scholarly journals Profilin2a‐phosphorylation as a regulatory mechanism for actin dynamics

2019 ◽  
Vol 34 (2) ◽  
pp. 2147-2160 ◽  
Author(s):  
Lisa Marie Walter ◽  
Peter Franz ◽  
Robert Lindner ◽  
Georgios Tsiavaliaris ◽  
Niko Hensel ◽  
...  
Keyword(s):  
Regulatory Mechanism ◽  
Actin Dynamics

scholarly journals Sequential phosphorylation of NDEL1 by the DYRK2-GSK3β complex is critical for neuronal morphogenesis

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Youngsik Woo ◽  
Soo Jeong Kim ◽  
Bo Kyoung Suh ◽  
Yongdo Kwak ◽  
Hyun-Jin Jung ◽  
...  
Keyword(s):  
Regulatory Mechanism ◽  
Actin Dynamics ◽  
Neural Connectivity ◽  
Filamentous Actin ◽  
Proteomics Analysis ◽  
Neuronal Morphogenesis ◽  
Regulatory Pathways ◽  
Human Kinome ◽  
Dendritic Structures ◽  
Brain Lysate

Neuronal morphogenesis requires multiple regulatory pathways to appropriately determine axonal and dendritic structures, thereby to enable the functional neural connectivity. Yet, however, the precise mechanisms and components that regulate neuronal morphogenesis are still largely unknown. Here, we newly identified the sequential phosphorylation of NDEL1 critical for neuronal morphogenesis through the human kinome screening and phospho-proteomics analysis of NDEL1 from mouse brain lysate. DYRK2 phosphorylates NDEL1 S336 to prime the phosphorylation of NDEL1 S332 by GSK3β. TARA, an interaction partner of NDEL1, scaffolds DYRK2 and GSK3β to form a tripartite complex and enhances NDEL1 S336/S332 phosphorylation. This dual phosphorylation increases the filamentous actin dynamics. Ultimately, the phosphorylation enhances both axonal and dendritic outgrowth and promotes their arborization. Together, our findings suggest the NDEL1 phosphorylation at S336/S332 by the TARA-DYRK2-GSK3β complex as a novel regulatory mechanism underlying neuronal morphogenesis.

scholarly journals Complex structures of Rsu1 and PINCH1 reveal a regulatory mechanism of the ILK/PINCH/Parvin complex for F-actin dynamics

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Haibin Yang ◽  
Leishu Lin ◽  
Kang Sun ◽  
Ting Zhang ◽  
Wan Chen ◽  
...  
Keyword(s):  
Actin Filaments ◽  
Regulatory Mechanism ◽  
Stress Fiber ◽  
Fiber Formation ◽  
Actin Dynamics ◽  
Complex Structures ◽  
Stress Fiber Formation ◽  
Cell Adhesion And Migration ◽  
And Migration ◽  
Leucine Rich Repeats

Communications between actin filaments and integrin-mediated focal adhesion (FA) are crucial for cell adhesion and migration. As a core platform to organize FA proteins, the tripartite ILK/PINCH/Parvin (IPP) complex interacts with actin filaments to regulate the cytoskeleton-FA crosstalk. Rsu1, a Ras suppressor, is enriched in FA through PINCH1 and plays important roles in regulating F-actin structures. Here, we solved crystal structures of the Rsu1/PINCH1 complex, in which the leucine-rich-repeats of Rsu1 form a solenoid structure to tightly associate with the C-terminal region of PINCH1. Further structural analysis uncovered that the interaction between Rsu1 and PINCH1 blocks the IPP-mediated F-actin bundling by disrupting the binding of PINCH1 to actin. Consistently, overexpressing Rsu1 in HeLa cells impairs stress fiber formation and cell spreading. Together, our findings demonstrated that Rsu1 is critical for tuning the communication between F-actin and FA by interacting with the IPP complex and negatively modulating the F-actin bundling.

The Variant rs145204276 of GAS5 is Associated with the Development and Prognosis of Gastric Cancer

2018 ◽  
Vol 27 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Qianjun Li ◽  
Gang Ma ◽  
Huimin Guo ◽  
Suhua Sun ◽  
Ying Xu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Survival Rate ◽  
Cancer Progression ◽  
Regulatory Mechanism ◽  
Tumor Stage ◽  
Kaplan Meier ◽  
Non Coding Rna ◽  
Early Tumor ◽  
Long Non Coding Rna ◽  
Clinicopathological Variables

Background & Aims: Down-regulation of the growth arrest specific transcript 5 (GAS5) (long non-coding RNA) is associated with cell proliferation of gastric cancer (GC) and a poor prognosis. We aimed to investigate whether the variant rs145204276 of GAS5 is associated with the prognosis of GC in the Chinese population, and to unveil the regulatory mechanism underlying the GAS5 expression in GC tissues.Method: 1,253 GC patients and 1,354 healthy controls were included. The frequency of the genotype del/del and the allele del of rs145204276 were compared between the patients and the controls and between different subgroups of patients classified by clinicopathological variables. The overall survival rate was analyzed according to the Kaplan-Meier method using the log-rank test.Results: The frequency of genotype del/del was significantly lower in patients than in the controls (7.0% vs. 9.1%, p = 0.001). Kaplan-Meier analysis showed that genotype del/del was significantly associated with a higher survival rate (p = 0.01). Patients with late tumor stage were found to have a significantly lower rate of genotype del/del than those with an early tumor stage (4.9% vs. 8.8%, p = 0.01). Patients with UICC III and IV were found to have a significantly lower rate of genotype del/del than those with UICC I and II (5.3% vs. 8.1%, p = 0.02).Conclusion: The variant rs145204276 of GAS5 is associated with the development and prognosis of GC. The allele del of rs145204276 is associated with a remarkably lower incidence of cancer progression and metastasis.

2195-P: Identification of the Regulatory Mechanism of mTORC1 Signaling Activity in Pancreatic Beta Cells in GCN2 Knockout Mice

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 2195-P
Author(s):  
MICHIYO KUDO ◽  
SHUN-ICHIRO ASAHARA ◽  
AYUMI KANNO ◽  
YOSHIAKI KIDO
Keyword(s):  
Beta Cells ◽  
Knockout Mice ◽  
Pancreatic Beta Cells ◽  
Regulatory Mechanism ◽  
Mtorc1 Signaling ◽  
Signaling Activity

Organic Consumer Behavior- Special reference to cereal /cereal products in the southern part of Tamilnadu.

Think India ◽  
2019 ◽  
Vol 22 (3) ◽  
pp. 757-767
Author(s):  
J. ELANCHEZHIAN ◽  
Dr. K. KALAICHELVI
Keyword(s):  
Organic Agriculture ◽  
Regulatory Mechanism ◽  
Organic Food ◽  
State Governments ◽  
Consumer Awareness ◽  
Cereal Products ◽  
Food Standards ◽  
Organic Products ◽  
The Usa ◽  
The Government

Consumers’ interest in organic products is increasing globally. As IFOAM 2016 report, only 1.2 % of the land has been utilized in organic agriculture method. The overall organic market has achieved 89.7 billion $ in 2016 in that, & 48.4 a billion in sales accounted for the USA and German alone. Total registered organic producer in the worldwide is 2.7 million in that India is the leading country which has 835,200 organic producers. But many of them are a small farmer, and they had shared 1.49 million hectares only. The Government of India (GOI) and the state governments have taken several steps to improve the regulatory mechanism and frame several schemes to incentivize organic farming. 2017 December, Food Standards and Safety Authority of India (FSSAI) have recognized both the certification systems (NPOP and PGS-India) valid for organic food products. From these steps, GOI has tried to create confidence in the organic products, so that, domestic consumers and export countries can trust Indian organic products. But still, the organic sector in India suffered from some unique characteristic that is the absence of proper branding, package, consumer awareness, purchasing power, and supply chain issues (Agarwal, 2018).

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