The Stromal Vascular Fraction Improves Maintenance of the Fat Graft Volume

2018 ◽  
Vol 81 (3) ◽  
pp. 367-371 ◽  
Author(s):  
Wei Cai ◽  
Li-dong Yu ◽  
Xiaowei Tang ◽  
Gan Shen
Keyword(s):  
Stromal Vascular Fraction ◽  
Fat Graft ◽  
Graft Volume

scholarly journals Facial Rejuvenation with Concentrated Lipograft—A 12 Month Follow-Up Study

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 594
Author(s):  
Lukas Prantl ◽  
Eva Brix ◽  
Sally Kempa ◽  
Oliver Felthaus ◽  
Andreas Eigenberger ◽  
...  
Keyword(s):  
Stromal Vascular Fraction ◽  
Facial Rejuvenation ◽  
Fat Graft ◽  
Volume Loss ◽  
Patient’S Satisfaction ◽  
Resazurin Assay ◽  
Long Term Follow Up ◽  
The Difference

Lipofilling is a popular technique to treat volume loss in aging patients. The isolated adipose tissue is composed of adipocytes and stromal vascular fraction cells, which include adipose-derived stem cells (ASC). We hypothesize that the patient’s wrinkle severity scale (WSS) and patient’s satisfaction on the global aesthetic improvement scale (GAIS) can be improved after using concentrated lipoaspirate. Fourteen patients (54 years ± 11.09 years) with volume loss in the midface area underwent waterjet-assisted liposuction (Human Med AG, Schwerin, Germany). Fat was centrifuged in an ACP Double Syringe (Arthrex GmbH, Munich, Germany) using Rotofix 32A centrifuge (Andreas Hettich, GmbH & Co.KG, Tuttlingen, Germany). Homogenization was performed using the double syringe and a 1.4 mm female–female luerlock connector. After a second centrifugation, patients received periorbital (PO) and nasolabial (NL) lipografting. ASC count was performed after enzymatical digestion. Vitality of cells was assessed using a resazurin assay. During long-term follow up (12 months, n = 10), we found a high patient’s satisfaction (GAIS 1+/−0.52) and a good improvement of the WSS during short- and long-term follow-up. The ASC count of processed lipoaspirate was 2.1-fold higher than of unprocessed lipoaspirate (p < 0.001). The difference of ASC in sedimented and simply centrifuged lipoaspirate was also significant (p < 0.05). Facial rejuvenation with concentrated fat graft offers good results concerning objective aesthetic outcome and patient’s satisfaction.

scholarly journals Engineered Fat Graft Enhanced with Adipose-Derived Stromal Vascular Fraction Cells for Regenerative Medicine: Clinical, Histological and Instrumental Evaluation in Breast Reconstruction

2019 ◽  
Vol 8 (4) ◽  
pp. 504 ◽  
Author(s):  
Pietro Gentile ◽  
Donato Casella ◽  
Enza Palma ◽  
Claudio Calabrese
Keyword(s):  
Breast Reconstruction ◽  
Preoperative Evaluation ◽  
Three Dimensional ◽  
Stromal Vascular Fraction ◽  
Fat Graft ◽  
Control Group ◽  
Study Group ◽  
Fat Tissue ◽  
Calvarial Defects

The areas in which Stromal Vascular Fraction cells (SVFs) have been used include radiotherapy based tissue damage after mastectomy, breast augmentation, calvarial defects, Crohn’s fistulas, and damaged skeletal muscle. Currently, the authors present their experience using regenerative cell therapy in breast reconstruction. The goal of this study was to evaluate the safety and efficacy of the use of Engineered Fat Graft Enhanced with Adipose-derived Stromal Vascular Fraction cells (EF-e-A) in breast reconstruction. 121 patients that were affected by the outcomes of breast oncoplastic surgery were treated with EF-e-A, comparing the results with the control group (n = 50) treated with not enhanced fat graft (EF-ne-A). The preoperative evaluation included a complete clinical examination, a photographic assessment, biopsy, magnetic resonance (MRI) of the soft tissue, and ultrasound (US). Postoperative follow-up took place at two, seven, 15, 21, 36 weeks, and then annually. In 72.8% (n = 88) of breast reconstruction treated with EF-e-A, we observed a restoration of the breast contour and an increase of 12.8 mm in the three-dimensional volume after 12 weeks, which was only observed in 27.3% (n = 33) of patients in the control group that was treated with EF-ne-A. Transplanted fat tissue reabsorption was analyzed with instrumental MRI and US. Volumetric persistence in the study group was higher (70.8%) than that in the control group (41.4%) (p < 0.0001 vs. control group). The use of EF-e-A was safe and effective in this series of treated cases.

Regenerative application of stromal vascular fraction cells enhanced fat graft maintenance: clinical assessment in face rejuvenation

2020 ◽  
Vol 20 (12) ◽  
pp. 1503-1513 ◽  
Author(s):  
Pietro Gentile ◽  
Aris Sterodimas ◽  
Claudio Calabrese ◽  
Barbara De Angelis ◽  
Angelo Trivisonno ◽  
...  
Keyword(s):  
Clinical Assessment ◽  
Stromal Vascular Fraction ◽  
Fat Graft

scholarly journals Characteristics and Potentiality of Human Adipose-Derived Stem Cells (hASCs) Obtained from Enzymatic Digestion of Fat Graft

Cells ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 282 ◽  
Author(s):  
Pietro Gentile ◽  
Maria Piccinno ◽  
Claudio Calabrese
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Ex Vivo ◽  
Stromal Vascular Fraction ◽  
Enzymatic Digestion ◽  
Histological Evaluation ◽  
Fat Graft ◽  
Adipose Derived Stem Cells ◽  
Differentiation Potential ◽  
New Therapeutic Approaches

Human adipose-derived stem cells localize in the stromal-vascular portion, and can be ex vivo isolated using a combination of washing steps and enzymatic digestion. For this study, we undertook a histological evaluation of traditional fat graft compared with fat graft enriched with stromal vascular fraction cells isolated by the Celution™ system to assess the interactions between cells and adipose tissue before the breast injection. In addition, we reported on histological analyses of biopsies derived from fat grafted (traditional or enriched with SVFs) in the breast in order to assess the quality of the adipose tissue, fibrosis and vessels. The hASCs derived from enzymatic digestion were systematically characterized for growth features, phenotype and multi-potent differentiation potential. They fulfill the definition of mesenchymal stem cells, albeit with a higher neural phenotype profile. These cells also express genes that constitute the core circuitry of self-renewal such as OCT4, SOX2, NANOG and neurogenic lineage genes such as NEUROD1, PAX6 and SOX3. Such findings support the hypothesis that hASCs may have a potential usefulness in neurodegenerative conditions. These data can be helpful for the development of new therapeutic approaches in personalized medicine to assess safety and efficacy of the breast reconstruction.

The current gold standard breast volumetry technique seems to overestimate fat graft volume retention in the breast: A validation study

2019 ◽  
Vol 72 (8) ◽  
pp. 1278-1284 ◽  
Author(s):  
Mikkel Herly ◽  
Felix Christoph Müller ◽  
Mathias Ørholt ◽  
Joachim Hansen ◽  
Sophie Sværke ◽  
...  
Keyword(s):  
Validation Study ◽  
Gold Standard ◽  
Fat Graft ◽  
Current Gold Standard ◽  
Volume Retention ◽  
Graft Volume

scholarly journals Purified Adipose-Derived Stromal Cells Provide Superior Fat Graft Retention Compared with Unenriched Stromal Vascular Fraction

2017 ◽  
Vol 139 (4) ◽  
pp. 911-914 ◽  
Author(s):  
Elizabeth R. Zielins ◽  
Elizabeth A. Brett ◽  
Charles P. Blackshear ◽  
John Flacco ◽  
Ryan C. Ransom ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Stromal Vascular Fraction ◽  
Fat Graft ◽  
Adipose Derived Stromal Cells

scholarly journals A Prospective and Controlled Clinical Trial on Stromal Vascular Fraction Enriched Fat Grafts in Secondary Breast Reconstruction

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
L. A. L. Tissiani ◽  
N. Alonso
Keyword(s):  
Breast Reconstruction ◽  
Controlled Trial ◽  
Statistical Significance ◽  
Stromal Vascular Fraction ◽  
Cell Counting ◽  
Fat Graft ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
Fat Grafts ◽  
Significant Difference

Background.Fat grafting is a tremendous tool in secondary breast reconstruction. Stromal vascular fraction (SVF) enriched fat grafts have been presenting promising results regarding volume maintenance.Methods.We developed a method that produces a superior SVF enrichment rate (2 : 1) in the operating theatre. This prospective and controlled trial analyzed quantitatively and qualitatively fat grafts with (stem cells group, SG) and without (control group, CG) SVF enrichment in secondary breast reconstruction, through MRI-based volumetry, immunophenotyping, and cell counting. Also, patient satisfaction, aesthetic outcomes, and complications were analyzed.Results.Volumetric persistence in the SG was 78,9% and 51,4% in the CG; however it did not reach statistical significant difference. CD90 was the only marker highly expressed in the SG and showed a positive correlation with volumetric persistence (r=0.651,p=0.03). Fat necrosis occurred in 4 patients in the SG and in none in the CG. Patients in the CG showed a trend to be more satisfied. Considering aesthetics, both groups presented improvements. No locoregional recurrences were observed.Conclusions.Results are encouraging despite the fact that SVF enrichment in a higher supplementation rate did not improve, with statistical significance, fat graft volumetric persistence. Enriched fat grafts have proven to be safe in a 3-year follow-up.

scholarly journals Adipose-Derived Stem Cells for Facial Rejuvenation

2022 ◽  
Vol 12 (1) ◽  
pp. 117
Author(s):  
Agnieszka Surowiecka ◽  
Jerzy Strużyna
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stromal Vascular Fraction ◽  
Extracellular Proteins ◽  
Fat Graft ◽  
Adipose Derived Stem Cells ◽  
Harvesting Technique ◽  
Fat Harvesting ◽  
Adult Mesenchymal Stem Cells ◽  
Oncologic Patients

The interest in regenerative medicine is increasing, and it is a dynamically developing branch of aesthetic surgery. Biocompatible and autologous-derived products such as platelet-rich plasma or adult mesenchymal stem cells are often used for aesthetic purposes. Their application originates from wound healing and orthopaedics. Adipose-derived stem cells are a powerful agent in skin rejuvenation. They secrete growth factors and anti-inflammatory cytokines, stimulate tissue regeneration by promoting the secretion of extracellular proteins and secrete antioxidants that neutralize free radicals. In an office procedure, without cell incubation and counting, the obtained product is stromal vascular fraction, which consists of not only stem cells but also other numerous active cells such as pericytes, preadipocytes, immune cells, and extra-cellular matrix. Adipose-derived stem cells, when injected into dermis, improved skin density and overall skin appearance, and increased skin hydration and number of capillary vessels. The main limitation of mesenchymal stem cell transfers is the survival of the graft. The final outcomes are dependent on many factors, including the age of the patient, technique of fat tissue harvesting, technique of lipoaspirate preparation, and technique of fat graft injection. It is very difficult to compare available studies because of the differences and multitude of techniques used. Fat harvesting is associated with potentially life-threatening complications, such as massive bleeding, embolism, or clots. However, most of the side effects are mild and transient: primarily hematomas, oedema, and mild pain. Mesenchymal stem cells that do not proliferate when injected into dermis promote neoangiogenesis, that is why respectful caution should be taken in the case of oncologic patients. A longer clinical observation on a higher number of participants should be performed to develop reliable indications and guidelines for transferring ADSCs.

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