Sphingosine 1‐phosphate regulates innate and acquired intestinal IgA production

AbstractPrevious studies have shown that a 10 % cocoa (C10) diet, containing polyphenols and fibre among others, modifies intestinal and systemic Ig production. The present study aimed at evaluating the impact of C10 on IgA and IgM production in the intestinal and extra-intestinal mucosal compartments, establishing the involvement of cocoa fibre (CF) in such effects. Mechanisms by which C10 intake may affect IgA synthesis in the salivary glands were also studied. To this effect, rats were fed either a standard diet, a diet containing C10, CF or inulin. Intestinal (the gut wash (GW), Peyer’s patches (PP) and mesenteric lymph nodes (MLN)) and extra-intestinal (salivary glands) mucosal tissues and blood samples were collected for IgA and IgM quantification. The gene expressions of IgA production- and homing-related molecules were studied in the salivary glands. The C10 diet decreased intestinal IgA and IgM production. Although the CF diet decreased the GW IgA concentration, it increased PP, MLN and serum IgA concentrations. Both the C10 and the CF diets produced a down-regulatory effect on IgA secretion in the extra-intestinal tissues. The C10 diet interacted with the mechanisms involved in IgA synthesis, whereas the CF showed particular effects on the homing and transcytosis of IgA across the salivary glands. Overall, CF was able to up-regulate IgA production in the intestinal-inductor compartments, whereas it down-regulated its production at the mucosal-effector ones. Further studies must be directed to ascertain the mechanisms involved in the effect of particular cocoa components on gut-associated lymphoid tissue.

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Low Intestinal IGA Production in CVID Facilitates Pathobiont-Mediated IFN Responses and Enteropathy

Gastroenterology ◽

10.1016/s0016-5085(17)33381-4 ◽

2017 ◽

Vol 152 (5) ◽

pp. S997-S998 ◽

Cited By ~ 1

Author(s):

Natalia shulzhenko ◽

Xiaoxi Dong ◽

Dariia Vyshenska ◽

Renee Greer ◽

Manoj Gurung ◽

...

Keyword(s):

Iga Production ◽

Intestinal Iga

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Sphingosine 1-phosphate regulates peritoneal B-cell trafficking for subsequent intestinal IgA production

Blood ◽

10.1182/blood-2006-08-041582 ◽

2007 ◽

Vol 109 (9) ◽

pp. 3749-3756 ◽

Cited By ~ 67

Author(s):

Jun Kunisawa ◽

Yosuke Kurashima ◽

Masashi Gohda ◽

Morio Higuchi ◽

Izumi Ishikawa ◽

...

Keyword(s):

B Cells ◽

Lymph Nodes ◽

B Cell ◽

Antibody Production ◽

Pivotal Role ◽

Secretory Iga ◽

Lymphoid Tissues ◽

Sphingosine 1 Phosphate ◽

S1p Receptor ◽

Iga Production

AbstractSphingosine 1-phosphate (S1P) is known to play a pivotal role in the regulation of lymphocyte emigration from organized lymphoid tissues such as the peripheral lymph nodes and thymus, but its immunologic role in unorganized and diffused tissues remains to be elucidated. Here we show that the trafficking of peritoneal B cells is principally regulated by S1P. All peritoneal B cells including B1a, B1b, and B2 B cells express comparable levels of the type 1 S1P receptor. Thus, treatment with FTY720, an S1P receptor modulator, caused the rapid disappearance of peritoneal B cells by inhibiting both their emigration from parathymic lymph nodes and their recirculation from the blood into the peritoneal cavity without affecting their progenitor populations. These changes did not affect natural plasma antibody production or phosphorylcholine (PC)–specific antibody production in serum after peritoneal immunization with heat-killed Streptococcal pneumoniae (R36A). However, FTY720 dramatically reduced peritoneal B cell-derived natural intestinal secretory IgA production without affecting the expression of J-chain and polyimmunoglobulin receptors. Additionally, FTY720 impaired the generation of PC-specific fecal IgA responses after oral immunization with R36A. These findings point to a pivotal role for S1P in connecting peritoneal B cells with intestinal B-cell immunity.

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Sphingosine 1-Phosphate Regulates the Egress of IgA Plasmablasts from Peyer’s Patches for Intestinal IgA Responses

The Journal of Immunology ◽

10.4049/jimmunol.180.8.5335 ◽

2008 ◽

Vol 180 (8) ◽

pp. 5335-5343 ◽

Cited By ~ 60

Author(s):

Masashi Gohda ◽

Jun Kunisawa ◽

Fumi Miura ◽

Yuki Kagiyama ◽

Yosuke Kurashima ◽

...

Keyword(s):

Peyer's Patches ◽

Peyer’S Patches ◽

Sphingosine 1 Phosphate ◽

Intestinal Iga

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IgA dysfunctions induced by the early-lifetime disruption of gut microbitoa result in metabolic syndrome in mice

10.21203/rs.3.rs-40336/v1 ◽

2020 ◽

Author(s):

Jielong Guo ◽

Xue Han ◽

Yilin You ◽

Weidong Huang ◽

Zhan Jicheng

Keyword(s):

Metabolic Syndrome ◽

Low Dose ◽

Newborn Mice ◽

Germ Free ◽

Small Intestinal ◽

Iga Production ◽

Induced Changes ◽

Intestinal Iga

Abstract Backgroud: Disruption of the gut microbiota (GM), mainly induced by antibiotic treatments and C-sections, is prevalent during the early lifetime, which can result in lifelong changes in the GM composition and metabolism.Results: The GM of newborn mice was influenced after being subjected to transitory treatment with low-dose penicillin (LDP), resulting in a permanent reduction of intestinal IgA. Germ-free (GF) mice transferred GM from the LDP-treated mice also showed decreased intestinal IgA levels. Similarly, antigens derived from the LDP-treated mice induced lower IgA production during in vitro incubation with small intestinal tissues. Furthermore, a lack of intestinal IgA led to the persistent dysbiosis of mucosal GM, causing metabolic syndrome (MetS) in the LDP-treated mice. The mice lacking intestinal IgA (Pigr-/-) only showed transient alteration in GM after LDP exposure while the long-period metabolism was not influenced. Moreover, gavage with GM from the LDP-free mice or probiotics (partially) restored the GM and intestinal IgA, while improving the MetS in LDP-treated mice.Conclusions: The antibiotics–induced changes of GM in early lifetime permanently dampened the IgA responses to the GM, which lead to the long-term dysbiosis of intestinal mucosal bacteria and MetS.

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