scholarly journals Genetic Variability Among Maize Inbred Lines for Resistance to the High Plains Virus-Wheat Streak Mosaic Virus Complex

Plant Disease ◽  
1997 ◽  
Vol 81 (2) ◽  
pp. 195-198 ◽  
Author(s):  
A. Marçon ◽  
S. M. Kaeppler ◽  
S. G. Jensen
Keyword(s):  
Genetic Variability ◽  
Mosaic Virus ◽  
Mixed Infection ◽  
Severe Disease ◽  
Virus Titer ◽  
Inbred Lines ◽  
Wheat Streak Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Plains ◽  
Maize Inbred Lines

High plains virus (HPV) is a pathogen that causes a severe disease, especially in susceptible corn genotypes. The virus is transmitted by the eriophyid mite Aceria tosichella, which also transmits wheat streak mosaic virus (WSMV). This often results in a mixed infection by these two viruses. Genetic variability for resistance to the HPV exists among maize inbred lines but has not been characterized. The disease reaction of 30 maize inbred lines to the mixed infection and to WSMV alone was characterized in this study. Evaluation was based on symptom expression and virus titer (HPV and WSMV), as measured by enzyme-linked immunosorbent assay. All lines showed some HPV symptoms, which ranged from a few visible spots to rapid plant death. HPV-resistant inbreds include B73 and B14. Susceptible inbreds include W64A, Wf9, H100, N213, N215, and N194. Five of the six inbreds most severely affected by HPV also had a high WSMV titer, although they displayed few symptoms when inoculated with WSMV alone. Inbred N194 was one of the genotypes most susceptible to HPV, but it showed no detectable WSMV titer. Seedlings of highly susceptible genotypes often died within 2 weeks of infection.

scholarly journals Mixed Infection of Hard Red Winter Wheat with High Plains Virus and Wheat Streak Mosaic Virus from Wheat Curl Mites in Nebraska

Plant Disease ◽  
1998 ◽  
Vol 82 (3) ◽  
pp. 311-315 ◽  
Author(s):  
T. Mahmood ◽  
G. L. Hein ◽  
S. G. Jensen
Keyword(s):  
Western Blot ◽  
Mosaic Virus ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Protein A ◽  
Wheat Streak Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Plains ◽  
Mixed Infections ◽  
Wheat Curl Mite

A new disease of wheat and corn caused by the High Plains virus (HPV) has been observed in the High Plains region of western United States. HPV is transmitted by the wheat curl mite, Aceria tosichella, which is also the vector of wheat streak mosaic virus (WSMV). In the field it is extremely difficult to visually differentiate plants infected with WSMV from those with HPV. An indirect protein-A sandwich enzyme-linked immunosorbent assay (PAS-ELISA) and Western blot analysis were used to identify WSMV and HPV. Samples of wheat curl mites were collected from arbitrarily chosen sites from commercial wheat plantings in 1995 and 1996 and used to infest caged wheat plants. After 3 weeks, leaf samples were harvested and assayed. Both Western blot analysis and PAS-ELISA were effective at identifying samples positive for WSMV and HPV, both alone and in mixed infections. Western blot results showed that over the 2 years, 65% of the samples were positive for WSMV, 46% were positive for HPV, and mixed infections were found in 40% of the samples. HPV presence was verified with similar results from field collected plant samples. These levels of virus indicate an unexpectedly high incidence of HPV in wheat curl mite populations in Nebraska.

scholarly journals Incidence of viruses infecting pepper in Thailand

2019 ◽  
Vol 10 (1) ◽  
pp. 184-193
Author(s):  
Amisa Laprom ◽  
Somrudee Nilthong ◽  
Ekachai Chukeatirote
Keyword(s):  
Mosaic Virus ◽  
Mixed Infection ◽  
Tomato Spotted Wilt Virus ◽  
Potato Virus Y ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mixed Infections ◽  
The North ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Chilli Veinal Mottle Virus

AbstractThis study was conducted to determine the incidence, diversity and distribution of viruses infecting pepper (Capsicum spp.) in the central, northern and northeastern parts of Thailand. During a survey in 2016 - 2019, a total of 2,149 leaf samples from symptomatic and asymptomatic peppers were collected randomly from farmer’s fields, and preliminary tested by an enzyme-linked immunosorbent assay (ELISA) using 7 antibodies specific for cucumber mosaic virus (CMV), chilli veinal mottle virus (ChiVMV), tomato necrotic ringspot virus (TNRV), tobacco mosaic virus (TMV), potato virus Y (PVY), tomato spotted wilt virus (TSWV), and begomoviruses. Our data revealed that the incidence of the viruses infecting pepper in Thailand was high, accounting for nearly 70% (1,482 infected samples). The highest viral incidence was found in the central part (96%), followed by the north (74.4%) and the northeastern (52.8%), respectively. Begomoviruses, CMV, ChiVMV, and TNRV were detected in the samples at varying rates, whereas PVY, TMV, and TSWV were not detected. Of these, the most frequently found virus was Begomoviruses accounting for nearly 33%, with the highest rate (ca. 82%) in the central Provinces of Thailand. In addition, of the 1,482 infected samples, mixed infections among the four viruses were also found in 616 samples (ca. 42%), and CMV + ChiVMV (approximately 11%) was the most common mixed infection. This is the first report describing an occurrence of viruses in pepper of Thailand, and the results obtained have revealed that viruses infecting pepper are widespread, which may pose a threat to pepper production in Thailand.

scholarly journals Complete Deletion of Wheat Streak Mosaic Virus HC-Pro: a Null Mutant Is Viable for Systemic Infection

2005 ◽  
Vol 79 (18) ◽  
pp. 12077-12080 ◽  
Author(s):  
Drake C. Stenger ◽  
Roy French ◽  
Frederick E. Gildow
Keyword(s):  
Mosaic Virus ◽  
Virus Titer ◽  
Systemic Infection ◽  
In Vitro Transcription ◽  
Wheat Streak Mosaic Virus ◽  
Null Mutant ◽  
Reverse Transcription Pcr ◽  
Virion Formation ◽  
Insertion Mutations

ABSTRACT A Wheat streak mosaic virus (WSMV) genome lacking HC-Pro was constructed and confirmed by reverse transcription-PCR to systemically infect wheat, oat, and corn. Coupled in vitro transcription/translation reactions indicated that WSMV P1 proteinase cleaved the polyprotein at the P1/P3 junction of the HC-Pro null mutant. The WSMV HC-Pro null mutant was competent for virion formation, but the virus titer was reduced 4.5-fold relative to that of the wild type. Collectively, these results indicate that WSMV HC-Pro is dispensable for replication and movement, two essential processes that are disrupted by point and small-insertion mutations introduced into potyvirus HC-Pro.

scholarly journals Loci Controlling Resistance to High Plains Virus and Wheat Streak Mosaic Virus in a B73 × Mo17 Population of Maize

Crop Science ◽  
1999 ◽  
Vol 39 (4) ◽  
pp. 1171-1177 ◽  
Author(s):  
A. Marçcon ◽  
S. M. Kaeppler ◽  
S. G. Jensen ◽  
L. Senior ◽  
C. Stuber
Keyword(s):  
Mosaic Virus ◽  
Wheat Streak Mosaic Virus ◽  
High Plains

scholarly journals Occurrence and Yield Effects of Wheat Infected with Triticum mosaic virus in Kansas

Plant Disease ◽  
2011 ◽  
Vol 95 (2) ◽  
pp. 183-188 ◽  
Author(s):  
Dallas L. Seifers ◽  
T. J. Martin ◽  
John P. Fellers
Keyword(s):  
Triticum Aestivum ◽  
Mosaic Virus ◽  
Great Plains ◽  
The United States ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Plains ◽  
The Third ◽  
Volume Weight ◽  
Significant Yield ◽  
Second Year

Triticum mosaic virus (TriMV) infects wheat (Triticum aestivum) in the Great Plains region of the United States. This study determined the occurrence of TriMV at three locations over 3 years and yield effects of wheat mechanically infected with TriMV. Wheat infection with TriMV, Wheat streak mosaic virus (WSMV), and the High Plains virus (HPV) was verified using enzyme-linked immunosorbent assay. Both wheat singly infected with TriMV and doubly infected with TriMV and WSMV occurred at three, two, and one locations in 2007, 2008, and 2009, respectively. Wheat singly infected with HPV occurred at one and two locations in 2008 and 2009, respectively. Wheat doubly infected with WSMV and HPV occurred at one location in 2008 and 2009. Infection with TriMV declined at two locations each year and, at the third location, it increased the second year and was not detected the third year. WSMV infection increased, except for a decline the third year at one location. In contrast to 3.0% infection of wheat with TriMV and WSMV at one location, 85% of the wheat 1.6 km from that site was infected with TriMV and WSMV in 2009. Infection of wheat with TriMV caused significant yield and volume weight reductions in Danby, RonL, and Jagalene but not KS96HW10-3 wheat.

scholarly journals Peroxidase Activity in Maize Inbred Lines Resistant or Susceptible to Maize Dwarf Mosaic Virus

2003 ◽  
Vol 2 (1) ◽  
pp. 1-8 ◽  
Author(s):  
I.R.P. Souza ◽  
E. Oliveira ◽  
M.A. Peres ◽  
A.C. Oliveira ◽  
A.Á.C. Purcino
Keyword(s):  
Mosaic Virus ◽  
Peroxidase Activity ◽  
Inbred Lines ◽  
Maize Dwarf Mosaic Virus ◽  
Maize Inbred Lines
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