scholarly journals Biological and Phylogenetic Analyses Suggest that Two Cryphonectria spp. Cause Cankers of Eucalyptus in Africa

Plant Disease ◽  
2003 ◽  
Vol 87 (11) ◽  
pp. 1329-1332 ◽  
Author(s):  
J. Roux ◽  
H. Myburg ◽  
B. D. Wingfield ◽  
M. J. Wingfield
Keyword(s):  
South Africa ◽  
South America ◽  
South African ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Eucalyptus Grandis ◽  
Its Region ◽  
South African Isolate ◽  
Dna Sequence Data ◽  
The Republic

Cryphonectria cubensis is an economically important pathogen of commercial Eucalyptus spp. Differences have been reported for disease symptoms associated with Cryphonectria canker in South Africa and other parts of the world, and recent DNA-based comparisons have confirmed that the fungus in South Africa is different from that in South America and Australasia. During a disease survey in the Republic of Congo, Cryphonectria canker was identified as an important disease on Eucalyptus grandis and E. urophylla. In this study, we compared Congolese and South African isolates of C. cubensis using DNA sequence data and pathogenicity under greenhouse conditions. The β-tubulin and internal transcribed spacer (ITS) region sequences show that C. cubensis in Congo is different from the fungus in South Africa and that Congolese isolates group most closely with South American isolates. Furthermore, pathogenicity tests showed that a South African isolate was more aggressive than two Congolese isolates. We conclude that two distinct Cryphonectria spp. occur in Africa and hypothesize that the fungus in the Congo probably was introduced into Africa from South America. Both fungi are important pathogens causing disease and death of economically important plantation trees. However, they apparently have different origins and must be treated separately in terms of disease management and quarantine considerations.

Contrasting the population genetic structure of two velvet worm taxa (Onychophora : Peripatopsidae : Peripatopsis) in forest fragments along the south-eastern Cape, South Africa

2017 ◽  
Vol 31 (6) ◽  
pp. 781 ◽  
Author(s):  
Savel R. Daniels ◽  
Megan Dreyer ◽  
Prashant P. Sharma
Keyword(s):  
South Africa ◽  
Population Genetic ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Forest Fragments ◽  
Eastern Cape ◽  
Dna Sequence Data ◽  
Mtdna Coi ◽  
Haplotype Networks ◽  
Velvet Worm

During the present study, we examined the phylogeography and systematics of two species of velvet worm (Peripatopsis Pocock, 1894) in the forested region of the southern Cape of South Africa. A total of 89 P. moseleyi (Wood-Mason, 1879) and 65 P. sedgwicki (Purcell, 1899) specimens were collected and sequenced for the cytochrome c oxidase subunit I mtDNA (COI). In addition, a single P. sedgwicki specimen per sample locality was sequenced for the 18S rRNA locus. Furthermore, morphological variation among P. sedgwicki sample localities were explored using traditional alpha taxonomic characters. DNA sequence data were subjected to phylogenetic analyses using Bayesian inference and population genetic analyses using haplotype networks and analyses of molecular variance (AMOVAs). Phylogenetic results revealed the presence of four and three clades within P. moseleyi and P. sedgwicki respectively. Haplotype networks were characterised by the absence of shared haplotypes between clades, suggesting genetic isolation, a result corroborated by the AMOVA and highly significant FST values. Specimens from Fort Fordyce Nature Reserve were both genetically and morphologically distinct from the two remaining P. sedgwicki clades. The latter result suggests the presence of a novel lineage nested within P. sedgwicki and suggests that species boundaries within this taxon require re-examination.

scholarly journals New host associations and a novel species for the gall-inducing acacia rust genus Ravenelia in South Africa

MycoKeys ◽  
2018 ◽  
Vol 43 ◽  
pp. 1-21 ◽  
Author(s):  
Malte Ebinghaus ◽  
Wolfgang Maier ◽  
Michael J. Wingfield ◽  
Dominik Begerow
Keyword(s):  
South Africa ◽  
Sequence Data ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Principal Component ◽  
Morphological Characters ◽  
Rust Fungi ◽  
New Host ◽  
Dna Sequence Data ◽  
Molecular Phylogenetic

Trees in the genusVachellia(previouslyAcacia) are commonly infected by the gall-inducing rustsRaveneliamacowanianaandR.evansii. Rust galls bearing aecial infections and relating uredinial and telial infections on the leaves of nineVachelliaspecies not previously recorded to be infected byRaveneliaspp. have recently been collected in South Africa. The rust fungi causing these infections were characterised using molecular phylogenetic analyses of DNA sequence data of the LSU and ITS rDNA regions as well as morphological examinations. The host range ofR.macowanianaandR.evansiiwas thus re-assessed and extended from four to nine species and from one to three species, respectively. Application of Principal Component Analyses (PCA) of telial morphological characters provided evidence of an effect of the host species on the teliospore morphology inR.evansii, but only minor effects inR.macowaniana. A novel gall-inducingRaveneliasp. closely related toR.macowaniana, was found onVachelliaxanthophloeaand it is described here asR.xanthophloeae.

Phylogenetic placement and a new circumscription of Poteranthera (Microlicieae; Melastomataceae)

Phytotaxa ◽  
2016 ◽  
Vol 263 (3) ◽  
pp. 219 ◽  
Author(s):  
MARIA JOSÉ REIS DA ROCHA ◽  
PAULO JOSÉ FERNANDES GUIMARÃES ◽  
FABIÁN A. MICHELANGELI ◽  
ROSANA ROMERO
Keyword(s):  
South America ◽  
Dna Sequence ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
New Combination ◽  
Phylogenetic Position ◽  
Glandular Hairs ◽  
Dna Sequence Data ◽  
Phylogenetic Placement ◽  
Generic Description

Poteranthera (Melastomataceae) is a genus of small, annual herbs with narrow leaves, that inhabit humid savannas in South America and are visible only when in bloom. Due to their size and paucity of collections, the phylogenetic position of Poteranthera has been uncertain to this point. Poteranthera has been at times associated, confused, or placed in Acisanthera and/or Siphanthera. Phylogenetic analyses based on DNA sequence data place Poteranthera unequivocally within the Microlicieae and not closely related to Acisanthera or Siphantera. These results are consistent with seed and anther morphology. Putative morphological synapomorphies for this genus are the glandular hairs restricted to the leaf margins and the apically constricted hypanthium. Based on morphology, Acisanthera leptalea seems better placed in Poteranthera and the new combination Poteranthera leptalea is made. An expanded generic description is also provided.

scholarly journals New host associations and a novel species for the gall-inducing acacia rust genus Ravenelia in South Africa

MycoKeys ◽  
2018 ◽  
Vol 42 ◽  
pp. 125-145
Author(s):  
Malte Ebinghaus ◽  
Wolfgang Maier ◽  
Michael J. Wingfield ◽  
Dominik Begerow
Keyword(s):  
South Africa ◽  
Sequence Data ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Principal Component ◽  
Morphological Characters ◽  
Rust Fungi ◽  
New Host ◽  
Dna Sequence Data ◽  
Molecular Phylogenetic

Trees in the genusVachellia(previouslyAcacia) are commonly infected by the gall-inducing rustsRaveneliamacowanianaandR.evansii. Rust galls bearing aecial infections and relating uredinial and telial infections on the leaves of nineVachelliaspecies not previously recorded to be infected byRaveneliaspp. have recently been collected in South Africa. The rust fungi causing these infections were characterised using molecular phylogenetic analyses of DNA sequence data of the LSU and ITS rDNA regions as well as morphological examinations. The host range ofR.macowanianaandR.evansiiwas thus re-assessed and extended from four to nine species and from one to three species, respectively. Application of Principal Component Analyses (PCA) of telial morphological characters provided evidence of an effect of the host species on the teliospore morphology inR.evansii, but only minor effects inR.macowaniana. A novel gall-inducingRaveneliasp. closely related toR.macowaniana, was found onVachelliaxanthophloeaand it is described here asR.xanthophloeae.

scholarly journals Capalictus, a new subgenus of Lasioglossum Curtis 1833 from South Africa, with description of three new species (Hymenoptera, Apoidea, Halictidae)

2012 ◽  
Author(s):  
Alain Pauly ◽  
Jason Gibbs ◽  
Michael Kuhlmann
Keyword(s):  
South Africa ◽  
New Species ◽  
South African ◽  
Dna Sequence ◽  
Type Species ◽  
Sequence Data ◽  
Dna Sequence Data ◽  
New Subgenus ◽  
Basal Clade ◽  
Three New Species

Capalictus, a new subgenus of Lasioglossum Curtis, 1833 (Hymenoptera, Apoidea, Halic-tidae), endemic to the South African Cape Province, is described. The type species is Halictus mosselinus Cockerell, 1945. Evylaeus (Sellalictus) fynbosensis (Pauly et al., 2008) is a new junior synonym of L. (C.) mosselinum. Three new species are described: Lasioglossum (Capalictus) hantamense sp. nov., L. (C.) tigrinum sp. nov. and L. (C.) timmermanni sp. nov. DNA sequence data from three nuclear genes support morphologically-determined species limits. Capalictus is a basal clade of the Hemihalictus series of Lasioglossum.

scholarly journals Phylogenetic Analysis of South African Bovine Leukaemia Virus (BLV) Isolates

Viruses ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 898
Author(s):  
Akiko Suzuki ◽  
Rosamund Chapman ◽  
Nicola Douglass ◽  
Olivia Carulei ◽  
Johan van Rensburg ◽  
...  
Keyword(s):  
South Africa ◽  
Amino Acid ◽  
South African ◽  
Phylogenetic Analyses ◽  
Full Length ◽  
Economic Losses ◽  
Bovine Leukaemia Virus ◽  
Leukaemia Virus ◽  
South African Isolate ◽  
Geographical Regions

Bovine leukaemia virus (BLV) causes chronic lymphoproliferative disorder and fatal lymphosarcoma in cattle, leading to significant economic losses in the beef and dairy industries. BLV is endemic globally and eleven genotypes have been identified. To date, only Zambian isolates have been genotyped from Africa. Although high BLV prevalence has been reported in South Africa, there has been no molecular characterisation of South African BLV isolates. To characterise BLV isolates in South Africa for the first time, we investigated the phylogenetic relationships and compared the genetic variability of eight South African BLV isolates with BLV isolates representing the eleven known genotypes from different geographical regions worldwide. Phylogenetic analyses based on full-length and partial env sequences as well as full-length gag sequences revealed that at least two genotypes, genotypes 1 (G1) and 4 (G4), are present in cattle in South Africa, which is consistent with studies from Zambia. However, our analysis revealed that the G1 South African isolate is more similar to other G1 isolates than the G1 Zambian isolates whereas, the G4 South African isolates are more divergent from other G4 isolates but closely related to the G4 Zambian isolate. Lastly, amino acid sequence alignment identified genotype-specific as well as novel amino acid substitutions in the South African isolates. The detection of two genotypes (G1 and G4) in southern Africa highlights the urgent need for disease management and the development of an efficacious vaccine against local strains.

scholarly journals Taxonomic Re-Examination of Nine Rosellinia Types (Ascomycota, Xylariales) Stored in the Saccardo Mycological Collection

2021 ◽  
Vol 9 (3) ◽  
pp. 666
Author(s):  
Niccolò Forin ◽  
Alfredo Vizzini ◽  
Federico Fainelli ◽  
Enrico Ercole ◽  
Barbara Baldan
Keyword(s):  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Illumina Miseq ◽  
Botanical Garden ◽  
Molecular Study ◽  
Its2 Sequence ◽  
Type Specimens ◽  
Its1 Sequence ◽  
Dna Sequence Data ◽  
Nomen Novum

In a recent monograph on the genus Rosellinia, type specimens worldwide were revised and re-classified using a morphological approach. Among them, some came from Pier Andrea Saccardo’s fungarium stored in the Herbarium of the Padova Botanical Garden. In this work, we taxonomically re-examine via a morphological and molecular approach nine different Roselliniasensu Saccardo types. ITS1 and/or ITS2 sequences were successfully obtained applying Illumina MiSeq technology and phylogenetic analyses were carried out in order to elucidate their current taxonomic position. Only the ITS1 sequence was recovered for Rosellinia areolata, while for R. geophila, only the ITS2 sequence was recovered. We proposed here new combinations for Rosellinia chordicola, R. geophila and R. horridula, while for R. ambigua, R. areolata, R. australis, R. romana and R. somala, we did not suggest taxonomic changes compared to the current ones. The name Rosellinia subsimilis Sacc. is invalid, as it is a later homonym of R. subsimilis P. Karst. & Starbäck. Therefore, we introduced Coniochaeta dakotensis as a nomen novum for R. subsimilis Sacc. This is the first time that these types have been subjected to a molecular study. Our results demonstrate that old types are an important source of DNA sequence data for taxonomic re-examinations.

Safeguarding the Republic? The South African Police Service, Legitimacy and the Tribulations of Policing a Violent Democracy

2021 ◽  
Vol 56 (1) ◽  
pp. 92-108
Author(s):  
Guy Lamb
Keyword(s):  
South Africa ◽  
South African ◽  
Public Trust ◽  
The South ◽  
Police Service ◽  
South African Police Service ◽  
The Republic ◽  
South African Police ◽  
High Crime ◽  
Excessive Use Of Force

Since 1994 the South African Police Service (SAPS) has undertaken various efforts to build legitimacy in South Africa. Extensive community policing resources have been made available, and a hybrid community-oriented programme (sector policing) has been pursued. Nevertheless, public opinion data has shown that there are low levels of public trust in the police. Using Goldsmith’s framework of trust-diminishing police behaviours, this article suggests that indifference, a lack of professionalism, incompetence and corruption on the part of the police, particularly in high-crime areas, have eroded public trust in the SAPS. Furthermore, in an effort to maintain order, reduce crime and assert the authority of the state, the police have adopted militaristic strategies and practices, which have contributed to numerous cases of excessive use of force, which has consequently weakened police legitimacy in South Africa

Revised circumscription of Nothofagus and recognition of the segregate genera Fuscospora, Lophozonia, and Trisyngyne (Nothofagaceae)

Phytotaxa ◽  
2013 ◽  
Vol 146 (1) ◽  
pp. 1 ◽  
Author(s):  
PETER B. HEENAN ◽  
ROB D. SMISSEN
Keyword(s):  
Phylogenetic Analysis ◽  
South America ◽  
Papua New Guinea ◽  
New Genus ◽  
New Caledonia ◽  
Sequence Data ◽  
Morphological Characters ◽  
New Combinations ◽  
Southern South America ◽  
Dna Sequence Data

The generic taxonomy of the Nothofagaceae is revised. We present a new phylogenetic analysis of morphological characters and map these characters onto a recently published phylogenetic tree obtained from DNA sequence data. Results of these and previous analyses strongly support the monophyly of four clades of Nothofagaceae that are currently treated as subgenera of Nothofagus. The four clades of Nothofagaceae are robust and well-supported, with deep stem divergences, have evolutionary equivalence with other genera of Fagales, and can be circumscribed with morphological characters. We argue that these morphological and molecular differences are sufficient for the four clades of Nothofagaceae to be recognised at the primary rank of genus, and that this classification will be more informative and efficient than the currently circumscribed Nothofagus with four subgenera.        Nothofagus is recircumscribed to include five species from southern South America, Lophozonia and Trisyngyne are reinstated, and the new genus Fuscospora is described. Fuscospora and Lophozonia, with six and seven species respectively, occur in New Zealand, southern South America and Australia. Trisyngyne comprises 25 species from New Caledonia, Papua New Guinea and Indonesia. New combinations are provided where necessary in each of these genera.

scholarly journals Dignity, religion and freedom of expression in South Africa

2011 ◽  
Vol 67 (1) ◽  
Author(s):  
Jacobus C.W. Van Rooyen
Keyword(s):  
South Africa ◽  
South African ◽  
Freedom Of Expression ◽  
Sense Of Self ◽  
Point Of View ◽  
National Pride ◽  
Form Part ◽  
Self Worth ◽  
Constitutional Right ◽  
The Republic

The issue that this article dealt with is whether, in South African law, speech that infringes upon the religious feelings of an individual is protected by the dignity clause in the Constitution of the Republic of South Africa. The Constitution, as well as the Broadcasting Code, prohibits language that advocates hatred, inter alia, based on religion and that constitutes incitement to cause harm. Dignity, which is a central Constitutional right, relates to the sense of self worth which a person has. A Court has held that religious feelings, national pride and language do not form part of dignity, for purposes of protection in law. The Broadcasting Complaints Commission has, similarly, decided that a point of view seriously derogatory of ‘Calvinistic people’ blaming (some of) them as being hypocritical and even acting criminally is not protected by dignity. It would have to be accompanied by the advocacy of hatred as defined previously. The author, however, pointed out that on occasion different facts might found a finding in law that religion is so closely connected to dignity, that it will indeed be regarded as part thereof.

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