A Single Dominant Gene in Common Bean Conferring Resistance to Three Root-Knot Nematode Species

Tomato yellow leaf curl virus (TYLCV) and Tomato yellow leaf curl Málaga virus are monopartite begomoviruses (genus Begomovirus, family Geminiviridae) that infect common bean (Phaseolus vulgaris), causing bean leaf crumple disease (BLCD). This disease was found to be widespread in southern Spain and causes stunted growth, flower abortion, and leaf and pod deformation in common bean plants. Commercial yield losses of up to 100% occur. In the present study, we have identified and characterized a resistance trait to BLCD-associated viruses in the common bean breeding line GG12. This resistance resulted in a complete absence of BLCD symptoms under field conditions or after experimental inoculation. Our analysis showed that virus replication was not inhibited. However, a severe restriction to systemic virus accumulation occurred in resistant plants, suggesting that cell-to-cell or long-distance movement were impaired. In addition, recovery from virus infection was observed in resistant plants. The reaction of P. vulgaris lines GG12 (resistant) and GG14 (susceptible), and of F1, F2, and backcross populations derived from them, to TYLCV inoculation suggested that a single dominant gene conferred the BLCD resistance described here.

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Varying abundance and dispersal of the two-spotted spider mite (Tetranychus urticae Koch, 1836, Acari: Prostigmata: Tetranychidae) on Mi-tomato plants differing in allelic combination

Biological Letters ◽

10.2478/v10120-011-0022-8 ◽

2011 ◽

Vol 48 (2) ◽

pp. 213-223 ◽

Cited By ~ 1

Author(s):

Monika Godzina ◽

Małgorzata Kiełkiewicz ◽

Katarzyna Szymczykiewicz

Keyword(s):

Tetranychus Urticae ◽

Spider Mite ◽

Dominant Gene ◽

Nematode Species ◽

Spider Mites ◽

Root Knot Nematode ◽

Tomato Plants ◽

Allelic Combination ◽

Two Spotted Spider Mite ◽

Abundance And Distribution

Varying abundance and dispersal of the two-spotted spider mite (Tetranychus urticaeKoch, 1836, Acari: Prostigmata: Tetranychidae) onMi-tomato plants differing in allelic combinationIt is well known that the presence of theMi-1.2gene determines the resistance of tomato plants (Solanum lycopersicumL.) to 3 root-knot nematode species and some insect herbivores of the order Hemiptera. In this study, the density and dispersal of two-spotted spider mites (Tetranychus urticae) on tomato plants of 4 cultivars were evaluated under greenhouse conditions, to find out whether tomatoes differing in allelic combination of theMi-1.2gene exhibit similar or different resistance to the mite pest. The results show, for the first time, that the same initial number of mites develops into populations that vary in abundance and distribution depending on tomato allelic composition of theMi-1.2gene. The results indicate that the mite-pest develops more slowly on tomato plants of cultivar ‘Motelle’, carrying 2 dominant alleles for theMi-1.2gene, than on heterozygous ‘Altess F1’ tomatoes and both cultivars carrying only recessive alleles of this gene: ‘Moneymaker’ and ‘Plaisence F1’. This suggests that theMi-1.2gene may be involved in tomato resistance against spider mites when this dominant gene is expressed homozygously, but this needs to be verified by further research.

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First Report of the Root Knot Nematode, Meloidogyne inornata, on Common Bean in Paraná State, Brazil

Plant Disease ◽

10.1094/pdis-09-12-0832-pdn ◽

2013 ◽

Vol 97 (3) ◽

pp. 431-431 ◽

Cited By ~ 3

Author(s):

A. C. Z. Machado ◽

O. F. Dorigo ◽

D. Mattei

Keyword(s):

Common Bean ◽

Nematode Species ◽

State University ◽

Root Knot Nematode ◽

First Report ◽

North Carolina State University ◽

Adult Females ◽

Bean Plants ◽

Meloidogyne Spp ◽

Species Specific

Common bean (Phaseolus vulgaris F.) is one of the most important crops in Paraná State, which is responsible for almost 10% of the Brazilian production (4). Root knot nematodes, Meloidogyne spp., are common parasites of this crop worldwide, but damage caused by Meloidogyne inornata has not been reported. During a survey of nematode species present on common bean fields in Paraná State, Brazil, galled root samples of cultivars Tuiuiú and Eldorado were submitted, in June 2012, in the Nematology Laboratory from IAPAR, collected in the municipalities of Araucária (25°35′34″S, 49°24′36″W) and Santana do Itararé (23°45′18″S, 49°37′44″W). Plants did not exhibit any above-ground symptoms. The specimens were identified through perineal patterns and esterase phenotypes of 20 adult females extracted from dissected roots (2,3). The population densities observed in the samples were 140 and 700 J2 and eggs per gram of roots, respectively, for both samples. Characteristics were consistent with those described for M. inornata. For example, perineal patterns of M. inornata showed a high dorsal arch, with smooth to wavy striae, similar to those of M. incognita; but no punctate markings between anus and tail terminus were observed. However, from the esterase electrophoresis we obtained the I3 (Rm = 0.83, 1.15, and 1.32) phenotype, typical of M. inornata, a species-specific phenotype used to differentiate this species from M. incognita (1). Moreover, the excretory pore of adult females was located 32.1 (± 5.4) μm from the anterior end, consistent with the M. inornata description (25 to 53 μm) (1). To the best of our knowledge, this is the first report of M. inornata parasitizing common bean roots. This finding has great importance for Brazilian agriculture, since this nematode may damage common bean plants and become an additional problem for this crop. Additional work is necessary in order to elucidate the losses caused by M. inornata on common bean. References: (1) R. M. D. G. Carneiro et al. Nematology 10:123, 2008. (2) P. R. Esbenshade and A. C. Triantaphyllou J. Nematol. 22:10, 1990. (3) K. M. Hartman and J. N. Sasser. Page 115 in: An Advanced Treatise on Meloidogyne, Volume II Methodology. K. R. Barker et al., eds. Raleigh: North Carolina State University Graphics, 1985. (4) MAPA. Feijão, Ministério da Agricultura, Brasil. Retrieved from http://www.agricultura.gov.br/vegetal/culturas/feijao September 05, 2012.

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Inheritance of anthracnose resistance in common bean genotypes P.I. 207262 and AB 136

Brazilian Journal of Genetics ◽

10.1590/s0100-84551997000100011 ◽

1997 ◽

Vol 20 (1) ◽

pp. 59-62 ◽

Cited By ~ 10

Author(s):

M.C. Gonçalves-Vidigal ◽

Antônio A. Cardoso ◽

Clibas Vieira ◽

Luiz S. Saraiva

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Resistance Genes ◽

Dominant Gene ◽

Colletotrichum Lindemuthianum ◽

Single Dominant Gene ◽

Anthracnose Resistance ◽

Dominant Genes

Bean (Phaseolus vulgaris) lines P.I. 207262 and AB 136, both resistant to delta and kappa races of Colletotrichum lindemuthianum, were crossed with Michelite, Dark Red Kidney, and Perry Marrow, susceptible to both races, and with Cornell 49-242, resistant to delta and susceptible to kappa. F1 and F2 reactions demonstrated that P.I. 207262 carries duplicate dominant genes for resistance to the delta race; AB 136 carries a dominant gene. These resistance genes are independent of the Are gene from Cornell 49-242. With respect to the kappa race, F1 and F2 data showed that the resistance controlled by P.I. 207262 and by AB 136 depends on a single dominant gene. Complementary factors were involved with AB 136 resistance to the delta race and with P.I. 207262 resistance to kappa.

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Inheritance of Resistance to Passionfruit Woodiness Virus in Common Bean (Phaseolus vulgaris L.)

HortScience ◽

10.21273/hortsci.35.5.880 ◽

2000 ◽

Vol 35 (5) ◽

pp. 880-881 ◽

Cited By ~ 2

Author(s):

R. Provvidenti

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Dominant Gene ◽

Bean Common Mosaic Virus ◽

Inheritance Of Resistance ◽

Single Dominant Gene ◽

Phaseolus Vulgaris L ◽

Rooted Cuttings ◽

Backcross Populations ◽

Dark Green

Passionfruit woodiness virus (PWV) can infect bean (Phaseolus vulgaris L.), causing a light and dark green foliar mosaic, veinbanding, downward curling, and plant stunting. The intensity of these symptoms can vary with the strain of the virus and cultivar, but they resemble those caused by bean common mosaic virus. In genetic populations derived from crosses and backcrosses involving cultivars that are resistant (`Black Turtle 1', `Clipper', and `RedKote') or susceptible (`Black Turtle 2', `California Light Red Kidney', and `Pioneer'), a single dominant gene conferred resistance to an Australian strain PWV-K. To this gene, the symbol Pwv (Passionfruit woodiness virus) is tentatively assigned. In plants derived from rooted cuttings of backcross populations, the same factor also conditioned resistance to three other Australian strains, PWV-Mild, PWV-51, and PWV-Tip Blight.

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A Single Dominant Gene Controlling Resistance to Soil Zinc Deficiency in Common Bean

Crop Science ◽

10.2135/cropsci2002.1071 ◽

2002 ◽

Vol 42 (4) ◽

pp. 1071-1074 ◽

Cited By ~ 20

Author(s):

Shree P. Singh ◽

Dale T. Westermann

Keyword(s):

Common Bean ◽

Zinc Deficiency ◽

Dominant Gene ◽

Single Dominant Gene ◽

Soil Zinc

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Inheritance of Resistance to Root-knot Nematodes (Meloidogyne sp.) in Prunus Rootstocks

HortScience ◽

10.21273/hortsci.35.7.1344 ◽

2000 ◽

Vol 35 (7) ◽

pp. 1344-1346 ◽

Cited By ~ 16

Author(s):

Zhen-Xiang Lu ◽

Gregory L. Reighard ◽

Andrew P. Nyczepir ◽

Thomas G. Beckman ◽

David W. Ramming

Keyword(s):

Meloidogyne Incognita ◽

Dominant Gene ◽

Signs And Symptoms ◽

Nematode Species ◽

Inheritance Of Resistance ◽

Stem Cuttings ◽

Root Knot Nematode ◽

Segregation Ratios ◽

Dominant Genes ◽

Meloidogyne Sp

Two F1 hybrid Prunus rootstocks, K62-68 and P101-41, developed from a cross of `Lovell' [susceptible to both Meloidogyne incognita (Kofoid and White) Chitwood and M. javanica (Treub) Chitwood] and `Nemared' (resistant to both root-knot nematode species), were selfed to produce two F2 seedling populations. Vegetative propagation by herbaceous stem cuttings was used to produce four or eight self-rooted plants of each F2 seedling for treatment replications. Eggs of M. incognita and M. javanica were inoculated into the potted media where plants were transplanted, and plants were harvested and roots examined for signs and symptoms associated with root-knot nematode infection ≈120 days later. Segregation ratios in both F2 families suggested that resistance to M. incognita in `Nemared' is controlled by two dominant genes (Mi and Mij) and that to M. javanica by a single dominant gene (Mij). Thus, Mij conveys resistance to both M. incognita and M. javanica.

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Occurrence of Meloidogyne enterolobii in common bean in southern Goiás State, Brazil

Ciência Rural ◽

10.1590/0103-8478cr20200403 ◽

2021 ◽

Vol 51 (10) ◽

Author(s):

Rodrigo Vieira da Silva ◽

José Orlando de Oliveira ◽

José Humberto de Ávila Júnior ◽

Brenda Ventura de Lima ◽

Nádia Fernandes Moreira

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Malate Dehydrogenase ◽

Root Systems ◽

Nematode Species ◽

Root Surface ◽

The State ◽

Root Knot Nematode ◽

Varying Thickness ◽

Meloidogyne Enterolobii

ABSTRACT: Meloidogyne enterolobii is known as the most aggressive root-knot nematode (RKN) in Brazil. Common bean (Phaseolus vulgaris) cultivated in the experimental field in Morrinhos, Southern Goiás State, exhibited symptoms typical to those caused by RKN. In order to clarify the etiology of the disease, root systems were analyzed. Typical galls of varying thickness were observed, with thickening larger than 3 cm in diameter containing RKN females inside roots and egg masses on the root surface. The identification of the nematode species was confirmed by isoenzyme electrophoresis (esterase and malate dehydrogenase phenotypes). This is the first report of M. enterolobii in Goiás and in Brazil parasitizing common bean under field conditions. This result is an alert to producers and to the State phytosanitary defense authorities, in order to prevent the further dissemination of this pest, especially in fields of common bean and industrial tomato in the State.

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Resistance to Lettuce Infectious Yellows Virus in Melon

HortScience ◽

10.21273/hortsci.33.3.534b ◽

1998 ◽

Vol 33 (3) ◽

pp. 534b-534

Author(s):

James D. McCreight

Keyword(s):

Cucumis Melo ◽

Dominant Gene ◽

Field Tests ◽

Single Dominant Gene ◽

Limited Data ◽

Sweetpotato Whitefly ◽

Sources Of Resistance ◽

Lettuce Infectious Yellows Virus ◽

Greenhouse Tests

Yellowing of melon (Cucumis melo L.) incited by lettuce infectious yellows virus (LIYV) reduces yield and fruit quality of infected plants. LIYV is transmitted only by the sweetpotato whitefly (Bemisia tabaci Genn.). Two naturally infected field tests indicated several potential sources of resistance to LIYV. PI 124112 and `Snake Melon' had mild symptoms in both field tests whereas PI 313970 was asymptomatic in the test in which it was included. In greenhouse tests using controlled inoculation, PI 313970 was asymptomatic, had negative ELISA assays for LIYV, and was negative for LIYV in serial transfers to Chenopodium. `Top Mark' and `PMR 5' were symptomatic, had positive ELISA assays for LIYV, and were positive for LIYV in serial transfers to Chenopodium in these greenhouse tests. Limited data indicate that resistance in PI 313970 is conditioned by a single, dominant gene.

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Histological Characterization of Resistance to Different Root-Knot Nematode Species Related to Phenolics Accumulation in Capsicum annuum

Phytopathology ◽

10.1094/phyto-95-0158 ◽

2005 ◽

Vol 95 (2) ◽

pp. 158-165 ◽

Cited By ~ 80

Author(s):

A. Pegard ◽

G. Brizzard ◽

A. Fazari ◽

O. Soucaze ◽

P. Abad ◽

...

Keyword(s):

Resistance Gene ◽

Capsicum Annuum ◽

High Performance ◽

Nematode Species ◽

Susceptible Cultivar ◽

Resistant Line ◽

Root Knot Nematode ◽

Resistance Response ◽

Root Knot Nematodes ◽

Chromatography Analysis

In the pepper Capsicum annuum CM334, which is used by breeders as a source of resistance to Phytophthora spp. and potyviruses, a resistance gene entirely suppresses reproduction of the root-knot nematode (Meloidogyne spp.). The current study compared the histological responses of this resistant line and a susceptible cultivar to infection with the three most damaging root-knot nematodes: M. arenaria, M. incognita, or M. javanica. Resistance of CM334 to root-knot nematodes was associated with unidentified factors that limited nematode penetration and with post-penetration biochemical responses, including the hypersensitive response, which apparently blocked nematode migration and thereby prevented juvenile development and reproduction. High-performance liquid chromatography analysis suggested that phenolic compounds, especially chlorogenic acid, may be involved in CM334 resistance. The response to infection in the resistant line varied with root-knot nematode species and was correlated with nematode behavior and pathogenicity in the susceptible cultivar: nematode species that quickly reached the vascular cylinder and initiated feeding sites in the susceptible cultivar were quickly recognized in CM334 and stopped in the epidermis or cortex. After comparing our data with those from other resistant pepper lines, we suggest that timing of the resistance response and the mechanism of resistance vary with plant genotype, resistance gene, and root-knot nematode species.

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