplicons were observed for both genomic segments, indicating the presence of bipartite species in all samples. Sequence analysis of four isolates (named as GO-176, GO-260, GO-354, and GO-368) obtained from common bean samples indicated identity levels of approximately 95% with the DNA-A segment of BGMV (GenBank Accession No. FJ665283). However, the complete DNA-A sequence (GenBank Accession No. HM357459.1) of the GO-060 isolate (from a symptomatic snap bean plant collected in Goianápolis) displayed 76% identity with BGMV (GenBank Accession No. FJ665283) and 95% identity with the DNA-A of a Sida micrantha mosaic virus (SimMV) isolate (GenBank Accession No. EU908733.1) reported to be infecting okra (Abelmoschus esculentus L.) and 94.8% with a SimMV isolate reported to be infecting soybean (GenBank Accession No. FJ686693) in Brazil (2). Koch's postulates were fulfilled for the isolate GO-060 by inoculating a set of soybean and bean accessions via a biolistic approach. The ratio of positive PCR amplicons per total of inoculated plants were 15 of 16 for snap bean cv. Trepador, 9 of 10 for snap bean cv. Fartura, 18 of 24 for common bean cv. Olate Pinto, and 19 of 25 for common bean cv. Carioca. The isolate was also able to infect eight of nine soybean ‘Doko’ plants. Sequence analysis using symptomatic leaf samples (15 days after inoculation) confirmed SimMV as the causal agent. To our knowledge, this is the first report of a SimMV isolate infecting P. vulgaris. This virus is apparently fast expanding its host range from Malvaceae to Solanaceae species and leguminous hosts after the introduction of B. tabaci biotype B (2). More extensive surveys are necessary to access the current epidemiological importance of SimMV in both snap and common beans in Brazil. References: (1) J.C. Faria and D. P. Maxwell. Phytopathology 89:262, 1999. (2) F. R. Fernandes et al. Arch. Virol. 154:1567, 2009. (3) M. R. Rojas et al. Plant Dis. 77:340, 1993.

scholarly journals First Report of Phytophthora Blight Caused by Phytophthora capsici on Snap Bean in New York

Plant Disease ◽  
2011 ◽  
Vol 95 (8) ◽  
pp. 1028-1028
Author(s):  
M. T. McGrath ◽  
J. Strauss ◽  
H. R. Dillard
Keyword(s):  
New York ◽  
Phytophthora Capsici ◽  
Infected Plant ◽  
Sodium Hypochlorite Solution ◽  
Distilled Water ◽  
Phytophthora Blight ◽  
Snap Bean ◽  
First Report ◽  
Stem Lesions ◽  
Zoospore Suspension

Phytophthora capsici Leonian is an important pathogen of solanaceous and cucurbit crops. Phytophthora blight was first reported on snap bean (Phaseolus vulgaris L.) in Michigan in 2003 (2) and Connecticut in 2010 (3). This report documents the discovery of P. capsici on snap bean (cv. Bronco) grown in Riverhead, NY in September 2008 and August 2010 on snap bean (cv. Valentino) in Holley, NY, more than 690 km away. Disease was favored by frequent rainfall and prolonged wet periods with air temperatures of 24 to 29°C. Both locations were commercial fields previously planted to pepper or cucurbits affected by P. capsici. In Riverhead, infected pods had characteristic yeast-like growth of P. capsici, which were predominantly sporangia. In Holley, large water-soaked lesions were observed on snap bean foliage, and as the disease progressed, leaves became necrotic and detached from the plant. Reddish brown lesions were observed on stems in advance of white areas of sporulation. Infected pods displayed white mycelial growth, were shriveled, and desiccated. P. capsici was isolated from symptomatic tissues. Stems and pods were surface disinfested for 3 min in 0.525% sodium hypochlorite solution, rinsed for 3 min in sterile distilled water, transferred to PARPH (4) media, and incubated at 22°C. After 5 days, hyphae from colony margins were excised and transferred to 15% unclarified V8 agar media. Cultures consisting of white mycelia and ovoid papillate sporangia on long pedicels were identified as P. capsici. Sporangia were 25.0 to 70.0 × 10.0 to 22.5 μm (average 42.0 × 16.25 μm). Identity was further confirmed by PCR primers specific to P. capsici (1). DNA was extracted from mycelia produced on V8 agar and amplification with the species-specific primers resulted in a PCR product of the same size as that obtained from a known isolate of P. capsici. Pathogenicity of the isolate from Holley was determined by two methods on 50-day-old snap bean plants (cv. Valentino) grown in a greenhouse. In method one, four plants were inoculated with 1-cm-diameter mycelial plugs excised from 8-day-old cultures. A single plug was placed against the stem at the soil line. Four control plants were treated similarly with noncolonized agar plugs. In method two, entire plants were atomized with 10 ml of a zoospore suspension (2.6 × 105/ml). Control plants were atomized with sterile distilled water. All plants were placed in a growth chamber with continuous mist for 24 h at 24°C. After 24 h, plants were enclosed in plastic bags and placed in a greenhouse at 27°C. Stem lesions similar to those observed in affected fields were evident on plants treated with mycelia plugs 2 days after inoculation. Plants inoculated with the zoospore suspension developed stem lesions and desiccated pods. Control plants were asymptomatic. P. capsici was successfully recovered from infected plant tissue, fulfilling Koch's postulates. The Riverhead isolate was demonstrated as pathogenic on snap bean and cucumber by placing colonized plugs on pods and fruit that were subsequently incubated in moist chambers (24°C, 90 to 100% relative humidity). P. capsici was successfully recovered from symptomatic pods and fruit. To our knowledge, this is the first report of Phytophthora blight caused by P. capsici on snap bean in New York. References: (1) A. R. Dunn et al. Plant Dis. 94:1461, 2010. (2) A. J. Gevens et al. Plant Dis. 92:201, 2008. (3) J. A. LaMondia et al. Plant Dis. 94:134, 2010. (4) G. C. Papavisas et al. Phytopathology 71:129, 1981.

scholarly journals First Report of Mosaic Disease Caused by Colombian datura virus on Solanum lycopersicum Plants Commercially Cultivated in Japan

Plant Disease ◽  
2014 ◽  
Vol 98 (5) ◽  
pp. 698-698 ◽  
Author(s):  
Y. Tomitaka ◽  
T. Usugi ◽  
R. Kozuka ◽  
S. Tsuda
Keyword(s):  
Nucleotide Sequence ◽  
Solanum Lycopersicum ◽  
Mosaic Disease ◽  
Causal Agent ◽  
Cultivated Plants ◽  
Rt Pcr ◽  
Degenerate Primers ◽  
Specific Primers ◽  
Tomato Plants ◽  
First Report

In 2009, some commercially grown tomato (Solanum lycopersicum) plants in Chiba Prefecture, Japan, exhibited mosaic symptoms. Ten plants from a total of about 72,000 cultivated plants in the greenhouses showed such symptoms. To identify the causal agent, sap from leaves of the diseased plants was inoculated into Chenopodium quinoa and Nicotiana benthamiana plants. Local necrotic lesions appeared on inoculated leaves of C. quinoa, but no systemic infection was observed. Systemic mosaic symptoms were observed on the N. benthamiana plants inoculated. Single local lesion isolation was performed three times using C. quinoa to obtain a reference isolate for further characterization. N. benthamiana was used for propagation of the isolate. Sap from infected leaves of N. benthamiana was mechanically inoculated into three individual S. lycopersicum cv. Momotaro. Symptoms appearing on inoculated tomatoes were indistinguishable from those of diseased tomato plants found initially in the greenhouse. Flexuous, filamentous particles, ~750 nm long, were observed by electron microscopy in the sap of the tomato plants inoculated with the isolate, indicating that the infecting virus may belong to the family Potyviridae. To determine genomic sequence of the virus, RT-PCR was performed. Total RNA was extracted from the tomato leaves experimentally infected with the isolate using an RNeasy Plant Mini kit (QIAGEN, Hilden, Germany). RT-PCR was performed by using a set of universal, degenerate primers for Potyviruses as previously reported (2). Amplicons (~1,500 bp) generated by RT-PCR were extracted from the gels using the QIAquick Gel Extraction kit (QIAGEN) and cloned into pCR-BluntII TOPO (Invitrogen, San Diego, CA). DNA sequences of three individual clones were determined using a combination of plasmid and virus-specific primers, showing that identity among three clones was 99.8%. A consensus nucleotide sequence of the isolate was deposited in GenBank (AB823816). BLASTn analysis of the nucleotide sequence determined showed 99% identity with a partial sequence in the NIb/coat protein (CP) region of Colombian datura virus (CDV) tobacco isolate (JQ801448). Comparison of the amino acid sequence predicted for the CP with previously reported sequences for CDV (AY621656, AJ237923, EU571230, AM113759, AM113754, and AM113761) showed 97 to 100% identity range. Subsequently, CDV infection in both the original and experimentally inoculated plants was confirmed by RT-PCR using CDV-specific primers (CDVv and CDVvc; [1]), and, hence, the causal agent of the tomato disease observed in greenhouse tomatoes was proved to be CDV. The first case of CDV on tomato was reported in Netherlands (3), indicating that CDV was transmitted by aphids from CDV-infected Brugmansia plants cultivated in the same greenhouse. We carefully investigated whether Brugmansia plants naturally grew around the greenhouses, but we could not find them inside or in proximity to the greenhouses. Therefore, sources of CDV inoculum in Japan are still unclear. This is the first report of a mosaic disease caused by CDV on commercially cultivated S. lycopersicum in Japan. References: (1) D. O. Chellemi et al. Plant Dis. 95:755, 2011. (2) J. Chen et al. Arch. Virol. 146:757, 2001. (3) J. Th. J. Verhoeven et al. Eur. J. Plant. Pathol. 102:895, 1996.

scholarly journals First Report of Common Bean (Phaseolus vulgaris) Stem Rot Caused by Colletotrichum spaethianum in China

Plant Disease ◽  
2019 ◽  
Vol 103 (1) ◽  
pp. 151
Author(s):  
L. Yang ◽  
X. H. Lu ◽  
Y. L. Jing ◽  
S. D. Li ◽  
B. M. Wu
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Stem Rot ◽  
First Report

scholarly journals Scheduling of irrigation in snap bean (Phaseolus vulgaris var. nanus) using canopy temperature

2005 ◽  
Vol 11 (1) ◽  
Author(s):  
L. Helyes ◽  
Z. Pék ◽  
Gy. Varga ◽  
J. Dimény
Keyword(s):  
Phaseolus Vulgaris ◽  
Water Supply ◽  
Surface Temperature ◽  
Yield Loss ◽  
Canopy Temperature ◽  
Water Turnover ◽  
Snap Bean ◽  
Snap Beans ◽  
Yield Production ◽  
Spring Sowing

The present paper evaluates the result of irrigation experiments carried out on snap beans sown in spring and summer and grown with and without irrigation. The experiments were run over the course of 12 years. In the average of 12 years, the yield was 2.8t ha-I for spring sown and 1.9 t ha-I in summer-sown plants without irrigation. The lowest level of profitable production, the 5.5t ha-I was reached twice in the case of spring sowing and only once in the case of summer sowing. Profitable yield production can be ensured only with regular irrigation and thus the yield may be increased by 4-5 times. In four of the twelve years we determined the canopy surface temperature of snap bean stands with and without irrigation. A Raynger II infrared remote thermometer determined the canopy surface temperature every day at 13.00 hours. The canopy temperature can well characterize the water supply of plant stands. This parameter may be used for describing the degree of drought and the water turnover of plant stands with different water supply. The positive values of foliage-air temperature differences (SDD) numerically express the degree of drought and the water supply of the crops. The results indicated that a 1 °C higher SDD value may cause 90-130 kg/ha yield loss.

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