COMPARISON OF CROSS-LINKED HEMOGLOBIN (CLII) SOLUTION TO LACTATED RINGERS AND ALBUMIN SOLUTION IN RESUSCITATION OF A RAT MODEL OF ESCHERICHIA COLI (E. coli) SEPTIC SHOCK

ABSTRACT Invasive infections due to extended-spectrum-β-lactamase- and pAmpC-producing Escherichia coli (ESBL/pAmpC-EC) are an important cause of morbidity, often caused by the high-risk clone sequence type (ST131) and isolates classified as extraintestinal pathogenic E. coli (ExPEC). The relative influence of host immunocompetence versus microbiological virulence factors in the acquisition and outcome of bloodstream infections (BSI) is poorly understood. Herein, we used whole-genome sequencing on 278 blood culture isolates of ESBL/pAmpC-EC from 260 patients with community-onset BSI collected from 2012 to 2015 in Stockholm to study the association of virulence genes, sequence types, and antimicrobial resistance with severity of disease, infection source, ESBL/pAmpC-EC BSI low-risk patients, and patients with repeated episodes. ST131 subclade C2 comprised 29% of all patients. Factors associated with septic shock in multivariable analysis were patient host factors (hematologic cancer or transplantation and reduced daily living activity), presence of the E. coli virulence factor iss (increased serum survival), absence of phenotypic multidrug resistance, and absence of the genes pap and hsp. Adhesins, particularly pap, were associated with urinary tract infection (UTI) source, while isolates from post-prostate biopsy sepsis had a low overall number of virulence operons, including adhesins, and commonly belonged to ST131 clades A, B, and subclade C1, ST1193, and ST648. ST131 was associated with recurrent episodes. In conclusion, the most interesting finding is the association of iss with septic shock. Adhesins are important for UTI pathogenesis, while otherwise low-pathogenic isolates from the microbiota can cause post-prostate biopsy sepsis.

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A Single, Ultra‐High Dose Aminoglycoside Therapy in a Rat Model of E. coli Induced Septic Shock

The FASEB Journal ◽

10.1096/fasebj.27.1_supplement.lb546 ◽

2013 ◽

Vol 27 (S1) ◽

Author(s):

Amarnath Pisipati ◽

Kumar Anand ◽

Robert Ariano ◽

Brian Blakley ◽

Ehsan K Esfahani

Keyword(s):

Septic Shock ◽

Rat Model ◽

High Dose ◽

E Coli ◽

Aminoglycoside Therapy

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A Uropathogenicity Island Contributes to the Pathogenicity of Escherichia coli Strains That Cause Neonatal Meningitis

Infection and Immunity ◽

10.1128/iai.70.10.5865-5869.2002 ◽

2002 ◽

Vol 70 (10) ◽

pp. 5865-5869 ◽

Cited By ~ 32

Author(s):

Véronique Houdouin ◽

Stéphane Bonacorsi ◽

Naima Brahimi ◽

Olivier Clermont ◽

Xavier Nassif ◽

...

Keyword(s):

Escherichia Coli ◽

Rat Model ◽

Trna Gene ◽

Bactericidal Effect ◽

Neonatal Rat ◽

Neonatal Meningitis ◽

E Coli ◽

High Level ◽

Lower Capacity ◽

Neonatal Rat Model

ABSTRACT We report that the archetypal Escherichia coli strain C5 causing neonatal meningitis harbors a pathogenicity island (PAI) designated PAI IC5 that is similar to the PAI IIJ96 of uropathogenic E. coli J96 inserted in the leuX-tRNA gene. PAI-negative C5 mutants had a lower capacity than C5 to induce high-level bacteremia in a neonatal rat model. However, no change in their resistance to the bactericidal effect of serum and their capacity to cross the blood-brain barrier was observed.

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In vivo Analysis of the Resistance of the Meshes to Escherichia coli Infection

Frontiers in Surgery ◽

10.3389/fsurg.2021.644227 ◽

2021 ◽

Vol 8 ◽

Author(s):

Xinsen Xu ◽

Ming Zhan ◽

Xinxing Li ◽

Tao Chen ◽

Linhua Yang

Keyword(s):

Escherichia Coli ◽

Antibiotic Prophylaxis ◽

Rat Model ◽

Polypropylene Mesh ◽

Bacterial Resistance ◽

Giant Cells ◽

Biologic Mesh ◽

Mesh Infection ◽

E Coli

Background: The mesh infection is mostly related to the gram-negative bacteria, such as Escherichia coli (E. coli) for emergency surgery of incarcerated hernia. However, few study investigated the effects of E. coli concentration, mesh materials and antibiotic prophylaxis on mesh infection after hernioplasty. The aim of this study was to evaluate the bacterial resistance to E. coli for three different materials of mesh, and to measure the minimum E. coli concentration for mesh infection with and without antibiotic prophylaxis in a rat model.Methods: Three types of mesh (polytetrafluoroethylene, polypropylene, and biologic meshes) were used in the repair of an acute ventral hernia rat model in the setting of different concentrations of E. coli loads and antibiotics. At the 8th day after surgery, mesh samples were sent for microbiologic and histologic analyses.Results: The positive rates of bacterial culture increased with E. coli concentration. The biologic mesh showed better bacterial resistance compared to polytetrafluoroethylene mesh and polypropylene mesh when the concentration of E. coli ranges from 106 CFU/ml to 108 CFU/ml (P = 0.002 and P = 0.029, respectively). Prophylactical ceftriaxone treatment could not decrease the colonization rate of E. coli at 106 CFU/ml or 108 CFU/ml in each group (P > 0.05). The scores of neovascularization in polypropylene mesh and biologic mesh were similar, which was higher than that of polytetrafluoroethylene mesh (P < 0.05). Compared with other meshes, biologic mesh showed better tolerance to 106 CFU/ml E. coli with respect to inflammation, depth of inflammation, neovascularization, cellular repopulation and foreign body giant cells.Conclusion: The biologic mesh had better E. coli resistance compared to polytetrafluoroethylene mesh and polypropylene mesh when the E. coli concentration is higher than 106 CFU/ml in rats. Antibiotic prophylaxis was useful when the contamination was not particularly severe.

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Synergistic effect of a recombinant N-terminal fragment of bactericidal/permeability-increasing protein and cefamandole in treatment of rabbit gram-negative sepsis.

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.40.1.65 ◽

1996 ◽

Vol 40 (1) ◽

pp. 65-69 ◽

Cited By ~ 31

Author(s):

Y Lin ◽

W J Leach ◽

W S Ammons

Keyword(s):

Escherichia Coli ◽

Septic Shock ◽

Combined Treatment ◽

Bacterial Clearance ◽

Gram Negative ◽

E Coli ◽

Terminal Fragment ◽

Cephalosporin Antibiotic

As a consequence of their bactericidal actions, many antibiotics cause the release of endotoxin, a primary mediator of gram-negative sepsis. Bactericidal/permeability-increasing protein (BPI) has bactericidal activity and neutralizes endotoxin in vitro and in vivo. We sought to examine the effect of a recombinant N-terminal fragment of BPI (rBPI21) in conjunction with cefamandole, a cephalosporin antibiotic, in the treatment of Escherichia coli bacteremia and septic shock in rabbits. Cefamandole (100 mg/kg of body weight) was injected intravenously. This was followed by simultaneous 10-min infusions of E. coli O7:K1 (9 x 10(9) CFU/kg) and rBPI21 (10 mg/kg). rBPI21 was continuously infused for an additional 110 min at 10 mg/kg/h. The administration of rBPI21 in conjunction with the administration of cefamandole prevented the cefamandole-induced increase of free endotoxin in plasma, accelerated bacterial clearance, ameliorated cardiopulmonary dysfunction, and thereby, prevented death, whereas neither agent alone was protective in this animal model. The efficacy of the combined treatment with rBPI21 and cefamandole suggests a synergistic interaction between the two agents. The data indicate that rBPI21 may be useful in conjunction with traditional antibiotic therapy.

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Lack of protection against ascending Escherichia coli pyelonephritis in diabetic rats following immunization with purified lipopolysaccharide

Canadian Journal of Microbiology ◽

10.1139/m86-179 ◽

1986 ◽

Vol 32 (12) ◽

pp. 967-969

Author(s):

L. E. Bryan ◽

T. Schollaardt ◽

C. Y. Pak ◽

C. J. Kim ◽

J. W. Yoon

Keyword(s):

Escherichia Coli ◽

Virulence Factors ◽

Rat Model ◽

Specific Antibody ◽

Diabetic Rats ◽

Diabetic Rat ◽

Potential Contribution ◽

E Coli ◽

Protective Antigens ◽

Diabetic Rat Model

Purified lipopolysaccharide of Escherichia coli produced specific antibody when injected intraperitoneally or given to rats orally. Either route of immunization did not prevent ascending pyelonephritis in a diabetic rat model. The use of purified LPS excludes the potential contribution of other virulence factors of E. coli as protective antigens in the prevention of ascending pyelonephritis and confirms that anti-lipopolysaccharide antibody is not protective for ascending pyelonephritis.

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Correlation between electrophoretic types B1and B2of carboxylesterase B and host-dependent factors inEscherichia colisepticaemia

Epidemiology and Infection ◽

10.1017/s0950268800065559 ◽

1988 ◽

Vol 100 (1) ◽

pp. 51-61 ◽

Cited By ~ 17

Author(s):

B. Picard ◽

Ph. Goullet

Keyword(s):

Escherichia Coli ◽

Septic Shock ◽

Underlying Disease ◽

Lower Proportion ◽

Type B ◽

E Coli ◽

Underlying Illness ◽

Male Patients ◽

Underlying Diseases ◽

Compromised Patients

SUMMARYElectrophoretic types B1and B2of carboxylesterase B produced by strains ofEscherichia coliisolated from 100 septicaemia cases were correlated with α-haemolysin and mannose resistant haemagglutinin (MRHA) production and with clinical data including eventual underlying diseases, origin of septicaemia and evolution. Electrophoretic type B2was phenotypically linked with α-haemolysin and MRHA production. The proportion of type B2isolates varied significantly with occurrence of an underlying illness (45% for patients without an underlying disease and 22% for compromised patients) and with the site of origin of the septicaemia (40% for those of urinary origin and 18% for infection of digestive origin). In the former infections, type B2isolates were obtained in the majority from male patients while type B1isolates predominated in women. The septicaemias associated with type B1were characterized by a lower proportion of isolates producing α-haemolysin and MRHA and by a greater frequency of septic shock and death than those associated with type B2. These facts emphasize the importance of host-dependent factors inE. colisepticaemia.

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Fatal Necrotising Soft Tissue Infection Caused by Escherichia coli in Alcoholic Liver Cirrhosis

10.22541/au.162244172.24758400/v1 ◽

2021 ◽

Author(s):

MIO KURIMOTO ◽

Mahoko Ikeda ◽

Ichiro Hirayama ◽

Yatsuya Kobayashi ◽

Mio Shikama ◽

...

Keyword(s):

Escherichia Coli ◽

Septic Shock ◽

Liver Cirrhosis ◽

Host Susceptibility ◽

Alcoholic Liver Cirrhosis ◽

Severe Condition ◽

E Coli ◽

Pathogen Virulence ◽

Susceptibility Factors ◽

Monomicrobial Infection

NSTI caused by E. coli monomicrobial infection is an extremely severe condition with high mortality. This report presented a case of monomicrobial NSTI caused by E. coli with septic shock in a patient with alcoholic liver cirrhosis and mentioned both pathogen virulence factors and host susceptibility factors.

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Release of interleukin-12 in experimental Escherichia coli septic shock in baboons: relation to plasma levels of interleukin-10 and interferon- gamma

Blood ◽

10.1182/blood.v87.12.5144.bloodjournal87125144 ◽

1996 ◽

Vol 87 (12) ◽

pp. 5144-5151 ◽

Cited By ~ 57

Author(s):

PM Jansen ◽

TC van der Pouw Kraan ◽

IW de Jong ◽

G van Mierlo ◽

J Wijdenes ◽

...

Keyword(s):

Escherichia Coli ◽

Septic Shock ◽

Interferon Gamma ◽

Biologically Active ◽

Sublethal Dose ◽

Interleukin 12 ◽

Ifn Gamma ◽

E Coli ◽

Enhanced Production

Interleukin (IL)-12 is thought to be a key factor for the induction of interferon gamma (IFN-gamma), a cytokine essential for the lethal effects of endotoxin. We report here on the release of the nonfunctional subunit of IL-12, p40, as well as biologically active heterodimeric IL-12, p70, after administration of a lethal (n = 5) or sublethal (n = 8) dose of live Escherichia coli to baboons. Remarkably, on lethal challenge, peak levels of p40 were observed at 3 hours that were about twofold lower than those elicited after sublethal challenge (2,813 +/- 515 pg/mL v 4,972 +/- 732 pg/mL, P < .05). This disparity was also observed, although to a lesser extent, for IL-12 p70 antigen, of which maximum levels of 91 +/- 47 pg/mL and 151 +/- 41 pg/mL were measured 6 hours after a lethal or sublethal dose of E coli, respectively. Circulating p70 antigen correlated with IL-12 biologic activity (r = 0.869; P < .001). When comparing lethal to sublethal conditions, lower peak levels of IL-12 on lethal E coli sharply contrasted with higher levels of other proinflammatory cytokines, such as tumor necrosis factor (TNF)-alpha, IL-1beta, IL-6, and IL-8 observed in these animals. Lower IL-12 concentrations in the lethal group may have resulted in part from the enhanced production of IL-10, a known inhibitor of IL-12 synthesis in vitro, as peak levels of this cytokine 3 hours postchallenge inversely correlated with peak levels of IL-12, in particular p40 (r = -0.802; P < .01). Contrary to what might be expected if IFN-gamma were solely induced by IL-12, lethally challenged baboons generated threefold more IFN-gamma at 6 hours than those receiving a sublethal dose (P < .05). Moreover, higher levels of IFN- gamma were associated with lower p40/p70 ratios, suggesting that, in agreement with observations in vitro, IFN-gamma may have preferentially upregulated the release of p70 over p40. These data show that IL-12 is released in experimental septic shock in nonhuman primates and suggest that IL-10 and IFN-gamma are involved in the regulation of this release. Furthermore, this study indicates that the systemic release of IL-12 might be essential, but is not likely sufficient, to promote lethal production of IFN-gamma in sepsis.

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Avian-Pathogenic Escherichia coli Strains Are Similar to Neonatal Meningitis E. coli Strains and Are Able To Cause Meningitis in the Rat Model of Human Disease

Infection and Immunity ◽

10.1128/iai.00347-10 ◽

2010 ◽

Vol 78 (8) ◽

pp. 3412-3419 ◽

Cited By ~ 104

Author(s):

Kelly A. Tivendale ◽

Catherine M. Logue ◽

Subhashinie Kariyawasam ◽

Dianna Jordan ◽

Ashraf Hussein ◽

...

Keyword(s):

Escherichia Coli ◽

Rat Model ◽

Neonatal Meningitis ◽

Zoonotic Potential ◽

E Coli ◽

Virulence Plasmids ◽

Pathogenic Escherichia Coli ◽

Typing Methods ◽

Tract Infections

ABSTRACT Escherichia coli strains causing avian colibacillosis and human neonatal meningitis, urinary tract infections, and septicemia are collectively known as extraintestinal pathogenic E. coli (ExPEC). Characterization of ExPEC strains using various typing techniques has shown that they harbor many similarities, despite their isolation from different host species, leading to the hypothesis that ExPEC may have zoonotic potential. The present study examined a subset of ExPEC strains: neonatal meningitis E. coli (NMEC) strains and avian-pathogenic E. coli (APEC) strains belonging to the O18 serogroup. The study found that they were not easily differentiated on the basis of multilocus sequence typing, phylogenetic typing, or carriage of large virulence plasmids. Among the APEC strains examined, one strain was found to be an outlier, based on the results of these typing methods, and demonstrated reduced virulence in murine and avian pathogenicity models. Some of the APEC strains tested in a rat model of human neonatal meningitis were able to cause meningitis, demonstrating APEC's ability to cause disease in mammals, lending support to the hypothesis that APEC strains have zoonotic potential. In addition, some NMEC strains were able to cause avian colisepticemia, providing further support for this hypothesis. However, not all of the NMEC and APEC strains tested were able to cause disease in avian and murine hosts, despite the apparent similarities in their known virulence attributes. Thus, it appears that a subset of NMEC and APEC strains harbors zoonotic potential, while other strains do not, suggesting that unknown mechanisms underlie host specificity in some ExPEC strains.

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