Protective Effect of
            Larportea aestuans
            Extract on Diclofenac ‐ Induced Oxidative Stress in the Brain of Male Wistar Rats

The aim of this study was to examine the effects of a methionine-enriched diet on anxiety-related behavior in rats and to determine the role of the brain oxidative status in these alterations. Adult male Wistar rats were fed from the 30th to 60th postnatal day with standard or methionine-enriched diet (double content comparing with standard diet: 7.7 g/kg). Rats were tested in open field and light–dark tests and afterwards oxidative status in the different brain regions were determined. Hyperhomocysteinemia induced by methionine-enriched diet in this study decreased the number of rearings, as well as the time that these animals spent in the center of the open field, but increased index of thigmotaxy. Oxidative status was selectively altered in the examined regions. Lipid peroxidation was significantly increased in the cortex and nc. caudatus of rats developing hyperhomocysteinemia, but unaltered in the hippocampus and thalamus. Based on the results of this research, it could be concluded that hyperhomocysteinemia induced by methionine nutritional overload increased anxiety-related behavior in rats. These proanxiogenic effects could be, at least in part, a consequence of oxidative stress in the rat brain.

Download Full-text

Differential oxidative stress and DNA damage in rat brain regions and blood following chronic arsenic exposure

Toxicology and Industrial Health ◽

10.1177/0748233708093355 ◽

2008 ◽

Vol 24 (4) ◽

pp. 247-256 ◽

Cited By ~ 42

Author(s):

D Mishra ◽

SJS Flora

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Drinking Water ◽

Wistar Rats ◽

Arsenic Exposure ◽

Brain Regions ◽

Single Strand ◽

Male Wistar Rats ◽

Chronic Arsenic Exposure ◽

The Brain

Chronic arsenic poisoning caused by contaminated drinking water is a wide spread and worldwide problem particularly in India and Bangladesh. One of the possible mechanisms suggested for arsenic toxicity is the generation of reactive oxygen species (ROS). The present study was planned 1) to evaluate if chronic exposure to arsenic leads to oxidative stress in blood and brain – parts of male Wistar rats and 2) to evaluate which brain region of the exposed animals was more sensitive to oxidative injury. Male Wistar rats were exposed to arsenic (50 ppm sodium arsenite in drinking water) for 10 months. The brain was dissected into five major parts, pons medulla, corpus striatum, cortex, hippocampus, and cerebellum. A number of biochemical variables indicative of oxidative stress were studied in blood and different brain regions. Single-strand DNA damage using comet assay was also assessed in lymphocytes. We observed a significant increase in blood and brain ROS levels accompanied by the depletion of GSH/GSSG ratio and glucose-6-phosphate dehydrogenase (G6PD) activity in different brain regions of arsenic-exposed rats. Chronic arsenic exposure also caused significant single-strand DNA damage in lymphocytes as depicted by comet with a tail in arsenic-exposed cells compared with the control cells. On the basis of results, we concluded that the cortex region of the brain was more sensitive to oxidative injury compared with the other regions studied. The present study, thus, leads us to suggest that arsenic induces differential oxidative stress in brain regions with cortex followed by hippocampus and causes single-strand DNA damage in lymphocytes.

Download Full-text

Riboceine Regimen Attenuates Ethanol-induced Neuronal Damage in the Cerebellum of Adult Male Wistar Rats

Iranian Jornal of Toxicology ◽

10.32598/ijt.15.4.778.1 ◽

2021 ◽

Vol 15 (4) ◽

pp. 249-256

Author(s):

Taiwo Adekemi Abayomi ◽

◽

Olorunfemi Samuel Tokunbo ◽

Moyinoluwa Ajayi ◽

Olawale Ayobami Abayomi ◽

...

Keyword(s):

Oxidative Stress ◽

Wistar Rats ◽

Neuronal Damage ◽

Motor Impairment ◽

Stress Profile ◽

Stress Markers ◽

Ethanol Toxicity ◽

Male Wistar Rats ◽

Cerebellar Astrocytes ◽

The Brain

Background: Although ethanol exerts its neurotoxic effect on the brain through inflammatory and oxidative processes, the effect of Riboceine on the brain following ethanol neurotoxicity is yet to be elucidated. Therefore, this study was designed to evaluate the effects of riboceine on the cellular, behavioral, and molecular impairments induced by ethanol toxicity in rats. Methods: A total of 24 male Wistar rats weighing between 160-170 grams were used for the study, and were divided into four groups of six rats each. After completion of the administration of ethanol and riboceine, and testing for motor impairment, the rats were sacrificed. The cerebellum was excised and processed for oxidative stress analyses, based on oxidative stress markers and histological examinations. The immunohistochemical expression of astrocytes in the cerebellum was examined, using Glial Fibrillary Acidic Protein (GFAP) stain. Results: This study demonstrated that ethanol-induced neurotoxicity in the cerebellum, characterized by increased oxidative stress profile, astrocyte activation, and neuronal death in the cerebellum, especially the Purkinje layer. Necrosis, significant decrease in Superoxide Dismutase (SOD), Catalase (CAT) and Gluathione (GSH) activities (P<0.05) as well as astrogliosis was associated with ethanol treatment. However, riboceine was observed to significantly increase the cerebellar SOD, CAT and GSH activities with significantly reduced Malondialdehyde (MDA) levels (P<0.05). It also attenuated the histomorphological alteration of the cerebellum and reduced the cerebellar astrocytes activation following ethanol-induced neurotoxicity, thus leading to the attenuation of motor impairment. Conclusion: Riboceine attenuated motor impairment caused by chronic ethanol-induced neurotoxicity, suggestive of its anti-oxidative and anti-inflammatory properties.

Download Full-text

Reversal effect of Solanum dasyphyllum against rotenone-induced neurotoxicity

Current Issues in Pharmacy and Medical Sciences ◽

10.2478/cipms-2020-0034 ◽

2020 ◽

Vol 33 (4) ◽

pp. 191-196

Author(s):

Omotayo B. Ilesanmi ◽

Obade Efe ◽

Temitope T. Odewale ◽

Frances O. Atanu ◽

Esther F. Adeogun ◽

...

Keyword(s):

Oxidative Stress ◽

Protective Effect ◽

In Vivo Model ◽

Respiratory Enzymes ◽

Reversal Effect ◽

Male Wistar Rats ◽

Markers Of Oxidative Stress ◽

The Brain ◽

Rotenone Exposure

Abstract We earlier reported the protective effect of Solanum dasyphyllum against cyanide neurotoxicity. In furtherance to this, we investigated the protective effect of S. dasyphyllum against rotenone, a chemical toxin that causes brain-related diseases. Mitochondria fraction obtained from the brain of male Wistar rats was incubated with various solvents (hexane, dichloromethane, ethylacetate, and methanol) extracts of S. dasyphyllum before rotenone exposure. Mitochondria respiratory enzymes (MRE) were evaluated along with markers of oxidative stress. The inhibition of MRE by rotenone was reversed by treatment with various fractions of S. dasyphyllum. The oxidative stress induced by rotenone was also reversed by fractions of S. dasyphyllum. In addition, the ethylacetate fraction of S. dasyphyllum was most potent against rotenone-induced neurotoxicity. In conclusion, S. dasyphyllum is rich in active phytochemicals that can prevent some neurotoxic effects of rotenone exposure. Further study can be done in an in vivo model to substantiate our results.

Download Full-text

Faculty Opinions recommendation of Protective effect of apigenin on ethylene glycol-induced urolithiasis via attenuating oxidative stress and inflammatory parameters in adult male Wistar rats.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.740191309.793586242 ◽

2021 ◽

Author(s):

Walter Strohmaier

Keyword(s):

Oxidative Stress ◽

Ethylene Glycol ◽

Protective Effect ◽

Adult Male ◽

Wistar Rats ◽

Inflammatory Parameters ◽

Male Wistar Rats

Download Full-text

Therapeutic effect of methanolic extract of Laportea aestuans (L.) Chew, on oxidative stress in the brain of male Wistar rats

10.1063/1.5033408 ◽

2018 ◽

Author(s):

Omotosho Omolola Elizabeth ◽

Ogunlade Oladipupo Olawumi

Keyword(s):

Oxidative Stress ◽

Therapeutic Effect ◽

Wistar Rats ◽

Methanolic Extract ◽

Male Wistar Rats ◽

The Brain

Download Full-text

Protective effect of Moringa oleifera oil against HgCl2-induced hepato- and nephro-toxicity in rats

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2016-0033 ◽

2017 ◽

Vol 28 (4) ◽

Cited By ~ 10

Author(s):

Sunny O. Abarikwu ◽

Sussan Benjamin ◽

Sunday Godspower Ebah ◽

Godbless Obilor ◽

Goodluck Agbam

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Protective Effect ◽

Wistar Rats ◽

Moringa Oleifera ◽

Negative Effects ◽

Male Wistar Rats ◽

Moringa Oleifera Oil

Abstract:Background:Various parts of theMethods:Male Wistar rats were administered MO (1.798 mg/kg p.o.) or HgClResults:In the liver, malondialdehyde (MDA) level, glutathione (GSH), and superoxide dismutase (SOD) activities were higher whereas catalase (CAT) activity was lower in the HgClConclusions:MO decreased the negative effects of Hg-induced oxidative stress in rats.

Download Full-text