scholarly journals Geographic Distribution of Cryptic Species of Plasmopara viticola Causing Downy Mildew on Wild and Cultivated Grape in Eastern North America

2014 ◽  
Vol 104 (7) ◽  
pp. 692-701 ◽  
Author(s):  
Mélanie Rouxel ◽  
Pere Mestre ◽  
Anton Baudoin ◽  
Odile Carisse ◽  
Laurent Delière ◽  
...  

The putative center of origin of Plasmopara viticola, the causal agent of grape downy mildew, is eastern North America, where it has been described on several members of the family Vitaceae (e.g., Vitis spp., Parthenocissus spp., and Ampelopsis spp.). We have completed the first large-scale sampling of P. viticola isolates across a range of wild and cultivated host species distributed throughout the above region. Sequencing results of four partial genes indicated the presence of a new P. viticola species on Vitis vulpina in Virginia, adding to the four cryptic species of P. viticola recently recorded. The phylogenetic analysis also indicated that the P. viticola species found on Parthenocissus quinquefolia in North America is identical to Plasmopara muralis in Europe. The geographic distribution and host range of five pathogen species was determined through analysis of the internal transcribed spacer polymorphism of 896 isolates of P. viticola. Among three P. viticola species found on cultivated grape, one was restricted to Vitis interspecific hybrids within the northern part of eastern North America. A second species was recovered from V. vinifera and V. labrusca, and was distributed across most of the sampled region. A third species, although less abundant, was distributed across a larger geographical range, including the southern part of eastern North America. P. viticola clade aestivalis predominated (83% of isolates) in vineyards of the European winegrape V. vinifera within the sampled area, indicating that a single pathogen species may represent the primary threat to the European host species within eastern North America.

2018 ◽  
Vol 19 (2) ◽  
pp. 139-139 ◽  
Author(s):  
Xuewen Feng ◽  
Anton Baudoin

This report documents the first known occurrence in North America of resistance in grape downy mildew (Plasmopara viticola) to the carboxylic acid amide (CAA) fungicides mandipropamid and dimethomorph. These fungicides (FRAC group 40) have been an important component of downy mildew management programs for the past decade. Resistant isolates were obtained at three locations in Virginia and one in North Carolina, at considerable distances from each other. Resistance was documented by bioassay and the presence of the G1105S mutation, which has been associated with CAA resistance of P. viticola in other areas. Further survey is needed to determine the geographic extent of this resistance.


Plant Disease ◽  
2000 ◽  
Vol 84 (5) ◽  
pp. 549-554 ◽  
Author(s):  
L. V. Madden ◽  
M. A. Ellis ◽  
N. Lalancette ◽  
G. Hughes ◽  
L. L. Wilson

An electronic warning system for grape downy mildew— based on models for the infection of leaves of Vitis lambrusca, production of sporangia by Plasmopara viticola in lesions, and sporangial survival—was tested over 7 years in Ohio. Grapevines were sprayed with metalaxyl plus mancozeb (Ridomil MZ58) when the warning system indicated that environmental conditions were favorable for sporulation and subsequent infection. Over the 7 years, plots were sprayed from one to four times according to the warning system, and from four to 10 times according to the standard calendar-based schedule (depending on the date of the initiation of the experiment). The warning system resulted in yearly reductions of one to six sprays (with median of three sprays). Disease incidence (i.e., proportion of leaves with symptoms) in unsprayed plots at the end of the season ranged from 0 to 86%, with a median of 68%. Incidence generally was very similar for the warning-system and standard-schedule treatments (median of 7% of the leaves with symptoms), and both of these incidence values were significantly lower (P < 0.05) than that found for the unsprayed control, based on a generalized-linear-model analysis. Simplifications of the disease warning system, where sprays were applied based only on the infection or sporulation components of the system, were also effective in controlling the disease, although more fungicide applications sometimes were applied. Effective control of downy mildew, therefore, can be achieved with the use of the warning system with fewer sprays than a with a standard schedule.


Author(s):  
Kandace D. Hollenbach ◽  
Stephen B. Carmody

The possibility that native peoples in eastern North America had cultivated plants prior to the introduction of maize was first raised in 1924. Scant evidence was available to support this speculation, however, until the “flotation revolution” of the 1960s and 1970s. As archaeologists involved in large-scale projects began implementing flotation, paleoethnobotanists soon had hundreds of samples and thousands of seeds that demonstrated that indigenous peoples grew a suite of crops, including cucurbit squashes and gourds, sunflower, sumpweed, and chenopod, which displayed signs of domestication. The application of accelerator mass spectrometry (AMS) dating to cucurbit rinds and seeds in the 1980s placed the domestication of these four crops in the Late Archaic period 5000–3800 bp. The presence of wild cucurbits during earlier Archaic periods lent weight to the argument that native peoples in eastern North America domesticated these plants independently of early cultivators in Mesoamerica. Analyses of DNA from chenopods and cucurbits in the 2010s definitively demonstrated that these crops developed from local lineages. With evidence in hand that refuted notions of the diffusion of plant domestication from Mesoamerica, models developed in the 1980s for the transition from foraging to farming in the Eastern Woodlands emphasized the coevolutionary relationship between people and these crop plants. As Archaic-period groups began to occupy river valleys more intensively, in part due to changing climatic patterns during the mid-Holocene that created more stable river systems, their activities created disturbed areas in which these weedy plants thrive. With these useful plants available as more productive stands in closer proximity to base camps, people increasingly used the plants, which in turn responded to people’s selection. Critics noted that these models left little room for intentionality or innovation on the part of early farmers. Models derived from human behavioral ecology explore the circumstances in which foragers choose to start using these small-seeded plants in greater quantities. In contrast to the resource-rich valley settings of the coevolutionary models, human behavioral ecology models posit that foragers would only use these plants, which provide relatively few calories per time spent obtaining them, when existing resources could no longer support growing populations. In these scenarios, Late Archaic peoples cultivated these crops as insurance against shortages in nut supplies. Despite their apparent differences, current iterations of both models recognize humans as agents who actively change their environments, with intentional and unintentional results. Both also are concerned with understanding the social and ecological contexts within which people began cultivating and eventually domesticating plants. The “when” and “where” questions of domestication in eastern North America are relatively well established, although researchers continue to fill significant gaps in geographic data. These primarily include regions where large-scale contract archaeology projects have not been conducted. Researchers are also actively debating the “how” and “why” of domestication, but the cultural ramifications of the transition from foraging to farming have yet to be meaningfully incorporated into the archaeological understanding of the region. The significance of these native crops to the economies of Late Archaic and subsequent Early and Middle Woodland peoples is poorly understood and often woefully underestimated by researchers. The socioeconomic roles of these native crops to past peoples, as well as the possibilities for farmers and cooks to incorporate them into their practices in the early 21st century, are exciting areas for new research.


2007 ◽  
Vol 58 (7) ◽  
pp. 702 ◽  
Author(s):  
M. G. Williams ◽  
P. A. Magarey ◽  
K. Sivasithamparam

Plasmopara viticola, causal agent of grape downy mildew, was first detected in Western Australia (WA) in widespread commercial viticulture in the Swan Valley (–31.85 S, 116.02 E) in 1998. It has since been found in all viticultural areas in WA, which extend from the far north (–15.75 S, 128.74 E) to the far south (–35.02 S, 117.80 E) of the state across a diverse range of climate zones. Not all of these zones are considered conducive for the development of grape downy mildew. The early infection behaviour of P. viticola isolates, obtained from climatically different grape-growing locations, was examined under different temperatures of incubation (10, 20, and 30°C). Variation in early infection behaviour was used to discern ecotypes of the pathogen. Ten isolates were obtained from WA and 8 of these responded similarly. Three isolates obtained from the east of Australia and used as comparisons in the study behaved similarly to most of the WA isolates. Zoospore germination and host penetration occurred at 10 and 20°C for all isolates. None of the isolates penetrated the host at 30°C. Only 2 isolates, from WA, germinated at 30°C. The early infection behaviour of most of the P. viticola isolates examined in this study appears to be reasonably analogous, despite the influence of varied local environmental conditions. The capacity of certain isolates to germinate under a wider spectrum of temperatures could be indicative of ecotypic specialisation. Such behaviour could confer advantage for the pathogen in viticultural regions that experience high temperatures throughout the growing season.


2008 ◽  
Vol 65 (spe) ◽  
pp. 60-64 ◽  
Author(s):  
Emília Hamada ◽  
Raquel Ghini ◽  
Paulo Rossi ◽  
Mário José Pedro Júnior ◽  
Jeferson Lobato Fernandes

Viticulture in Brazil has been growing in importance in recent years. In the State of São Paulo, a significant percentage of the production is basically destined to in natura consumption and, more recently, much effort has been made by institutions to revitalize the viticulture in the State. Among fungal diseases, the downy mildew (Plasmopara viticola) is one of the main diseases affecting this crop in Brazil, with extreme damage effects on its production. The objective of this study was to estimate the incidence of the downy mildew on grape under the climatic conditions of the State of São Paulo, based on a mathematical model and using Geographical Information System - GIS tools. The study considered the months from September to April, a period in which the downy mildew can affect grapevines under development. Mean temperature and relative humidity were the basic weather data entered in the GIS database. Leaf wetness duration was estimated from relative humidity measurements. Climatic data entered in the GIS were used to calculate and produce maps depicting the severity of the grape downy mildew, through the application of a disease model. Three cities were evaluated (Jales, Jundiaí, and São Miguel Arcanjo), since they represent the main vineyard centers in the State. The adopted methodology permitted quantifying the severity of the grape downy mildew not only in spatial terms, identifying the variability among the different regions of the State, but also in temporal terms, along the months, making an adequate distinction of the studied cities.


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