Objective: This investigation involves the extraction, isolation, and characterization of chemical constituents from a Barringtonia family plant, Barringtonia asiatica followed by Antibacterial, cytotoxicity and evaluation of its antioxidant principles. Methods: The dried stem-bark powders were subjected to sequential soaking with polar and nonpolar solvents and extraction using rota-vap. Dichloromethane extract reveals the presence of significant amount of phytochemicals. The dichloromethane extract was subjected to isolation using column chromatographic analysis with solvents such as, dichloromethane, chloroform, hexane, ethyl acetate and methanol. Further, the isolated compound was subjected to thin layer chromatography technique and spectral analysis such as infrared, 1H-NMR, 13C-NMR, FT-IR, and mass spectroscopy. The antibacterial using agar disc method. Results: The compound was isolated in hexane: ethyl acetate (9:1) and dichloromethane: ethyl acetate in the solvent system in the ratio of 9:1 and 7:3, respectively using column chromatographic technique. On the basis of phytochemical, chromatographic, and spectral analysis, the isolated compounds were identified as Nerolidiol and Heneicosane. Conclusion: This compound was isolated for the first time from the stem-bark of Barringtonia asiatica. The in vitro antioxidant assay of isolated compounds has shown a dose-dependent increase in free radical scavenging activity using DPPH, the antibacterial and artemia salina cytotoxicity testing showed a significant result. The chromatographic separation led to the isolation of Nerolidiol (1) and Heneicosane (2). Their structures were determined by 1H-NMR, 13CNMR, IR and MS data analysis as well as by comparison of their data with the published values.
Detection and purification of lectins and glycoproteins by a non‐column chromatographic technique
