Evaluation of the phytotoxicity of 2,4-diacetylphloroglucinol and Pseudomonas brassicacearum Q8r1-96 on different wheat cultivars

2021 ◽  
Author(s):  
Mingming Yang ◽  
Linda S Thomashow ◽  
David M Weller
Keyword(s):  
Seed Germination ◽  
Pacific Northwest ◽  
Root Colonization ◽  
The United States ◽  
Wheat Cultivars ◽  
The Pacific ◽  
Root Necrosis ◽  
Growth Promoting ◽  
Pseudomonas Brassicacearum

Pseudomonas brassicacearum Q8r1-96 and other 2,4-diacetylphloroglucinol (DAPG)-producing pseudomonads of the Pseudomonas fluorescens complex possess both biocontrol and growth-promoting properties and play an important role in suppression of take-all of wheat in the Pacific Northwest (PNW) of the United States. However, P. brassicacearum can also reduce seed germination and cause root necrosis on some wheat cultivars. We evaluated the effect of Q8r1-96 and DAPG on the germination of 69 wheat cultivars that have been or currently are grown in the PNW. Cultivars varied widely in their ability to tolerate P. brassicacearum or DAPG. The frequency of germination of the cultivars ranged from 0 to 0.87 and from 0.47 to 0.90 when treated with Q8r1-96 and DAPG, respectively. There was a significant positive correlation between the frequency of germination of cultivars treated with Q8r1-96 in assays conducted in vitro and in the greenhouse. The correlation was greater for spring than for winter cultivars. In contrast, the effect of Q8r1-96 on seed germination was not correlated with that of DAPG alone, suggesting that DAPG is not the only factor responsible for the phytotoxicity of Q8r1-96. Three wheat cultivars with the greatest tolerance and three cultivars with the least tolerance to Q8r1-96 were tested for their ability to support root colonization by strain Q8r1-96. Cultivars with the greatest tolerance supported significantly greater populations of strain Q8r1-96 than those with the least tolerance to the bacteria. Our results show that wheat cultivars differ widely in their interaction with P. brassicacearum and the biocontrol antibiotic DAPG.

scholarly journals Correlation of Genotype and In Vitro Susceptibilities of Cryptococcus gattii Strains from the Pacific Northwest of the United States

2009 ◽  
Vol 48 (2) ◽  
pp. 539-544 ◽  
Author(s):  
N. Iqbal ◽  
E. E. DeBess ◽  
R. Wohrle ◽  
B. Sun ◽  
R. J. Nett ◽  
...  
Keyword(s):  
United States ◽  
Pacific Northwest ◽  
The United States ◽  
Cryptococcus Gattii ◽  
The Pacific

scholarly journals Differential Response of Wheat Cultivars to Pseudomonas brassicacearum and Take-All Decline Soil

2018 ◽  
Vol 108 (12) ◽  
pp. 1363-1372 ◽  
Author(s):  
Mingming Yang ◽  
Dmitri V. Mavrodi ◽  
Linda S. Thomashow ◽  
David M. Weller
Keyword(s):  
United States ◽  
Pacific Northwest ◽  
Seedling Emergence ◽  
The United States ◽  
Wheat Cultivars ◽  
Control Growth ◽  
The Pacific ◽  
Rhizosphere Population ◽  
Population Sizes ◽  
Take All

2,4-Diacetylphloroglucinol (DAPG)-producing Pseudomonas spp. in the P. fluorescens complex are primarily responsible for a natural suppression of take-all of wheat known as take-all decline (TAD) in many fields in the United States. P. brassicacearum, the most common DAPG producer found in TAD soils in the Pacific Northwest (PNW) of the United States, has biological control, growth promoting and phytotoxic activities. In this study, we explored how the wheat cultivar affects the level of take-all suppression when grown in a TAD soil, and how cultivars respond to colonization by P. brassicacearum. Three cultivars (Tara, Finley, and Buchanan) supported similar rhizosphere population sizes of P. brassicacearum when grown in a TAD soil, however they developed significantly different amounts of take-all. Cultivars Tara and Buchanan developed the least and most take-all, respectively, and Finley showed an intermediate amount of disease. However, when grown in TAD soil that was pasteurized to eliminate both DAPG producers and take-all suppression, all three cultivars were equally susceptible to take-all. The three cultivars also responded differently to the colonization and phytotoxicity of P. brassicacearum strains Q8r1-96 and L5.1-96, which are characteristic of DAPG producers in PNW TAD soils. Compared with cultivar Tara, cultivar Buchanan showed significantly reduced seedling emergence and root growth when colonized by P. brassicacearum, and the response of Finley was intermediate. However, all cultivars emerged equally when treated with a DAPG-deficient mutant of Q8r1-96. Our results indicate that wheat cultivars grown in a TAD soil modulate both the robustness of take-all suppression and the potential phytotoxicity of the antibiotic DAPG.

scholarly journals A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sujit Shah ◽  
Krishna Chand ◽  
Bhagwan Rekadwad ◽  
Yogesh S. Shouche ◽  
Jyotsna Sharma ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root Colonization ◽  
Endophytic Bacterium ◽  
Epifluorescence Microscopy ◽  
In Vitro Cultivation ◽  
Rrna Gene ◽  
Bacillus Spp ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background A plant growth-promoting endophytic bacterium PVL1 isolated from the leaf of Vanda cristata has the ability to colonize with roots of plants and protect the plant. PVL1 was isolated using laboratory synthetic media. 16S rRNA gene sequencing method has been employed for identification before and after root colonization ability. Results Original isolated and remunerated strain from colonized roots were identified as Bacillus spp. as per EzBiocloud database. The presence of bacteria in the root section of the plantlet was confirmed through Epifluorescence microscopy of colonized roots. The in-vitro plantlet colonized by PVL1 as well as DLMB attained higher growth than the control. PVL1 capable of producing plant beneficial phytohormone under in vitro cultivation. HPLC and GC-MS analysis suggest that colonized plants contain Indole Acetic Acid (IAA). The methanol extract of Bacillus spp., contains 0.015 μg in 1 μl concentration of IAA. PVL1 has the ability to produce antimicrobial compounds such as ethyl iso-allocholate, which exhibits immune restoring property. One-way ANOVA shows that results were statistically significant at P ≤ 0.05 level. Conclusions Hence, it has been concluded that Bacillus spp. PVL1 can promote plant growth through secretion of IAA during root colonization and ethyl iso-allocholate to protect plants from foreign infections. Thus, this study supports to support Koch’s postulates of bacteria establishment.

scholarly journals First record of Euxestonotus error (Hymenoptera: Platygastridae) in the Pacific Northwest, United States of America

2016 ◽  
Vol 148 (5) ◽  
pp. 616-618 ◽  
Author(s):  
E.R. Echegaray ◽  
R.N. Stougaard ◽  
B. Bohannon
Keyword(s):  
United States ◽  
New York ◽  
Pacific Northwest ◽  
United States Of America ◽  
First Record ◽  
The United States ◽  
Sitodiplosis Mosellana ◽  
Wheat Midge ◽  
The Pacific ◽  
Northwestern Montana

AbstractEuxestonotus error (Fitch) (Hymenoptera: Platygastridae) is considered part of the natural enemy complex of the wheat midge Sitodiplosis mosellana (Géhin) (Diptera: Cecidomyiidae). Although previously reported in the United States of America, there is no record for this species outside the state of New York since 1865. A survey conducted in the summer of 2015 revealed that E. error is present in northwestern Montana and is likely playing a role in the suppression of wheat midge populations.

scholarly journals Erysiphe trifolii Causing Powdery Mildew of Lentil (Lens culinaris)

Plant Disease ◽  
2009 ◽  
Vol 93 (8) ◽  
pp. 797-803 ◽  
Author(s):  
Renuka N. Attanayake ◽  
Dean A. Glawe ◽  
Frank M. Dugan ◽  
Weidong Chen
Keyword(s):  
Powdery Mildew ◽  
Pacific Northwest ◽  
The United States ◽  
Morphological Characters ◽  
Grain Legumes ◽  
Its Sequences ◽  
Powdery Mildew Fungus ◽  
The Pacific ◽  
Soybean Genotypes ◽  
Erysiphe Trifolii

The taxonomy of the powdery mildew fungus infecting lentil in the Pacific Northwest (PNW) of the United States was investigated on the basis of morphology and rDNA internal transcribed spacer (ITS) sequences. Anamorphic characters were in close agreement with descriptions of Erysiphe trifolii. However, teleomorphs formed chasmothecial appendages with highly branched apices, whereas E. trifolii has been described as producing flexuous or sometimes loosely branched appendages. Branched appendages have been described in Erysiphe diffusa, a fungus reported from species of Lens, Glycine, and Sophora, raising the possibility that the PNW fungus could be E. diffusa. Examination of morphological characters of an authentic specimen of E. trifolii from Austria determined that it included chasmothecial appendages resembling those seen in PNW specimens. Furthermore, ITS sequences from five powdery mildew samples collected from lentils in PNW greenhouses and fields from 2006 to 2008 were identical to one another, and exhibited higher similarity to sequences of E. trifolii (99%) than to those of any other Erysiphe spp. available in GenBank. Parsimony analysis grouped the lentil powdery mildew into a clade with Erysiphe baeumleri, E. trifolii, and E. trifolii–like Oidium sp., but indicated a more distant relationship to E. diffusa. In greenhouse inoculation studies, the lentil powdery mildew fungus did not infect soybean genotypes known to be susceptible to E. diffusa. The pathogenicity of E. trifolii on lentil was confirmed using modified Koch's postulates. This is the first report of E. trifolii infecting lentil. E. diffusa and E. trifolii have different host ranges, so the discovery of E. trifolii on lentil has implications both for determining species of powdery mildews on cool-season grain legumes, and in disease management.

scholarly journals If You Provide, Will They Ride? Motivators and Deterrents to Shared Micro-Mobility

2020 ◽  
pp. 26-38
Author(s):  
Ronald W. Pimentel, Ph.D. ◽  
Michael B. Lowry, Ph.D. ◽  
David Pimentel, J.D. ◽  
Amanda K. Glazer ◽  
Timothy W. Koglin ◽  
...  
Keyword(s):  
Pacific Northwest ◽  
Reasoned Action ◽  
Secondary Data ◽  
The United States ◽  
The Pacific ◽  
Micro Mobility ◽  
On Line ◽  
Optimal System Design ◽  
Depth Interviews ◽  
Bike Share

Bike share, e-bike share, and e-scooter systems (shared micro-mobility) are gaining popularity throughout the United States and internationally, but the optimal system design has not been determined. This study investigated motivators and deterrents to the use of such systems in the Pacific Northwest with secondary data, participant observations, depth interviews, and an on-line survey to users and non-users. The survey was administered in all cities in Washington, Oregon, and Idaho that have shared micro-mobility systems. The strongest motivators reported were exercise and enjoyment. The strongest deterrents were weather, danger from automobile traffic, and insufficient bike lanes and paths. The latter two deterrents might be alleviated through continued improvements to infrastructure; however, the weather cannot be changed, and neither can hills. Data were fitted to the Theory of Reasoned Action and the resulting recommendation is to promote popular motivators of exercise and enjoyment and emphasize personal benefits more than social appearances.

“How Can We Catch Fire?”

2021 ◽  
pp. 119-143
Author(s):  
Melanie C. Ross
Keyword(s):  
United States ◽  
African American ◽  
Pacific Northwest ◽  
Holy Spirit ◽  
The United States ◽  
Kingdom Of God ◽  
The Pacific ◽  
The Holy Spirit ◽  
Influence At Work ◽  
Vineyard Movement

Chapter 5 explores the Vineyard movement, one of the fastest-growing church movements in the United States, which is committed to holding together the “already” and “not yet” of the Kingdom of God in worship. In addition to looking for a dramatic, miraculous inbreaking of the Holy Spirit, there is a less dramatic but equally formative influence at work in worship: the Quaker notion of “gospel order” and its accompanying understanding of ethics. These commitments are tested at “Koinonia Vineyard,” a congregation located in the Pacific Northwest, where one African American member wrestles with her vision of activism and her Caucasian pastor’s desire for the congregation to remain politically neutral during a time of national racial unrest.

Understanding Decision Making by Families About Youth Football Participation Postconcussion

2020 ◽  
pp. 152483992094525
Author(s):  
Emily Kroshus ◽  
Deborah Bowen ◽  
Douglas Opel ◽  
Sara P. D. Chrisman ◽  
Frederick P. Rivara
Keyword(s):  
Pacific Northwest ◽  
Decision Aid ◽  
Sports Participation ◽  
The United States ◽  
Football Players ◽  
Contact Sport ◽  
Sample Mean ◽  
Youth Football ◽  
The Pacific ◽  
Northwest Region

Many families are concerned about their child’s risk of concussion, and some seek counsel from clinicians about whether or not to return to contact sports participation postinjury. The present study sought to identify factors that parents weight most heavily in forming their preferences regarding whether their child should return to contact sport after recovering from a concussion. Survey data were collected from 568 parents of youth football players (aged 7–14 years) in the Pacific Northwest region of the United States (73% response rate). Approximately two thirds (63%) of parents preferred that their child retire from football after one or two concussions. Multivariable linear regression indicated parents above the sample mean in terms of how strongly they valued football participation preferred their child stop after more concussions than parents below the sample mean (β = .44, standard error [SE] = 0.06, p < .001). Factors endorsed by the most parents as making them “much more likely” to want their child to stop playing football included the belief that their child will experience cognitive issues later in life as a result of concussions (65.0%) and that their child will get another concussion while playing football (43.5%). Within the context of a clinical visit postconcussion, physicians may need to help families clarify their values related to football participation and provide information about the potential outcomes of returning to contact sport. A formalized shared decision aid could help support consistent implementation of this potentially challenging conversation.

scholarly journals Climate Politics and Race in the Pacific Northwest

2018 ◽  
Vol 7 (10) ◽  
pp. 192 ◽  
Author(s):  
Rachel Slocum
Keyword(s):  
Pacific Northwest ◽  
The United States ◽  
Climate Justice ◽  
Structural Racism ◽  
Systemic Racism ◽  
Climate Politics ◽  
The Public ◽  
Racial Capitalism ◽  
The Pacific ◽  
Global North

The collective politics of climate justice makes the important claim that lowering emissions is not enough; society must also undertake radical transformation to address both the climate and inequality crises. Owing to its roots in the environmental justice movement, addressing systemic racism is central to climate justice praxis in the United States, which is a necessary intervention in typically technocratic climate politics. What emerges from US climate justice is a moral appeal to ‘relationship’ as politics, the procedural demand that communities of color (the ‘frontline’) lead the movement, and a distributive claim on carbon pricing revenue. However, this praxis precludes a critique of racial capitalism, the process that relies on structural racism to enhance accumulation, alienating, exploiting, and immiserating black, brown, and white, while carrying out ecocide. The lack of an analysis of how class and race produce the crises climate justice confronts prevents the movement from demanding that global north fossil fuel abolition occur in tandem with the reassertion of the public over the private and de-growth. Drawing on research conducted primarily in Oregon and Washington, I argue that race works to both create and limit the transformative possibilities of climate politics.

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