scholarly journals First Report and Characterization of Rhynchosia golden mosaic virus in Honduras

Plant Disease ◽  
2000 ◽  
Vol 84 (9) ◽  
pp. 1045-1045 ◽  
Author(s):  
J. L. Potter ◽  
M. M. Roca de Doyle ◽  
M. K. Nakhla ◽  
D. P. Maxwell
Keyword(s):  
Mosaic Virus ◽  
Sequence Comparison ◽  
Recombinant Plasmid ◽  
Yellow Mosaic Virus ◽  
Viral Dna ◽  
Specific Primers ◽  
Common Region ◽  
First Report ◽  
Golden Yellow ◽  
The Common

Rhynchosia minima was suspected to be a weed host of Bean golden yellow mosaic virus (BGYMV, previously designated Bean golden mosaic virus type II). Leaf tissue that exhibited yellow mosaic foliar symptoms characteristic of a geminivirus infection was collected in the Comayagua Valley in Honduras in July 1999. Extraction of viral DNA from the symptomatic leaves was accomplished with the DNeasy Plant Mini Kit (Qiagen Inc., Valencia, CA). Subsequent viral DNA amplification was accomplished with degenerative primers for the cp gene (AV494/AC1048) (4). The 570-bp fragment was cloned into the pGEM T-Easy vector (Promega Corp., Madison, WI) producing the recombinant plasmid pRhyb5. The viral insert was sequenced, and from this sequence, specific primers (RHc549 and RHv29) were designed to amplify the remaining part of DNA-A. The 2.1-kb-amplified polymerase chain reaction (PCR) fragment was cloned into the pGEM T-Easy vector producing the recombinant plasmid (pRhya-sp), and the viral insert was sequenced. Nucleotide sequence comparison (GAP program, Wisconsin Package Version 10.0, Genetics Computer Group, Madison, WI) of the complete 2,624-bp DNA-A (GenBank accession no. AF239671) to geminiviruses representing the major phylogenetic clusters (1) showed nucleotide identities ranging from 63 to 82%. Sequence comparisons for the common region and rep, trap, ren, and cp genes with the most closely related geminivirus, Pepper hausteco virus (PHV, X70418), gave 76, 82, 79, 81, and 82% nucleotide identities, respectively. There is a direct repeat (TATCGGT) of 7 nt 5′ (viral sense polarity) of the conserved TATA box, and this repeat is most analogous to that in PHV (1). Specific primers were designed in the complementary sense (RGBc2414, BGBc2553) from the common region DNA-A sequence and used with a degenerative viral sense primer for the DNA-B (PBC1v2039) (3) to amplify a 647-bp fragment. Sequence comparison for the common region (134 nt from the rep gene start codon toward the 3′ end) from the DNA-B sequence had 88% nt identity to the DNA-A sequence, thus indicating that this geminivirus is bipartite. These sequence analyses indicated that this geminivirus isolated from R. minima is distinct from previously described geminiviruses, and we propose the name Rhynchosia golden mosaic virus (RGMV). From rep gene sequence alignments, RGMV has an apparent genome recombination between Old and New World geminiviruses (Tomato yellow leaf curl virus and Bean dwarf mosaic virus) as previously noted for PHV (2). Our results indicate that RGMV is a distinct geminivirus from BGYMV, and, thus, additional studies are needed to establish the importance of R. minima as a reservoir for vegetable-infecting geminiviruses. This study is the first report of another virus in the PHV phylogenetic cluster and is thus of importance in the understanding of recombinant viruses and their phylogenetic relationship to other characterized geminiviruses. References: (1) J. C. Faria et al. Phytopathology 84:321, 1994. (2) M. Padidam et al. Virology 265:218, 1999. (3) M. R. Rojas et al. Plant Dis. 77:340, 1993. (4) S. Wyatt and J. K. Brown. Phytopathology 86:1288, 1996.

scholarly journals Detecting bean golden yellow mosaic virus in bean breeding lines and in the common legume weed Macroptilium lathyroides in Puerto Rico.

1969 ◽  
Vol 85 (3-4) ◽  
pp. 165-176
Author(s):  
Lydia I. Rivera-Vargas ◽  
Vilmaris Bracero-Acosta ◽  
James S. Beaver ◽  
Dan E. Purcifull ◽  
Jane E. Polston ◽  
...  
Keyword(s):  
Puerto Rico ◽  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Phaseolus Vulgaris L ◽  
Breeding Lines ◽  
Golden Yellow ◽  
Enzymelinked Immunosorbent Assay ◽  
The Common ◽  
Polymerase Chain ◽  
Macroptilium Lathyroides

Bean golden yellow mosaic virus (BGYMV) is a geminivirus transmitted by whiteflies (Genus: Bemisia). This virus causes significant fosses in common bean (Phaseolus vulgaris L.). Serological techniques such as enzymelinked immunosorbent assay (ELISA) have been widely used for detection of viruses. We evaluated existing monoclonal antibodies (3F7,2G5 and 5C5) for the detection of BGYMV isolates in bean fines in Puerto Rico. Monoclonal antibody 3F7 was the most effective in detecting the virus in tissues of line DOR 364 and susceptible cuftivars Top Crop and Quest. However, it was not effective in the detection of BGYMV in lines of DOR 303, which showed typical symptoms. Sampfes from Macroptilium lathyroides, a weed that might be a possible reservoir of the virus, were also tested for viraf infection. ELISA tests were inconclusive for detection of geminiviruses in M. lathyroides. Polymerase Chain Reaction (PCR) was also used to complement BGYMV diagnosis in M. lathyroides and in bean lines that showed symptoms but were negative for the ELfSA test. Two sets of primers, specific for Begomovirus such as BGYMV, were used in PCR experiments. Using PCR, we were able to detect the virus in the line DOR 303 and in M. lathyroides tissues.

scholarly journals DNA Analysis Confirms Macroptilium lathyroides as Alternative Host of Bean golden yellow mosaic virus

Plant Disease ◽  
2003 ◽  
Vol 87 (9) ◽  
pp. 1022-1025 ◽  
Author(s):  
V. Bracero ◽  
L. I. Rivera ◽  
J. S. Beaver
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Nucleotide Sequence Analysis ◽  
Alternative Host ◽  
Degenerate Primers ◽  
Viral Dna ◽  
Golden Yellow ◽  
Macroptilium Lathyroides

The leguminous weed Macroptilium lathyroides is considered a potential host of the Bean golden yellow mosaic virus (BGYMV; BGMV = Mesoamerican isolates). To determine if M. lathyroides could be a host for BGYMV, an infectivity cycle was established between this weed and Phaseolus vulgaris. Virus transmission was carried out using the whitefly, Bemisia argentifolli, as a vector. Inoculated plants of both species were examined for symptoms such as mosaic, stunting, and leaf distortion. P. vulgaris and M. lathyroides showed golden yellow mosaic symptoms during all infectivity cycle stages. Symptomatic plants of both species were tested for BGYMV using polymerase chain reaction (PCR) and nucleotide sequence analysis. Two degenerate primers sets were used for PCR to detect viral DNA: PAL1v1978/PAR1c715 and PCRc2/PBL12039. PCR analysis using primers PCRc2/PBL12039 amplified viral DNA for component B from both plant species. Nucleotide sequence analysis revealed a 93% identity between the virus isolated from M. lathyroides and the Puerto Rican isolate of BGYMV. These results confirmed that M. lathyroides could serve as an alternative host of BGYMV and that an infectivity cycle of BGYMV could possibly occur between P. vulgaris and M. lathyroides in Puerto Rico.

scholarly journals Taino Tomato Mottle Virus, a New Bipartite Geminivirus from Cuba

Plant Disease ◽  
1997 ◽  
Vol 81 (9) ◽  
pp. 1095-1095 ◽  
Author(s):  
P. L. Ramos ◽  
O. Guerra ◽  
R. Peral ◽  
P. Oramas ◽  
R. G. Guevara ◽  
...  
Keyword(s):  
Amino Acid ◽  
Mosaic Virus ◽  
Nucleotide Sequences ◽  
Amino Acid Sequences ◽  
Mottle Virus ◽  
Yellow Mosaic Virus ◽  
Viral Dna ◽  
Common Region ◽  
Field Samples ◽  
Pepper Plants

Geminiviruses have become the most important virus group affecting tomatoes (Lycopersicon lycopersicum (L.) Karsten) in Cuba since they have been detected in all tomato-producing areas, causing serious losses. Recently, a whitefly-transmitted, monopartite geminivirus was detected in Cuba and identified as tomato yellow leaf curl virus-Israel (TYLCV-Is) (1). Samples collected from the main tomato-producing areas during the period 1995 to1996 were further analyzed by polymerase chain reaction (PCR) with degenerate primers (PAL1v1978 and PAR1c496) (2). Whereas in samples from most areas only TYLCV was detected, in some samples from the Havana area, two DNA fragments (approximately 1.4 and 1.1 kb) were amplified by PCR. The larger fragment was identified as part of the TYLCV-Is genome, confirming the previous report (1). The 1.1-kb fragment was cloned and its nucleotide sequence suggested that a new bipartite geminivirus was also present in those tomato samples. To clone the entire genome, tomato plants were inoculated by biolistics with DNA extract from field samples. After symptom expression, a viral DNA-enriched preparation from the inoculated tomatoes was independently digested with several restriction enzymes and the products were ligated into pZero plasmid (Invitrogen, San Diego, CA). Several clones in the 2.6-kb size range were characterized by restriction mapping and hybridization against component A and B heterologous probes. Two clones were selected as containing putative A and B components and their infectivity was tested by biolistic inoculation of tomato and pepper plants. The inoculated tomatoes developed a mild mottle in the younger leaves, whereas no symptoms were visible on the inoculated pepper plants. However, the presence of viral DNA was confirmed in both tomatoes and peppers by Southern blot hybridization analysis with A- and B-specific probes. Partial sequences of both components were obtained and their analysis showed that both components shared a 170-bases common region with a 95% identity. In addition, the nucleotide sequences of two open reading frames, one in each component (AC1 and BC1), were determined and compared with geminivirus sequences deposited in Gen-Bank. A dendogram generated with the CLUSTAL program and obtained with the AC1 and BC1 amino acid sequences, placed the new geminivirus in a cluster with tomato mottle virus (ToMoV; accession nos. L14460, L14461), Abutilon mosaic virus (AbMV; X15983, X15984), potato yellow mosaic virus (PYMV; D00940, D00941), and bean dwarf mosaic virus (BDMV; M88179, M88180). The percentages of identity obtained with the amino acid sequences were as follows. For AC1: ToMoV, 87%; PYMV, 79.5%; BDMV, 78.7%; and AbMV, 78%. For BC1 protein: BDMV, 92.8%; ToMoV, 89.1%; PYMV, 88.1%; and AbMV, 67.5%. In addition, the sequences were compared with partial nucleotide sequences (AC1, coat protein [CP], and common region) of a bipartite geminivirus affecting tomatoes in Jamaica (accession nos. U83855, U83854, and U83850). Interestingly, the common regions showed a higher percentage of identity (88%) than the CP and AC1 partial nucleotide sequences (86 and 74%, respectively). These data suggest that the virus reported here is a new geminivirus and the first bipartite geminivirus reported in Cuba. Thus, the name of Taino tomato mottle virus is proposed. (Taino refers to the name of the inhabitants of Cuba at the time of Columbus's arrival in the Caribbean). References: (1) P. L. Ramos et al. Plant Dis. 80:1208, 1996. (2) M. R. Rojas et al. Plant Dis. 77:340, 1993.

scholarly journals Screening Metagenomic Data for Viruses Using the E-Probe Diagnostic Nucleic Acid Assay

2014 ◽  
Vol 104 (10) ◽  
pp. 1125-1129 ◽  
Author(s):  
A. H. Stobbe ◽  
W. L. Schneider ◽  
P. R. Hoyt ◽  
U. Melcher
Keyword(s):  
Nucleic Acid ◽  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Metagenomic Data ◽  
Data Sets ◽  
Virus Species ◽  
Data Set ◽  
Golden Yellow ◽  
Nucleic Acid Assay ◽  
Ngs Data

Next generation sequencing (NGS) is not used commonly in diagnostics, in part due to the large amount of time and computational power needed to identify the taxonomic origin of each sequence in a NGS data set. By using the unassembled NGS data sets as the target for searches, pathogen-specific sequences, termed e-probes, could be used as queries to enable detection of specific viruses or organisms in plant sample metagenomes. This method, designated e-probe diagnostic nucleic acid assay, first tested with mock sequence databases, was tested with NGS data sets generated from plants infected with a DNA (Bean golden yellow mosaic virus, BGYMV) or an RNA (Plum pox virus, PPV) virus. In addition, the ability to detect and differentiate among strains of a single virus species, PPV, was examined by using probe sets that were specific to strains. The use of probe sets for multiple viruses determined that one sample was dually infected with BGYMV and Bean golden mosaic virus.

scholarly journals First Report of Infection of Fallopia multiflora with cucumber mosaic virus and zucchini yellow mosaic virus

2018 ◽  
Vol 100 (2) ◽  
pp. 333-333 ◽  
Author(s):  
Nam-Yeon Kim ◽  
Hyo-Jeong Lee ◽  
Mi-Ri Park ◽  
Jin-Sung Hong ◽  
Rae-Dong Jeong
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
First Report

scholarly journals Two Newly Described Begomoviruses of Macroptilium lathyroides and Common Bean

2003 ◽  
Vol 93 (7) ◽  
pp. 774-783 ◽  
Author(s):  
A. M. Idris ◽  
E. Hiebert ◽  
J. Bird ◽  
J. K. Brown
Keyword(s):  
Common Bean ◽  
Mosaic Virus ◽  
Dna Sequences ◽  
Western Hemisphere ◽  
Yellow Mosaic Virus ◽  
Nucleotide Identity ◽  
Caribbean Region ◽  
Golden Yellow ◽  
The Caribbean ◽  
Macroptilium Lathyroides

Macroptilium lathyroides, a perennial weed in the Caribbean region and Central America, is a host of Macroptilium yellow mosaic Florida virus (MaYMFV) and Macroptilium mosaic Puerto Rico virus (MaMPRV). The genomes of MaYMFV and MaMPRV were cloned from M. lathyroides and/or field-infected bean and the DNA sequences were determined. Cloned A and B components for both viruses were infectious when inoculated to M. lathyroides and common bean. Comparison of the DNA sequences for cloned A and B components with well-studied begomovirus indicated that MaMPRV (bean and M. lathyroides) and MaYMFV (M. lathyroides) are unique, previously undescribed begomo-viruses from the Western Hemisphere. Phylogenetic analysis of viral A components indicated that the closest relative of MaYMFV are members of the Bean golden yellow mosaic virus (BGYMV) group, at 76 to 78% nucleotide identity, whereas the closest relative for the A component of MaMPRV was Rhynchosia golden mosaic virus at 78% nucleotide identity. In contrast, BGYMV is the closest relative for the B component of both MaYMFV and MaMPRV, with which they share ≈68.0 and ≈72% identity, respectively. The incongruent taxonomic placement for the bipartite components for MaMPRV indicates that they did not evolve entirely along a common path. MaYMFV and MaMPRV caused distinctive symptoms in bean and M. lathyroides and were transmissible by the whitefly vector and by grafting; however, only MaYMFV was mechanically transmissible. The experimental host range for the two viruses was similar and included species within the families Fabaceae and Malvaceae, but only MaYMFV infected Malva parviflora and soybean. These results collectively indicate that MaMPRV and MaYMFV are new, previously undescribed species of the BGYMV group, a clade previously known to contain only strains and isolates of BGYMV from the Caribbean region that infect Phaseolus spp. Both MaYMFV and MaMPRV may pose an economic threat to bean production in the region.

scholarly journals First report of zucchini yellow mosaic virus in muskmelon (Cucumis melo) in Korea

2019 ◽  
Vol 101 (3) ◽  
pp. 771-771 ◽  
Author(s):  
In-Sook Cho ◽  
Bong-Nam Chung ◽  
Sun-Jung Kwon ◽  
Ju-Yeon Yoon ◽  
Gug-Seoun Choi ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Cucumis Melo ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
First Report

scholarly journals Genetic Diversity Among Geminiviruses Associated with the Weed Species Sida spp., Macroptilium lathyroides, and Wissadula amplissima from Jamaica

Plant Disease ◽  
1997 ◽  
Vol 81 (11) ◽  
pp. 1251-1258 ◽  
Author(s):  
Marcia E. Roye ◽  
Wayne A. McLaughlin ◽  
Medhat K. Nakhla ◽  
Douglas P. Maxwell
Keyword(s):  
Genetic Diversity ◽  
Amino Acid ◽  
Mosaic Virus ◽  
Amino Acid Sequences ◽  
Yellow Mosaic Virus ◽  
Weed Species ◽  
Degenerate Primers ◽  
Sequence Comparisons ◽  
The Common ◽  
Macroptilium Lathyroides

Genetic diversity among geminiviruses associated with three common weeds in Jamaica was studied using digoxigenin-labeled geminiviral DNA probes, polymerase chain reaction with degenerate primers for DNA-A and DNA-B, nucleic acid sequencing, and derived amino acid sequences. Geminiviruses with bipartite genomes were found in Sida spp., Macroptilium lathyroides, and Wissadula amplissima. The geminiviruses detected in Sida spp. and M. lathyroides were nearly identical and were both designated Sida golden mosaic geminivirus (SidGMV-JA), whereas the geminivirus in W. amplissima was sufficiently different to be designated Wissadula golden mosaic geminivirus (WGMV). Nucleotide sequence comparisons of the common regions and the N-terminal regions of the AC1 (rep) and AV1 ORFs, together with the derived amino acid sequence comparisons of the N-terminal parts of BC1 and BV1 ORFs were used to determine their similarities to other geminiviruses. SidGMV-JA was most similar to potato yellow mosaic geminivirus (PYMV). We propose that these two geminiviruses (SidGMV-JA and PYMV) define a new geminivirus cluster, the potato yellow mosaic virus (PYMV) cluster. WGMV was most similar to members of the Abutilon mosaic virus cluster but is not likely to be included in the Abutilon phylogenetic group because of the divergent sequence of the common region. These results indicate that geminiviruses infecting some weeds in Jamaica are distinct from crop-infecting geminiviruses in Jamaica and define a new geminivirus cluster.

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