scholarly journals Elucidation of the Roles of Blackcurrant reversion virus and Phytoplasma in the Etiology of Full Blossom Disease in Currants

Plant Disease ◽  
2009 ◽  
Vol 93 (8) ◽  
pp. 832-838 ◽  
Author(s):  
Josef Špak ◽  
Darina Kubelková ◽  
Jaroslava Přibylová ◽  
Vlastimila Špaková ◽  
Karel Petrzik
Keyword(s):  
Causal Agent ◽  
Black Currant ◽  
Aster Yellows ◽  
Reversion Virus ◽  
Aster Yellows Phytoplasma ◽  
Yellow Petal ◽  
Red Currant

To determine the roles of phytoplasmas and Blackcurrant reversion virus (BRV) in the etiology of full blossom disease (FBD), we conducted graft and dodder transmission experiments. Scions from FBD-affected Ribes rubrum were grafted onto red currants, white currants, and black currants. Red and white cultivars revealed symptoms of FBD, whereas blackcurrant displayed symptoms of BRV. No differences in symptoms were observed between plants infected with BRV only and those infected with BRV and phytoplasma. Aster yellows phytoplasma subgroup 16SrI-C was transferred from FBD-infected red currants to periwinkle, where symptoms of green and yellow petal were observed. Back-transmission of phytoplasma to currant seedlings of red and black currant was not successful. Scions of periwinkle infected with aster yellows phytoplasmas of subgroup 16SrI-C and 16SrI-B, which were bottle-, bark-, and approach-grafted onto seedlings of red and black currant, resulted in positive but symptomless transmission of phytoplasma to red currant. We conclude that FBD symptoms are induced by BRV rather than by phytoplasma, which was originally described as the causal agent of FBD.

scholarly journals Improved PCR Detection of Blackcurrant reversion virus in Ribes and Further Evidence that It Is the Causal Agent of Reversion Disease

Plant Disease ◽  
2002 ◽  
Vol 86 (12) ◽  
pp. 1333-1338 ◽  
Author(s):  
A. Teifion Jones ◽  
Wendy J. McGavin
Keyword(s):  
Causal Agent ◽  
Pcr Detection ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Flower Bud ◽  
Annealing Temperatures ◽  
Reversion Virus ◽  
Polymerase Chain ◽  
Triton X ◽  
Red Currant

Within 5 years of mechanically inoculating blackcurrant cultivars with partially purified preparations of particles of Blackcurrant reversion virus (BRV), infected plants developed leaf and flower bud symptoms typical of reversion disease, demonstrating that BRV is the causal agent of this disease. To improve the erratic immunocapture reverse transcriptase-polymerase chain reaction (RT-PCR) detection of BRV in Ribes plants, various stepwise changes were made to the original protocol. Significant improvement in the reliability and sensitivity of BRV detection was made by extracting RNA from trapped BRV particles using Triton-X 100, the design of new primers with higher annealing temperatures, and the use of ‘Ready-to-go’ RT-PCR beads. These features, combined with other minor changes to the protocol, improved BRV detection in reverted blackcurrant plants from <50% to >90% but the reliability of BRV detection in red currant was always very much less and was possible only using nested PCR that was developed for this purpose.

NEW VARIETIES OF CURRANTS AND GOOSEBERRY BREEDING PLANTS

1970 ◽  
pp. 41-46
Author(s):  
O. D. Golyaeva ◽  
O. V. Kurashev ◽  
S. D. Knyazev ◽  
А. Yu. Bakhotskaya
Keyword(s):  
Powdery Mildew ◽  
Genetic Basis ◽  
Black Currant ◽  
Leaf Spots ◽  
Agricultural Sciences ◽  
Berry Crops ◽  
New Varieties ◽  
First Time ◽  
Red Currant

The main goal of the scientific institution was and remains to improve the assortment of fruit and berry crops for the development of domestic horticulture. Black currant breeding at VNIISPK was started by A.F Tamarova and continued by the doctor of agricultural Sciences T.P.Ogoltsova and doctor of agricultural Sciences S.D. Knyazev. A long-term breeding program has been developed. The main goals of the program are to create black currant cultivars with continuous resistance to diseases, first of all powdery mildew, as wells resistance to pests, i.e. bud mite. As a result of the long-term work, over 40 black currant cultivars have been developed, 14 of them are zoned. Red currant breeding was led by the candidate of agricultural Sciences L.V. Bayanova; since 2001 the work has been continued by the candidate of agricultural Sciences O.D. Golyaeva. ‘Heinemanns Rote Spӓtlese’, the descendant of R. multiflorum Kit., was involved in the red currant breeding for the first time in Russia. On its genetic basis, a series of late maturing cultivars with long and dense racemes was created. At the Institute, in total 21cultivars of red currants have been developed, 13 of them are zoned. At present, red currant cultivars make up 25.5% of the zoned assortment in Russia. The first research on gooseberries was stated by V.P. Semakin and A.F Tamarova; since 1992 the systematic gooseberry breeding has been carried out by the candidate of agricultural Sciences O.V. Kurashev. On the basis of Grossularia robusta, we have created gooseberry forms that are resistant to powdery mildew and leaf spots. These forms are highly productive, weakly thorned, having bush habit suitable for mechanized harvest. The result of breeding activities was the transfer of 6 gooseberry cultivars to State agricultural testing: ‘Solnechny Zaychik’, ‘Nekrasovsky’, ‘Yupiter’, ‘Zemlianichny’, ‘Moryachok’ and ‘Discovery’.

Fungus Spaeropsis Malorum peck. One of the currant stalk drying agents

2020 ◽  
Vol 62 ◽  
pp. 172-178
Author(s):  
S. E. Golovin
Keyword(s):  
Causative Agent ◽  
High Frequency ◽  
Frequency Of Occurrence ◽  
Ribes Nigrum ◽  
Black Currant ◽  
Causative Agents ◽  
Pear Trees ◽  
Red Currant

The article highlights the results of research carried out in 2019-2020 to study the reasons for the drying out of the stems of black currant (Ribes nigrum L.), red currant (Ribes rubrum L.) and golden currant (Ribes aureum L.). As a result of research, the fungus Sphaeropsis malorum Peck. in the first in Russia it was revealed from the drying stems of three types of black currants, red currants and golden currants. This  pathogen inhabited currant stems together with fungi from the genus Cytospora spp., causative agents of stem drying. On red currants, it was established that S. malorum was the main causative agent of branch drying, as indicated by the high frequency of occurrence (75.0-83.3 %) of this fungus on the affected stems, compared with species from the genus Cytospora spp. (16.7-33.3 %) and Phomopsis ribesia (25.0 %). Due to the fact that the cultivation of currants in the immediate vicinity of apple and pear trees leads to infection of plants with the fungus S. malorum, it is necessary to avoid planting currants in the vicinity of fruit-bearing seed gardens.

Eutypa lata, the causal agent of dieback in red currant (Ribes rubrum) and gooseberry (R. uva-crispa) in the Netherlands

2011 ◽  
Vol 131 (3) ◽  
pp. 441-449 ◽  
Author(s):  
Marcel Wenneker ◽  
Marcel M. J. P. van Raak ◽  
Ilse R. van Brouwershaven ◽  
Wendy Martin ◽  
Linda F. F. Kox
Keyword(s):  
The Netherlands ◽  
Causal Agent ◽  
Eutypa Lata ◽  
Red Currant

scholarly journals Seasonal Patterns of Aster Leafhopper (Hemiptera: Cicadellidae) Abundance and Aster Yellows Phytoplasma Infectivity in Wisconsin Carrot Fields

2013 ◽  
Vol 42 (3) ◽  
pp. 491-502 ◽  
Author(s):  
K. E. Frost ◽  
P. D. Esker ◽  
R. Van Haren ◽  
L. Kotolski ◽  
R. L. Groves
Keyword(s):  
Seasonal Patterns ◽  
Aster Yellows ◽  
Aster Leafhopper ◽  
Aster Yellows Phytoplasma

scholarly journals Chemical and sensorial characteristics of fruit spirits produced from different black currant (Ribes nigrum l.) and red currant (Ribes rubrum l.) cultivars

2012 ◽  
Vol 31 (2) ◽  
pp. 217 ◽  
Author(s):  
Todor Vulić ◽  
Ninoslav Nikićević ◽  
Ljubiša Stanković ◽  
Milovan Veličković ◽  
Marina Todosijević ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Sensory Evaluation ◽  
Chemical Characteristics ◽  
Ribes Nigrum ◽  
Black Currant ◽  
Fruit Spirits ◽  
Red Currant

Chemical characteristics of fruit spirits produced from three black currant cultivars (Malling Juel, Ometa, Ben Sarek) and two red currant cultivars (Rondom and Versailles) grown in Serbia were examined using gas chromatography. In addition, sensory evaluation of the spirit samples was performed using the Buxbaum model of positive rating. Fruit spirits produced from black currant cultivars displayed the highest contents of methanol (267.2 - 595.0 g/hl A. A) and amyl alcohols (74.2 - 204.8 g/hl A. A). Total sensory scores of currant brandies ranged between 17.60 and 18.40, while currant brandy produced from Ben Sarek black currant cultivar received the highest score (18.40). All tested samples of currant brandies fulfilled EU requirements as regards their content of methanol and other components such as acetaldehyde, ethyl acetate and high alcohols. The currant distillates were compared with other commercial spirits, including plum and grape brandies.

scholarly journals First Report of an Aster Yellows Phytoplasma Associated with Cabbage in Southern Texas

Plant Disease ◽  
2001 ◽  
Vol 85 (4) ◽  
pp. 447-447 ◽  
Author(s):  
I.-M. Lee ◽  
R. A. Dane ◽  
M. C. Black ◽  
Noel Troxclair
Keyword(s):  
16S Rdna ◽  
Nested Pcr ◽  
Restriction Enzymes ◽  
Diagnostic Procedures ◽  
Acid Extraction ◽  
Insect Vector ◽  
Aster Yellows ◽  
First Report ◽  
Pcr Products ◽  
Aster Yellows Phytoplasma

In early spring 2000 carrot crops in southwestern Texas were severely infected by an outbreak of phyllody associated with aster yellows phytoplasma. Cabbage crops that had been planted adjacent to these carrot fields began to display previously unobserved symptoms characteristic of phytoplasma infection. Symptoms included purple discoloration in leaf veins and at the outer edges of leaves on cabbage heads. Proliferation of sprouts also occurred at the base of the stem and between leaf layers of some plants, and sprouts sometimes continued to proliferate on extended stems. About 5% of cabbage plants in the field exhibited these symptoms. Two symptomless and four symptomatic cabbage heads were collected in early April from one cabbage field. Veinal tissues were stripped from each sample and used for total nucleic acid extraction. To obtain specific and sufficient amount of PCR products for analysis, nested PCR was performed by using primer pairs (first with P1/P7 followed by R16F2n/R16R2) (1,2) universal for phytoplasma detection. A specific 16S rDNA fragment (about 1.2 kb) was strongly amplified from the four symptomatic but not from the two asymptomatic samples. The nested PCR products obtained from the four symptomatic samples were then analyzed by restriction fragment length polymorphism (RFLP) using the restriction enzymes MseI, HhaI, and HpaII, and the RFLP patterns were compared to the published patterns of known phytoplasmas (1). The resulting RFLP patterns were identical to those of a phytoplasma belonging to subgroup B of the aster yellows phytoplasma group (16SrI). These RFLP patterns were also evident in putative restriction sites observed in a 1.5 kbp nucleotide sequence of the 16S rDNA. This is the first report of aster yellows phytoplasma associated disease symptoms in cabbage in Texas. The occurrence of cabbage proliferation coincided with the presence of high populations of the insect vector, aster leafhopper. References: (1) I.-M. Lee et al. Int. J. Syst. Bacteriol. 48:1153, 1998. (2) B. Schneider et al. 1995. Molecular and Diagnostic Procedures in Mycoplasmology, Vol. I. Academic Press, San Diego, CA.

Blackcurrant reversion virus. [Distribution map].

2002 ◽  
Author(s):  
Keyword(s):  
New Zealand ◽  
Geographical Distribution ◽  
Ribes Nigrum ◽  
Distribution Map ◽  
Central Russia ◽  
Reversion Virus ◽  
Virus Distribution ◽  
New Distribution ◽  
Distribution In Europe ◽  
Red Currant

Abstract A new distribution map is provided for Blackcurrant reversion virus Viruses: Comoviridae: Nepovirus Hosts: Blackcurrant (Ribes nigrum) and red currant (R. rubrum). Information is given on the geographical distribution in EUROPE, Austria, Belarus, Denmark, Estonia, Finland, France, Germany, Hungary, Netherlands, Poland, Central Russia Russia, Sweden, Switzerland, UK, OCEANIA, New Zealand.

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