scholarly journals A New Host Diagnosed with a Strain of Sugarcane mosaic virus in Florida: Red-Veined Prayer Plant (Maranta leuconeura erythroneura)

Plant Disease ◽  
2010 ◽  
Vol 94 (3) ◽  
pp. 378-378 ◽  
Author(s):  
C. A. Baker ◽  
L. J. Wilber ◽  
L. Jones
Keyword(s):  
Mosaic Virus ◽  
Ground Cover ◽  
Datura Stramonium ◽  
Sugarcane Mosaic Virus ◽  
Chenopodium Amaranticolor ◽  
New Host ◽  
Three Samples ◽  
Food Agric ◽  
Direct Elisa ◽  
Viral Inclusions

Prayer plants (Maranta spp.), which are indigenous to Brazil, are commercially produced in nurseries mainly to be sold as potted houseplants. However, in frost-free areas, they are also used as a ground cover. In March 2009, the Division of Plant Industry in Gainesville, FL received cuttings of red-veined prayer plant or red maranta (Maranta leuconeura erythroneura) from several nurseries in central Florida. The cuttings originated in Costa Rica. The presence of a viral infection was indicated by the mosaic pattern seen on the upper surface of the leaves and chlorotic lesions on the underside of leaves. Epidermal strips were taken and stained in Orange-Green (O-G) and Azure A (1). Microscopic examination revealed viral inclusions that stained only in O-G, indicating the presence of a potyvirus. Leaf dips were prepared for the electron microscope and flexuous rods consistent with a potyvirus were found. Indirect-ELISA using universal potyvirus antiserum (Agdia Inc., Elkhart, IN) confirmed the presence of a potyvirus infection. Total RNA was extracted from symptomatic tissue of five infected samples with Qiagen's RNeasy Plant Mini Kit (Qiagen Inc., Valencia, CA). Reverse transcription was conducted with the oligo d(T) primer M4T with AMV-RT at 37°C for 1 h. PCR was performed with primer M4 and a degenerate primer designed to amplify the 3′ end of all potyviruses (2). A target amplicon of 1.7 kb was produced from all five samples. Three of these samples were cloned and sequenced. Approximately 1,250 bp were sequenced from each sample. The sequenced regions include the 3′ end of the Nib gene, the complete coat protein, and the beginning of the 3′ untranslated region (UTR). The nucleotide (nt) sequences were deposited into GenBank (Accession Nos. GQ853403–GQ853405). The nt sequences of the three samples were 97 to 98% identical to each other. When compared with other potyviruses in the GenBank, the samples showed closest nt identity, 92 to 93%, with several isolates of Sugarcane mosaic virus (AJ278405, AY836523, U57357, and U57356). Of the plant species mechanically inoculated (Chenopodium amaranticolor, C. quinoa, Datura stramonium, Gomphrena globosa, Nicotiana benthamiana, and Zea mays), only Z. mays (corn) showed symptoms (a mild mosaic). The same type of viral inclusions were seen in leaf strips of infected corn as in the Maranta. The corn plant reacted positively in direct-ELISA against antiserum to Sugarcane mosaic virus (Agdia Inc.). Cuttings of infected Maranta were observed in the greenhouse and indoor situations for several months. The plants infected with Sugarcane mosaic virus lacked vigor and most eventually died. ELISA tests done on a few surviving plants were positive for Sugarcane mosaic virus, but the results were inconsistent, indicating a possible low titer of virus in the plants as they were dying. To our knowledge, this is a new host for Sugarcane mosaic virus, but it does not appear that Maranta will become a significant new reservoir of this virus for sugarcane or corn growers. However, infected cuttings can greatly decrease the production of this plant as an ornamental. References: (1) J. R. Edwardson and R. G. Christie. Univ. Fla. Inst. Food Agric. Sci. Bull. 894, 1966. (2) A. Gibbs and A. Mackenzie. J. Virol. Methods 63:9, 1997.

scholarly journals First Report of Alfalfa mosaic virus in Pachysandra terminalis in Europe

Plant Disease ◽  
2000 ◽  
Vol 84 (5) ◽  
pp. 594-594 ◽  
Author(s):  
L. Cardin ◽  
B. Moury
Keyword(s):  
Electron Microscopy ◽  
Mosaic Virus ◽  
Vigna Unguiculata ◽  
Alfalfa Mosaic Virus ◽  
Ground Cover ◽  
Aphid Transmission ◽  
Plant Viruses ◽  
Aphid Species ◽  
Chenopodium Amaranticolor ◽  
New Host

Pachysandra terminalis (Buxaceae) was introduced from Japan to Europe in 1882. This ornamental plant is grown in northern Europe as a ground cover in shaded sites. Line patterns, more or less necrotic ringspots, and mosaic symptoms on leaves of pachysandra plants have been seen in public gardens in France (Strasbourg, Colmar, Mulhouse, and Nantes) and Germany (Freiburg im Breisgaü). Extracts of plant tissues obtained from these five sites were used for mechanical- and aphid-transmission experiments, enzyme-linked immunosorbent assays (ELISA) with antibodies directed toward a tomato strain of Alfalfa mosaic virus (AMV) (from G. Marchoux, INRA, France), and electron microscopy. All inoculations produced symptoms typical for AMV in Nicotiana tabacum cv. Xanthi-nc tobacco, Chenopodium amaranticolor, C. quinoa, Vigna unguiculata, Phaseolus vulgaris, Vicia faba, Pisum sativum, and Ocimum basilicum (2). Moreover, Medicago arborea, a new host for AMV, showed systemic mosaic on leaflets. On these and the original pachysandras, AMV was readily detected by ELISA. After isolation from three to four local lesions on Vigna unguiculata and further multiplication in tobacco, one isolate was purified. Bacilliform particles of three sizes, typical for AMV, were seen by electron microscopy. Transmissions of the strain to ELISA-negative pachysandras was achieved by mechanical inoculations (7 of 20 inoculated plants were ELISA positive) and by the aphid species Myzus persicae and Aphis craccivora (14 of 17 inoculated plants were infected). Symptoms were observed 2 months after inoculation; some of the plants remained symptomless but were AMV positive in ELISA. As early as 1970, similar symptoms were reported in pachysandra in New Jersey, and AMV was isolated from affected plants (1). However, inoculations of healthy pachysandra plants with AMV was not performed. Our results show the need for an AMV indexing protocol in the propagation of pachysandra to control its spread. References: (1) D. E. Hershman and E. H. Varney. Plant Dis. 66:1195, 1982. (2) E. M. J. Jaspars and L. Bos. 1980. Alfalfa mosaic virus. No. 229 in: Descriptions of Plant Viruses. Commonw. Mycol. Inst./Assoc. Appl. Biol., Kew, England.

scholarly journals First Report of Pepino mosaic virus on Tomato in Spain

Plant Disease ◽  
2001 ◽  
Vol 85 (12) ◽  
pp. 1292-1292 ◽  
Author(s):  
C. Jordá ◽  
A. Lázaro Pérez ◽  
P. Martínez-Culebras ◽  
P. Abad ◽  
A. Lacasa ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Canary Islands ◽  
Plant Protection ◽  
Polyclonal Antibodies ◽  
Datura Stramonium ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chenopodium Amaranticolor ◽  
Pepino Mosaic Virus ◽  
Tomato Plants ◽  
First Report

At the beginning of 2000, a damaging disease developed on protected tomato (Lycopersicon esculentum) crops grown in polyethylene greenhouses in different regions of Spain. Production losses were estimated at 15 to 80%. The tomato plants showed a variety of symptoms. The most common symptoms were leaf distortion, chlorosis, and mosaic. Some plants showed a dark green mosaic and bubbling of the leaf surface. Green striations were also observed on the stem and sepals. Most of the diseased plants had discolored fruits. Symptoms decreased as environmental temperature increased. The involvement of Pepino mosaic virus (PepMV) was suspected. To identify the etiological agent, ≈500 symptomatic tomato plants were collected from several locations in Alicante, Murcia, Almeria and the Canary Islands. Flexuous viral particles 510 nm long were observed by transmission electron microscopy, suggesting the presence of a potexvirus in the tissue extracts analyzed. All samples were tested by ELISA (enzyme-linked immunosorbent assay), using polyclonal antibodies to Narcissus mosaic virus (Adgen, Auchincriuve, Scotland), a virus serologically related to PepMV, and two antisera specific to PepMV (Adgen, Scotland and DMSZ, Braunschweig, Germany). PepMV was detected in 35% of the samples. Like PepMV, the virus infected (as confirmed by ELISA) greenhouse-grown Datura stramonium, Nicandra physalodes, Nicotiana benthamiana, N. clevelandii, Solanum tuberosum, and Vigna sinensis and did not infect Capsicum anuum, Cucumis sativus, Chenopodium amaranticolor, C. quinoa, Petunia × hybrida, Phaseolus vulgaris, Physalis floridana, N. glutinosa, N. rustica, or N. tabacum. The virus did infect Gomphrena globosa, which normally is not infected by PepMV. The first report of PepMV was on pepino (Solanum muricatum) in Peru in 1974 (1), but this virus has been recently reported in the Netherlands, England, Germany, and France on protected tomato crops (2). To our knowledge, this is the first report of PepMV in Spain, including the Canary Islands. References: (1) R. A. C. Jones et al. Ann. Appl. Biol. 94:61, 1980. (2) European and Mediterranean Plant Protection Organisation (EPPO). Alert List Viruses. On-line publication/2000/003.

scholarly journals Manutenção da infectividade de Tymovírus em extratos de plantas

1992 ◽  
Vol 6 (2) ◽  
pp. 15-26 ◽  
Author(s):  
Maria Mércia Barradas ◽  
Fernando J. Sanhueza Salas ◽  
Ivan P. González Buitrón
Keyword(s):  
Lycopersicon Esculentum ◽  
Mosaic Virus ◽  
Datura Stramonium ◽  
Chenopodium Amaranticolor ◽  
Nicotiana Glutinosa

Quatro isolados do vírus do mosaico da berinjela (EMV - "eggplant mosaic virus" - grupo tymovírus) foram armazenados a partir de extratos foliares de hospedeiras com sintomas sistêmicos. Os virus EMV-Al (isolado de Abelia), EMV-Sc (isolado da Escócia), -ts (estirpe-padrão) e VNBT (vírus da necrose branca do tomateiro), que induzem sintomas em Chenopodium amaranticolor, C. murale, C. quinoa (Família Chenopodiaceae) Datura stramonium, Lycopersicon esculentum e Nicotiana glutinosa (Solanaceae), foram conservados em extratos destas plantas, à temperatura ambiente, em geladeira e em congelador. A infectividade dos vírus, em diferentes períodos de armazenamento, foi testada em plantas de datura e glutinosa, para se determinar a longevidade in vitro. Constatou-se que, quando guardados em baixas temperaturas,os extratos preservam por mais tempo a infectividade dos vírus. No caso de datura e glutinosa, por exemplo, resultados positivos foram obtidos até 413 e 282 dias de armazenamento, respectivamente, em congelador. Entretanto, com relação às espécies de Chenopodium testadas, mesmo alguns extratos recém-preparados conduziram a resultados negativos, confirmando a presença de inibidores de infecção viral nestas plantas. Das três espécies, é sugerida a utilização apenas de C.quinoa para o preparo de extratos visando preservar estes vírus e, assim mesmo, por um período relativamente curto (entre 53 e 80 dias). A avaliação geral dos resultados mostra que, para os tymovírus estudados neste trabalho, é possível conservar a infectividade através da técnica de armazenamento de extratos foliares de plantas sistemicamente infectadas.

scholarly journals Cayaponia tibiricae: New Host of Zucchini Yellow Mosaic Virus in Brazil

Plant Disease ◽  
1999 ◽  
Vol 83 (5) ◽  
pp. 486-486 ◽  
Author(s):  
V. A. Yuki ◽  
J. A. M. Rezende ◽  
E. W. Kitajima ◽  
P. A. V. Barroso ◽  
H. Kuniyuki ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Electron Microscopic Examination ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Secondary Forests ◽  
Electron Microscopic ◽  
Chenopodium Amaranticolor ◽  
New Host

Cayaponia tibiricae Cogn. (CT) is a wild Cucurbitaceae species found in secondary forests in the State of São Paulo, Brazil. The species has indefinite growth and bears oblong dark green fruits, 15 to 20 mm long (1,2). CT plants showing yellow mosaic symptoms were found in Atibaia County. Extracts from symptomatic plants were rub inoculated to zucchini squash (Cucurbita pepo L.) and Chenopodium amaranticolor Coste & Reyn. Zucchini squash plants developed severe yellow mosaic with intense leaf malformation, while C. amaranticolor showed necrotic local lesions. Extracts from naturally infected CT, zucchini squash, and C. amaranticolor were tested by plate trapped antigen-enzyme-linked immunosorbent assay (PTA-ELISA) with antisera against papaya ringspot virus type W (PRSV-W), zucchini yellow mosaic virus (ZYMV), zucchini lethal chlorosis virus (ZLCV), watermelon mosaic virus 2 (WMV-2), and cucumber mosaic virus (CMV). All samples were positive in PTA-ELISA only with ZYMV antiserum. Also, in Western blot (immunoblot) assay, ZYMV antiserum labeled a protein of approximately 36 kDa. Electron microscopic examination of ultrathin sections from infected CT tissue revealed the presence of pinwheel inclusions typical of potyvirus (type 1) infection in the cytoplasm of the cell. CT seedlings were susceptible to mechanical inoculation with the ZYMV isolated from this species. This is the first report of CT as a natural host for ZYMV in Brazil. References: (1) A. Cogniaux. Flora Brasiliensis 6:1, 1878. (2) M. Pio Corrêa. 1926. Diccionário das plantas úteis do Brasil e das exóticas cultivadas. Vol 1. Ministério da Agricultura, Rio de Janeiro, Brazil.

scholarly journals First Report of Tobacco vein banding mosaic virus in China (Xian, Shaanxi Province) in Datura stramonium and Tobacco

Plant Disease ◽  
2000 ◽  
Vol 84 (10) ◽  
pp. 1152-1152 ◽  
Author(s):  
P. Roggero ◽  
G. P. Accotto ◽  
M. Ciuffo ◽  
R. Lenzi ◽  
C. Desbiez ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Tobacco Plant ◽  
Severe Disease ◽  
Datura Stramonium ◽  
Shaanxi Province ◽  
Chinese Isolate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chenopodium Amaranticolor ◽  
First Report ◽  
White Burley

Tobacco vein banding mosaic virus (TVBMV) has been reported in Taiwan (1), North America (Tennessee) (2), and Japan (3) and induces a severe disease of tobacco. During surveys on viruses of vegetables in China, TVBMV was isolated from a Datura stramonium weed plant in July 1998 in Shaanxi Province. It showed severe mosaic with blistering of the leaves. The plant was also infected by Cucumber mosaic virus (CMV). When sap from D. stramonium was frozen, thawed, and mechanically inoculated, only TVBMV was recovered. The 3′-end of the viral genome was amplified by reverse transcription-polymerase chain reaction (RT-PCR) using primers derived from the potyviridae primers (4) and cloned in pBlueScript. The sequence of 1,630 bp (GenBank AF274315) was determined on both DNA strands and found to have approximately 94% homology with other TVBMV sequences (L 28816 from Tennessee, X77637 from Taiwan, and AB020524 from Japan). The host range of the Chinese isolate was similar to that reported for the U.S. isolate. D. stramonium, Nicotiana benthamiana, N. clevelandii, N. glutinosa, N. tabacum Samsun, White Burley type and Xanthi, Lycopersicon esculentum cv. Marmande, and Petunia hybrida were systemically infected. A local infection developed in N. rustica, Chenopodium amaranticolor, C. quinoa, and Ocimum basilicum. The Chinese isolate did not infect Capsicum annuum cv. Quadrato d'Asti, Solanum melongena, or several Cucurbitaceae and Leguminosae species. Myzus persicae transmitted the Chinese TVBMV in a non-persistent mode from both D. stramonium and tobacco to the same plants and to tomato. No seed transmission occurred in experimentally infected D. stramonium (20 seedlings), tobacco White Burley type (200 seedlings), and tomato cv. Marmande (100 seedlings). The virus was found in the roots of D. stramonium and tobacco. Since the virus was not seed-transmissible, overwintering rootstocks may provide sites for winter survival of the virus. An antiserum was produced against the virus and an enzyme-linked immunosorbent assay survey was carried out in solanaceous crops including D. stramonium collected in July 1999 in Shaanxi, Shanxi, Henan, and Hebei provinces and Beijing surroundings. TVBMV was found only in the same field as in 1998 in four D. stramonium plants in association with CMV and in a tobacco plant 200 m from D. stramonium. TVBMV was not found in the closest tomato crops, where infection of CMV was severe. This is the first report of TVBMV in China, and Xian is the most northern location in which this virus has been found. References: (1) J. K. Chiang et al. Bull. Tobacco Res. Inst. 32:39, 1990. (2) B. B. Reddick et al. Plant Dis. 76:856, 1992. (3) H. Tochihara. Rev. Plant Prot. Res. 13:122, 1980. (4) A. Gibbs and A. Mackenzie. J. Virol. Meth. 63:9, 1997.

scholarly journals Cucumber mosaic virus pada Tanaman Lada di Yogyakarta dan Bangka Belitung

2019 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Emerensiana - Uge ◽  
Sri Sulandari ◽  
Sedyo - Hartono ◽  
Susamto - Somowiyarjo
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Disease Incidence ◽  
Aphis Gossypii ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Chenopodium Amaranticolor ◽  
Virus Particles ◽  
Three Samples ◽  
Long Time

Cucumber mosaic virus on Black Pepper in Yogyakarta and Bangka BelitungPepper  (Piper  nigrum)  is  spice  crop  which  has  been  cultivated  a  long  time  ago  in  Indonesia. Stunting is one of disease on pepper caused by cucumber mosaic virus (CMV). The research aimed to diagnose the biological, morphological and nucleaic acid characters of CMV on pepper in Yogyakarta and Bangka Belitung. CMV infection on pepper in both area (Putat dan Kleben village, Yogyakarta and Air Buluh village, Bangka Belitung) showed typical symptoms such as mosaic, narrow leaves and stunting. The disease incidence and disease severity of stunting disease are varies. The virus able to transmitted by cutting, grafting and mechanically on Nicotiana tabacum and Chenopodium amaranticolor. However, it was unable to transmitted mechanically on pepper and by Aphis gossypii. The virus particles were isometric with diameter size 28-30 nm. RT-PCR using coat protein partial gene primer successfully amplified a DNA with size ± 500 bp from all three samples. The homology of nucleotide between three isolates was 98-97%, while the highest homology of those three strains CMV  from Yogyakarta and Bangka Belitung was 98% against strains from China in Brassica chinensis. Three strains CMV from pepper were in the same group, and separated from CMV pepper lines from Indonesia and other CMV isolates. 

scholarly journals First Report of Sugarcane mosaic virus Infecting Maize in Poland

Plant Disease ◽  
2009 ◽  
Vol 93 (10) ◽  
pp. 1078-1078 ◽  
Author(s):  
K. Trzmiel
Keyword(s):  
Electron Microscopy ◽  
Mosaic Virus ◽  
Sugarcane Mosaic Virus ◽  
Maize Dwarf Mosaic Virus ◽  
Cdna Clones ◽  
Rt Pcr ◽  
Crop Acreage ◽  
Three Samples ◽  
Two Samples ◽  
Maize Plants

Maize (Zea mays) has become an important crop in Poland with a constant increase in crop acreage since the 1990s. In 2007, maize plants with characteristic leaf mosaic were observed in two locations in the Wielkopolska Region near Poznań and Krotoszyn. Ninety-two samples from plants showing leaf mosaic, some leaf discoloration, stunting, or no symptoms were collected and tested for Maize dwarf mosaic virus (MDMV) and Sugarcane mosaic virus (SCMV) by double-antibody sandwich (DAS)-ELISA (Bioreba, Basel, Switzerland). SCMV was detected only in three samples with distinct leave mosaic symptoms. Electron microscopy of leaf extracts revealed numerous potyvirus-like particles. Immuno-specific electron microscopy (ISEM) with the SCMV antiserum gave positive results for all three samples. Each virus isolate was propagated by mechanical inoculation on five varieties of dent maize and three varieties of sweet maize, cockspur grass (Echinochloa crus-galli (L.) Beauv.), crab grass (Digitaria sanguinalis (L.) Scop.), and green bristle-grass (Setaria viridis (L.) P.B.). Leaf mosaic appeared 4 to 5 days postinoculation. ELISA detected all three isolates in the symptomless hosts of oat (Avena sativa L.), wheat (Triticum aestivum L.), and triticale (Triticale). The three isolates induced local leaf necrosis on sorghum (Sorghum vulgare L.), in which the virus occurred in low concentrations as determined by ELISA so infections of sorghum plants were confirmed by reverse transcription (RT)-PCR) with primers PS/PSC (1). Barley (Hordeum vulgare L.), true millet (Panicum miliaceum L.), and wind grass (Agrostis spica-venti (L.) P.B.) were not susceptible (2). Using the total RNA extracted with the RNeasy Mini Kit (Qiagen, Hilden, Germany) from leaves of inoculated maize plants, a one step RT-PCR (Qiagen) amplified a ~800-bp cDNA fragment of the coat protein gene with SCMV-specific primers PS/PSC (1). Six cDNA clones were sequenced for each isolate. Nucleotide sequences of the 823-bp cDNA clones of isolates SCMV-P1 and P2 (GenBank Accession Nos. EU761241 and EU761242, respectively) were 99% identical and each was 92% identical to the sequence of SCMV-P3 (FJ376609). The clones of SCMV-P1 and SCMV-P2 shared 99, 98, 90, and 87% nucleotide sequence identity with two German SCMV isolates (X981697 and X98168), a Spanish isolate (AM110759), the UT6 isolate from Thailand (AY630923), and the Nancheng isolate (EU346720) from China, respectively. The SCMV-P3 sequence was 98, 94, 92, 89, and 92% identical to the Mx isolate from Mexico (AY195610), a Bulgarian SCMV isolate (AJ006201), the German Seehausen (X98166) and Borsdorf (X98167) isolates, the SC-UD1 (DQ647661), the KL – Co86032 (DQ866744) isolates from Thailand and India, and the Chinese Nanchang (EU346720) and Pengze2 (EU346718) isolates, respectively. In 2008, SCMV was again detected by ELISA in mixed infections with MDMV in samples from the Wielkopolska Region, but only sporadically, and the virus is considered not to be important economically in maize production in Poland. References: (1) J. X. Jiang and X. P. Zhou. Arch. Virol. 147:2437, 2002. (2) D. M. Persley. Page: 1204 in: Viruses of Plants. Descriptions and Lists from the VIDE Database. CAB International, Wallingford, UK, 1996.

scholarly journals Reação de acessos de Lycopersicon spp. a um isolado de Potato Virus Y (PVYº) de tomateiro

Bragantia ◽  
2008 ◽  
Vol 67 (2) ◽  
pp. 391-399 ◽  
Author(s):  
Silvia Regina Luz Palazzo ◽  
Addolorata Colariccio ◽  
Arlete Marchi Tavares de Melo
Keyword(s):  
Mosaic Virus ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Tomato Spot Wilt Virus ◽  
Datura Stramonium ◽  
Yellow Mosaic Virus ◽  
Tomato Mosaic Virus ◽  
Chenopodium Amaranticolor ◽  
Chlorotic Spot ◽  
Das Elisa

Amostras de tomateiro (Lycopersicon esculentum Mill) ‘Alambra’ coletadas nas regiões produtoras de Elias Fausto, Monte-Mor e Mogi-Guaçú (SP), com sintomas de amarelecimento foliar generalizado semelhante aqueles causados por vírus foram submetidas à identificação do agente causal, por testes biológicos de transmissão mecânica, pela determinação do círculo de hospedeiras, empregando-se plantas indicadoras e diferenciadoras pertencentes às famílias Chenopodiaceae e Solanaceae pela identificação sorológica por PTA-ELISA do Potato virus Y (PVY), Pepper yellow mosaic virus (PepYMV), Cucumber mosaic virus (CMV), Tomato mosaic virus (ToMV) e DAS-ELISA com antissoros policlonais para as espécies Tomato spot wilt vírus (TSWV), Tomato chlorotic spot virus (TCSV), Groundnut ringspot virus (GRSV), Chrysanthemum stem necrosis virus (CSNV) e anticorpos monoclonais para as estirpes do PVY comum (PVYº), PVY necrótico (PVY N) e PVY clorótico (PVY C). Das amostras coletadas 19 reagiram positivamente, com o PVY em PTA-ELISA e PVYºem DAS-ELISA. Plantas de Chenopodium amaranticolor reagiram com sintoma local e plantas de Nicotiana glutinosa, N. tabacum ‘WB’, N. sylvestris, N.debneyi, N. tabacum ‘Sansun’ reagiram com sintomas de mosaico sistêmico; tomateiros ‘Alambra’ manifestaram sintomas de mosaico-amarelo. Plantas de Datura stramonium, D. metel e C. annuum ‘Magda’ não foram infectadas. A ausência de sintomas em C.annuum ‘Magda’, identificou a presença do PVY patotipo 1 (PVYº1), nas 19 amostras. Como foi identificada a mesma espécie de vírus nas amostras, optou-se pela inoculação do isolado de tomate ‘Alambra’ de Elias Fausto nos dezenove acessos de Lycopersicon spp. do Banco Ativo de Germoplasma (BAG)- IAC. O delineamento dos experimentos foi inteiramente casualizado. A reação dos acessos foi avaliada pela manifestação dos sintomas, pelos resultados positivos ou negativos após testes de retro-inoculação e PTA-ELISA, pela análise do χ2 utilizando-se a proporção de plantas sintomáticas e assintomáticas, com resultados positivos ou que não manifestaram sintomas e reagiram negativamente, em PTA-ELISA, constituindo, portanto fontes potenciais de genes de resistência para o PVY em tomate.

scholarly journals Maize resistance to Sugarcane mosaic virus

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 542-544
Author(s):  
R. Pokorný ◽  
M. Porubová
Keyword(s):  
Mosaic Virus ◽  
Systemic Infection ◽  
Sugarcane Mosaic Virus ◽  
Resistant Line ◽  
Mechanisms Of Resistance ◽  
Long Distance ◽  
Maize Hybrids ◽  
Resistant Lines ◽  
Maize Resistance ◽  
Long Distance Movement

Under greenhouse conditions 12 maize hybrids derived from crosses of four resistant lines with several lines of different level of susceptibility were evaluated for resistance to Czech isolate of Sugarcane mosaic virus (SCMV). These hybrids were not fully resistant to isolate of SCMV, but the symptoms on their newly growing leaves usually developed 1 to 3 weeks later in comparison with particular susceptible line, the course of infection was significantly slower and rate of infection lower. As for mechanisms of resistance, the presence of SCMV was detected by ELISA in inoculated leaves both of resistant and susceptible lines, but virus was detected 7 days later in resistant line. Systemic infection developed only in susceptible lines. These results indicate restriction of viral long distance movement in the resistant line.

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