A Rapid and Specific Assay for the Determination of Lamotrigine in Human Plasma by Normal-Phase HPLC

1997 ◽  
Vol 19 (1) ◽  
pp. 100-107 ◽  
Author(s):  
Anna Bartoli ◽  
Roberto Marchiselli ◽  
Giuliana Gatti
Keyword(s):  
Human Plasma ◽  
Normal Phase ◽  
Specific Assay ◽  
Normal Phase Hplc

Extraction, Cleanup, and Quantitative Determination of Aflatoxins in Corn

1980 ◽  
Vol 63 (3) ◽  
pp. 631-633 ◽  
Author(s):  
James E Thean ◽  
David R Lorenz ◽  
David M Wilson ◽  
Kathleen Rodgers ◽  
Richard C Gueldner
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Quantitative Determination ◽  
Ammonium Sulfate ◽  
Silica Gel ◽  
Normal Phase ◽  
Aqueous Methanol ◽  
Normal Phase Hplc ◽  
Water Saturated

Abstract A method is proposed for extraction and cleanup of corn samples for the quantitation of 4 aflatoxins by high pressure liquid chromatography (HPLC). After aqueous methanol extraction, ammonium sulfate treatment, and partition of aflatoxins into chloroform, sample extracts are partially purified on Sep-Pak cartridges or small columns packed with HPLC grade silica; cleanup requires only 13 mL solvent/sample. Aflatoxins B1, B2, G1, and G2 in the purified extract are resolved in ca 10 min by normal phase HPLC on a microparticulate (5 μm) silica gel column with a 50% water-saturated chloroform-cyclohexaneacetonitrile- ethanol solvent, and are measured by ultraviolet fluorescence in a silica gel-packed flowcell. Recoveries of added aflatoxins B1, B2, G1, and G2 were 84–118 % at levels of 1.5–125 μg/kg

Simultaneous determination of endogenous retinoic acid isomers and retinol in human plasma by isocratic normal-phase HPLC with ultraviolet detection

1994 ◽  
Vol 40 (1) ◽  
pp. 48-51 ◽  
Author(s):  
E Meyer ◽  
W E Lambert ◽  
A P De Leenheer
Keyword(s):  
Retinoic Acid ◽  
Human Plasma ◽  
Simple Procedure ◽  
Internal Standard ◽  
High Sensitivity ◽  
Normal Phase ◽  
Ultraviolet Detection ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid ◽  
Normal Phase Hplc

Abstract We describe a rapid and simple procedure for the simultaneous quantitation of endogenous 13-cis-retinoic acid, all-trans-retinoic acid, and retinol by isocratic normal-phase HPLC with ultraviolet detection, in 0.5 mL of human plasma. A silica adsorption column was eluted with n-hexane:2-propanol:acetic acid (200:0.7:0.135 by vol) at 0.9 mL/min, and the effluent monitored at 350 nm. The arotinoid ethylsulfonic acid Ro 15-1570 was used as the internal standard. High sensitivity, allowing quantitation of physiological concentrations, was achieved, particularly for the retinoic acid isomers. The detection limits were 0.5 microgram/L in plasma for both 13-cis- and trans-retinoic acid, and 10 micrograms/L for retinol. The CVs for between-day determinations of the lowest quality-control concentration (n = 12) were 4.8% for 13-cis-retinoic acid, 3.4% for trans-retinoic acid, and 3.0% for retinol. The mean (+/- SD) concentrations of 13-cis-retinoic acid (1.79 +/- 0.56 microgram/L), trans-retinoic acid (1.35 +/- 0.42 microgram/L), and retinol (533 +/- 58 micrograms/L) measured in plasma from 22 healthy volunteers agreed well with those previously reported.

Determination of vitamin A in liver and liver-containing products using narrow-bore normal, phase HPLC

1994 ◽  
Vol 199 (3) ◽  
pp. 206-209 ◽  
Author(s):  
Elisabeth Brinkmann ◽  
Irena Mehlitz ◽  
Henny Bijosono Oei ◽  
Reinhard Tiebach ◽  
Werner Baltes
Keyword(s):  
Vitamin A ◽  
Normal Phase ◽  
Normal Phase Hplc

Simple and Rapid Method for the Simultaneous Determination of Cholesterol and Retinol in Meat Using Normal-Phase HPLC Technique

2017 ◽  
Vol 11 (2) ◽  
pp. 319-326 ◽  
Author(s):  
Rubén Domínguez ◽  
Francisco J. Barba ◽  
Juan A. Centeno ◽  
Predrag Putnik ◽  
Hami Alpas ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Rapid Method ◽  
Normal Phase ◽  
Normal Phase Hplc ◽  
Hplc Technique
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