Differential Pattern of Human Blood Neutrophil Activation After Stimulation With Organic Dust in Vitro and in Vivo

2007 ◽  
Vol 49 (2) ◽  
pp. 131-138 ◽  
Author(s):  
Eva Wikstr??m Jonsson ◽  
Lena Palmberg
Keyword(s):  
Human Blood ◽  
Neutrophil Activation ◽  
Blood Neutrophil ◽  
Organic Dust ◽  
Differential Pattern

scholarly journals Novel polyisoprenyl phosphates block phospholipase D and human neutrophil activation in vitro and murine peritoneal inflammation in vivo

2005 ◽  
Vol 146 (3) ◽  
pp. 344-351 ◽  
Author(s):  
Bruce D Levy ◽  
Lorraine Hickey ◽  
Andrew J Morris ◽  
Mykol Larvie ◽  
Raquel Keledjian ◽  
...  
Keyword(s):  
Phospholipase D ◽  
Human Neutrophil ◽  
Neutrophil Activation ◽  
Peritoneal Inflammation

scholarly journals Neutrophil activation in vitro and in vivo in Wegener's granulomatosis

1994 ◽  
Vol 45 (4) ◽  
pp. 1120-1131 ◽  
Author(s):  
Elisabeth Brouwer ◽  
Minke G. Huitema ◽  
A.H. Leontine Mulder ◽  
Peter Heeringa ◽  
Harry van Goor ◽  
...  
Keyword(s):  
Wegener’S Granulomatosis ◽  
Wegener's Granulomatosis ◽  
Neutrophil Activation

scholarly journals The Utilization of Protoporphyrin 9 in Heme Synthesis

Blood ◽  
1959 ◽  
Vol 14 (4) ◽  
pp. 476-485 ◽  
Author(s):  
MOISES GRINSTEIN ◽  
ROBIN M. BANNERMAN ◽  
CARL V. MOORE
Keyword(s):  
Cell Membrane ◽  
Human Blood ◽  
Biosynthetic Pathway ◽  
Aminolevulinic Acid ◽  
Thalassemia Major ◽  
Heme Synthesis ◽  
Equivalent Quantity ◽  
Chicken Erythrocytes

Abstract The experiments described in this communication demonstrate that C14-tagged protoporphyrin 9 can be incorporated into the heme during the biosynthesis of hemoglobin. 1. In vitro observations: (a) C14 protoporphyrin 9 was found to be incorporated into heme by hemolysates of chicken and human blood incubated at 37 C. The degree of incorporation by washed chicken erythrocytes was less, presumably because the protoporphyrin was not readily transferred across the cell membrane. Incorporation by hemolysates was inhibited completely at 1 x 10-2 M KCN at 4 C., markedly by 1 x 10-2 M KCN at 37 C. and partially by 1 x 10-3 M Pb at 37 C. (b) The degree of incorporation was reduced by the addition of an equivalent quantity of delta-aminolevulinic acid. Furthermore, the incorporation of glycine-2-C14 into heme was reduced by the addition of an equivalent quantity of protoporphyrin 9. 2. In vivo observations: Intravenously administered C14 protoporphyrin was incorporated into the circulating hemoglobin of two rabbits with a phenylhydrazine-induced hemolytic anemia. These observations provide support for the view that protoporphyrin 9 itself is a true direct precursor of hemoglobin, in the biosynthetic pathway between porphobilinogen and heme. Comparative studies of rates of incorporation of C14 protoporphyrin 9 and its precursors into heme in vitro may provide a useful tool for the study of heme synthesis in normal and pathologic conditions. For instance, it was shown that hemolysates from the blood of patients with thalassemia major, with poor iron and glycine utilization, rapidly incorporated the tagged protoporphyrin into heme.

scholarly journals Kruppel-like factor 4 regulates neutrophil activation

2017 ◽  
Vol 1 (11) ◽  
pp. 662-668 ◽  
Author(s):  
Yuyan Shen ◽  
Hong Hong ◽  
Panjamaporn Sangwung ◽  
Stephanie Lapping ◽  
Lalitha Nayak ◽  
...  
Keyword(s):  
Crucial Role ◽  
Neutrophil Function ◽  
Neutrophil Activation ◽  
Key Points

Key Points KLF4 deficiency impairs neutrophil function in vitro and in vivo. This is the first demonstration that KLF4 plays a crucial role in neutrophils.

scholarly journals Survival and recovery of human platelets stored for five days in a non- plasma medium

Blood ◽  
1986 ◽  
Vol 67 (3) ◽  
pp. 672-675 ◽  
Author(s):  
GA Adams ◽  
SD Swenson ◽  
G Rock
Keyword(s):  
Platelet Aggregation ◽  
Human Blood ◽  
Blood Platelets ◽  
Human Platelets ◽  
Plasma Medium ◽  
Release Reaction ◽  
In Vivo Recovery ◽  
Human Blood Platelets

Abstract Human blood platelets were stored for five days as concentrates in 60 mL of: (a) plasma; (b) non-plasma medium with anticoagulant; and (c) non-plasma medium without anticoagulant. All preparations were equally functional when tested for platelet aggregation and release reaction in response to single agonist or synergistic pairs of agonists in vitro. Platelets stored in non-plasma medium with anti-coagulant had lower kallikrein, fibrino(gen)peptide A, lactate, and beta-thromboglobulin than did plasma controls after five days. In vivo recovery and survival of platelets stored in non-plasma medium with anticoagulant were 51.2% +/- 4.3% and 8.7 +/- 0.3 days, respectively, which were not statistically different from plasma controls of 39.2% +/- 4.9% and 7.2 +/- 0.8 days, respectively. It is concluded that platelets can be stored for five days in a non-plasma medium and still have good in vivo recoveries and survivals.

scholarly journals Expression of LAIR-1 (CD305) on Human Blood Monocytes as a Marker of Hepatic Cirrhosis Progression

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
María Martínez-Esparza ◽  
Antonio José Ruiz-Alcaraz ◽  
Violeta Carmona-Martínez ◽  
María Dolores Fernández-Fernández ◽  
Gonzalo Antón ◽  
...  
Keyword(s):  
Liver Cirrhosis ◽  
Cell Surface ◽  
Human Blood ◽  
Total Content ◽  
Cell Surface Expression ◽  
Human Macrophage ◽  
Blood Monocytes ◽  
Cirrhotic Patients

Background and Aim. The presumed role of the inhibitory receptor LAIR-1 (CD305) in the inflammatory response suggests that it might contribute to the pathophysiology of chronic inflammatory diseases such as liver cirrhosis. We studied the LAIR-1 expression on liver macrophages and blood monocytes related to the progression of liver cirrhosis. Methods. The expression of LAIR-1 was analyzed by immunohistochemistry, flow cytometry, and Western blot. Results. We found a decreased number of macrophages expressing LAIR-1 in cirrhotic liver that could be due to a high presence of collagen, ligand of LAIR-1, in the fibrotic tissue which could downregulate its expression or interfere with the immunostaining. The expression of LAIR-1 decreased after cell differentiation, and the total content, but not the cell surface expression, increased after activation in the HL-60 human macrophage in vitro model. Blood monocytes exhibited higher LAIR-1 expression levels in cirrhotic patients, which were evident even in early clinical stages in all monocyte subsets, and greater in the “intermediate” inflammatory monocyte subpopulation. The in vitro activation of human blood monocytes did not increase its expression on the cell surface suggesting that the in vivo increase of LAIR-1 must be the result of a specific combination of stimuli present in cirrhotic patients. This represents an exclusive feature of liver cirrhosis, since blood monocytes from other chronic inflammatory pathologies showed similar or lower LAIR-1 levels compared with those of healthy controls. Conclusions. These results may indicate that monocyte LAIR-1 expression is a new biomarker to early detect liver damage caused by chronic inflammation in liver cirrhosis.

Immunological investigations of oligoethylene glycols functionalized with desaminotyrosine and desaminotyrosyltyrosine

2013 ◽  
Vol 1569 ◽  
pp. 9-14 ◽  
Author(s):  
Konstanze K. Julich-Gruner ◽  
Toralf Roch ◽  
Nan Ma ◽  
Axel T. Neffe ◽  
Andreas Lendlein
Keyword(s):  
Human Blood ◽  
High Concentration ◽  
Inflammatory Conditions ◽  
Tnf Α ◽  
High Concentrations ◽  
Pharmaceutical Agents ◽  
Immunological Evaluation ◽  
Necrosis Factor

ABSTRACTBiomaterials require thorough in vitro testing before being applied in vivo. Unwanted contaminations of biomaterials but also their intrinsic properties can cause uncontrolled immune response leading to severe consequences for the patient. Therefore, immunological evaluation of materials for biomedical applications is mandatory before entering clinical application. In order to introduce physical netpoints, the aromatic compounds desaminotyrosine (DAT) and desaminotyrosyl-tyrosine (DATT) were successfully used to functionalize linear and star-shaped oligoethylene glycol (lOEG and sOEG) as previously described. The materials showed properties of surfactants and have potential to be used for solubilization of lipophilic drugs in water. Furthermore, the materials are susceptible for H2O2 degradation as determined by MALDI-ToF MS analyses. This is important for potential in vivo applications, as macrophages can release reactive oxygen species (ROS) under inflammatory conditions. As it is known that surfactant solutions of high concentration can lead to cell lysis, the effects of OEG-DAT(T) solutions on murine RAW macrophages were investigated. Even at highest OEG-DAT(T) concentration of 1000 µg·mL-1 the viability of the RAW cells was not significantly impaired. Additionally, the polymers were incubated with whole human blood and the production of inflammatory cytokines such as the tumor necrosis factor (TNF)-α and interleukin (IL)-6 was determined. Only at high concentrations, the OEG-DAT(T) solution induced low levels of TNF-α and IL-6, indicating that a mild inflammatory reaction could be expected when such high OEG-DAT(T) concentrations are applied in vivo. Similarly, the OEG-DAT(T) solution did not induce ROS in monocytes and neutrophils after incubation with whole human blood. Conclusively, the data presented here demonstrate that OEG-DAT(T) do not lead to a substantial activation of the innate immune mechanisms and could therefore be investigated for solubilizing pharmaceutical agents.

Estimation of human blood LC50 values for use in modeling of in vitro–in vivo data of the ACuteTox project

2008 ◽  
Vol 22 (5) ◽  
pp. 1405-1411 ◽  
Author(s):  
Michael Sjöström ◽  
Ada Kolman ◽  
Cecilia Clemedson ◽  
Richard Clothier
Keyword(s):  
Human Blood
Sign in / Sign up

Export Citation Format

Share Document