Molecular detection of respiratory syncytial virus in postmortem lung tissue samples from Mexican children deceased with pneumonia

2001 ◽  
Vol 20 (5) ◽  
pp. 495-501 ◽  
Author(s):  
MARIA ELENA BUSTAMANTE-CALVILLO ◽  
F. RAÚL VELÁZQUEZ ◽  
LOURDES CABRERA-MUÑOZ ◽  
JAVIER TORRES ◽  
ALEJANDRO GÓMEZ-DELGADO ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Lung Tissue ◽  
Molecular Detection ◽  
Tissue Samples ◽  
Mexican Children ◽  
Syncytial Virus

Subgrouping of bovine respiratory syncytial virus strains detected in lung tissue

1996 ◽  
Vol 53 (3-4) ◽  
pp. 253-260 ◽  
Author(s):  
R.S. Schrijver ◽  
F. Daus ◽  
J.A. Kramps ◽  
J.P.M. Langedijk ◽  
R. Buijs ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Lung Tissue ◽  
Bovine Respiratory Syncytial Virus ◽  
Syncytial Virus ◽  
Virus Strains

scholarly journals Prevalence of respiratory viruses using polymerase chain reaction in children with wheezing, a systematic review and meta–analysis

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243735
Author(s):  
Cyprien Kengne–Nde ◽  
Sebastien Kenmoe ◽  
Abdou Fatawou Modiyinji ◽  
Richard Njouom
Keyword(s):  
Systematic Review ◽  
Polymerase Chain Reaction ◽  
Respiratory Syncytial Virus ◽  
Molecular Detection ◽  
Respiratory Viruses ◽  
Human Bocavirus ◽  
Chain Reaction ◽  
Manual Search ◽  
Polymerase Chain ◽  
Syncytial Virus

Introduction Wheezing is a major problem in children, and respiratory viruses are often believed to be the causative agent. While molecular detection tools enable identification of respiratory viruses in wheezing children, it remains unclear if and how these viruses are associated with wheezing. The objective of this systematic review is to clarify the prevalence of different respiratory viruses in children with wheezing. Methods We performed an electronic in Pubmed and Global Index Medicus on 01 July 2019 and manual search. We performed search of studies that have detected common respiratory viruses in children ≤18 years with wheezing. We included only studies using polymerase chain reaction (PCR) assays. Study data were extracted and the quality of articles assessed. We conducted sensitivity, subgroup, publication bias, and heterogeneity analyses using a random effects model. Results The systematic review included 33 studies. Rhinovirus, with a prevalence of 35.6% (95% CI 24.6–47.3, I2 98.4%), and respiratory syncytial virus, at 31.0% (95% CI 19.9–43.3, I2 96.4%), were the most common viruses detected. The prevalence of other respiratory viruses was as follows: human bocavirus 8.1% (95% CI 5.3–11.3, I2 84.6%), human adenovirus 7.7% (95% CI 2.6–15.0, I2 91.0%), influenza virus6.5% (95% CI 2.2–12.6, I2 92.4%), human metapneumovirus5.8% (95% CI 3.4–8.8, I2 89.0%), enterovirus 4.3% (95% CI 0.1–12.9, I2 96.2%), human parainfluenza virus 3.8% (95% CI 1.5–6.9, I2 79.1%), and human coronavirus 2.2% (95% CI 0.6–4.4, I2 79.4%). Conclusions Our results suggest that rhinovirus and respiratory syncytial virus may contribute to the etiology of wheezing in children. While the clinical implications of molecular detection of respiratory viruses remains an interesting question, this study helps to illuminate the potential of role respiratory viruses in pediatric wheezing. Review registration PROSPERO, CRD42018115128.

Influence of preanalytical parameters on influenza and respiratory syncytial virus molecular detection

2022 ◽  
pp. 105063
Author(s):  
Claire Grolhier ◽  
Charlotte Pronier ◽  
Zahida Mamou ◽  
Christopher Heslan ◽  
Vincent Thibault
Keyword(s):  
Respiratory Syncytial Virus ◽  
Molecular Detection ◽  
Syncytial Virus

scholarly journals Analysis of lung transcriptome in calves infected with Bovine Respiratory Syncytial Virus and treated with antiviral and/or cyclooxygenase inhibitor

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0246695
Author(s):  
Maxim Lebedev ◽  
Heather A. McEligot ◽  
Victoria N. Mutua ◽  
Paul Walsh ◽  
Francisco R. Carvallo Chaigneau ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Respiratory Syncytial Virus ◽  
Differential Gene Expression ◽  
Lung Tissue ◽  
Bovine Respiratory Syncytial Virus ◽  
Pathway Enrichment Analysis ◽  
Adaptive Immune ◽  
Differential Gene ◽  
Syncytial Virus

Bovine Respiratory Syncytial virus (BRSV) is one of the major infectious agents in the etiology of the bovine respiratory disease complex. BRSV causes a respiratory syndrome in calves, which is associated with severe bronchiolitis. In this study we describe the effect of treatment with antiviral fusion protein inhibitor (FPI) and ibuprofen, on gene expression in lung tissue of calves infected with BRSV. Calves infected with BRSV are an excellent model of human RSV in infants: we hypothesized that FPI in combination with ibuprofen would provide the best therapeutic intervention for both species. The following experimental treatment groups of BRSV infected calves were used: 1) ibuprofen day 3–10, 2) ibuprofen day 5–10, 3) placebo, 4) FPI day 5–10, 5) FPI and ibuprofen day 5–10, 6) FPI and ibuprofen day 3–10. All calves were infected with BRSV on day 0. Daily clinical evaluation with monitoring of virus shedding by qRT-PCR was conducted. On day10 lung tissue with lesions (LL) and non-lesional (LN) was collected at necropsy, total RNA extracted, and RNA sequencing performed. Differential gene expression analysis was conducted with Gene ontology (GO) and KEGG pathway enrichment analysis. The most significant differential gene expression in BRSV infected lung tissues was observed in the comparison of LL with LN; oxidative stress and cell damage was especially noticeable. Innate and adaptive immune functions were reduced in LL. As expected, combined treatment with FPI and Ibuprofen, when started early, made the most difference in gene expression patterns in comparison with placebo, especially in pathways related to the innate and adaptive immune response in both LL and LN. Ibuprofen, when used alone, negatively affected the antiviral response and caused higher virus loads as shown by increased viral shedding. In contrast, when used with FPI Ibuprofen enhanced the specific antiviral effect of FPI, due to its ability to reduce the damaging effect of prostanoids and oxidative stress.

scholarly journals Infected Cell Types in Ovine Lung Following Exposure to Bovine Respiratory Syncytial Virus

1994 ◽  
Vol 31 (2) ◽  
pp. 229-236 ◽  
Author(s):  
J. T. Meehan ◽  
R. C. Cutlip ◽  
H. D. Lehmkuhl ◽  
J. P. Kluge ◽  
M. R. Ackermann
Keyword(s):  
Respiratory Syncytial Virus ◽  
Lymph Nodes ◽  
Lung Tissue ◽  
Viral Antigen ◽  
Bovine Respiratory Syncytial Virus ◽  
Post Inoculation ◽  
Type I ◽  
Mediastinal Lymph Nodes ◽  
Bronchiolar Epithelium ◽  
Syncytial Virus

Sixteen adult sheep (ten females, six males obtained from a closed flock at National Animal Disease Center, Ames, IA) were experimentally infected with bovine respiratory syncytial virus strain 375 (BRSV), and lung tissues were stained for viral antigen. Two infected sheep were euthanatized at each of the following post-inoculation times: 12, 24, 36, 48, 72, 96, 144, and 192 hours. Lung, nasal turbinates, trachea, right cranial bronchial and mediastinal lymph nodes, liver, and spleen were collected for histologic evaluation. An indirect immunoperoxidase technique was performed on routine paraffin-embedded sections of lung tissue, trachea, turbinates, and bronchial and mediastinal lymph nodes to determine the location of the BRSV antigen. For lung tissue from each sheep 400 light microscopic fields at 160x magnification were examined for staining for BRSV antigen. Lung tissue was also collected for virus and bacterial isolation. Daily serum samples were taken for determination of anti-BRSV titers. Severe respiratory disease was not produced in any sheep. Bovine respiratory syncytial virus was isolated from lung tissue collected from all sheep up through 144 hours postinoculation. At 12 hours post-inoculation (case No. 2) respiratory syncytial virus antigen was detected in bronchiolar epithelium and a mononuclear cell within an alveolar space. Lung tissue from the sheep necropsied between 24 and 144 hours postinoculattion (case Nos. 3–14) contained BRSV antigen in bronchiolar epithelium, type I pneumocytes, type II pneumocytes. alveolar macrophages, and mononuclear cells within alveolar spaces. Macrophages staining for viral antigen were rare. Bronchiolar and type I epithelial cells comprised the majority of infected cells. In a separate experiment, lung slices inoculated in vitro with either BRSV or ovine adenovirus did not stain for the respective antigens. Slices inoculated with parainfluenzavirus-3 did stain for that viral antigen.

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