scholarly journals Potential Sources of Resistance for Melon to Nonpersistently Aphid-borne Viruses

Plant Disease ◽  
2003 ◽  
Vol 87 (8) ◽  
pp. 960-964 ◽  
Author(s):  
Juan Antonio Díaz ◽  
Cristina Mallor ◽  
Carmen Soria ◽  
Rocío Camero ◽  
Elisa Garzo ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Aphis Gossypii ◽  
Zucchini Yellow Mosaic Virus ◽  
Virus Transmission ◽  
Systemic Infection ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Song Type ◽  
Sources Of Resistance ◽  
Potential Sources

Two hundred and sixty-eight Cucumis melo and wild relative accessions were evaluated for resistance to Cucumber mosaic virus (CMV), Papaya ringspot virus strain W (PRSV-W), Watermelon mosaic virus (WMV), and Zucchini yellow mosaic virus (ZYMV). Symptom development and systemic infection based on double antibody sandwich enzyme-linked immunosorbent assays were monitored. Sources of resistance were scarce. However, a number of them were found among the C. melo accessions tested. Thus, the accession C-189 behaved similarly to PI 161375 and showed resistance to “common” CMV strains although it was infected by a “song” type CMV. For WMV, the accessions C-768 and C-425, although infected, exhibited very mild symptoms, and recovery from infection occurred in some occasions in the former. A similar trait was also found in C-105, although in this case symptoms rarely appear and part of the plants are not infected. In addition, C-105 was highly resistant to virus transmission by Aphis gossypii, similarly to PI 161375. Accessions C-885 and C-769 exhibited resistance to PRSV-W, WMV, and ZYMV; therefore they are potential sources of multiple resistance. Resistance traits were also found in wild relatives that could be exploited when interspecific barriers with C. melo can be circumvented.

scholarly journals Evaluation of ‘AU-Performance’ Watermelon for Its Response to Virus Inoculation

2009 ◽  
Vol 19 (3) ◽  
pp. 609-612 ◽  
Author(s):  
John F. Murphy ◽  
Fenny Dane
Keyword(s):  
Mosaic Virus ◽  
Fungal Pathogens ◽  
Citrullus Lanatus ◽  
Zucchini Yellow Mosaic Virus ◽  
Systemic Infection ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Susceptible Parent ◽  
Resistant Parent ◽  
Systemic Symptoms

The watermelon (Citrullus lanatus var. lanatus) ‘AU-Performance’ was developed for resistance to multiple fungal pathogens and the plant virus, zucchini yellow mosaic virus (ZYMV). A greenhouse-based evaluation was carried out to determine the response of ‘AU-Performance’ to inoculation with three important cucurbit (Cucurbitaceae) viruses in the genus Potyvirus: papaya ringspot virus (PRSV), watermelon mosaic virus (WMV), and ZYMV. The evaluation included the resistant parent (PI595203), the susceptible parent (‘AU-Producer’), and varieties AU-Allsweet and Charleston Gray. Each of the three viruses systemically infected ‘AU-Performance’ with 100% infection and development of characteristic systemic symptoms. The susceptible parent (‘AU-Producer’), ‘AU-Allsweet’, and ‘Charleston Gray’ responded similarly with 100% infection and systemic symptoms. In contrast, the resistant parent (PI595203) was resistant to WMV and ZYMV; however, PRSV-inoculated plants developed a systemic infection with accompanied symptoms and high levels of PRSV accumulation in noninoculated leaves. PI595203 was shown in previous studies to be resistant to PRSV. We show in this report that under greenhouse conditions and application of virus by mechanical inoculation, ‘AU-Performance’ was not resistant to infection by the three potyviruses.

scholarly journals Tests for Allelic Relationships Among Potyvirus Resistance Genes in Cucumis sativus

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 841A-841
Author(s):  
Eileen Kabelka ◽  
Rebecca Grumet
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Backcross Progeny ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Susceptible Parent ◽  
Sources Of Resistance ◽  
Dominance Relationships ◽  
Segregation Ratios

Cucumbers and other cucurbit crops are subject to severe losses due to an array of potyviruses, including zucchini yellow mosaic virus (ZYMV), watermelon mosaic virus (WMV), and the watermelon strain of papaya ringspot virus (PRSV-W). Sources of resistance to these viruses have been identified within the cucumber germplasm, including resistance to ZYMV, WMV, and PRSV in `TMG-l'; resistance to PRSV in `Surinam'; and to ZYMV in `Dina'. In this study, we sought to determine the allelic relationships between resistance to PRSV in `Surinam' and `TMG' and resistance to ZYMV in `Dina' and `TMG'. Segregation ratios among F1, F2, and backcross progeny of `Surinam' and `TMG' indicated that the alleles are at the same locus. Similarly, progeny analysis indicates that the alleles for ZYMV resistance in `Dina' and `TMG' are at the same locus. In each case, however, the alleles appear to differ from one another with respect to dominance relationships, symptom expression, and/or response to different viral strains. We are further characterizing these differences by screening progeny of crosses to a common susceptible parent.

scholarly journals High Prevalence of Three Potyviruses Infecting Cucurbits in Oklahoma and Phylogenetic Analysis of Cucurbit Aphid-Borne Yellows Virus Isolated from Pumpkins

Pathogens ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 53
Author(s):  
Vivek Khanal ◽  
Harrington Wells ◽  
Akhtar Ali
Keyword(s):  
Phylogenetic Analysis ◽  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
High Prevalence ◽  
The Us ◽  
Close Relationship ◽  
The Status ◽  
Low Incidence

Field information about viruses infecting crops is fundamental for understanding the severity of the effects they cause in plants. To determine the status of cucurbit viruses, surveys were conducted for three consecutive years (2016–2018) in different agricultural districts of Oklahoma. A total of 1331 leaf samples from >90 fields were randomly collected from both symptomatic and asymptomatic cucurbit plants across 11 counties. All samples were tested with the dot-immunobinding assay (DIBA) against the antisera of 10 known viruses. Samples infected with papaya ringspot virus (PRSV-W), watermelon mosaic virus (WMV), zucchini yellow mosaic virus (ZYMV), and cucurbit aphid-borne-yellows virus (CABYV) were also tested by RT-PCR. Of the 10 viruses, PRSV-W was the most widespread, with an overall prevalence of 59.1%, present in all 11 counties, followed by ZYMV (27.6%), in 10 counties, and WMV (20.7%), in seven counties, while the remaining viruses were present sporadically with low incidence. Approximately 42% of the infected samples were positive, with more than one virus indicating a high proportion of mixed infections. CABYV was detected for the first time in Oklahoma, and the phylogenetic analysis of the first complete genome sequence of a CABYV isolate (BL-4) from the US showed a close relationship with Asian isolates.

scholarly journals Cucurbit aphid-borne yellows virus Is Prevalent in Field-Grown Cucurbit Crops of Southeastern Spain

Plant Disease ◽  
2007 ◽  
Vol 91 (3) ◽  
pp. 232-238 ◽  
Author(s):  
M. A. Kassem ◽  
R. N. Sempere ◽  
M. Juárez ◽  
M. A. Aranda ◽  
V. Truniger
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Rflp Analysis ◽  
Yellow Mosaic Virus ◽  
Multiple Infections ◽  
Watermelon Mosaic Virus ◽  
Limited Information ◽  
Growing Seasons ◽  
Beet Pseudo Yellows Virus ◽  
The Impact

Despite the importance of field-grown cucurbits in Spain, only limited information is available about the impact of disease on their production. During the 2003 and 2004 growing seasons, systematic surveys were carried out in open field melon (Cucumis melo) and squash (Cucurbita pepo) crops of Murcia Province (Spain). The fields were chosen with no previous information regarding their sanitation status, and samples were taken from plants showing viruslike symptoms. Samples were analyzed using molecular hybridization to detect Beet pseudo-yellows virus (BPYV), Cucurbit aphid-borne yellows virus (CABYV), Cucumber mosaic virus (CMV), Cucumber vein yellowing virus (CVYV), Cucurbit yellow stunting disorder virus (CYSDV), Melon necrotic spot virus (MNSV), Papaya ringspot virus (PRSV), Watermelon mosaic virus (WMV), and Zucchini yellow mosaic virus (ZYMV). We collected 924 samples from 48 field plots. Out of these, almost 90% were infected by at least one of the viruses considered, usually CABYV, which was present in 83 and 66% of the melon and squash samples, respectively. In the case of melon, CYSDV, BPYV, and WMV followed CABYV in relative importance, with frequencies of around 20 to 30%, while in squash, CVYV and BPYY showed frequencies between 28 and 21%. The number of multiple infections was very high, 66 and 56% of the infected samples of melon and squash, respectively, being afflicted. CABYV was present in all multiple infections. The high incidence of CABYV in single and multiple infections suggests that this virus may well become an important threat for cucurbit crops in the region. Restriction fragment length polymorphism (RFLP) analysis revealed that CABYV isolates can be grouped into two genetic types, both of which seemed to be present during the 2003 epidemic episode, but only one of the types was found in 2004.

scholarly journals First Report of Papaya ringspot virus and Zucchini yellow mosaic virus in Luohanguo (Siraitia grosvenorii) in China

Plant Disease ◽  
2005 ◽  
Vol 89 (5) ◽  
pp. 530-530 ◽  
Author(s):  
Y.-M. Liao ◽  
X.-J. Gan ◽  
B. Chen ◽  
J.-H. Cai
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Viral Disease ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Rt Pcr ◽  
First Report ◽  
Siraitia Grosvenorii

Luohanguo, Siraitia grosvenorii (Swingle) C. Jeffrey, is a perennial cucurbitaceous plant that is an economically important medicinal and sweetener crop in Guangxi province, China. Surveys conducted during the summer to fall seasons of 2003-2004 in northern Guangxi showed symptoms typical of a viral disease, including leaf mottling, mosaic, vein clearing, curling, and shoestring-like distortion in the field. Mechanical inoculation of sap from leaves of symptomatic plants collected from the surveyed areas caused similar symptoms on tissue culture-derived healthy Luohanguo plants. Two sequences of 0.7 and 1.6 kb with 88 and 97% identity to Papaya ringspot virus (PRSV) and Zucchini yellow mosaic virus (ZYMV) were amplified using reverse transcription-polymerase chain reaction (RT-PCR) with purified flexuous viral particles or total RNA extracted from the symptomatic Luohanguo leaves as templates with conserved degenerate potyvirus primers (1). To confirm the results, primers specific for PRSV (PP1/PP2, genome coordinates 4064-4083/5087-5069, GenBank Accession No X97251) and ZYMV (ZP1/ZP2, genome coordinates 5540-5557/7937-7920, GenBank Accession No L31350) were used to perform RT-PCR from the same RNA templates. The expected 1.0- and 2.3-kb fragments were amplified and they were 90 and 95% identical to PRSV and ZYMV in sequence, respectively. Watermelon mosaic virus was not detected. To our knowledge, this is the first report of the occurrence of PRSV and ZYMV in Luohanguo. Reference: (1) A. Gibbs et al. J. Virol. Methods 63:9, 1997.

scholarly journals Field Performance Comparison of Two Transgenic Summer Squash Hybrids to Their Parental Hybrid Line

HortScience ◽  
1995 ◽  
Vol 30 (3) ◽  
pp. 492-493 ◽  
Author(s):  
Juan Pablo Arce-Ochoa ◽  
Frank Dainello ◽  
Leonard M. Pike ◽  
David Drews
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Field Performance ◽  
Performance Comparison ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Parental Line ◽  
Summer Squash ◽  
Transgenic Squash ◽  
Marketable Fruit

`Pavo', a commercially grown, virus-susceptible squash (Cucurbita pepo L.) hybrid, and two experimental virus-resistant transgenic squash hybrids, XPH-1719 and XPH-1739, were tested for field performance. The two transgenic squash hybrids possess the desired fruit and plant characteristics of their parental line, `Pavo', plus resistance to zucchini yellow mosaic virus and watermelon mosaic virus 2 (XPH-1719), and resistance to zucchini yellow mosaic virus, watermelon mosaic virus 2, and cucumber mosaic virus (XPH-1739). Percent emergence and days to flowering were similar among the three hybrids. XPH-1719 and XPH-1739 were equally effective in producing a high percentage of quality marketable fruit and yields with 90% and 13,800 kg·ha–1 and 87% and 16,500 kg·ha–1, respectively. XPH-1719 and XPH-1739 demonstrated their outstanding virus resistance over `Pavo' by producing only 3% and 14% symptomatic plants, respectively, compared to 53% for `Pavo'. They also produced the lowest percentage of infected fruit, 0% and 7%, respectively, with `Pavo' at 26%.

scholarly journals Yield and Virus Resistance of Summer Squash Cultivars and Breeding Lines in North Carolina

1998 ◽  
Vol 8 (1) ◽  
pp. 31-39 ◽  
Author(s):  
Jonathan R. Schultheis ◽  
S. Alan Walters
Keyword(s):  
North Carolina ◽  
Mosaic Virus ◽  
Virus Resistance ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Breeding Lines ◽  
Summer Squash

Yellow and zucchini squash (Cucurbita pepo L.) cultigens (breeding lines and cultivars) were evaluated over a 2-year (1995 and 1996) period in North Carolina. Yellow squash cultigens that performed well (based on total marketable yields) were `Destiny III', `Freedom III', `Multipik', XPHT 1815, and `Liberator III' in Fall 1995 and HMX 4716, `Superpik', PSX 391, `Monet', `Dixie', XPH 1780, and `Picasso' in Spring 1996. Some of the yellow squash cultigens evaluated had superior viral resistance: XPHT 1815, XPHT 1817, `Freedom III', `Destiny III', `Freedom II', TW 941121, `Prelude II', and `Liberator III' in Fall 1995 and XPHT 1815, `Liberator III', `Prelude II', and `Destiny III' in Fall 1996; all these cultigens were transgenic. The yellow squash cultigens that performed well (based on total marketable yields) in the Fall 1995 test had transgenic virus resistance (`Destiny III', `Freedom III', XPHT 1815, and `Liberator III') or had the Py gene present in its genetic background (`Multipik'). Based on total marketable yields, the best zucchini cultigens were XPHT 1800, `Tigress', XPHT 1814, `Dividend' (ZS 19), `Elite', and `Noblesse' in Fall 1995; and `Leonardo', `Tigress', `Hurricane', `Elite', and `Noblesse' in Spring 1996. The zucchini cultigens with virus resistance were TW 940966, XPHT 1814, and XPHT 1800 in Fall 1995 and XPHT 1800, XPHT 1776, XPHT 1777, XPHT 1814, and XPHT 1784 in Fall 1996. Even though TW 940966 had a high level of resistance in the Fall 1995 test, it was not as high yielding as some of the more susceptible lines. Viruses detected in the field were papaya ringspot virus (PRSV) and watermelon mosaic virus (WMV) for Fall 1995; while PRSV, zucchini yellow mosaic virus (ZYMV), and WMV were detected for Fall 1996. Summer squash cultigens transgenic for WMV and ZYMV have potential to improve yield, especially during the fall when viruses are more prevalent. Most transgenic cultigens do not possess resistance to PRSV, except XPHT 1815 and XPHT 1817. Papaya ringspot virus was present in the squash tests during the fall of both years. Thus, PRSV resistance must be transferred to the transgenic cultigens before summer squash can be grown during the fall season without the risk of yield loss due to viruses.

Sign in / Sign up

Export Citation Format

Share Document