scholarly journals Citrus and Coffee Strains of Xylella fastidiosa Induce Pierce's Disease in Grapevine

Plant Disease ◽  
2002 ◽  
Vol 86 (11) ◽  
pp. 1206-1210 ◽  
Author(s):  
W.-B Li ◽  
C. -H. Zhou ◽  
W. D. Pria ◽  
D. C. Teixeira ◽  
V. S. Miranda ◽  
...  
Keyword(s):  
United States ◽  
Sequence Data ◽  
Xylella Fastidiosa ◽  
Experimental System ◽  
The United States ◽  
Full Genome Sequence ◽  
Pierce's Disease ◽  
Plant Colonization ◽  
Pierce’S Disease ◽  
Citrus Groves

Xylella fastidiosa causes citrus variegated chlorosis (CVC) disease in Brazil and Pierce's disease of grapevines in the United States. Both of these diseases cause significant production problems in the respective industries. The recent establishment of the glassy-winged sharpshooter in California has radically increased the threat posed by Pierce's disease to California viticulture. Populations of this insect reach very high levels in citrus groves in California and move from the orchards into the vineyards, where they acquire inoculum and spread Pierce's disease in the vineyards. Here we show that strains of X. fastidiosa isolated from diseased citrus and coffee in Brazil can incite symptoms of Pierce's disease after mechanical inoculation into seven commercial Vitis vinifera varieties grown in Brazil and California. Thus, any future introduction of the CVC strains of X. fastidiosa into the United States would pose a threat to both the sweet orange and grapevine industries. Previous work has clearly shown that the strains of X. fastidiosa isolated from Pierce's disease- and CVC-affected plants are the most distantly related of all strains in the diverse taxon X. fastidiosa. The ability of citrus strains of X. fastidiosa to incite disease in grapevine is therefore surprising and creates an experimental system with which to dissect mechanisms used by X. fastidiosa in plant colonization and disease development using the full genome sequence data that has recently become available for both the citrus and grapevine strains of this pathogen.

scholarly journals Multilocus Sequence Typing of Xylella fastidiosa Causing Pierce's Disease and Oleander Leaf Scorch in the United States

2010 ◽  
Vol 100 (6) ◽  
pp. 601-611 ◽  
Author(s):  
Xiaoli Yuan ◽  
Lisa Morano ◽  
Robin Bromley ◽  
Senanu Spring-Pearson ◽  
Richard Stouthamer ◽  
...  
Keyword(s):  
United States ◽  
Multilocus Sequence Typing ◽  
Xylella Fastidiosa ◽  
Housekeeping Genes ◽  
The United States ◽  
Pierce's Disease ◽  
Whole Genome Analysis ◽  
Leaf Scorch ◽  
Pierce’S Disease ◽  
Typing Methods

Using a modified multilocus sequence typing (MLST) scheme for the bacterial plant pathogen Xylella fastidiosa based on the same seven housekeeping genes employed in a previously published MLST, we studied the genetic diversity of two subspecies, X. fastidiosa subsp. fastidiosa and X. fastidiosa subsp. sandyi, which cause Pierce's disease and oleander leaf scorch, respectively. Typing of 85 U.S. isolates (plus one from northern Mexico) of X. fastidiosa subsp. fastidiosa from 15 different plant hosts and 21 isolates of X. fastidiosa subsp. sandyi from 4 different hosts in California and Texas supported their subspecific status. Analysis using the MLST genes plus one cell-surface gene showed no significant genetic differentiation based on geography or host plant within either subspecies. Two cases of homologous recombination (with X. fastidiosa subsp. multiplex, the third U.S. subspecies) were detected in X. fastidiosa subsp. fastidiosa. Excluding recombination, MLST site polymorphism in X. fastidiosa subsp. fastidiosa (0.048%) and X. fastidiosa subsp. sandyi (0.000%) was substantially lower than in X. fastidiosa subsp. multiplex (0.240%), consistent with the hypothesis that X. fastidiosa subspp. fastidiosa and sandyi were introduced into the United States (probably just prior to 1880 and 1980, respectively). Using whole-genome analysis, we showed that MLST is more effective at genetic discrimination at the specific and subspecific level than other typing methods applied to X. fastidiosa. Moreover, MLST is the only technique effective in detecting recombination.

scholarly journals Infection of Blueberry Cultivar ‘Emerald’ with a California Pierce’s Disease Strain of Xylella fastidiosa and Acquisition by Glassy-Winged Sharpshooter

Plant Disease ◽  
2020 ◽  
Vol 104 (1) ◽  
pp. 154-160
Author(s):  
Lindsey P. Burbank ◽  
Mark S. Sisterson ◽  
Michael L. O’Leary
Keyword(s):  
United States ◽  
Xylella Fastidiosa ◽  
Southeastern United States ◽  
San Joaquin Valley ◽  
Pierce's Disease ◽  
Highbush Blueberry ◽  
Homalodisca Vitripennis ◽  
Pierce’S Disease ◽  
Fastidiosa Strain ◽  
Southern Highbush

Bacterial leaf scorch disease caused by Xylella fastidiosa occurs in southern highbush blueberry varieties in the southeastern United States. Susceptibility to X. fastidiosa varies by blueberry cultivar, and these interactions are often strain-specific. Xylella fastidiosa subsp. fastidiosa is the causal agent of Pierce’s disease in grapevines, and it has been problematic in the San Joaquin Valley of California since the introduction of the glassy-winged sharpshooter (Homalodisca vitripennis). The glassy-winged sharpshooter is known to feed on blueberry, a crop that is expanding in the San Joaquin Valley. Currently, little is known about the potential for the spread of X. fastidiosa between grape and blueberry in this region. The ability of a Pierce’s disease strain of X. fastidiosa from the San Joaquin Valley to cause disease in southern highbush blueberry and the potential for the glassy-winged sharpshooter to transmit X. fastidiosa between blueberry and grapevine were investigated. Experimental inoculations showed that the X. fastidiosa subsp. fastidiosa strain Bakersfield-1 can cause disease in blueberry cv. Emerald, and that the glassy-winged sharpshooter can acquire X. fastidiosa from artificially inoculated blueberry plants under laboratory conditions. Understanding the possibility for X. fastidiosa strains from the San Joaquin Valley to infect multiple crops grown in proximity is important for area-wide pest and disease management.

scholarly journals Comparative Analyses of the Complete Genome Sequences of Pierce's Disease and Citrus Variegated Chlorosis Strains of Xylella fastidiosa

2003 ◽  
Vol 185 (3) ◽  
pp. 1018-1026 ◽  
Author(s):  
M. A. Van Sluys ◽  
M. C. de Oliveira ◽  
C. B. Monteiro-Vitorello ◽  
C. Y. Miyaki ◽  
L. R. Furlan ◽  
...  
Keyword(s):  
Xylella Fastidiosa ◽  
Chromosomal Rearrangements ◽  
Amino Acid Identity ◽  
Genomic Islands ◽  
Pierce's Disease ◽  
Plant Colonization ◽  
Broad Host Range ◽  
Citrus Variegated Chlorosis ◽  
Comparative Analyses ◽  
Pierce’S Disease

ABSTRACT Xylella fastidiosa is a xylem-dwelling, insect-transmitted, gamma-proteobacterium that causes diseases in many plants, including grapevine, citrus, periwinkle, almond, oleander, and coffee. X. fastidiosa has an unusually broad host range, has an extensive geographical distribution throughout the American continent, and induces diverse disease phenotypes. Previous molecular analyses indicated three distinct groups of X. fastidiosa isolates that were expected to be genetically divergent. Here we report the genome sequence of X. fastidiosa (Temecula strain), isolated from a naturally infected grapevine with Pierce's disease (PD) in a wine-grape-growing region of California. Comparative analyses with a previously sequenced X. fastidiosa strain responsible for citrus variegated chlorosis (CVC) revealed that 98% of the PD X. fastidiosa Temecula genes are shared with the CVC X. fastidiosa strain 9a5c genes. Furthermore, the average amino acid identity of the open reading frames in the strains is 95.7%. Genomic differences are limited to phage-associated chromosomal rearrangements and deletions that also account for the strain-specific genes present in each genome. Genomic islands, one in each genome, were identified, and their presence in other X. fastidiosa strains was analyzed. We conclude that these two organisms have identical metabolic functions and are likely to use a common set of genes in plant colonization and pathogenesis, permitting convergence of functional genomic strategies.

scholarly journals Challenges in evaluating the use of viral sequence data to identify HIV transmission networks for public health

2020 ◽  
Vol 12 (s1) ◽  
Author(s):  
Rami Kantor ◽  
John P. Fulton ◽  
Jon Steingrimsson ◽  
Vladimir Novitsky ◽  
Mark Howison ◽  
...  
Keyword(s):  
Public Health ◽  
United States ◽  
Hiv Transmission ◽  
Sequence Data ◽  
The United States ◽  
Viral Sequence ◽  
Transmission Networks ◽  
New Methods ◽  
Hiv Epidemic ◽  
The World

AbstractGreat efforts are devoted to end the HIV epidemic as it continues to have profound public health consequences in the United States and throughout the world, and new interventions and strategies are continuously needed. The use of HIV sequence data to infer transmission networks holds much promise to direct public heath interventions where they are most needed. As these new methods are being implemented, evaluating their benefits is essential. In this paper, we recognize challenges associated with such evaluation, and make the case that overcoming these challenges is key to the use of HIV sequence data in routine public health actions to disrupt HIV transmission networks.

Planting native trees to restore riparian forests increases biotic resistance to non-native plant invasions

2021 ◽  
pp. 1-24
Author(s):  
Chad F. Hammer ◽  
John S. Gunn
Keyword(s):  
United States ◽  
Ecosystem Service ◽  
Invasive Plant ◽  
Biotic Resistance ◽  
The United States ◽  
Tree Regeneration ◽  
Tree Planting ◽  
Plant Colonization ◽  
Native Plant ◽  
Native Trees

Abstract Non-native invasive plant species are a major cause of ecosystem degradation and impairment of ecosystem service benefits in the United States. Forested riparian areas provide many ecosystem service benefits and are vital to maintaining water quality of streams and rivers. These systems are also vulnerable to natural disturbances and invasion by non-native plants. We assessed whether planting native trees on disturbed riparian sites may increase biotic resistance to invasive plant establishment in central Vermont in the northeastern United States. The density (stems/m2) of invasive stems was higher in non-planted sites (x̄=4.1 stems/m2) compared to planted sites (x̄=1.3 stems/m2). More than 90% of the invasive plants were Japanese knotweed (Fallopia japonica). There were no significant differences in total stem density of native vegetation between planted and non-planted sites. Other measured response variables such as native tree regeneration, species diversity, soil properties and soil function showed no significant differences or trends in the paired riparian study sites. The results of this case study indicate that tree planting in disturbed riparian forest areas may assist conservation efforts by minimizing the risk of invasive plant colonization.

scholarly journals 130. increase in Multidrug Resistance (2011–2018) and the Emergence of Extensive Drug Resistance (2020) in shigella Sonnei in the United States

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S195-S195
Author(s):  
Naeemah Z Logan ◽  
Beth E Karp ◽  
Kaitlin A Tagg ◽  
Claire Burns-Lynch ◽  
Jessica Chen ◽  
...  
Keyword(s):  
United States ◽  
Multidrug Resistance ◽  
Disease Surveillance ◽  
Sequence Data ◽  
The United States ◽  
Multidrug Resistant ◽  
Shigella Sonnei ◽  
Short Read ◽  
Men 2 ◽  
The Us

Abstract Background Multidrug-resistant (MDR) Shigella sonnei infections are a serious public health threat, and outbreaks are common among men who have sex with men (MSM). In February 2020, Australia’s Department of Health notified CDC of extensively drug-resistant (XDR) S. sonnei in 2 Australian residents linked to a cruise that departed from Florida. We describe an international outbreak of XDR S. sonnei and report on trends in MDR among S. sonnei in the United States. Methods Health departments (HDs) submit every 20th Shigella isolate to CDC’s National Antimicrobial Resistance Monitoring System (NARMS) laboratory for susceptibility testing. We defined MDR as decreased susceptibility to azithromycin (MIC ≥32 µg/mL) with resistance to ampicillin, ciprofloxacin, and cotrimoxazole, and XDR as MDR with additional resistance to ceftriaxone. We used PulseNet, the national subtyping network for enteric disease surveillance, to identify US isolates related to the Australian XDR isolates by short-read whole genome sequencing. We screened these isolates for resistance determinants (ResFinder v3.0) and plasmid replicons (PlasmidFinder) and obtained patient histories from HDs. We used long-read sequencing to generate closed plasmid sequences for 2 XDR isolates. Results NARMS tested 2,781 S. sonnei surveillance isolates during 2011–2018; 80 (2.9%) were MDR, including 1 (0.04%) that was XDR. MDR isolates were from men (87%), women (9%), and children (4%). MDR increased from 0% in 2011 to 15.3% in 2018 (Figure). In 2020, we identified XDR isolates from 3 US residents on the same cruise as the Australians. The US residents were 41–42 year-old men; 2 with available information were MSM. The US and Australian isolates were highly related (0–1 alleles). Short-read sequence data from all 3 US isolates mapped to the blaCTX-M-27 harboring IncFII plasmids from the 2 Australian isolates with >99% nucleotide identity. blaCTX-M-27 genes confer ceftriaxone resistance. Increase in Percentage of Shigella sonnei Isolates with Multidrug Resistance* in the United States, 2011–2018† Conclusion MDR S. sonnei is increasing and is most often identified among men. XDR S. sonnei infections are emerging and are resistant to all recommended antibiotics, making them difficult to treat without IV antibiotics. This outbreak illustrates the alarming capacity for XDR S. sonnei to disseminate globally among at-risk populations, such as MSM. Disclosures All Authors: No reported disclosures

scholarly journals Draft Genome Sequence of Xylella fastidiosa subsp. fastidiosa Strain Stag’s Leap

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
J. Chen ◽  
F. Wu ◽  
Z. Zheng ◽  
X. Deng ◽  
L. P. Burbank ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Genome Sequence ◽  
Gene Function ◽  
Xylella Fastidiosa ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Pierce's Disease ◽  
Pierce’S Disease ◽  
Knockout Mutants ◽  
Fastidiosa Strain

Xylella fastidiosa subsp. fastidiosa causes Pierce’s disease of grapevine. Presented here is the draft genome sequence of the Stag’s Leap strain, previously used in pathogenicity/virulence assays to evaluate grapevine germplasm bearing Pierce’s disease resistance and a phenotypic assessment of knockout mutants to determine gene function.

scholarly journals Transmission of the Citrus Variegated Chlorosis Bacterium Xylella fastidiosa with the Sharpshooter Oncometopia nigricans

Plant Disease ◽  
2002 ◽  
Vol 86 (11) ◽  
pp. 1237-1239 ◽  
Author(s):  
R. H. Brlansky ◽  
V. D. Damsteegt ◽  
J. S. Hartung
Keyword(s):  
United States ◽  
Polymerase Chain Reaction ◽  
Xylella Fastidiosa ◽  
The United States ◽  
Reliable Indicator ◽  
Symptom Development ◽  
Chain Reaction ◽  
Citrus Variegated Chlorosis ◽  
Specific Polymerase Chain Reaction ◽  
Polymerase Chain

Citrus variegated chlorosis (CVC) is an economically important, destructive disease in Brazil and is caused by the bacterium Xylella fastidiosa Wells. The bacterium has been found to be transmitted in Brazil by sharpshooter leafhoppers (Cicadellidae). Sharpshooters are present in most citrus growing areas of the United States. The sharpshooter leafhopper, Oncometopia nigricans Walker, frequently is found feeding on citrus in Florida. This sharpshooter transmits the X. fastidiosa strains that cause Pierce's disease of grape and ragweed stunt. Research was initiated to determine if O. nigricans was capable of vectoring the X. fastidiosa that causes CVC. In 59 different transmission tests, using 1 to 57 insects per test, transmission of the bacterium was observed 12 times (20.3%). Symptom development in the greenhouse was not a reliable indicator of transmission. Transmission was verified by specific polymerase chain reaction-based assays. Individual insects were able to transmit the bacterium. This information on sharpshooter transmission of CVC is needed to assess the threat posed by the CVC disease to the citrus industries in the United States.

scholarly journals Complete Genome Sequence of a Human Norovirus Strain from the United States Classified as Genotype GII.P6_GII.6

2018 ◽  
Vol 6 (22) ◽  
Author(s):  
Haifeng Chen ◽  
Shiliang Wang ◽  
Weimin Wang
Keyword(s):  
United States ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Gc Content ◽  
The United States ◽  
Virus Genome ◽  
Full Genome Sequence ◽  
Full Genome ◽  
Virus Genotype

ABSTRACT We report here the complete genome sequence of a GII.6 norovirus strain detected in a clinical fecal specimen from the United States. The virus genome has a length of 7,547 bp and a GC content of 50.1%. Complete norovirus genotyping of the full-genome sequence identified the virus genotype as GII.P6_GII.6.

scholarly journals Grape Cultivar and Sap Culture Conditions Affect the Development of Xylella fastidiosa Phenotypes Associated with Pierce's Disease

PLoS ONE ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. e0160978 ◽  
Author(s):  
Lingyun Hao ◽  
Paulo A. Zaini ◽  
Harvey C. Hoch ◽  
Thomas J. Burr ◽  
Patricia Mowery
Keyword(s):  
Xylella Fastidiosa ◽  
Culture Conditions ◽  
Pierce's Disease ◽  
Grape Cultivar ◽  
Pierce’S Disease
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