Calcium Antagonistic Effects of an Angiographic Contrasi Medium In Vitro

1995 ◽  
Vol 30 (1) ◽  
pp. 21-27 ◽  
Author(s):  
JENS KARSTOFT ◽  
LARS B????TH ◽  
INGER JANSEN ◽  
LARS EDVINSSON
Keyword(s):  
Antagonistic Effects

scholarly journals Isolation of lactic acid bacteria from swine milk and characterization of potential probiotic strains with antagonistic effects against swine-associated gastrointestinal pathogens

2016 ◽  
Vol 62 (6) ◽  
pp. 514-524 ◽  
Author(s):  
Sandra Rayén Quilodrán-Vega ◽  
Julio Villena ◽  
José Valdebenito ◽  
María José Salas ◽  
Cristian Parra ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Human Milk ◽  
Salmonella Enterica ◽  
Enterohemorrhagic Escherichia Coli ◽  
Salmonella Infection ◽  
Antagonistic Effects ◽  
Gastrointestinal Pathogens

Probiotics are usually isolated from the gastrointestinal tract of humans and animals. The search of probiotics in human milk is a recent field of research, as the existence of the human milk microbiome was discovered only about a decade ago. To our knowledge, no reports regarding the potential probiotic effect of bacteria from swine milk have been published. In this work, we isolated several lactic acid bacteria from swine milk and evaluated them for them potential as probiotics. Among the isolated strains, Lactobacillus curvatus TUCO-5E showed antagonistic effects against swine-associated gastrointestinal pathogens. TUCO-5E was able to reduce the growth of enterotoxigenic and enterohemorrhagic Escherichia coli strains as well as pathogenic salmonella. In vitro exclusion and displacement assays in intestinal epithelial cells showed a remarkable antagonistic effect for L. curvatus TUCO-5E against Salmonella sp. strain TUCO-I7 and Salmonella enterica ATCC 13096. Moreover, by using a mouse model of Salmonella infection, we were able to demonstrate that preventative administration of L. curvatus TUCO-5E for 5 consecutive days was capable of decreasing the number of Salmonella enterica serovar Typhimurium in the liver and spleen of treated mice, compared with the controls, and prevented dissemination of the pathogen to the blood stream. Therefore, we have demonstrated here that swine milk is an interesting source of beneficial bacteria. In addition, the results of this work suggest that L. curvatus TUCO-5E is a good candidate to study in vivo the protective effect of probiotics against intestinal infection and damage induced by Salmonella infection in the porcine host.

scholarly journals In Vitro Potentiation of Antibiotic Activities by a Catecholate Iron Chelator against Chloroquine-Resistant Plasmodium falciparum

2002 ◽  
Vol 46 (1) ◽  
pp. 225-228 ◽  
Author(s):  
Bruno Pradines ◽  
Florence Ramiandrasoa ◽  
Jean Marc Rolain ◽  
Christophe Rogier ◽  
Joel Mosnier ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Iron Chelator ◽  
Synergistic Activity ◽  
Antagonistic Effects

ABSTRACT FR160, a catechol iron chelator, and tetracyclines or norfloxacin exert in vitro additive or synergistic activity against a chloroquine-resistant Plasmodium falciparum clone. FR160 shows antagonistic effects in association with macrolides, ofloxacin, and rifampin.

scholarly journals In Vitro Activity of Terbinafine Combined with Caspofungin and Azoles against Pythium insidiosum

2009 ◽  
Vol 53 (5) ◽  
pp. 2136-2138 ◽  
Author(s):  
Ayrton S. Cavalheiro ◽  
Grazieli Maboni ◽  
Maria I. de Azevedo ◽  
Juliana S. Argenta ◽  
Daniela I. B. Pereira ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Pythium Insidiosum ◽  
Antagonistic Effects ◽  
Antifungal Activities ◽  
Synergistic Interactions

ABSTRACT In this text we evaluated the in vitro antifungal activities of terbinafine combined with caspofungin, miconazole, ketoconazole, and fluconazole against 17 Pythium insidiosum strains by using the microdilution checkerboard method. Synergistic interactions were observed with terbinafine combined with caspofungin (41.2% of the strains), fluconazole (41.2%), ketoconazole (29.4%), and miconazole (11.8%). No antagonistic effects were observed. The combination of terbinafine plus caspofungin or terbinafine plus fluconazole may have significant therapeutic potential for treatment of pythiosis.

scholarly journals Trifluoperazine, a calmodulin antagonist, inhibits muscle cell fusion.

1983 ◽  
Vol 97 (5) ◽  
pp. 1375-1380 ◽  
Author(s):  
D Bar-Sagi ◽  
J Prives
Keyword(s):  
Acetylcholine Receptor ◽  
Muscle Cell ◽  
Myoblast Fusion ◽  
Ionophore A23187 ◽  
Calmodulin Antagonists ◽  
Calmodulin Antagonist ◽  
Antagonistic Effects ◽  
Skeletal Myoblasts ◽  
Temporally Correlated

We investigated the effect of trifluoperazine (TFP), a calmodulin antagonist, on the fusion of chick skeletal myoblasts in culture. TFP was found to inhibit myoblast fusion. This effect occurs at concentrations that have been reported to inhibit Ca2+-calmodulin in vitro, and is reversed upon removal of TFP. In addition, other calmodulin antagonists, including chlorpromazine, N-(6-aminohexyl)-5-chloro-1-naphthalene-sulfonamide (W7), and N-(6-aminohexyl)-1-naphthalene-sulfonamide (W5), inhibit fusion at doses that correspond closely to the antagonistic effects of these drugs on calmodulin. The expression of surface acetylcholine receptor, a characteristic aspect of muscle differentiation, is not impaired in TFP-arrested myoblasts. Myoblasts inhibited from fusion by 10 microM TFP display impaired alignment. In the presence of the Ca2+ ionophore A23187, the fusion block by 10 microM TFP is partially reversed and myoblast alignment is restored. The presence and distribution of calmodulin in both prefusional myoblasts and fused muscle cells was established by immunofluorescence. We observed an apparent redistribution of calmodulin staining that is temporally correlated with the onset of myoblast fusion. Our findings suggest a possible role for calmodulin in the regulation of myoblast fusion.

Antagonistic effects of FR 173657 on human, pig, rabbit, and guinea pig kinin receptors: an in vitro study

1997 ◽  
Vol 75 (6) ◽  
pp. 601-608 ◽  
Author(s):  
A Rizzi ◽  
F Gobeil ◽  
G Bogoni ◽  
G Calò ◽  
C Campobasso ◽  
...  
Keyword(s):  
Guinea Pig ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antagonistic Effects ◽  
Kinin Receptors

An in vitro examination of selenium–cadmium antagonism using primary cultures of rainbow trout (Oncorhynchus mykiss) hepatocytes

Metallomics ◽  
2016 ◽  
Vol 8 (2) ◽  
pp. 218-227 ◽  
Author(s):  
Ankur Jamwal ◽  
Mohammad Naderi ◽  
Som Niyogi
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Primary Cultures ◽  
Ros Generation ◽  
Antagonistic Effects ◽  
Low Concentration ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Dose Dependent ◽  
In Vitro Examination

Se has antagonistic effects on Cd-induced cytotoxicityviaboth enzymatic and non-enzymatic antioxidative mechanisms and the effects are strictly dose dependent. Confocal fluorescent images of isolated rainbow trout hepatocytes exposed to 100 µM Cd, alone or in combination with low (25 µM) or high (250 µM) concentration of Se, show reduced ROS generation with low concentration of Se.

The antagonistic properties of Trichoderma spp. inhabiting woods for potential biological control of wood-damaging fungi

Holzforschung ◽  
2012 ◽  
Vol 66 (7) ◽  
pp. 883-887 ◽  
Author(s):  
Jaejung Lee ◽  
Nayoon Huh ◽  
Joo Hyun Hong ◽  
Beom Seok Kim ◽  
Gyu-Hyeok Kim ◽  
...  
Keyword(s):  
Biological Control ◽  
Chitinase Activity ◽  
Dual Culture ◽  
Trichoderma Spp ◽  
Trichoderma Longibrachiatum ◽  
Antagonistic Effects ◽  
Volatile Metabolites ◽  
Antagonistic Potential ◽  
Trichoderma Aureoviride

Abstract The antagonistic potential of Trichoderma spp. for biological control of wood-damaging fungi was investigated in the present paper. In vitro assays to investigate antifungal characteristics of Trichoderma spp. were conducted with various wood-damaging fungi. Exo-chitinase activity of the isolates was also measured. Three typical wood decayers and three sap-stainers served as target fungi. The antagonistic abilities of each Trichoderma species differed markedly according to the target fungus. The growth inhibition rates shown by the non-volatile metabolites against the wood decayers reached 100% for Trichoderma harzianum KUC1459. The antibiotics produced by Trichoderma dorotheae KUC5027, a recently reported species of Trichoderma, revealed strong antagonistic effects against sap-stainers. Trichoderma gamsii KUC1747 effectively inhibited the growth of all wood-damaging fungi in dual culture tests. The exo-chitinases of Trichoderma longibrachiatum KUC1540, Trichoderma aureoviride KUC1335, and T. harzianum KUC1459 showed significantly high activity.

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