A New Culture System to Study the Metabolism of the Intervertebral Disc In Vitro

Spine ◽  
1998 ◽  
Vol 23 (17) ◽  
pp. 1821-1827 ◽  
Author(s):  
Kazuhiro Chiba ◽  
Gunnar B. J. Andersson ◽  
Koichi Masuda ◽  
Shigeki Momohara ◽  
James M. Williams ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Culture System

Establishment of a Novel Intervertebral Disc/Endplate Culture Model: Analysis of an Ex Vivo In Vitro Whole-Organ Rabbit Culture System

Spine ◽  
2006 ◽  
Vol 31 (25) ◽  
pp. 2918-2925 ◽  
Author(s):  
Daniel Haschtmann ◽  
Jivko V. Stoyanov ◽  
Ladina Ettinger ◽  
Lutz -P. Nolte ◽  
Stephen J. Ferguson
Keyword(s):  
Intervertebral Disc ◽  
Culture System ◽  
Ex Vivo ◽  
Model Analysis ◽  
Culture Model

Organ Culture System for Mechanobiology Studies of the Intervertebral Disc

2004 ◽  
Author(s):  
Cynthia R. Lee ◽  
Mauro Alini ◽  
James C. Iatridis
Keyword(s):  
Tissue Engineering ◽  
Intervertebral Disc ◽  
Culture System ◽  
Static Load ◽  
In Vitro Models ◽  
Disc Regeneration ◽  
Organ Culture System ◽  
In Vitro Culture System ◽  
Vertebral Endplates

The development of in vitro models is critical for furthering understanding of the intervertebral disc and the development of disc regeneration/tissue engineering. An in vitro culture system targeted towards mechano-biology studies of the intervertebral disc (IVD) was built and validated using bovine coccygeal discs. Discs were maintained in culture for up to one week with and without vertebral endplates. Water content and glycosaminoglycan content were found to be stable and cells were metabolically active when cultured under a 5kg static load.

scholarly journals Characterization of an in vitro intervertebral disc organ culture system

2007 ◽  
Vol 16 (7) ◽  
pp. 1029-1037 ◽  
Author(s):  
Casey L. Korecki ◽  
Jeffrey J. MacLean ◽  
James C. Iatridis
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Culture System ◽  
Organ Culture System

Effect of TGF-β3 on the Gene Expression of Intervertebral Disc Cells in 3D Pellet Cultures

2010 ◽  
Author(s):  
C. Houseman ◽  
M. Scro ◽  
S. Belverud ◽  
D. Chen ◽  
P. Razzano ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Tissue Regeneration ◽  
Annulus Fibrosus ◽  
Culture System ◽  
Tissue Repair ◽  
Tissue Type ◽  
Disc Cells ◽  
Ivd Degeneration

Intervertebral disc (IVD) degeneration typically involves changes in the multiple constitutive tissues of the IVD. Many tissue repair efforts have focused on the use of differentiated disc cells or stem cells for the regeneration of an IVD in vitro. Consequently, successful long term culture of human disc cells is pivotal for tissue regeneration of the IVD. The aim of this study is to establish a long-term in vitro culture system for the growth of disc cells that maintain their phenotype based on the anatomical origin (annulus fibrosus (AF), nucleus pulposus (NP), or the vertebral end-plates (EP)). This maintenance of phenotype is crucial for examination of treatment efficacy, which is typically designed to induce regeneration of a single tissue type (i.e. injection of growth factors into the NP or anti-inflammatory treatment of the EPs).

Studies of Human Intervertebral Disc Cell Function in a Constrained In Vitro Tissue Culture System

Spine ◽  
2004 ◽  
Vol 29 (11) ◽  
pp. 1187-1195 ◽  
Author(s):  
Christine Lyn Le Maitre ◽  
Judith Alison Hoyland ◽  
Anthony J. Freemont
Keyword(s):  
Tissue Culture ◽  
Intervertebral Disc ◽  
Culture System ◽  
Cell Function ◽  
Disc Cell ◽  
Tissue Culture System ◽  
In Vitro Tissue Culture

Supplementation of c-type natriuretic peptide during in vitro growth period benefits the development of murine preantral follicles

Zygote ◽  
2020 ◽  
pp. 1-5
Author(s):  
Li Ang ◽  
Cao Haixia ◽  
Li Hongxia ◽  
Li Ruijiao ◽  
Guo Xingping ◽  
...  
Keyword(s):  
Natriuretic Peptide ◽  
Granulosa Cells ◽  
Culture System ◽  
Early Stage ◽  
Developmental Competence ◽  
Control Group ◽  
Follicle Development ◽  
Control Groups ◽  
Preantral Follicles

Summary The present study investigated the effects of c-type natriuretic peptide (CNP) on the development of murine preantral follicles during in vitro growth (IVG). Preantral follicles isolated from ovaries of Kunming mice were cultured in vitro. In the culture system, CNP was supplemented in the experimental groups and omitted in the control groups. In Experiment 1, CNP was only supplemented at the early stage and follicle development was evaluated. In Experiments 2 and 3, CNP was supplemented during the whole period of in vitro culture. In Experiment 2, follicle development and oocyte maturity were evaluated. In Experiment 3, follicle development and embryo cleavage after in vitro fertilization (IVF) were assessed. The results showed that in the control groups in all three experiments, granulosa cells migrated from within the follicle and the follicles could not reach the antral stage. In the experimental groups in all three experiments, no migration of granulosa cells was observed and follicle development was assessed as attaining the antral stage, which was significantly superior to that of the control group (P < 0.0001). Oocyte meiotic arrest was effectively maintained, hence giving good developmental competence. In conclusion, CNP supplementation in the culture system during IVG benefited the development of murine preantral follicles.

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