Mapping and validation of a stable quantitative trait locus conferring maize resistance to Gibberella ear rot

Plant Disease ◽  
2021 ◽  
Author(s):  
Guangfei Zhou ◽  
Shunfa Li ◽  
Liang Ma ◽  
Fang Wang ◽  
Fuyan Jiang ◽  
...  
Keyword(s):  
Quantitative Trait ◽  
Interaction Analysis ◽  
Genetic Locus ◽  
Snp Array ◽  
Minor Effect ◽  
Ear Rot ◽  
Resistant Parent ◽  
Gibberella Ear Rot ◽  
Maize Varieties ◽  
Ril Population

Gibberella ear rot (GER), a prevalent disease caused by Fusarium graminearum (F. graminearum), can result in significant yield loss and carcinogenic mycotoxin contamination in maize worldwide., However, only a few quantitative trait loci (QTLs) for GER resistance have been reported. In this study, we evaluated a Chinese recombinant inbred line (RIL) population comprising of 204 lines, developed from a cross between a resistant parent DH4866 and a susceptible line T877, in three field trial environments under artificial inoculation with F. graminearum. The RIL population and their parents were genotyped with an Affymetrix microarray CGMB56K SNP Array. Based on the genetic linkage map constructed using 1,868 bins as markers, eleven QTLs, including five stable QTLs, were identified by individual environment analysis. Joint multiple environments analysis and epistatic interaction analysis revealed six additive and six epistatic (additive × additive) QTLs, respectively. None of the QTLs could explain more than 10% of phenotypic variation, suggesting that multiple minor-effect QTLs contributed to the genetic component of resistance to GER, and both additive and epistatic effects contributed to the genetic architecture of resistance to GER. A novel QTL, qGER4.09, with largest effect, identified and validated using 588 F2 individuals, was colocalized with genomic regions for FER and Aspergillus ear rot, indicating that this genetic locus likely confers resistance to multiple pathogens, and can be potentially utilized in breeding maize varieties, aimed at improving the resistance not only to GER, but also other ear rot diseases.

scholarly journals Identification of a major quantitative trait locus, qGER4.2, conferring resistance to Gibberella ear rot in maize using a Chinese recombination inbred line population

2020 ◽  
Author(s):  
Guangfei Zhou ◽  
Shunfa Li ◽  
Liang Ma ◽  
Fang Wang ◽  
Yali Sun ◽  
...  
Keyword(s):  
Inbred Line ◽  
Quantitative Trait ◽  
Snp Array ◽  
Ear Rot ◽  
Maize Breeding ◽  
Resistant Parent ◽  
Gibberella Ear Rot ◽  
Prevalent Disease ◽  
Stable Qtls ◽  
Trait Locus

Abstract Background: Gibberella ear rot (GER), a prevalent disease caused by Fusarium graminearum, can result in yield loss and mycotoxin contamination in maize. Despite that several QTLs related to GER resistance have been reported previously, few of them was identified in Chinese maize inbred lines. In this study, we employed a Chinese recombination inbred line (RIL) population comprising of 204 lines, developed from a cross involving a resistant parent DH4866 and a susceptible line T877. The population was phenotypically evaluated at three field trial locations under artificial inoculation with F. graminearum and genotyped with an Affymetrix microarray CGMB56K SNP Array to detect the quantitative trait loci (QTLs) for resistance to GER. Results: Based on the genetic linkage map constructed using 1,868 bins as markers, a total of 15 QTLs were identified, and both DH4866 and T877 alleles at these QTLs contributed toward resistance. Of these QTLs, five were stably expressed across multiple locations, including three co-localized with previously reported genomic regions. The largest-effect QTL located on chromosome 4, qGER4.2, which accounted for 5.66~17.10% of the phenotypic variation, was detected at all locations. Based on the phenotypic values of the alleles corresponding to the five stable QTLs, a significant correlation (r2 = 0.45) between resistance to GER and the number of resistant alleles was observed. Conclusions: QTL mapping was successfully employed to identify genetic loci conferring resistance to GER by a high-density genetic map constructed from two Chinese maize germplasms. The five stable QTLs identified, especially the large-effect QTL, qGER4.2, will be useful in maize breeding programs aimed at improving GER resistance.

Low validation rate of quantitative trait loci for Gibberella ear rot resistance in European maize

2016 ◽  
Vol 130 (1) ◽  
pp. 175-186 ◽  
Author(s):  
Pedro Correa Brauner ◽  
Albrecht E. Melchinger ◽  
Tobias A. Schrag ◽  
H. Friedrich Utz ◽  
Wolfgang Schipprack ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Ear Rot ◽  
Gibberella Ear Rot ◽  
Validation Rate ◽  
Trait Loci ◽  
European Maize

Molecular mapping of QTLs for resistance to Gibberella ear rot, in corn, caused by Fusarium graminearum

Genome ◽  
2005 ◽  
Vol 48 (3) ◽  
pp. 521-533 ◽  
Author(s):  
M Liakat Ali ◽  
Jeff H Taylor ◽  
Liu Jie ◽  
Genlou Sun ◽  
Manilal William ◽  
...  
Keyword(s):  
Linkage Group ◽  
Linkage Map ◽  
Fusarium Graminearum ◽  
Molecular Mapping ◽  
Average Distance ◽  
Interval Mapping ◽  
Ear Rot ◽  
Resistant Parent ◽  
Gibberella Ear Rot ◽  
Group 5

Gibberella ear rot, caused by the fungus Fusarium graminearum Schwabe, is a serious disease of corn (Zea mays) grown in northern climates. Infected corn is lower yielding and contains toxins that are dangerous to livestock and humans. Resistance to ear rot in corn is quantitative, specific to the mode of fungal entry (silk channels or kernel wounds), and highly influenced by the environment. Evaluations of ear rot resistance are complex and subjective; and they need to be repeated over several years. All of these factors have hampered attempts to develop F. graminearum resistant corn varieties. The aim of this study was to identify molecular markers linked to the genes for resistance to Gibberella ear rot. A recombinant inbred (RI) population, produced from a cross between a Gibberella ear rot resistant line (CO387) and a susceptible line (CG62), was field-inoculated and scored for Gibberella ear rot symptoms in the F4, F6, and F7 generations. The distributions of disease scores were continuous, indicating that resistance is probably conditioned by multiple loci. A molecular linkage map, based on segregation in the F5 RI population, contained 162 markers distributed over 10 linkage groups and had a total length of 2237 cM with an average distance between markers of 13.8 cM. Composite interval mapping identified 11 quantitative trait loci (QTLs) for Gibberella ear rot resistance following silk inoculation and 18 QTLs following kernel inoculation in 4 environments that accounted for 6.7%–35% of the total phenotypic variation. Only 2 QTLs (on linkage group 7) were detected in more than 1 test for silk resistance, and only 1 QTL (on linkage group 5) was detected in more than 1 test for kernel resistance, confirming the strong influence of the environment on these traits. The majority of the favorable alleles were derived from the resistant parent (CO387). The germplasm and markers for QTLs with significant phenotypic effects may be useful for marker-assisted selection to incorporate Gibberella ear rot resistance into commercial corn cultivars.Key words: Gibberella ear rot, corn, Fusarium graminearum, QTL, linkage map.

scholarly journals Quantitative trait loci for Fusarium and Gibberella ear rot resistance in Argentinian maize germplasm

Euphytica ◽  
2016 ◽  
Vol 211 (3) ◽  
pp. 287-294 ◽  
Author(s):  
Gerardo M. Giomi ◽  
Enrique D. Kreff ◽  
Juliana Iglesias ◽  
Carolina M. Fauguel ◽  
Mariana Fernandez ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Ear Rot ◽  
Gibberella Ear Rot ◽  
Maize Germplasm ◽  
Trait Loci

Phytotoxic effect of deoxynivalenol and gibberella ear rot resistance of com

1995 ◽  
Vol 3 (5) ◽  
pp. 383-388 ◽  
Author(s):  
Françoise Cossette ◽  
J. David Miller
Keyword(s):  
Ear Rot ◽  
Gibberella Ear Rot ◽  
Phytotoxic Effect

scholarly journals Metabolome-scale genome-wide association studies reveal chemical diversity and genetic control of maize specialized metabolites

2018 ◽  
Author(s):  
Zhou Shaoqun ◽  
Karl A. Kremling ◽  
Bandillo Nonoy ◽  
Richter Annett ◽  
Ying K. Zhang ◽  
...  
Keyword(s):  
Quantitative Trait ◽  
Citrate Synthase ◽  
Association Studies ◽  
Inbred Lines ◽  
Chemical Diversity ◽  
Genome Wide Association ◽  
Genome Wide Association Studies ◽  
Single Linkage ◽  
Genome Wide ◽  
Maize Varieties

One Sentence SummaryHPLC-MS metabolite profiling of maize seedlings, in combination with genome-wide association studies, identifies numerous quantitative trait loci that influence the accumulation of foliar metabolites.AbstractCultivated maize (Zea mays) retains much of the genetic and metabolic diversity of its wild ancestors. Non-targeted HPLC-MS metabolomics using a diverse panel of 264 maize inbred lines identified a bimodal distribution in the prevalence of foliar metabolites. Although 15% of the detected mass features were present in >90% of the inbred lines, the majority were found in <50% of the samples. Whereas leaf bases and tips were differentiated primarily by flavonoid abundance, maize varieties (stiff-stalk, non-stiff-stalk, tropical, sweet corn, and popcorn) were differentiated predominantly by benzoxazinoid metabolites. Genome-wide association studies (GWAS), performed for 3,991 mass features from the leaf tips and leaf bases, showed that 90% have multiple significantly associated loci scattered across the genome. Several quantitative trait locus hotspots in the maize genome regulate the abundance of multiple, often metabolically related mass features. The utility of maize metabolite GWAS was demonstrated by confirming known benzoxazinoid biosynthesis genes, as well as by mapping isomeric variation in the accumulation of phenylpropanoid hydroxycitric acid esters to a single linkage block in a citrate synthase-like gene. Similar to gene expression databases, this metabolomic GWAS dataset constitutes an important public resource for linking maize metabolites with biosynthetic and regulatory genes.

scholarly journals Molecular Insights into Defense Responses of Vietnamese Maize Varieties to Fusarium verticillioides Isolates

2021 ◽  
Vol 7 (9) ◽  
pp. 724
Author(s):  
Trang Minh Tran ◽  
Maarten Ameye ◽  
Sofie Landschoot ◽  
Frank Devlieghere ◽  
Sarah De Saeger ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Fusarium Verticillioides ◽  
Gene Expression Profiles ◽  
Defense Responses ◽  
Ear Rot ◽  
In Planta ◽  
Resistant Lines ◽  
Maize Varieties ◽  
Pathogenesis Related ◽  
Related Proteins

Fusarium ear rot (FER) caused by Fusarium verticillioides is one of the main fungal diseases in maize worldwide. To develop a pathogen-tailored FER resistant maize line for local implementation, insights into the virulence variability of a residing F. verticillioides population are crucial for developing customized maize varieties, but remain unexplored. Moreover, little information is currently available on the involvement of the archetypal defense pathways in the F. verticillioides–maize interaction using local isolates and germplasm, respectively. Therefore, this study aims to fill these knowledge gaps. We used a collection of 12 F. verticillioides isolates randomly gathered from diseased maize fields in the Vietnamese central highlands. To assess the plant’s defense responses against the pathogens, two of the most important maize hybrid genotypes grown in this agro-ecological zone, lines CP888 and Bt/GT NK7328, were used. Based on two assays, a germination and an in-planta assay, we found that line CP888 was more susceptible to the F. verticillioides isolates when compared to line Bt/GT NK7328. Using the most aggressive isolate, we monitored disease severity and gene expression profiles related to biosynthesis pathways of salicylic acid (SA), jasmonic acid (JA), abscisic acid (ABA), benzoxazinoids (BXs), and pathogenesis-related proteins (PRs). As a result, a stronger induction of SA, JA, ABA, BXs, and PRs synthesizing genes might be linked to the higher resistance of line Bt/GT NK7328 compared to the susceptible line CP888. All these findings could supply valuable knowledge in the selection of suitable FER resistant lines against the local F. verticllioides population and in the development of new FER resistant germplasms.

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