scholarly journals Novel nanocomposite biomaterials with controlled copper/calcium release capability for bone tissue engineering multifunctional scaffolds

2015 ◽  
Vol 12 (110) ◽  
pp. 20150509 ◽  
Author(s):  
J. P. Cattalini ◽  
A. Hoppe ◽  
F. Pishbin ◽  
J. Roether ◽  
A. R. Boccaccini ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Calcium Release ◽  
Umbilical Vein ◽  
Burst Release ◽  
Potential Applications ◽  
Cell Property ◽  
Umbilical Vein Endothelial Cells

This work aimed to develop novel composite biomaterials for bone tissue engineering (BTE) made of bioactive glass nanoparticles (Nbg) and alginate cross-linked with Cu 2+ or Ca 2+ (AlgNbgCu, AlgNbgCa, respectively). Two-dimensional scaffolds were prepared and the nanocomposite biomaterials were characterized in terms of morphology, mechanical strength, bioactivity, biodegradability, swelling capacity, release profile of the cross-linking cations and angiogenic properties. It was found that both Cu 2+ and Ca 2+ are released in a controlled and sustained manner with no burst release observed. Finally, in vitro results indicated that the bioactive ions released from both nanocomposite biomaterials were able to stimulate the differentiation of rat bone marrow-derived mesenchymal stem cells towards the osteogenic lineage. In addition, the typical endothelial cell property of forming tubes in Matrigel was observed for human umbilical vein endothelial cells when in contact with the novel biomaterials, particularly AlgNbgCu, which indicates their angiogenic properties. Hence, novel nanocomposite biomaterials made of Nbg and alginate cross-linked with Cu 2+ or Ca 2+ were developed with potential applications for preparation of multifunctional scaffolds for BTE.

scholarly journals A review of chitosan-, alginate-, and gelatin-based biocomposites for bone tissue engineering

2018 ◽  
Vol 5 (3-4) ◽  
pp. 97-109 ◽  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Repair ◽  
Bone Diseases ◽  
Natural Polymers ◽  
Antimicrobial Substances ◽  
Potential Applications

Bone diseases and injuries have a major impact on the quality of life. Classical treatments for bone repair/regeneration/replacement have various disadvantages. Bone tissue engineering (BTE) received a great attention in the last years. Natural polymers are intensively studied in this field due to their properties (biocompatibility, biodegradability, abundance in nature, high processability). Unfortunately, their mechanical properties are poor, which is why synthetic polymers or ceramics are added in order to provide the optimal compressive, elastic or fatigue strength. Moreover, growth factors, vitamins, or antimicrobial substances are also added to enhance the cell behavior (attachment, proliferation, and differentiation). In this review, new scientific results regarding potential applications of chitosan-, alginate-, and gelatin based biocomposites in BTE will be provided, along with their in vitro and/or in vivo tests.

Novel Electrospun Bicomponent Scaffolds for Bone Tissue Engineering: Fabrication, Characterization and Sustained Release of Growth Factor

2012 ◽  
Vol 1418 ◽  
Author(s):  
Chong Wang ◽  
Min Wang ◽  
Xiao-Yan Yuan
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biological Tissues ◽  
Osteoblastic Cell ◽  
Burst Release ◽  
Electrospinning Technique ◽  
Initial Burst ◽  
Initial Burst Release

ABSTRACTElectrospinning is a versatile technique for fabricating three-dimensional (3D) nanofibrous scaffolds and the scaffolds have been found to elicit desirable cellular behavior for tissue regeneration because the nanofibrous structures mimic the nanofibrous extracellular matrix (ECM) of biological tissues. From the material point of view, the ECM of bone is a nanofibrous nanocomposite consisting of an organic matrix (mainly collagen) and inorganic bone apatite nanoparticles. Therefore, for bone tissue engineering scaffolds, it is natural to construct nanofibrous nanocomposites having a biodegradable polymer matrix and nanosized bioactive bioceramics. Our previous studies demonstrated: (1) electrospun nanocomposite fiber loaded with calcium phosphate (Ca-P) were osteoconductive and could promote osteoblastic cell proliferation and differentiation better than pure polymer fibers; (2) The controlled release of recombinant human bone morphogenetic protein (rhBMP-2) from scaffolds provided the scaffolds with desired osteoinductivity. In the current investigation, novel bicomponent scaffolds for bone tissue engineering were produced using our established dual-source dual-power electrospinning technique to achieve both osteoconductivity and osteoinductivity. In the bicomponent scaffolds, one fibrous component was electrospun Ca-P/PLGA nanocomposite fibers and the other component was emulsion electrospun PDLLA nanofibers incorporated with rhBMP-2. Through electrospinning optimization, both fibers were evenly distributed in bicomponent scaffolds. The mass ratio of rhBMP-2/PDLLA fibers to Ca-P/PLGA fibers in bicomponent scaffolds could be controlled using multiple syringes. The structure and morphology of mono- and bicomponent scaffolds were examined. The in vitro release of rhBMP-2 from mono- and bicomponent scaffolds showed different release amount but similar release profile, exhibiting an initial burst release. Blending PDLLA with polyethylene glycol (PEG) could reduce the initial burst release of rhBMP-2.

Surface modification of chitosan film via polydopamine coating to promote biomineralization in bone tissue engineering

2017 ◽  
Vol 33 (2) ◽  
pp. 134-145 ◽  
Author(s):  
Yang Liu ◽  
Zhongxun Zhang ◽  
Huilin Lv ◽  
Yong Qin ◽  
Linhong Deng
Keyword(s):  
Tissue Engineering ◽  
Surface Modification ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Photoelectron Spectroscopy ◽  
Chitosan Film ◽  
Hydrophobic Surface ◽  
Modified Chitosan ◽  
Potential Applications

Chitosan-based material has been widely used as bone substitute due to its good biocompatibility and biodegradability. However, the hydrophobic surface of chitosan film constrains the osteogenesis mineralization in the process of bone regeneration. For this reason, we develop a novel polydopamine-modified chitosan film suitable for bone tissue engineering applications by a simple and feasible route in this study. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy confirm the process of surface modification. For comparison, surface wettability, the capacity of mineralization in vitro, and biocompatibility of the chitosan film and the polydopamine-modified chitosan film were assessed. Research results indicate that the polydopamine-modified chitosan film has good hydrophilicity. It is very evident that the polydopamine treatment significantly influences the biomineralization capacity of the chitosan-based substrates, which enhance the growth rate of apatite on the modified chitosan film. Besides, MC3T3-E1 osteoblast experiments demonstrate that the cells can adhere and grow well on the polydopamine-modified chitosan film. It is anticipated that this polydopamine-modified chitosan film, which can be prepared in large quantities simply, should have potential applications in bone tissue engineering.

scholarly journals Development of an angiogenesis-promoting microvesicle-alginate-polycaprolactone composite graft for bone tissue engineering applications

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2040 ◽  
Author(s):  
Hui Xie ◽  
Zhenxing Wang ◽  
Liming Zhang ◽  
Qian Lei ◽  
Aiqi Zhao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Umbilical Vein ◽  
Composite Graft ◽  
Electron Microscopic ◽  
Engineering Applications

One of the major challenges of bone tissue engineering applications is to construct a fully vascularized implant that can adapt to hypoxic environments in vivo. The incorporation of proangiogenic factors into scaffolds is a widely accepted method of achieving this goal. Recently, the proangiogenic potential of mesenchymal stem cell-derived microvesicles (MSC-MVs) has been confirmed in several studies. In the present study, we incorporated MSC-MVs into alginate-polycaprolactone (PCL) constructs that had previously been developed for bone tissue engineering applications, with the aim of promoting angiogenesis and bone regeneration. MSC-MVs were first isolated from the supernatant of rat bone marrow-derived MSCs and characterized by scanning electron microscopic, confocal microscopic, and flow cytometric analyses. The proangiogenic potential of MSC-MVs was demonstrated by the stimulation of tube formation of human umbilical vein endothelial cellsin vitro. MSC-MVs and osteodifferentiated MSCs were then encapsulated with alginate and seeded onto porous three-dimensional printed PCL scaffolds. When combined with osteodifferentiated MSCs, the MV-alginate-PCL constructs enhanced vessel formation and tissue-engineered bone regeneration in a nude mouse subcutaneous bone formation model, as demonstrated by micro-computed tomographic, histological, and immunohistochemical analyses. This MV-alginate-PCL construct may offer a novel, proangiogenic, and cost-effective option for bone tissue engineering.

scholarly journals Dual Network Composites of Poly(vinyl alcohol)-Calcium Metaphosphate/Alginate with Osteogenic Ions for Bone Tissue Engineering in Oral and Maxillofacial Surgery

2021 ◽  
Vol 8 (8) ◽  
pp. 107
Author(s):  
Lilis Iskandar ◽  
Lucy DiSilvio ◽  
Jonathan Acheson ◽  
Sanjukta Deb
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Vinyl Alcohol ◽  
Maxillofacial Surgery ◽  
Bone Defects ◽  
Bone Tissue Regeneration ◽  
Poly Vinyl Alcohol ◽  
Oral And Maxillofacial Surgery

Despite considerable advances in biomaterials-based bone tissue engineering technologies, autografts remain the gold standard for rehabilitating critical-sized bone defects in the oral and maxillofacial (OMF) region. A majority of advanced synthetic bone substitutes (SBS’s) have not transcended the pre-clinical stage due to inferior clinical performance and translational barriers, which include low scalability, high cost, regulatory restrictions, limited advanced facilities and human resources. The aim of this study is to develop clinically viable alternatives to address the challenges of bone tissue regeneration in the OMF region by developing ‘dual network composites’ (DNC’s) of calcium metaphosphate (CMP)—poly(vinyl alcohol) (PVA)/alginate with osteogenic ions: calcium, zinc and strontium. To fabricate DNC’s, single network composites of PVA/CMP with 10% (w/v) gelatine particles as porogen were developed using two freeze–thawing cycles and subsequently interpenetrated by guluronate-dominant sodium alginate and chelated with calcium, zinc or strontium ions. Physicochemical, compressive, water uptake, thermal, morphological and in vitro biological properties of DNC’s were characterised. The results demonstrated elastic 3D porous scaffolds resembling a ‘spongy bone’ with fluid absorbing capacity, easily sculptable to fit anatomically complex bone defects, biocompatible and osteoconductive in vitro, thus yielding potentially clinically viable for SBS alternatives in OMF surgery.

scholarly journals Development of Biopolymeric Hybrid Scaffold-Based on AAc/GO/nHAp/TiO2 Nanocomposite for Bone Tissue Engineering: In-Vitro Analysis

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1319
Author(s):  
Muhammad Umar Aslam Khan ◽  
Wafa Shamsan Al-Arjan ◽  
Mona Saad Binkadem ◽  
Hassan Mehboob ◽  
Adnan Haider ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Guar Gum ◽  
Porous Scaffolds ◽  
Vitro Assay ◽  
Vitro Analysis ◽  
Nanocomposite Scaffolds

Bone tissue engineering is an advanced field for treatment of fractured bones to restore/regulate biological functions. Biopolymeric/bioceramic-based hybrid nanocomposite scaffolds are potential biomaterials for bone tissue because of biodegradable and biocompatible characteristics. We report synthesis of nanocomposite based on acrylic acid (AAc)/guar gum (GG), nano-hydroxyapatite (HAp NPs), titanium nanoparticles (TiO2 NPs), and optimum graphene oxide (GO) amount via free radical polymerization method. Porous scaffolds were fabricated through freeze-drying technique and coated with silver sulphadiazine. Different techniques were used to investigate functional group, crystal structural properties, morphology/elemental properties, porosity, and mechanical properties of fabricated scaffolds. Results show that increasing amount of TiO2 in combination with optimized GO has improved physicochemical and microstructural properties, mechanical properties (compressive strength (2.96 to 13.31 MPa) and Young’s modulus (39.56 to 300.81 MPa)), and porous properties (pore size (256.11 to 107.42 μm) and porosity (79.97 to 44.32%)). After 150 min, silver sulfadiazine release was found to be ~94.1%. In vitro assay of scaffolds also exhibited promising results against mouse pre-osteoblast (MC3T3-E1) cell lines. Hence, these fabricated scaffolds would be potential biomaterials for bone tissue engineering in biomedical engineering.

scholarly journals Three-dimensionally printed polycaprolactone/multicomponent bioactive glass scaffolds for potential application in bone tissue engineering

2020 ◽  
Vol 6 (1) ◽  
pp. 57-69
Author(s):  
Amirhosein Fathi ◽  
Farzad Kermani ◽  
Aliasghar Behnamghader ◽  
Sara Banijamali ◽  
Masoud Mozafari ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Layer By Layer ◽  
Composite Scaffolds ◽  
3D Printed ◽  
Co Doped

AbstractOver the last years, three-dimensional (3D) printing has been successfully applied to produce suitable substitutes for treating bone defects. In this work, 3D printed composite scaffolds of polycaprolactone (PCL) and strontium (Sr)- and cobalt (Co)-doped multi-component melt-derived bioactive glasses (BGs) were prepared for bone tissue engineering strategies. For this purpose, 30% of as-prepared BG particles (size <38 μm) were incorporated into PCL, and then the obtained composite mix was introduced into a 3D printing machine to fabricate layer-by-layer porous structures with the size of 12 × 12 × 2 mm3.The scaffolds were fully characterized through a series of physico-chemical and biological assays. Adding the BGs to PCL led to an improvement in the compressive strength of the fabricated scaffolds and increased their hydrophilicity. Furthermore, the PCL/BG scaffolds showed apatite-forming ability (i.e., bioactivity behavior) after being immersed in simulated body fluid (SBF). The in vitro cellular examinations revealed the cytocompatibility of the scaffolds and confirmed them as suitable substrates for the adhesion and proliferation of MG-63 osteosarcoma cells. In conclusion, 3D printed composite scaffolds made of PCL and Sr- and Co-doped BGs might be potentially-beneficial bone replacements, and the achieved results motivate further research on these materials.

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