scholarly journals First Report of Leaf and Stem Rust Caused by Puccinia sherardiana on Sphaeralcea grossulariaefolia in North America and S. munroana in Wyoming

Plant Disease ◽  
1998 ◽  
Vol 82 (7) ◽  
pp. 831-831
Author(s):  
S. C. Briere ◽  
G. D. Franc

Diseased samples of globe mallow, Sphaeralcea grossulariaefolia and S. munroana, were submitted by an ornamental seed producer in Wyoming to our Extension Plant Pathology Laboratory in July 1997. Dark brown, amphigenous telia surrounded by chlorotic halos were present on both foliage and stems. Mean teliospore dimensions observed were 30.8 × 44.8 μm. The teliospores germinated readily on water agar at 20°C and formed basidiospores within 24 h. Aecia and uredinia were not found. Based on characteristics mentioned above, this fungus was identified as Puccinia sherardiana Körn (1). This microcyclic rust was previously described on 12 other Sphaeralcea spp. plus other plant species in the Malvaceae family (1,2). Stem and foliar symptoms were reproduced in a greenhouse on 8-week-old plants of S. grossulariaefolia and S. munroana. These plants were inoculated with teliospores removed from the original diseased plant material. Immediately after inoculation, plants were misted and placed in plastic bags and incubated for 36 h at 100% relative humidity and 20°C. Plants continued growth with natural lighting and with day and night temperatures of 20 and 15°C, respectively. Symptoms developed within 12 days with initial telia rupturing the host epidermis 13 days after inoculation. Telia were examined microscopically to complete Koch's postulates. References: (1) J. C. Arthur. Manual of Plant Rusts in United States and Canada. Hafner Pub., 1962. (2) D. F. Farr et al. Fungi on Plants and Plant Products in the United States. American Phytopatholical Society, St. Paul, MN, 1989.

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Kevin Lanza ◽  
Melody Alcazar ◽  
Deanna M. Hoelscher ◽  
Harold W. Kohl

Abstract Background Latinx children in the United States are at high risk for nature-deficit disorder, heat-related illness, and physical inactivity. We developed the Green Schoolyards Project to investigate how green features—trees, gardens, and nature trails—in school parks impact heat index (i.e., air temperature and relative humidity) within parks, and physical activity levels and socioemotional well-being of these children. Herein, we present novel methods for a) observing children’s interaction with green features and b) measuring heat index and children’s behaviors in a natural setting, and a selection of baseline results. Methods During two September weeks (high temperature) and one November week (moderate temperature) in 2019, we examined three joint-use elementary school parks in Central Texas, United States, serving predominantly low-income Latinx families. To develop thermal profiles for each park, we installed 10 air temperature/relative humidity sensors per park, selecting sites based on land cover, land use, and even spatial coverage. We measured green features within a geographic information system. In a cross-sectional study, we used an adapted version of System for Observing Play and Recreation in Communities (SOPARC) to assess children’s physical activity levels and interactions with green features. In a cohort study, we equipped 30 3rd and 30 4th grade students per school during recess with accelerometers and Global Positioning System devices, and surveyed these students regarding their connection to nature. Baseline analyses included inverse distance weighting for thermal profiles and summing observed counts of children interacting with trees. Results In September 2019, average daily heat index ranged 2.0 °F among park sites, and maximum daily heat index ranged from 103.4 °F (air temperature = 33.8 °C; relative humidity = 55.2%) under tree canopy to 114.1 °F (air temperature = 37.9 °C; relative humidity = 45.2%) on an unshaded playground. 10.8% more girls and 25.4% more boys interacted with trees in September than in November. Conclusions We found extreme heat conditions at select sites within parks, and children positioning themselves under trees during periods of high heat index. These methods can be used by public health researchers and practitioners to inform the redesign of greenspaces in the face of climate change and health inequities.


Plant Disease ◽  
2014 ◽  
Vol 98 (5) ◽  
pp. 696-696 ◽  
Author(s):  
J. A. Crouch ◽  
M. P. Ko ◽  
J. M. McKemy

Downy mildew of impatiens (Impatiens walleriana Hook.f.) was first reported from the continental United States in 2004. In 2011 to 2012, severe and widespread outbreaks were documented across the United States mainland, resulting in considerable economic losses. On May 5, 2013, downy mildew disease symptoms were observed from I. walleriana ‘Super Elfin’ at a retail nursery in Mililani, on the Hawai'ian island of Oahu. Throughout May and June 2013, additional sightings of the disease were documented from the islands of Oahu, Kauai, Maui, and Hawai'i from nurseries, home gardens, and botanical park and landscape plantings. Symptoms of infected plants initially showed downward leaf curl, followed by a stippled chlorotic appearance on the adaxial leaf surfaces. Abaxial leaf surfaces were covered with a layer of white mycelia. Affected plants exhibited defoliation, flower drop, and stem rot as the disease progressed. Based on morphological and molecular data, the organism was identified as Plasmopara obducens (J. Schröt.) J. Schröt. Microscopic observation disclosed coenocytic mycelium and hyaline, thin-walled, tree-like (monopodial branches), straight, 94.0 to 300.0 × 3.2 to 10.8 μm sporangiophores. Ovoid, hyaline sporangia measuring 11.0 to 14.6 × 12.2 to 16.2 (average 13.2 × 14.7) μm were borne on sterigma tips of rigid branchlets (8.0 to 15.0 μm) at right angle to the main axis of the sporangiophores (1,3). Molecular identification of the pathogen was conducted by removing hyphae from the surface of three heavily infected leaves using sterile tweezers, then extracting DNA using the QIAGEN Plant DNA kit (QIAGEN, Gaithersburg, MD). The nuclear rDNA internal transcribed spacer was sequenced from each of the three samples bidirectionally from Illustra EXOStar (GE Healthcare, Piscataway, NJ) purified amplicon generated from primers ITS1-O and LR-0R (4). Resultant sequences (GenBank KF366378 to 80) shared 99 to 100% nucleotide identity with P. obducens accession DQ665666 (4). A voucher specimen (BPI892676) was deposited in the U.S. National Fungus Collections, Beltsville, MD. Pathogenicity tests were performed by spraying 6-week-old impatiens plants (I. walleriana var. Super Elfin) grown singly in 4-inch pots with a suspension of 1 × 104 P. obducens sporangia/ml until runoff using a handheld atomizer. Control plants were sprayed with distilled water. The plants were kept in high humidity by covering with black plastic bags for 48 h at 20°C, and then maintained in the greenhouse (night/day temperature of 20/24°C). The first symptoms (downward curling and chlorotic stippling of leaves) and sporulation of the pathogen on under-leaf surfaces of the inoculated plants appeared at 10 days and 21 days after inoculation, respectively. Control plants remained healthy. Morphological features and measurements matched those of the original inoculum, thus fulfilling Koch's postulates. To our knowledge, this is the first report of downy mildew on I. walleriana in Hawai'i (2). The disease appears to be widespread throughout the islands and is likely to cause considerable losses in Hawai'ian landscapes and production settings. References: (1) O. Constantinescu. Mycologia 83:473, 1991. (2) D. F. Farr and A. Y. Rossman. Systematic Mycology and Microbiology Laboratory, ARS, USDA. Retrieved from http://nt.ars-grin.gov/fungaldatabases/ July 16, 2013. (3) P. A. Saccardo. Syllogue Fungorum 7:242, 1888. (4) M. Thines. Fungal Genet Biol 44:199, 2007.


Plant Disease ◽  
2012 ◽  
Vol 96 (2) ◽  
pp. 287-287
Author(s):  
K. S. Han ◽  
J. H. Park ◽  
S. E. Cho ◽  
H. D. Shin

Pachysandra terminalis Siebold & Zucc., known as Japanese pachysandra, is a creeping evergreen perennial belonging to the family Buxaceae. In April 2011, hundreds of plants showing symptoms of leaf blight and stem canker with nearly 100% incidence were found in a private garden in Suwon, Korea. Plants with the same symptoms were found in Seoul in May and Hongcheon in August. Affected leaves contained tan-to-yellow brown blotches. Stem and stolon cankers first appeared as water soaked and developed into necrotic lesions. Sporodochia were solitary, erumpent, circular, 50 to 150 μm in diameter, salmon-colored, pink-orange when wet, and with or without setae. Setae were hyaline, acicular, 60 to 100 μm long, and had a base that was 4 to 6 μm wide. Conidiophores were in a dense fascicle, not branched, hyaline, aseptate or uniseptate, and 8 to 20 × 2 to 3.5 μm. Conidia were long, ellipsoid to cylindric, fusiform, rounded at the apex, subtruncate at the base, straight to slightly bent, guttulate, hyaline, aseptate, 11 to 26 × 2.5 to 4.0 μm. A single-conidial isolate formed cream-colored colonies that turned into salmon-colored colonies on potato dextrose agar (PDA). Morphological and cultural characteristics of the fungus were consistent with previous reports of Pseudonectria pachysandricola B.O. Dodge (1,3,4). Voucher specimens were housed at Korea University (KUS). Two isolates, KACC46110 (ex KUS-F25663) and KACC46111 (ex KUS-F25683), were accessioned in the Korean Agricultural Culture Collection. Fungal DNA was extracted with DNeasy Plant Mini DNA Extraction Kits (Qiagen Inc., Valencia, CA). The complete internal transcribed spacer (ITS) region of rDNA was amplified with the primers ITS1/ITS4 and sequenced using ABI Prism 337 automatic DNA sequencer (Applied Biosystems, Foster, CA). The resulting sequence of 487 bp was deposited in GenBank (Accession No. JN797821). This showed 100% similarity with a sequence of P. pachysandricola from the United States (HQ897807). Isolate KACC46110 was used in pathogenicity tests. Inoculum was prepared by harvesting conidia from 2-week-old cultures on PDA. Ten young leaves wounded with needles were sprayed with conidial suspensions (~1 × 106 conidia/ml). Ten young leaves that served as the control were treated with sterile distilled water. Plants were covered with plastic bags to maintain a relative humidity of 100% at 25 ± 2°C for 24 h. Typical symptoms of brown spots appeared on the inoculated leaves 4 days after inoculation and were identical to the ones observed in the field. P. pachysandricola was reisolated from 10 symptomatic leaf tissues, confirming Koch's postulates. No symptoms were observed on control plants. Previously, the disease was reported in the United States, Britain, Japan, and the Czech Republic (2,3), but not in Korea. To our knowledge, this is the first report of P. pachysandricola on Pachysandra terminalis in Korea. Since this plant is popular and widely planted in Korea, this disease could cause significant damage to nurseries and the landscape. References: (1) B. O. Dodge. Mycologia 36:532, 1944. (2) D. F. Farr and A. Y. Rossman. Fungal Databases. Systematic Mycology and Microbiology Laboratory, ARS, USDA. Retrieved from http://nt.ars-grin.gov/fungaldatabases/ , September 24, 2011. (3) I. Safrankova. Plant Prot. Sci. 43:10, 2007. (4) W. A. Sinclair and H. H. Lyon. Disease of Trees and Shrubs. 2nd ed. Cornell University Press, Ithaca, NY, 2005.


2018 ◽  
Vol 33 (1) ◽  
pp. 301-315 ◽  
Author(s):  
Wesley G. Page ◽  
Natalie S. Wagenbrenner ◽  
Bret W. Butler ◽  
Jason M. Forthofer ◽  
Chris Gibson

Abstract Wildland fire managers in the United States currently utilize the gridded forecasts from the National Digital Forecast Database (NDFD) to make fire behavior predictions across complex landscapes during large wildfires. However, little is known about the NDFDs performance in remote locations with complex topography for weather variables important for fire behavior prediction, including air temperature, relative humidity, and wind speed. In this study NDFD forecasts for calendar year 2015 were evaluated in fire-prone locations across the conterminous United States during periods with the potential for active fire spread using the model performance statistics of root-mean-square error (RMSE), mean fractional bias (MFB), and mean bias error (MBE). Results indicated that NDFD forecasts of air temperature and relative humidity performed well with RMSEs of about 2°C and 10%–11%, respectively. However, wind speed was increasingly underpredicted when observed wind speeds exceeded about 4 m s−1, with MFB and MBE values of approximately −15% and −0.5 m s−1, respectively. The importance of accurate wind speed forecasts in terms of fire behavior prediction was confirmed, and the forecast accuracies needed to achieve “good” surface head fire rate-of-spread predictions were estimated as ±20%–30% of the observed wind speed. Weather station location, the specific forecast office, and terrain complexity had the largest impacts on wind speed forecast error, although the relatively low variance explained by the model (~37%) suggests that other variables are likely to be important. Based on these results it is suggested that wildland fire managers should use caution when utilizing the NDFD wind speed forecasts if high wind speed events are anticipated.


2021 ◽  
Author(s):  
Kevin Lanza ◽  
Melody Alcazar ◽  
Deanna M. Hoelscher ◽  
III Harold W. Kohl

Abstract Background: Latinx children in the United States are at high risk for nature-deficit disorder, heat-related illness, and physical inactivity. We developed the Green Schoolyards Project to investigate how green features—trees, gardens, and nature trails—in school parks impact heat index (i.e., air temperature and relative humidity) within parks, and physical activity levels and socioemotional well-being of these children. Herein, we present novel methods for a) observing children’s interaction with green features and b) measuring heat index and children’s behaviors in a natural setting, and a selection of baseline results.Methods: During two September weeks (high temperature) and one November week (moderate temperature) in 2019, we examined three joint-use elementary school parks in Central Texas, United States, serving predominantly low-income Latinx families. To develop thermal profiles for each park, we installed 10 air temperature/relative humidity sensors per park, selecting sites based on land cover, land use, and even spatial coverage. We measured green features within a geographic information system. In a cross-sectional study, we used an adapted version of System for Observing Play and Recreation in Communities (SOPARC) to assess children’s physical activity levels and interactions with green features. In a cohort study, we equipped 30 3rd and 30 4th grade students per school during recess with accelerometers and Global Positioning System devices, and surveyed these students regarding their connection to nature. Baseline analyses included inverse distance weighting for thermal profiles and summing observed counts of children interacting with trees.Results: In September 2019, average daily heat index ranged 2.0°F among park sites, and maximum daily heat index ranged from 103.4°F (air temperature = 33.8°C; relative humidity = 55.2%) under tree canopy to 114.1°F (air temperature = 37.9°C; relative humidity = 45.2%) on an unshaded playground. 10.8% more girls and 25.4% more boys interacted with trees in September than in November.Conclusions: We found extreme heat conditions at select sites within parks, and children positioning themselves under trees during periods of high heat index. These methods can be used by public health researchers and practitioners to inform the redesign of greenspaces in the face of climate change and health inequities.


HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 884A-884
Author(s):  
Albert Sutherland* ◽  
Mike Schnelle ◽  
Derek Arndt

The American Horticulture Society (AHS) Heat Zone categories have been developed to categorize ornamental plant adaptability to different air temperature climates. These zones, like the Plant Hardiness map showing plant cold hardiness zones within the United States, are primarily north to south zones. Within the Great Plains region of the United States, the AHS Heat Zone categories provide a basic level of plant adaptability to air temperature, but do not account for plant reaction to variations in wind, relative humidity or sunlight. Daily reference evapotranspiration provides a single number that responds to variations in air temperature, wind, relative humidity and sunlight. In Oklahoma, the Oklahoma Mesonet provides a uniform statewide network of weather monitor towers that can be used to accurately calculate both short and tall American Society of Civil Engineers (ASCE) reference evapotranspiration (ref ET) across the entire state. Accumulated daily ref ET values can be used to provide further refinement in categorizing ornamental plant adaptability.


2020 ◽  
Author(s):  
Kevin Lanza ◽  
Melody Alcazar ◽  
Deanna M. Hoelscher ◽  
III Harold W. Kohl

Abstract Background: Latinx children in the United States are at high risk for nature-deficit disorder, heat-related illness, and physical inactivity. We developed the Green Schoolyards Project to investigate how green features—trees, gardens, and nature trails—in school parks impact heat index (i.e., air temperature and relative humidity) within parks, and physical activity levels and socioemotional well-being of these children. Herein, we present novel methods for a) observing children’s interaction with green features and b) measuring heat index and children’s behaviors in a natural setting, and a selection of baseline results.Methods: During two September weeks (high temperature) and one November week (moderate temperature) in 2019, we examined three joint-use elementary school parks in Central Texas, United States, serving predominantly low-income Latinx families. To develop thermal profiles for each park, we installed 10 air temperature/relative humidity sensors per park, selecting sites based on land cover, land use, and even spatial coverage. We measured green features within a geographic information system. In a cross-sectional study, we used an adapted version of System for Observing Play and Recreation in Communities (SOPARC) to assess children’s physical activity levels and interactions with green features. In a cohort study, we equipped 30 3rd and 30 4th grade students per school during recess with accelerometers and Global Positioning System devices, and surveyed these students regarding their connection to nature. Baseline analyses included inverse distance weighting for thermal profiles and summing observed counts of children interacting with trees.Results: In September 2019, average daily heat index ranged 2.0°F among park sites, and maximum daily heat index ranged from 103.4°F (air temperature = 33.8°C; relative humidity = 55.2%) under tree canopy to 114.1°F (air temperature = 37.9°C; relative humidity = 45.2%) on an unshaded playground. 10.8% more girls and 25.4% more boys interacted with trees in September than in November.Conclusions: We found extreme heat conditions at select sites within parks, and children positioning themselves under trees during periods of high heat index. These methods can be used by public health researchers and practitioners to inform the redesign of greenspaces in the face of climate change and health inequities.


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