scholarly journals Insulin‐stimulated neuronal nitric oxide synthase phosphorylation is AKT2‐dependent in C2C12 skeletal muscle cells (1013.13)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Kathryn Hinchee‐Rodriguez ◽  
Martin Adamo ◽  
Bettie Sue Masters ◽  
Linda Roman
Keyword(s):  
Nitric Oxide ◽  
Skeletal Muscle ◽  
Nitric Oxide Synthase ◽  
Neuronal Nitric Oxide Synthase ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
C2c12 Skeletal Muscle Cells ◽  
Oxide Synthase

Regulation of inducible nitric oxide synthase expression in L6 rat skeletal muscle cells

1997 ◽  
Vol 272 (1) ◽  
pp. C35-C40 ◽  
Author(s):  
S. Okuda ◽  
F. Kanda ◽  
Y. Kawahara ◽  
K. Chihara
Keyword(s):  
Nitric Oxide ◽  
Skeletal Muscle ◽  
Protein Kinase ◽  
Nitric Oxide Synthase ◽  
Tyrosine Kinase ◽  
Muscle Cells ◽  
Inos Expression ◽  
Skeletal Muscle Cells ◽  
Rat Skeletal Muscle ◽  
Oxide Synthase

Cytokine-stimulated expression of inducible type of nitric oxide synthase (iNOS) seems to be regulated by various signal pathways in a cell-specific manner. In this study, we examined how it was regulated in L6 rat skeletal muscle cells. In L6 cells, the combination of interleukin-1 beta and interferon-gamma induced a marked accumulation of nitrite, a stable metabolite of nitric oxide. In parallel with this reaction, iNOS mRNA expression was achieved at a maximum between 3 and 6 h, and iNOS protein was detectable at 6 h and peaked at 24 h after stimulation. Tyrosine kinase inhibitors, herbimycin A, and genistein suppressed cytokine-induced iNOS expression and nitrite production. Forskolin, an adenosine 3',5'-cyclic monophosphate-dependent protein kinase (PKA) activator, and phorbol 12-myristate 13-acetate, a protein kinase C (PKC)-activating phorbol ester, enhanced these cytokine-induced reactions. These results indicate that iNOS expression by cytokines is mediated via a protein tyrosine kinase-dependent pathway and is positively modulated by both PKA- and PKC-dependent pathways in this cell type.

scholarly journals Skeletal muscle glucose uptake during treadmill exercise in neuronal nitric oxide synthase-μ knockout mice

2016 ◽  
Vol 310 (10) ◽  
pp. E838-E845 ◽  
Author(s):  
Yet Hoi Hong ◽  
Christine Yang ◽  
Andrew C. Betik ◽  
Robert S. Lee-Young ◽  
Glenn K. McConell
Keyword(s):  
Nitric Oxide ◽  
Skeletal Muscle ◽  
Nitric Oxide Synthase ◽  
Glucose Uptake ◽  
Neuronal Nitric Oxide Synthase ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Skeletal Muscle Contraction ◽  
Skeletal Muscle Glucose Uptake ◽  
Oxide Synthase

Nitric oxide influences intramuscular signaling that affects skeletal muscle glucose uptake during exercise. The role of the main NO-producing enzyme isoform activated during skeletal muscle contraction, neuronal nitric oxide synthase-μ (nNOSμ), in modulating glucose uptake has not been investigated in a physiological exercise model. In this study, conscious and unrestrained chronically catheterized nNOSμ+/+ and nNOSμ−/− mice either remained at rest or ran on a treadmill at 17 m/min for 30 min. Both groups of mice demonstrated similar exercise capacity during a maximal exercise test to exhaustion (17.7 ± 0.6 vs. 15.9 ± 0.9 min for nNOSμ+/+ and nNOSμ−/−, respectively, P > 0.05). Resting and exercise blood glucose levels were comparable between the genotypes. Very low levels of NOS activity were detected in skeletal muscle from nNOSμ−/− mice, and exercise increased NOS activity only in nNOSμ+/+ mice (4.4 ± 0.3 to 5.2 ± 0.4 pmol·mg−1·min−1, P < 0.05). Exercise significantly increased glucose uptake in gastrocnemius muscle (5- to 7-fold) and, surprisingly, more so in nNOSμ−/− than in nNOSμ+/+ mice ( P < 0.05). This is in parallel with a greater increase in AMPK phosphorylation during exercise in nNOSμ−/− mice. In conclusion, nNOSμ is not essential for skeletal muscle glucose uptake during exercise, and the higher skeletal muscle glucose uptake during exercise in nNOSμ−/− mice may be due to compensatory increases in AMPK activation.

Role of Neuronal Nitric Oxide Synthase in Modulating Microvascular and Contractile Function in Rat Skeletal Muscle

2011 ◽  
Vol 18 (6) ◽  
pp. 501-511 ◽  
Author(s):  
STEVEN W. COPP ◽  
DANIEL M. HIRAI ◽  
SCOTT K. FERGUSON ◽  
TIMOTHY I. MUSCH ◽  
DAVID C. POOLE
Keyword(s):  
Nitric Oxide ◽  
Skeletal Muscle ◽  
Nitric Oxide Synthase ◽  
Contractile Function ◽  
Neuronal Nitric Oxide Synthase ◽  
Rat Skeletal Muscle ◽  
Oxide Synthase

scholarly journals Neuronal nitric oxide synthase mediates insulin- and oxidative stress-induced glucose uptake in skeletal muscle myotubes

2017 ◽  
Vol 110 ◽  
pp. 261-269 ◽  
Author(s):  
Dean L. Kellogg ◽  
Karen M. McCammon ◽  
Kathryn S. Hinchee-Rodriguez ◽  
Martin L. Adamo ◽  
Linda J. Roman
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Skeletal Muscle ◽  
Nitric Oxide Synthase ◽  
Glucose Uptake ◽  
Neuronal Nitric Oxide Synthase ◽  
Oxide Synthase ◽  
And Oxidative Stress

scholarly journals Up-regulation of the peroxiredoxin-6 related metabolism of reactive oxygen species in skeletal muscle of mice lacking neuronal nitric oxide synthase

2009 ◽  
Vol 587 (3) ◽  
pp. 655-668 ◽  
Author(s):  
Luis Da Silva-Azevedo ◽  
Sebastian Jähne ◽  
Christian Hoffmann ◽  
Daniel Stalder ◽  
Manfred Heller ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Skeletal Muscle ◽  
Nitric Oxide Synthase ◽  
Reactive Oxygen ◽  
Neuronal Nitric Oxide Synthase ◽  
Oxygen Species ◽  
Peroxiredoxin 6 ◽  
Oxide Synthase
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