Expression of Heparanase Isoforms in Intervertebral Discs Classified According to Pfirrmann Grading System for Disc Degeneration

Spine ◽  
2013 ◽  
Vol 38 (13) ◽  
pp. 1112-1118 ◽  
Author(s):  
Luciano Miller Reis Rodrigues ◽  
Lilian Zerbinatti de Oliveira ◽  
Maria Aparecida da Silva Pinhal
Keyword(s):  
Disc Degeneration ◽  
Intervertebral Discs ◽  
Grading System

scholarly journals Validity and interobserver agreement of a new radiographic grading system for intervertebral disc degeneration: Part III. Thoracic spine

2021 ◽  
Author(s):  
Christian Liebsch ◽  
Youping Tao ◽  
Annette Kienle ◽  
Hans-Joachim Wilke
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Interobserver Agreement ◽  
Weighted Kappa ◽  
Intervertebral Discs ◽  
Height Loss ◽  
Grading System ◽  
Perfect Agreement ◽  
Radiographic Grading

Abstract Purpose The aim of this study was to assess the validity and objectivity of a new quantitative radiographic grading system for thoracic intervertebral disc degeneration. Methods The new grading system involves the measurement variables “Height loss” and “Osteophyte formation”, which are determined from lateral radiographs, resulting in the “Overall degree of degeneration” on a four-point scale from 0 (no degeneration) to 3 (severe degeneration). Validation was performed by comparing the radiographic degrees of degeneration of 54 human intervertebral discs to the respective macroscopic degrees, which were defined as the “real” degrees of degeneration. Interobserver agreement was examined using radiographs of 135 human thoracic intervertebral discs. Agreement was quantified by means of quadratically weighted Kappa coefficients with 95% confidence limits (CL). Results Validation revealed almost perfect agreement between the radiographic and the macroscopic overall degrees of degeneration (Kappa 0.968, CL 0.944–0.991), while the macroscopic grades tended to be underestimated in low degeneration grades. Radiographic grading of two independent observers also exhibited almost perfect agreement (Kappa 0.883, CL 0.824–0.941) as well as tendencies towards rater-dependent differences in low degeneration grades. Conclusion The new quantitative radiographic grading scheme represents a valid, reliable, and almost objective method for assessing the degree of degeneration of individual thoracic intervertebral discs. Potential effects of interindividual variations and the radiographic superimposition of anatomical structures represent a limitation of this method should be taken into account when using the grading system for clinical and experimental purposes, especially with regard to specific morphological as well as patient- and donor-specific characteristics.

scholarly journals Stem Cell Delivery with Polymer Hydrogel for Treatment of Intervertebral Disc Degeneration: From 3D Culture to Design of the Delivery Device for Minimally Invasive Therapy

2016 ◽  
Vol 25 (12) ◽  
pp. 2213-2220 ◽  
Author(s):  
Deepak Kumar ◽  
Alex Lyness ◽  
Irini Gerges ◽  
Christina Lenardi ◽  
Nicholas R. Forsyth ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Disc Degeneration ◽  
Mechanical Strain ◽  
3D Culture ◽  
Human Mesenchymal Stem Cells ◽  
Intervertebral Discs ◽  
Delivery Device ◽  
Polymer Hydrogel ◽  
Uv Curable ◽  
Stem Cell Delivery

Nucleus pulposus (NP) tissue damage can induce detrimental mechanical strain on the biomechanical performance of intervertebral discs (IVDs), causing subsequent disc degeneration. A novel, photocurable, injectable, synthetic polymer hydrogel (pHEMA-co-APMA grafted with PAA) has already demonstrated success in encapsulating and differentiating human mesenchymal stem cells (hMSCs) toward an NP phenotype during hypoxic conditions. After demonstration of promising results in our previous work, in this study we have further investigated the inclusion of mechanical stimulation and its impact on hMSC differentiation toward an NP phenotype through the characterization of matrix markers such as SOX-9, aggrecan, and collagen II. Furthermore, investigations were undertaken in order to approximate delivery parameters for an injection delivery device, which could be used to transport hMSCs suspended in hydrogel into the IVD. hMSC-laden hydrogel solutions were injected through various needle gauge sizes in order to determine its impact on postinjection cell viability and IVD tissue penetration. Interpretation of these data informed the design of a potential minimally invasive injection device, which could successfully inject hMSCs encapsulated in a UV-curable polymer into NP, prior to photo-cross-linking in situ.

scholarly journals Evaluation of apoptotic cell death in normal and chondrodystrophic canine intervertebral discs

2012 ◽  
Vol 2 (1) ◽  
pp. 6 ◽  
Author(s):  
Marie Klauser ◽  
Franck Forterre ◽  
Marcus Doherr ◽  
Andreas Zurbriggen ◽  
David Spreng ◽  
...  
Keyword(s):  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Annulus Fibrosus ◽  
Caspase 3 ◽  
Apoptotic Cell ◽  
Intervertebral Discs ◽  
Discogenic Pain ◽  
Age Related ◽  
Chondroid Metaplasia ◽  
Related Increase

Disc degeneration occurs commonly in dogs. A variety of factors is thought to contribute an inappropriate disc matrix that isolate cells in the disc and lead to apoptosis. Disc herniation with radiculopathy and discogenic pain are the results of the degenerative process. The objective of this prospective study was to determine the extent of apoptosis in intact and herniated intervertebral discs of chondrodystrophic dogs and non-chondrodystrophic dogs. In addition, the nucleus pulposus (NP) was histologically compared between non-chondrodystrophic and chondrodystrophic dogs. Thoracolumbar intervertebral discs and parts of the extruded nucleus pulposus were harvested from 45 dogs. Samples were subsequently stained with haematoxylin-eosin and processed to detect cleaved caspase-3 and poly(ADP-ribose) polymerase. A significant greater degree of apoptosis was observed in herniated NPs of chondrodystrophic dogs compared to non- chondrodystrophic dogs with poly (ADP-ribose) polymerase and cleaved caspase- 3 detection. Within the group of chondrodystrophic dogs, dogs with an intact disc and younger than 6 years showed a significant lower incidence of apoptosis in the NP compared to the herniated NP of chondrodystrophic dogs. The extent of apoptosis in the annulus fibrosus was not different between the intact disc from chondrodystrophic and non- chondrodystrophic dogs. An age-related increase of apoptotic cells in NP and annulus fibrosus was found in the intact non-herniated intervertebral discs. Histologically, absence of notochordal cells and occurrence of chondroid metaplasia were observed in the nucleus pulposus of chondrodystrophic dogs. As a result, we found that apoptosis plays a role in disc degeneration in chondrodystrophic dogs.

scholarly journals Validity and interobserver agreement of a new radiographic grading system for intervertebral disc degeneration: Part I. Lumbar spine

2005 ◽  
Vol 15 (6) ◽  
pp. 720-730 ◽  
Author(s):  
Hans-Joachim Wilke ◽  
Friederike Rohlmann ◽  
Cornelia Neidlinger-Wilke ◽  
Karin Werner ◽  
Lutz Claes ◽  
...  
Keyword(s):  
Lumbar Spine ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Interobserver Agreement ◽  
Grading System ◽  
Radiographic Grading

scholarly journals Validity and interobserver agreement of a new radiographic grading system for intervertebral disc degeneration: Part I. Lumbar spine

2006 ◽  
Vol 15 (6) ◽  
pp. 731-731 ◽  
Author(s):  
Hans-Joachim Wilke ◽  
Friederike Rohlmann ◽  
Cornelia Neidlinger-Wilke ◽  
Karin Werner ◽  
Lutz Claes ◽  
...  
Keyword(s):  
Lumbar Spine ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Interobserver Agreement ◽  
Grading System ◽  
Radiographic Grading

GENE THERAPY: A NOVEL APPROACH TO TREATMENT OF THE DEGENERATION OF INTERVERTEBRAL DISCS

2002 ◽  
Vol 06 (03n04) ◽  
pp. 181-187 ◽  
Author(s):  
Jiangwei Tan ◽  
Yougu Hu
Keyword(s):  
Gene Therapy ◽  
Back Pain ◽  
Disc Degeneration ◽  
Deep Understanding ◽  
Intervertebral Discs ◽  
Research Field ◽  
Clinical Approach ◽  
Novel Approach ◽  
Physiology And Biochemistry ◽  
Lowback Pain

The degeneration of intervertebral discs is one of the pathophysiological causes of lowback pain which has been seriously disturbing human health at the present, No clinical approach can provide prophylaxis or treatment for disc degeneration. But people are now thinking about gene transfer for the prevention of disc degeneration to reduce the incidence of back pain. This idea originated from the development of molecular biology; the deep understanding of the physiology and biochemistry of the intervertebral discs, and the growing scope of gene therapy. The increasing publications on gene therapy for disc degeneration have shown us the future of this research field, and revealed possible difficulties in the process of the study. This article focuses on several important aspects of gene therapy for disc degeneration.

Elderly female subjects are more likely to have narrowed disc space than the male subjects: A study in 359 elderly subjects using an 8-level MRI-based disc degeneration grading system

Bone ◽  
2010 ◽  
Vol 47 ◽  
pp. S449
Author(s):  
Yi-Xiang J. Wang ◽  
Anthony W.L. Kwok ◽  
James F. Griffith ◽  
Jason C.S. Leung ◽  
Heather T. Ma ◽  
...  
Keyword(s):  
Disc Degeneration ◽  
Elderly Subjects ◽  
Grading System ◽  
Disc Space ◽  
Elderly Female ◽  
Male Subjects ◽  
Female Subjects

Modified Pfirrmann Grading System for Lumbar Intervertebral Disc Degeneration

Spine ◽  
2007 ◽  
Vol 32 (24) ◽  
pp. E708-E712 ◽  
Author(s):  
James F. Griffith ◽  
Yi-Xiang J. Wang ◽  
Gregory E. Antonio ◽  
Kai Chow Choi ◽  
Alfred Yu ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Grading System ◽  
Lumbar Intervertebral Disc

scholarly journals Follistatin-Like 1 Attenuation Suppresses Intervertebral Disc Degeneration in Mice through Interacting with TNF-α and Smad Signaling Pathway

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Shaoyi Wang ◽  
Jianlu Wei ◽  
Jie Shi ◽  
Qiting He ◽  
Xiaocong Zhou ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Inflammatory Diseases ◽  
Morphological Changes ◽  
Intervertebral Discs ◽  
Inflammatory Factors ◽  
Wild Type ◽  
Tnf Α

Background. Inflammation plays an important role in intervertebral disc degeneration (IDD). The protein follistatin-like 1 (FSTL1) plays a proinflammatory role in a variety of inflammatory diseases. Objectives. The purpose of this study was to investigate whether IDD could be delayed by inhibiting FSTL-1 expression. Methods. We established a puncture-induced IDD model in wild-type and FSTL-1+/- mice and collected intervertebral discs (IVDs) from the mice. Safranin O staining was used to detect cartilage loss of IVD tissue, and HE staining was used to detect morphological changes of IVD tissue. We measured the expression of FSTL-1 and related inflammatory indicators in IVD tissues by immunohistochemical staining, real-time PCR, and Western blotting. Results. In the age-induced model of IDD, the level of FSTL-1 increased with the exacerbation of degeneration. In the puncture-induced IDD model, FSTL-1-knockdown mice showed a reduced degree of degeneration compared with that of wild-type mice. Further experiments showed that FSTL-1 knockdown also significantly reduced the level of related inflammatory factors in IVD. In vitro experiments showed that FSTL-1 knockdown significantly reduced TNF-α-induced inflammation. Specifically, the expression levels of the inflammatory factors COX-2, iNOS, MMP-13, and ADAMTS-5 were reduced. Knockdown of FSTL-1 attenuated inflammation by inhibiting the expression of P-Smad1/5/8, P-Erk1/2, and P-P65. Conclusion. Knockdown of FSTL-1 attenuated inflammation by inhibiting the TNF-α response and Smad pathway activity and ultimately delayed IDD.

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