Effects of plant stanol esters on LDL receptor protein expression and on LDL receptor and HMG‐CoA reductase mRNA expression in mononuclear blood cells of healthy men and women

2001 ◽  
Vol 16 (2) ◽  
pp. 1-16 ◽  
Author(s):  
Jogchum Plat ◽  
Ronald P. Mensink
Keyword(s):  
Blood Cells ◽  
Ldl Receptor ◽  
Receptor Protein ◽  
Men And Women ◽  
Healthy Men ◽  
Plant Stanol ◽  
Reductase Mrna ◽  
Coa Reductase ◽  
Plant Stanol Esters ◽  
Mononuclear Blood Cells

Effects of ezetimibe and/or simvastatin on LDL receptor protein expression and on LDL receptor and HMG-CoA reductase gene expression: A randomized trial in healthy men

2008 ◽  
Vol 198 (1) ◽  
pp. 198-207 ◽  
Author(s):  
Ioanna Gouni-Berthold ◽  
Heiner K. Berthold ◽  
Helena Gylling ◽  
Maarit Hallikainen ◽  
Eleni Giannakidou ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Expression ◽  
Randomized Trial ◽  
Ldl Receptor ◽  
Receptor Protein ◽  
Hmg Coa Reductase ◽  
Healthy Men ◽  
Reductase Gene ◽  
Coa Reductase

scholarly journals Adzuki resistant starch lowered serum cholesterol and hepatic 3-hydroxy-3-methylglutaryl-CoA mRNA levels and increased hepatic LDL-receptor and cholesterol 7α-hydroxylase mRNA levels in rats fed a cholesterol diet

2005 ◽  
Vol 94 (6) ◽  
pp. 902-908 ◽  
Author(s):  
Kyu-Ho Han ◽  
Miharu Iijuka ◽  
Ken-ichiro Shimada ◽  
Mitsuo Sekikawa ◽  
Katsuhisa Kuramochi ◽  
...  
Keyword(s):  
Bile Acid ◽  
Serum Cholesterol ◽  
Resistant Starch ◽  
Mrna Level ◽  
Ldl Receptor ◽  
Mrna Levels ◽  
Cholesterol Diet ◽  
Receptor Mrna ◽  
Reductase Mrna ◽  
Coa Reductase

We examined the effects of adzuki bean resistant starch on serum cholesterol and hepatic mRNA in rats fed a cholesterol diet. The mRNA coded for key regulatory proteins of cholesterol metabolism. The control rats were fed 15 % cornstarch (basal diet, BD). The experimental rats were fed BD plus a 0·5 % cholesterol diet (CD), or a 15 % adzuki resistant starch plus 0·5 % cholesterol diet (ACD) for 4 weeks. The serum total cholesterol and VLDL + intermediate density lipoprotein + LDL-cholesterol levels in the ACD group were significantly lower than those in the CD group throughout the feeding period. The total hepatic cholesterol concentrations in the CD and ACD groups were not significantly different. The faecal total bile acid concentration in the ACD group was significantly higher than that in the BD and CD groups. Total SCFA and acetic acid concentrations in the ACD group were significantly higher than those in the CD group but there were no significant differences in the concentrations between the ACD and BD groups. The hepatic LDL-receptor mRNA and cholesterol 7α-hydroxylase mRNA levels in the ACD group were significantly higher than those in the CD group and the hepatic 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase mRNA level in the ACD group was significantly lower than in the CD group. The results suggest that adzuki resistant starch has a serum cholesterol-lowering function via enhancement of the hepatic LDL-receptor mRNA and cholesterol 7α-hydroxylase mRNA levels and faecal bile acid excretion, and a decrease in the hepatic HMG-CoA reductase mRNA level, when it is added to a cholesterol diet.

scholarly journals Identification of liver CYP51 as a gene responsive to circulating cholesterol in a hamster model

2016 ◽  
Vol 5 ◽  
Author(s):  
Haiqiu Huang ◽  
Zhuohong Xie ◽  
Wallace Yokoyama ◽  
Liangli Yu ◽  
Thomas T. Y. Wang
Keyword(s):  
High Fat Diet ◽  
Cholesterol Metabolism ◽  
Ldl Receptor ◽  
High Fat ◽  
Hamster Model ◽  
Hmg Coa Reductase ◽  
Cholesterol Levels ◽  
Reductase Mrna ◽  
Coa Reductase ◽  
The Relationship

AbstractHypercholesterolaemia is a risk factor for CVD, which is a leading cause of death in industrialised societies. The biosynthetic pathways for cholesterol metabolism are well understood; however, the regulation of circulating cholesterol by diet is still not fully elucidated. The present study aimed to gain more comprehensive understanding of the relationship between circulating cholesterol levels and molecular effects in target tissues using the hamster model. Male golden Syrian hamsters were fed with chow or diets containing 36 % energy from fat with or without 1 % cholesteyramine (CA) as a modulator of circulating cholesterol levels for 35 d. It was revealed that the expression of lanosterol 14α-demethylase (CYP51) instead of 3-hydroxy-3-methyl-glutaryl (HMG)-CoA reductase mRNA expression was responsive to circulating cholesterol in hamsters fed hypercholesterolaemic diets. The high-fat diet increased circulating cholesterol and down-regulated CYP51, but not HMG-CoA reductase. The CA diet decreased cholesterol and increased CYP51 expression, but HMG-CoA reductase expression was not affected. The high-fat diet and CA diet altered the expression level of cholesterol, bile acids and lipid metabolism-associated genes (LDL receptor, cholesterol 7α-hydroxylase (CYP7A1), liver X receptor (LXR) α, and ATP-binding cassette subfamily G member 5/8 (ABCG5/8)) in the liver, which were significantly correlated with circulating cholesterol levels. Correlation analysis also showed that circulating cholesterol levels were regulated by LXR/retinoid X receptor and PPAR pathways in the liver. Using the hamster model, the present study provided additional molecular insights into the influence of circulating cholesterol on hepatic cholesterol metabolism pathways during hypercholesterolaemia.

scholarly journals Circadian rhythm in hepatic low-density-lipoprotein (LDL)-receptor expression and plasma LDL levels

1994 ◽  
Vol 298 (1) ◽  
pp. 39-43 ◽  
Author(s):  
S Balasubramaniam ◽  
A Szanto ◽  
P D Roach
Keyword(s):  
Low Density Lipoprotein ◽  
Circadian Variation ◽  
Ldl Receptor ◽  
Density Lipoprotein ◽  
Receptor Expression ◽  
Receptor Protein ◽  
Low Density ◽  
Hmg Coa Reductase ◽  
Cultured Fibroblasts ◽  
Coa Reductase

On the basis of studies in vivo and in vitro that involved the use of pharmacological amounts of drugs and hormones or excess cholesterol supplementation, the expression of the low-density lipoprotein (LDL) receptor appears to be tightly coupled to the regulation of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase activity and to extracellular levels of LDL. The present study was undertaken to see how these three entities are regulated under normal physiological conditions over a 24 h period. The results show that, in the rat, hepatic LDL-receptor expression and plasma LDL levels exhibit diurnal periodicity, with a 2-3-fold difference between the peak and trough of each rhythm. Both rhythms showed high inverse correlation (r = -0.86, P < 0.01), plasma LDL levels being lowest at the onset of darkness when LDL-receptor expression was at its peak. The results also showed that the LDL-receptor protein in rat liver has a shorter half-life than that reported for cultured fibroblasts or HepG2 cells. The maximal expression of the LDL receptor occurred several hours before the peak activity of HMG-CoA reductase and appeared not to be influenced by cellular or membrane cholesterol levels during the 24 h cycle. Treatment with dexamethasone increased the LDL-receptor activity significantly at both the lowest and highest points of the rhythm, but the receptor rhythm was still maintained, suggesting that the signal for the circadian variation of the receptor expression is not mediated by adrenal hormones.

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