Susceptibility of Arabidopsis Ecotypes to Infection by Iris yellow spot virus

Iris yellow spot virus (IYSV; genus Tospovirus, family Bunyaviridae) is a serious constraint for onion production in the United States and several other parts of the world. A few experimental indicator hosts were reported for IYSV but there is no report of infection of Arabidopsis by IYSV. Establishing IYSV infection in onion by mechanical inoculation has so far proven to be inefficient resulting in numerous escapes. Using Arabidopsis thaliana as a host for studies on the biology and tospovirus-host interactions has an advantage of the plant's small genome size, short life cycle, and a genome that has been sequenced. Several ecotypes were evaluated for their susceptibility to IYSV and identified some where IYSV produces localized infection. Together with wild type and various mutants, the IYSV-Arabidopsis system could be useful for studying host-tospovirus interactions. Accepted for publication 12 June 2012. Published 14 July 2012.

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First Report of Iris yellow spot virus on Garlic in India

Plant Disease ◽

10.1094/pdis-94-8-1066c ◽

2010 ◽

Vol 94 (8) ◽

pp. 1066-1066 ◽

Cited By ~ 9

Author(s):

S. J. Gawande ◽

A. Khar ◽

K. E. Lawande

Keyword(s):

Natural Infection ◽

The United States ◽

Iris Yellow Spot Virus ◽

Yellow Spot ◽

N Gene ◽

Spot Virus ◽

First Report ◽

Qiagen Gmbh ◽

The World ◽

Seed Crops

Garlic (Allium sativum) is a spice crop of prime importance in India as well as other parts of the world. Iris yellow spot virus (IYSV; genus Tospovirus, family Bunyaviridae) is an important pathogen of onion bulb and seed crops in many parts of the world (3). The virus is also known to infect garlic and other Allium spp. (2–4). IYSV infection of garlic was reported from Reunion Island (4) and the United States (1). In February 2010, straw-colored, spindle-shaped spots with poorly defined ends were observed on the leaves of a garlic crop at the research farm of the Directorate of Onion and Garlic Research in the Pune District of Maharashtra State, India, 105 days after planting. The spots coalesced to form larger patches on the leaves, suggesting possible IYSV infection. Symptoms were visible on older leaves and more prevalent on cv. G-41, G-282, AC50, AC200, AC283, and Godavari than on other cultivars. The incidence of symptomatic plants was estimated at 5% for G-41 and AC-200, 8% for G-282 and AC283, and 10% for AC50. Leaves were sampled from 40 symptomatic plants per cultivar with each sample composited from young, middle, and older (basal) leaves of the plant. Samples were assayed by double-antibody sandwich-ELISA (Loewe Biochemica GmbH, Sauerlach, Germany) and each tested positive for the virus. Total RNA was extracted from the leaves of ELISA-positive plants using the RNAeasy Plant Mini kit (Qiagen GmbH, Hilden, Germany) and tested by reverse transcription-PCR assay using primers IYSV-F (5′-TCAGAAATCGAGAAACTT-3′) and IYSV-R (5′-TAATTATATCTATCTTTCTTGG-3′) (2) designed to amplify 797 bp of the nucleocapsid (N) gene of IYSV. Amplicons of expected size were obtained and cloned into a pDrive vector (Qiagen GmbH). The recombinant clone was sequenced (GenBank Accession No. HM173691). Sequence comparisons showed 98 to 100% nt identity with other IYSV N gene sequences in GenBank (Nos. EU310294 and EU310286). A phylogenetic analysis of the deduced amino acid sequences of the N gene showed that the garlic isolate of IYSV grouped most closely with onion IYSV isolates from India (GenBank Nos. EU310294, EU310286, EU310300, and EU310296). To our knowledge, this is the first report of natural infection of garlic by IYSV in India. Additional surveys and evaluations are needed to obtain a better understanding of the potential impact of IYSV on garlic production in India. References: (1) S. Bag et al. Plant Dis. 93:839, 2009. (2) A. Bulajic et al. Plant Dis. 93:976, 2009. (3) D. Gent et al. Plant Dis. 90:1468, 2006. (4) I. Robène-Soustrade et al. Plant Pathol. 55:288, 2006.

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Natural Infection of Iris yellow spot virus in Twoscale Saltbush (Atriplex micrantha) Growing in Utah

Plant Disease ◽

10.1094/pdis-93-4-0430c ◽

2009 ◽

Vol 93 (4) ◽

pp. 430-430 ◽

Cited By ~ 7

Author(s):

C. K. Evans ◽

S. Bag ◽

E. Frank ◽

J. R. Reeve ◽

C. Ransom ◽

...

Keyword(s):

Natural Infection ◽

Sandwich Elisa ◽

The United States ◽

Iris Yellow Spot Virus ◽

Yellow Spot ◽

N Gene ◽

Nucleotide Sequence Analysis ◽

Jefferson County ◽

Spot Virus ◽

Sequence Identity

Iris yellow spot virus (IYSV; family Bunyaviridae, genus Tospovirus) continues to be an economically important pathogen affecting onion bulb and seed production in several parts of the world and the United States (1). Several weeds were reported naturally infected with IYSV (1,2,4). Leaves of Atriplex micrantha Ledeb. (synonym A. heterosperma Bunge) were collected from naturally occurring plants in a weed trial conducted in commercial onions grown in Box Elder County, UT on 24 September 2008. Leaves displayed a range of symptoms including spotting, chlorosis, and necrosis. Symptomatic leaves were preferentially selected for subsequent diagnostic analyses. Samples were positive for IYSV when tested by double-antibody sandwich-ELISA using a commercially available kit (Agdia Inc., Elkhart, IN). For further confirmation, total nucleic acid extracts from the symptomatic parts of the leaves were prepared and tested for the presence of IYSV by reverse transcription-PCR with primers specific to the nucleocapsid (N) gene coded by the small (S)-RNA of IYSV. The forward and reverse primer pair, 5′-TCAGAAATCGAGAAACTT-3′ and 5′-CACCAATGTCTTCAACAATCTT-3′, respectively, amplifies a 751-nt fragment of the N gene (3). An amplicon of expected size was obtained, cloned, and sequenced. The nucleotide sequence analysis and comparison with known IYSV S-RNA sequences showed that the sequence of the amplicon from A. micrantha (GenBank Accession No. FJ493541) shared more than 84% nt sequence identity with the corresponding region of IYSV isolates available in GenBank, confirming the IYSV infection of the new host weed. The highest sequence identity (98%) was with an IYSV isolate from Jefferson County, OR (GenBank Accession No. DQ233479). To our knowledge, this is the first report of IYSV infection of A. micrantha under natural conditions. The role of A. micrantha and other weeds in IYSV epidemiology needs further investigation. References: (1) D. Gent et al. Plant Dis. 90:1468, 2006. (2) C. Nischwitz et al. Plant Dis. 91:1518, 2007. (3) H. R. Pappu et al. Arch. Virol. 151:1015, 2006. (4) R. Sampangi et al. Plant Dis. 91:1683, 2007.

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Iris yellow spot virus in Onion in Chile

Plant Disease ◽

10.1094/pd-89-1245c ◽

2005 ◽

Vol 89 (11) ◽

pp. 1245-1245 ◽

Cited By ~ 9

Author(s):

M. Rosales ◽

H. R. Pappu ◽

L. López ◽

R. Mora ◽

A. Aljaro

Keyword(s):

The United States ◽

Iris Yellow Spot Virus ◽

Yellow Spot ◽

Enzyme Linked Immunosorbent Assay ◽

Spot Virus ◽

Sequence Comparisons ◽

Central Chile ◽

Online Archive ◽

Polymerase Chain ◽

Np Gene

Iris yellow spot virus (IYSV) (genus Tospovirus, family Bunyaviridae) has become endemic in several parts of the world. Between 2000 and 2004, the virus was reported from several states in the United States and the most recent report was from Georgia (2). In South America, IYSV was first reported from Brazil (3). Symptoms indicative of IYSV infection (1), such as diamond-shaped and irregular chlorotic and necrotic lesions, were observed during 2004 and 2005 in onion fields in Colina and Tiltil (Chacabuco Province) and Rengo (Cachapoal Province) located in central Chile. In the sampled fields, as much as 50% of the crop showed symptoms. In all three locations, the crop was destined for bulb production and the cultivar was a long-day Valenciana-type. Approximately 50 and 5 ha in Chacabuco and Cachapoal provinces, respectively, were surveyed. Symptomatic plants were collected and tested in the laboratory for confirmation of IYSV infection. A commercially available IYSV-specific enzyme-linked immunosorbent assay kit was used (Adgen Limited, Ayr, Scotland, UK) that confirmed the samples were infected with IYSV. Total nucleic acids were extracted, and using primers flanking the nucleoprotein (NP) gene of IYSV, reverse transcription-polymerase chain reaction was used to verify virus identity. Primer sequences were 5′-TAA AAC AAA CAT TCA AAC AA-3′ and 5′-CTC TTA AAC ACA TTT AAC AAG CAC-3′. The resulting 1.2-kb DNA amplicons from five samples were cloned and sequenced (GenBank Accession No. DQ150107). Nucleotide sequence comparisons with known IYSV NP gene sequences showed 95 to 98% identity, confirming the virus as IYSV. The presence of IYSV in Brazil and now in Chile indicates its potential to become established in onion crops in the region. References: (1) L. J. du Toit et al. APSnet Image of the Week. http://apsnet.org/online/archive/2003/IW000030.asp , 2003. (2) S. W. Mullis et al. Plant Dis. 88:1285, 2004. (3) L. Pozzer et al. Plant Dis. 83:345, 1999.

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Onion ipmPIPE: A Coordinated Effort to Improve the Management of Onion Thrips and Iris yellow spot virus for the U.S. Onion Industry

Plant Health Progress ◽

10.1094/php-fe-14-0026 ◽

2014 ◽

Vol 15 (4) ◽

pp. 172-183 ◽

Cited By ~ 1

Author(s):

Howard F. Schwartz ◽

Diane Alston ◽

Jeff Alwang ◽

Michael Bartolo ◽

Tamla Blunt ◽

...

Keyword(s):

United States ◽

Pest Management ◽

The United States ◽

Iris Yellow Spot Virus ◽

Plant Diseases ◽

Yellow Spot ◽

Onion Thrips ◽

Spot Virus ◽

Transmitted Virus ◽

The U.S

Onion (Allium cepa) is an economically important vegetable crop in the United States, generating nearly $900 million annually in farm receipts. Pests such as onion thrips (Thrips tabaci) and a thrips-transmitted virus disease, Iris yellow spot (IYS), caused by Iris yellow spot virus (IYSV), have emerged in recent years as high priority, invasive, or potential threats to sustainable onion production throughout the United States and the world. The long-term goal of a recent United States Department of Agriculture (USDA)-National Institute of Food and Agriculture (NIFA)-Specialty Crop Research Initiative (SCRI) funded project was to optimize sound pest management decision making in onion through the development and deployment of a sustainable online information management platform called the Onion ipmPIPE (Onion integrated pest management Pest Information Platform for Extension and Education). Project participants in seven states generated an average of 500 reports annually on the occurrence of 20 priority insect pests and plant diseases, and enhanced the timeliness and effectiveness of IPM strategies implemented by the growers and industry locally and regionally in response to these reports. Thrips populations are generally greatest at all locations during July and August of each year, which compounds plant responses to environmental stresses such as temperature and limited or lower quality (e.g., saline) irrigation water. The increase in numbers of thrips (majority being T. tabaci) also aggravates problems with the onion thrips-transmitted virus IYSV (mentioned above) and IYS, which generally increases in incidence and severity during this same period. A comprehensive website (http//:www.alliumnet.com/IPMPipe.html) and educational materials (bulletin, diagnostic cards, videos) were developed in response to the insect and disease threats, and have been shared with onion stakeholders throughout the United States and elsewhere. The project is also developing risk assessment models, a smartphone app, onion growing-degree-day review for efficacy of the current model based on 5.5°C from planting, and enhanced pathogen diagnostic tools for future testing and validation by onion stakeholders. Visiting the Onion ipmPIPE website was estimated by respondents to increase onion yields by 280 kg/hectare and reduce pesticide costs by $910/hectare on average. The Onion ipmPIPE platform is scale neutral, and supports a range of organic and conventional growers producing onions on small plots to large-scale commercial fields. Increased participation of stakeholders, including consumers, in a national educational project such as Onion ipmPIPE will better serve current and future interests in sustainability and profitability of critical food crops for the U.S. marketplace. Accepted 25 September 2014. Published 12 November 2014.

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Iris yellow spot virus (Iris yellow spot).

10.1079/cpc.28848.20210100052 ◽

2021 ◽

Author(s):

Keyword(s):

The Netherlands ◽

Cropping Systems ◽

Molecular Data ◽

Iris Yellow Spot Virus ◽

Yellow Spot ◽

Economic Damage ◽

Spot Virus ◽

The World ◽

The Usa ◽

Serological Data

Abstract In 1981, de Avila et al. (1981) described a disease characterized by chlorotic and necrotic, eye-like or diamond-shaped lesions on onion scapes (referred to as 'sapeca') in southern Brazil. In 1989, Hall et al. (1993) observed a very similar disease in onion in the USA and detected a tospovirus, which was later shown by Moyer et al. (1993) to be Iris yellow spot virus on the basis of molecular and serological data. In 1998, a new tospovirus was isolated and characterized in the Netherlands from infected iris and leek and named Iris yellow spot virus (IYSV) (Cortês et al., 1998). This virus was subsequently found naturally infecting onion in several major onion-producing states of the USA and around the world (for reviews, see Gent et al., 2006 and Pappu et al., 2009). Gera et al. (1998b) reported that IYSV was responsible for a 'straw bleaching' disease on onion in Israel. In 1999, a 'sapeca' isolate from Brazil was identified as IYSV on the basis of biological, serological and molecular data (Pozzer et al., 1999). In Israel, Kritzman et al. (2000) reported natural IYSV infection of lisianthus grown in the field. IYSV has now been endemic in south-western Idaho and eastern Oregon in onion, leek and chive seed production fields for over 10 years. Losses caused by IYSV can reach 100% in onion crops, for example, in Brazil (Pappu et al., 2009). However, studies in the Netherlands in 2008 showed that latent infections of IYSV were common in onion crops but did not cause economic damage (NPPO of the Netherlands, 2008). Iris yellow spot represents an immediate and serious threat to sustainable and productive onion cropping systems around the world, and the recent detection of this disease in numerous onion-producing countries demonstrates that the disease is spreading rapidly in a range of environments. IYSV is on the EPPO Alert list (http://www.eppo.org/QUARANTINE/Alert_List/alert_list.htm).

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Serological and Molecular Assays for Rapid and Sensitive Detection of Iris yellow spot virus Infection of Bulb and Seed Onion Crops

Plant Disease ◽

10.1094/pdis-92-4-0588 ◽

2008 ◽

Vol 92 (4) ◽

pp. 588-594 ◽

Cited By ~ 24

Author(s):

H. R. Pappu ◽

I. M. Rosales ◽

K. L. Druffel

Keyword(s):

Real Time ◽

Rapid Detection ◽

The United States ◽

Iris Yellow Spot Virus ◽

Yellow Spot ◽

Sensitive Detection ◽

Detection Methods ◽

Enzyme Linked Immunosorbent Assay ◽

Rt Pcr ◽

Spot Virus

Iris yellow spot virus (IYSV) has spread rapidly in the United States and has become an important economic constraint to the production of both bulb and seed onion crops. Symptoms caused by IYSV may be confused with those caused by other fungal and bacterial pathogens and virus-specific, reliable, sensitive, and rapid detection methods would improve the diagnosis. Antiserum was produced to Escherichia coli-expressed nucleocapsid protein of IYSV and an indirect format of the enzyme-linked immunosorbent assay (ELISA) was developed. IYSV could be detected in onion tissue at dilutions of up to 1:1,000. An IYSV-specific primer pair was designed and used in a real-time reverse-transcription polymerase chain reaction (RT-PCR) assay for the rapid detection of IYSV. Compared with standard RT-PCR, real-time RT-PCR was more rapid and sensitive. A commercially available RNA extraction kit and a total nucleic acid extraction method were compared for the quality of the templates obtained for use in real-time RT-PCR and there was no difference in limits of detection. Availability of ELISA- and PCR-based rapid and sensitive detection methods would facilitate accurate virus diagnosis and aid in better understanding of the epidemiology of the disease and in development of management strategies.

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First Report of Iris yellow spot virus in Commercial Leek (Allium porrum) in the United States

Plant Disease ◽

10.1094/pd-91-0113b ◽

2007 ◽

Vol 91 (1) ◽

pp. 113-113 ◽

Cited By ~ 5

Author(s):

H. F. Schwartz ◽

K. Otto ◽

H. R. Pappu

Keyword(s):

United States ◽

The United States ◽

Iris Yellow Spot Virus ◽

Yellow Spot ◽

N Gene ◽

Allium Porrum ◽

Rt Pcr ◽

Spot Virus ◽

Disease Symptoms ◽

First Report

Iris yellow spot virus (IYSV; family Bunyaviridae, genus Tospovirus) has a wide host range, with onion (Allium cepa L.) being one of the most economically important hosts. IYSV has been widely reported from this species throughout most onion-production regions of the United States and many areas of the world in recent years. A relative of onion, leek (Allium porrum L.), has been reported to be a host of IYSV in countries such as the Netherlands, Reunion Island, and Australia (1,4). A related tospovirus, Tomato spotted wilt virus (TSWV), was recently reported causing necrotic lesions and extended bleaching of leaf tips of leek in Georgia (2). In September of 2006, disease symptoms suspected to be caused by IYSV were observed on central and outer leaves of plants in a 2.6-ha section of commercial leeks being grown from seed (cvs. Tadorna and King Richard). The leek plants were adjacent to a 3.1-ha section of seeded onion (cv. Exacta) that had been harvested 2 weeks earlier. Twenty-five to thirty percent of unharvested onion plants next to the leek section also exhibited IYSV-type disease symptoms generally on the central leaves. Both Allium spp. were seeded 5 months earlier and grown under certified organic, pivot-irrigated conditions in Larimer County in northern Colorado. Disease symptoms on leek and onion leaves appeared as dry, white-to-straw-colored, spindle- or diamond-shaped lesions that ranged in size from 5 to 10 × 25 to 50 mm or larger depending on lesion age. Lesion centers, especially on leek, often had green centers with concentric rings of alternating green and straw-colored tissue. Green tissue near necrotic lesions of a single symptomatic leaf from 10 plants each of leek and onion was sampled and analyzed using a double-antibody sandwich (DAS)-ELISA (Agdia, Inc., Elkhart, IN). Five of ten leek and nine of ten onion samples were positive for IYSV. Using reverse transcription (RT)-PCR and primers specific to the small RNA of IYSV (5′-TAA AAC AAA CAT TCA AAC AA-3′ and 5′-CTC TTA AAC ACA TTT AAC AAG CAC-3′), the complete nucleocapsid (N) gene was amplified from symptomatic leek plants and then sequenced (3). Comparisons with IYSV N gene sequences available in the GenBank confirmed the identity of the virus as IYSV. Leek samples were negative for TSWV when tested by RT-PCR with TSWV-specific primers. In addition, three specimens of the presumed thrips vector recovered from five IYSV-infected leek plants were identified as Thrips tabaci (L. A. Mahaffey and W. S. Cranshaw, personal communication). Earlier in the season, T. tabaci was observed in the nearby planting of onion that also exhibited IYSV in September. To our knowledge, this is the first report of natural infection of commercial leek with IYSV in the United States. The incidence of plants (25 to 30%) with foliar lesions on multiple leaves and stunting of 5% of infected plants in both leek cultivars suggests that IYSV could seriously reduce leek stem development and marketability. References: (1) I. Cortes et al. Phytopathology 88:1276, 1998. (2) C. Nischwitz et al. Plant Dis. 90:525, 2006. (3) H. R. Pappu et al. Arch. Virol. 151:1015, 2006. (4) T. N. Smith et al. Plant Dis. 90:729, 2006.

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First Report of Natural Infection of Garlic with Iris yellow spot virus in the United States

Plant Disease ◽

10.1094/pdis-93-8-0839a ◽

2009 ◽

Vol 93 (8) ◽

pp. 839-839 ◽

Cited By ~ 5

Author(s):

S. Bag ◽

P. Rogers ◽

R. Watson ◽

H. R. Pappu

Keyword(s):

United States ◽

Natural Infection ◽

Sandwich Elisa ◽

The United States ◽

Iris Yellow Spot Virus ◽

Yellow Spot ◽

N Gene ◽

Spot Virus ◽

Sequence Comparisons ◽

First Report

Iris yellow spot virus (IYSV; family Bunyaviridae, genus Tospovirus) is an important constraint to onion bulb and seed production in several onion-growing regions of the United States (1,3). While garlic (Allium sativum) was reported to be infected with IYSV in Réunion Island (4), there have been no confirmed reports of natural infection of garlic in the United States. Garlic plants showing near-diamond-shaped lesions were found in August of 2008 in Marion County, Oregon. The 0.4046-ha (1-acre) field plot consisted of various true-seeded garlic varieties and was adjacent to three onion fields that showed IYSV symptoms. Symptoms were observed on 5% of the garlic plants with most of the symptomatic plants displaying small and diffuse straw-colored spots. Seven of these symptomatic plants were selected for testing. Of these, two showed characteristic diamond-shaped, elongated, straw-colored lesions on garlic scapes. However, the lesions were more diffuse with less-defined edges compared with the characteristic diamond-shaped lesions that are often associated with IYSV infection (1). All symptomatic plants were positive for IYSV by double-antibody sandwich-ELISA with a commercially available kit (Agdia Inc., Elkhart, IN). To verify IYSV infection, total nucleic acid extracts from the symptomatic parts of the leaves were prepared and tested for the presence of IYSV by reverse transcription (RT)-PCR with primers 5′-TAAAACAAACATTCAAACAA-3′ and 5′-CTCTTAAACACATTTAACAAGCAC-3′, which flank the nucleocapsid (N) gene coded by the small RNA of IYSV (2). An approximate 1.1-kb amplicon was obtained from all symptomatic plants and cloned and sequenced. Nucleotide sequence comparisons using BLAST showed that a consensus of three clones derived from the amplicon from garlic (No. FJ514257) was 85 to 99% identical with IYSV sequences available in GenBank (Nos. AF001387, AB180918, and AB286063), confirming the identity of IYSV. To our knowledge, this is the first report of natural infection of IYSV infection of garlic in the United States. Additional surveys and testing are needed to obtain a better understanding of IYSV incidence in garlic to evaluate its impact on garlic production. References: (1) D. Gent et al. Plant Dis. 90:1468, 2006. (2) H. R. Pappu et al. Arch. Virol. 151:1015, 2006. (3) H. R. Pappu et al. Virus Res. 141:219, 2009. (4) I. Robène-Soustrade et al. Plant Pathol. 55:288, 2006.

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First Report of Iris yellow spot virus in Spiny Sowthistle (Sonchus asper) in the United States

Plant Disease ◽

10.1094/pdis-91-11-1518c ◽

2007 ◽

Vol 91 (11) ◽

pp. 1518-1518 ◽

Cited By ~ 12

Author(s):

C. Nischwitz ◽

R. D. Gitaitis ◽

S. W. Mullis ◽

A. S. Csinos ◽

D. B. Langston ◽

...

Keyword(s):

The United States ◽

Thrips Tabaci ◽

Iris Yellow Spot Virus ◽

Yellow Spot ◽

Insect Vector ◽

Spot Virus ◽

Nucleocapsid Gene ◽

First Occurrence ◽

Winter Crop ◽

Reverse Primer

Iris yellow spot virus (IYSV) is a member of the genus Tospovirus in the family Bunyaviridae. Its known host range is very limited, and the currently known hosts include onion, leek, lisianthus, and alstroemeria (2). The virus is vectored by onion thrips (Thrips tabaci). Onion (Allium cepa) is grown as a winter crop in Georgia from September to April and is the only known host commercially grown in the region. However, the virus has been found across the onion-growing region in the state every year since its first occurrence during 2003 (3). Consequently, the virus must oversummer in other host(s) or its insect vector. Accordingly, samples of weeds were collected in the vicinity of onion fields and cull piles in the Vidalia region and tested for the presence of IYSV by a double-antibody sandwich (DAS)-ELISA (Agdia, Inc., Elkhart, IN). One of three nonsymptomatic spiny sowthistle samples tested positive by ELISA for IYSV. Total RNA was extracted from the leaf using the RNeasy Plant Mini Kit (Qiagen, Valencia, CA) following the manufacturer's protocol. Two microliters were used for reverse transcription (RT)-PCR with the forward primer (5′-TCAGAAATCGAGAAACTT-3′) and reverse primer (5′-TAATTATATCTATCTTTCTTGG-3′) for the IYSV nucleocapsid gene (1). A band of the expected size (approximately 800 bp) was obtained and sequenced. The sequence from the sowthistle (GenBank Accession No. EU078327) matched IYSV sequences from Georgia and Peru in a BLAST search in GenBank (closest matches with Accession Nos. DQ838584, DQ838592, DQ838593, and DQ658242). This is to our knowledge, the first confirmed report of IYSV infecting spiny sowthistle. The distribution of IYSV in sowthistle and its role as an oversummering host for IYSV is currently an on-going study. References: (1) L. du Toit et al. Plant Dis. 88:222, 2004. (2) D. H. Gent et al. Plant Dis. 90:1468, 2006. (3) S. W. Mullis et al. Plant Dis. 88:1285, 2004.

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Iris yellow spot virus on Shallot and Onion in France

Plant Health Progress ◽

10.1094/php-2008-0610-01-br ◽

2008 ◽

Vol 9 (1) ◽

pp. 46 ◽

Cited By ~ 7

Author(s):

Odile Huchette ◽

Christèle Bellamy ◽

Rodolphe Filomenko ◽

Blandine Pouleau ◽

Sam Seddas ◽

...

Keyword(s):

European Union ◽

The Netherlands ◽

Iris Yellow Spot Virus ◽

Yellow Spot ◽

The European Union ◽

Spot Virus ◽

Viral Pathogen ◽

The World ◽

Production Areas ◽

The Impact

Iris yellow spot virus is an economically important viral pathogen of onion. The virus has been reported from several countries in the world in recent years and, in Europe, the virus was reported from the Netherlands, Slovenia, Italy, and more recently from Spain. This is the first report of the virus in France, which ranks fifth in onion production in the European Union. The impact of the IYSV on onion and shallot in the different production areas of France remains to be seen. Accepted for publication 7 March 2008. Published 10 June 2008.

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