First Report of Squash vein yellowing virus in Watermelon in Guatemala

2015 ◽  
Vol 16 (3) ◽  
pp. 113-114 ◽  
Author(s):  
Ayyamperumal Jeyaprakash ◽  
Carlye A. Baker ◽  
Timothy S. Schubert ◽  
Ismael E. Badillo-Vargas ◽  
Pamela D. Roberts ◽  
...  
Keyword(s):  
Central America ◽  
First Report ◽  
Yellowing Virus

Watermelon and other cucurbits are important crops grown in Guatemala for local consumption and export. This is the first report of SqVYV infecting watermelon in Central America. Accepted for publication 14 July 2015. Published 15 July 2015.

scholarly journals Avian Botulism Type C in a Commercial Poultry Farm: First Report in Central America

2018 ◽  
Vol 09 (03) ◽  
Author(s):  
Evelyn Rodriguez Cavallini ◽  
Diana Lopez Urena ◽  
Tania Roman ◽  
Carlos Quesada Gomez
Keyword(s):  
Central America ◽  
Poultry Farm ◽  
First Report ◽  
Commercial Poultry ◽  
Type C ◽  
Avian Botulism

scholarly journals Squash vein yellowing virus Identified in Watermelon (Citrullus lanatus) in Indiana

Plant Disease ◽  
2007 ◽  
Vol 91 (8) ◽  
pp. 1056-1056 ◽  
Author(s):  
D. S. Egel ◽  
S. Adkins
Keyword(s):  
Citrullus Lanatus ◽  
Amino Acid Sequences ◽  
Rt Pcr ◽  
Strain Vector ◽  
First Report ◽  
Pcr Product ◽  
Field Samples ◽  
Health Progress ◽  
Vine Decline ◽  
Yellowing Virus

During September 2006, moderate vine decline symptoms including vine collapse and wilt and root rot were observed on numerous watermelon plants growing in a commercial field in Sullivan County, Indiana. No symptoms were observed on the fruit. Six plants displaying typical vine decline symptoms were collected and assayed for potyvirus infection and subsequently for Squash vein yellowing virus (SqVYV) and Papaya ringspot virus type W (PRSV-W). SqVYV is a whitefly-transmitted member of the Potyviridae, recently shown to cause watermelon vine decline in Florida (1,4). Plants infected with SqVYV in Florida are also frequently infected with PRSV-W, although SqVYV is sufficient for watermelon vine decline. The six field samples harbored one or more potyviruses as determined by ELISA (Agdia, Elkhart, IN). Mechanical inoculation of squash (Cucurbita pepo) and watermelon with sap from three of the field samples induced mosaic symptoms in both that are typical of potyviruses. Vein yellowing in squash and plant death in watermelon typical of SqVYV (1) later developed in plants inoculated with one field sample. A coat protein gene fragment was amplified by reverse transcription (RT)-PCR with SqVYV primers (1) from total RNA of five of the six field samples and also from the symptomatic, inoculated plants. Nucleotide and deduced amino acid sequences of a 957-bp region of the RT-PCR product (primer sequences deleted prior to analysis) were 100% identical to SqVYV (GenBank accession No. DQ812125). PRSV-W also was identified in two of the five SqVYV-infected field samples by ELISA (Agdia) and by sequence analysis of a 3′ genome fragment amplified by RT-PCR with previously described degenerate potyvirus primers (3). No evidence for infection by other potyviruses was obtained. To our knowledge, this is the first report of SqVYV in Indiana and the first report of the virus anywhere outside of Florida. The whitefly (Bemisia tabaci, B strain) vector of SqVYV is relatively uncommon in Indiana and the cold winter temperatures make it unlikely that any SqVYV-infected watermelon vines or whiteflies will overseason, necessitating reintroductions of virus and vector each season. We feel that the moderate and restricted occurrence of SqVYV in Indiana observed in September 2006 should pose little or no threat to commercial watermelon production in Indiana and should not cause growers to alter their growing practices. The occurrence of SqVYV in Indiana does not appear to explain the similar symptoms of mature watermelon vine decline (MWVD) that has been observed in Indiana since the 1980s. In contrast with the insect vectored SqVYV, MWVD seems to be caused by a soilborne biological agent (2). References: (1) S. Adkins et al. Phytopathology 97:145, 2007. (2) D. S. Egel et al. Online publication. doi:10.1094/PHP-2000-1227-01-HN. Plant Health Progress, 2000. (3) A. Gibbs and A. Mackenzie. J. Virol. Methods 63:9, 1997. (4) P. Roberts et al. Citrus Veg. Mag. December 12, 2004.

scholarly journals First Report of Squash vein yellowing virus Affecting Watermelon and Bitter Gourd in Puerto Rico

Plant Disease ◽  
2013 ◽  
Vol 97 (11) ◽  
pp. 1516-1516 ◽  
Author(s):  
V. Acevedo ◽  
J. C. V. Rodrigues ◽  
C. E. de Jensen ◽  
C. G. Webster ◽  
S. Adkins ◽  
...  
Keyword(s):  
Puerto Rico ◽  
Fungal Pathogens ◽  
Citrullus Lanatus ◽  
Direct Sequencing ◽  
Bitter Gourd ◽  
Growth Stages ◽  
Mechanical Inoculation ◽  
First Report ◽  
South Central ◽  
Yellowing Virus

In 2005, symptoms of watermelon vine decline (WVD) were observed on a 200-acre watermelon farm in Santa Isabel, on the south central coast of Puerto Rico. WVD symptoms included leaf curling, mosaics, and internode necrosis. In early growth stages of WVD, reduced vigor and plant stunting occurred. At flowering, symptoms progressed to necrosis and wilting of vines. A 2006 to 2007 survey demonstrated that fungal pathogens were not associated with the presence of WVD symptoms (3,4). By 2006, other watermelon fields were also affected. Field trials in 2007 and 2008 with insect-proof cages and insecticides suggested a role of whiteflies (Bemisia tabaci) in the transmission of a virus (3,4). Here, we report that watermelon and pumpkin plants were successfully infected in Puerto Rico by mechanical inoculation and through B. tabaci transmission assays, similarly to transmissions previously conducted in Florida with Squash vein yellowing virus (SqVYV) (1). In addition, plants of Cucurbita moschata exhibited vein clearing symptoms typical of SqVYV after mechanical inoculation with extracts from watermelon plants with WVD symptoms. In 2011, eight watermelon samples from plants exhibiting WVD syndrome were collected in Guánica, Santa Isabel, Juana Díaz, and Mayagüez, and two Momordica charantia samples were collected from Mayagüez. RNA was extracted from all 10 original samples, as well as from plants that were used in mechanical and vector transmission assays, using RNeasy Plant Mini Kit (Qiagen, Valencia, California), and all samples were found positive for SqVYV by reverse transcription-PCR, using previously described primers and methods (1,2). In all cases, a single ~1-kb PCR fragment was revealed, and PCR fragments from four samples were selected for direct sequencing. All sequences showed high levels (>99%) of nucleotide identity with SqVYV sequences from Florida (JF897989, JF897985, and JF897984). Sequences of the SqVYV CP gene from Puerto Rico were deposited in GenBank under accession numbers KC713961 to KC713964. To our knowledge, this is the first report of SqVYV in Puerto Rico associated with WVD syndrome in cucurbits, and thus has implications for management of viral diseases of watermelon in the Caribbean. This is also the first detection of SqVYV outside of the continental United States in both watermelon and a wild species, M. charantia (bitter gourd). References: (1) S. Adkins et al. Phytopathology 97:145, 2007. (2) S. Adkins et al. Plant Dis. 92:1119, 2008. (3) C. Estévez de Jensen et al. Phytopathology 98:S52, 2008. (4) L. Polanco-Florián. El marchitamiento súbito de la sandía [Citrullus lanatus (Thumb.) Matsum & Nakai]. M.S. thesis, University of Puerto Rico, Mayagüez, PR, 2009.

scholarly journals First report of naturalization of Houttuynia cordata Thunb. 1783 (Piperales: Saururaceae) in South America

Check List ◽  
2013 ◽  
Vol 9 (3) ◽  
pp. 642 ◽  
Author(s):  
Ruy José Válka Alves ◽  
Débora Medeiros ◽  
Ricardo Loyola de Moura ◽  
Luiza Carla Trindade de Gusmão ◽  
Nílber Gonçalves da Silva ◽  
...  
Keyword(s):  
Costa Rica ◽  
South America ◽  
Central America ◽  
National Park ◽  
First Record ◽  
Houttuynia Cordata ◽  
First Report ◽  
Houttuynia Cordata Thunb ◽  
Established Population

A relatively large and established population of Houttuynia cordata from Itatiaia National Park in Brazil represents the first record of naturalized Saururaceae in South America. Although the species is potentially invasive, unknown mechanisms have prevented its spread to other localities between 1940, when it was recorded in cultivation in Brazil, and the present. The nearest known naturalized population is situated 5,600 km away, in Costa Rica, Central America.

First Report of Macrophomina phaseolina, Fusarium brachygibbosum, and Lasiodiplodia theobromae Causing Fungal Watermelon Vine Decline in Southwest and West-Central Florida

2021 ◽  
Author(s):  
Cristina Pisani ◽  
Scott Adkins ◽  
William W Turechek ◽  
Pragna C Patel ◽  
Erin Rosskopf
Keyword(s):  
Disease Severity ◽  
Fungal Pathogens ◽  
Disease Incidence ◽  
Macrophomina Phaseolina ◽  
Lasiodiplodia Theobromae ◽  
First Report ◽  
Central Florida ◽  
Untreated Check ◽  
Vine Decline ◽  
Yellowing Virus

Wilt and vine decline symptoms were observed on watermelon plants in Glades and Hardee Counties in Florida in spring 2017 that resembled viral watermelon vine decline caused by squash vein yellowing virus (SqVYV). When no SqVYV was detected, greenhouse studies and morphological and molecular analyses revealed three fungal pathogens, Macrophomina phaseolina, Fusarium brachygibbosum, and Lasiodiplodia theobromae, that were not previously reported on watermelon in Florida. A previously reported oomycete, Pythium spinosum, was also detected in some, but not all isolates, and but when applied independently, resulted in disease incidence that was comparable to the untreated check, ruling it out as a primary causal agent of the symptoms observed in the field. In one of three experiments, seedlings inoculated with a combination of Macrophomina phaseolina, Fusarium brachygibbosum, and Pythium spinosum suffered the highest disease severity based on AUDPC values. In another experiment, seedlings inoculated with F. brachygibbosum exhibited the most severe symptoms and rapid disease development following inoculation. When seeds were inoculated with either a single or a combination of the isolated fungi, those inoculated with L. theobromae resulted in seedlings with the greatest disease severity. This is the first report of these three fungal pathogens on watermelon in Florida.

scholarly journals A molecular study of Neophyllaphis varicolor (Hemiptera, Aphididae) in Costa Rica

ZooKeys ◽  
2019 ◽  
Vol 865 ◽  
pp. 123-135
Author(s):  
Adonay Zúñiga-Centeno ◽  
Izayana Sandoval-Carvajal ◽  
Mauricio Montero-Astúa ◽  
William Villalobos-Muller ◽  
Nguyễn Bảo Quốc ◽  
...  
Keyword(s):  
New Species ◽  
Costa Rica ◽  
Central America ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Molecular Study ◽  
First Report ◽  
Factor I ◽  
A New Species

The genus Neophyllaphis (Takahashi) (Aphididae: Neophyllaphidinae) is composed of 18 species; however, in the Americas only nine species have been reported previously. A new species, Neophyllaphisvaricolor Miller & Halbert, was described in 2014 in USA. Colonies resembling those of this new species have been observed in Costa Rica on Podocarpus spp. In order to determine if N.varicolor is also present in Costa Rica, we sampled Neophyllaphis colonies from Podocarpusfalcatus and P.chinensis. Additionally, we sampled individuals from Podocarpus sp. in Spain and Vietnam. DNA of each sample was extracted and used to amplify and sequence the cytochrome c oxidase subunit I (COI) and elongation factor I (EF-1α) partial regions. According to morphological characteristics, sequences comparisons done in GenBank and BOLD, and phylogenetic analyses, the colonies collected from Podocarpus spp. in Costa Rica and the colony from Vietnam corresponded to the species N.varicolor. To the best of our knowledge this is the first report of the presence of N.varicolor in Central America and Vietnam.

scholarly journals First report for the Neotropics of predation by the waterscorpion Ranatra (Hemiptera: Nepidae) on a member of Gerromorpha (Hemiptera)

2013 ◽  
Vol 5 (1) ◽  
pp. 55-56
Author(s):  
Federico Herrera
Keyword(s):  
Costa Rica ◽  
Central America ◽  
Laboratory System ◽  
Water Strider ◽  
First Report ◽  
Closed Laboratory

The water scorpion Ranatra pittieri was observed preying on an adult of the water strider Eurygerris flavolineatus in a closed laboratory system. This is the first report for the Neotropics of the predation by a member of the infraorder Nepomorpha on a species of Gerromorpha.KEYWORDWaterscorpions, Heteroptera, Gerridae, Nepomorpha, Costa Rica, Central America

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