Stem Cell Marker Prominin-1/AC133 Is Expressed in Duct Cells of the Adult Human Pancreas

Pancreas ◽  
2008 ◽  
Vol 36 (1) ◽  
pp. e1-e6 ◽  
Author(s):  
Jessy Lardon ◽  
Denis Corbeil ◽  
Wieland B. Huttner ◽  
Zhidong Ling ◽  
Luc Bouwens
Keyword(s):  
Stem Cell ◽  
Stem Cell Marker ◽  
Human Pancreas ◽  
Cell Marker ◽  
Adult Human ◽  
Duct Cells

scholarly journals Pluripotency-associated stem cell marker expression in proliferative cell cultures derived from adult human pancreas

2011 ◽  
Vol 211 (2) ◽  
pp. 169-176 ◽  
Author(s):  
Michael G White ◽  
Hussain R Al-Turaifi ◽  
Graham N Holliman ◽  
Ali Aldibbiat ◽  
Aiman Mahmoud ◽  
...  
Keyword(s):  
Stem Cell ◽  
Western Blot ◽  
Gradient Centrifugation ◽  
Stem Cell Marker ◽  
Stem Cell Markers ◽  
Human Pancreas ◽  
Cell Marker ◽  
Β Cells ◽  
Pancreatic Cell ◽  
Adult Human

The source of new β-cells in adult human pancreas remains incompletely elucidated with recent studies on rodents providing evidence for neogenesis from progenitor cells in addition to self-replication. The aim of this study was to investigate the expression of pluripotency-associated stem cell markers in proliferative cultures derived from adult human pancreas. Human pancreatic tissue was obtained from deceased donors following ethical approval and relative consent. Islet-enriched fraction was separated from the retrieved organ by digestion and density gradient centrifugation. Dissociated cells were seeded in adherent culture forming proliferative ‘islet survivor cells’ (ISCs). These were characterised at fifth passage by RT-PCR, immunofluorescence staining, FACS, western blot and transfection studies with an OCT4 promoter-driven reporter. Nuclear expression of the pluripotency-associated stem cell marker complex OCT4/SOX2/NANOG was confirmed in ISCs. The phenotype constituted ∼8% of the overall population. OCT4 biosynthesis was confirmed by western blot and activation of an exogenous OCT4 promoter. Co-expression of pluripotency-associated markers has been confirmed in proliferative primary cells derived from adult human pancreas. Further studies are required to elucidate whether these cells possess functional stem cell characteristics and assess potential for differentiation into pancreatic cell lineages including new β-cells.

Prominin-1/CD133, a neural and hematopoietic stem cell marker, is expressed in adult human differentiated cells and certain types of kidney cancer

2004 ◽  
Vol 319 (1) ◽  
pp. 15-26 ◽  
Author(s):  
Mareike Florek ◽  
Michael Haase ◽  
Anne-Marie Marzesco ◽  
Daniel Freund ◽  
Gerhard Ehninger ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Kidney Cancer ◽  
Stem Cell Marker ◽  
Cell Marker ◽  
Hematopoietic Stem ◽  
Adult Human ◽  
Differentiated Cells

scholarly journals Newer Insights Into Premeiotic Development of Germ Cells in Adult Human Testis Using Oct-4 as a Stem Cell Marker

2010 ◽  
Vol 58 (12) ◽  
pp. 1093-1106 ◽  
Author(s):  
Deepa Bhartiya ◽  
Sandhya Kasiviswanathan ◽  
Sreepoorna K. Unni ◽  
Prasad Pethe ◽  
Jayesh V. Dhabalia ◽  
...  
Keyword(s):  
Stem Cell ◽  
Germ Cells ◽  
Stem Cell Marker ◽  
Human Testis ◽  
Cell Marker ◽  
Adult Human

scholarly journals Oct-4 Expression in Adult Human Differentiated Cells Challenges Its Role as a Pure Stem Cell Marker

Stem Cells ◽  
2007 ◽  
Vol 25 (7) ◽  
pp. 1675-1680 ◽  
Author(s):  
Stefano Zangrossi ◽  
Mirko Marabese ◽  
Massimo Broggini ◽  
Rosaria Giordano ◽  
Marco D'Erasmo ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Marker ◽  
Cell Marker ◽  
Adult Human ◽  
Differentiated Cells

The Corneal Epithelial Stem Cell Marker - Novel Combination of High Expression of p63 and a Large N/C Ratio

2008 ◽  
Vol 1 (3) ◽  
pp. 95-105
Author(s):  
Arpitha P. ◽  
Prajna N. V. ◽  
Srinivasan M. ◽  
Muthukkaruppan V. R.
Keyword(s):  
Stem Cell ◽  
High Expression ◽  
Stem Cell Marker ◽  
Cell Marker ◽  
Corneal Epithelial ◽  
Epithelial Stem Cell ◽  
Large N
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