scholarly journals Identification of 16,25- O -diacetyl-cucurbitane F and 25- O -acetyl-23,24-dihydrocucurbitacin F as novel anti-cancer chemicals

2018 ◽  
Vol 5 (8) ◽  
pp. 180723 ◽  
Author(s):  
Wenxue Wang ◽  
Haoran Yang ◽  
Ying Li ◽  
Zhongfei Zheng ◽  
Yongjun Liu ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Therapeutic Option ◽  
Human Colon ◽  
Phase Cell ◽  
Resonance Spectroscopy ◽  
Ionization Mass ◽  
Human Colon Cancer ◽  
Cycle Arrest ◽  
Ht29 Cells ◽  
M Phase

Seven new cucurbitane glucosides, hemslepensides J-P ( 1 – 7 ), and two known compounds, 16,25- O -diacetyl-cucurbitane F ( 8 ) and 25- O -acetyl-23,24-dihydrocucurbitacin F ( 9 ), were isolated from the tubers of Hemsleya pengxianensis var. jinfushanensis . The structures of 1 – 7 were elucidated using infrared absorption spectroscopy, nuclear magnetic resonance spectroscopy and high-resolution electrospray ionization mass spectrometry. The treatment of HT29 cells, human colon cancer cells, with compounds 8 and 9 inhibited cell proliferation. Further study demonstrated that compounds 8 and 9 induced F-actin aggregation, G 2 /M phase cell cycle arrest and cell apoptosis in HT29 cells. In summary, the present study enriched the chemical composition research of H. pengxianensis , and suggested that the compounds 8 / 9 treatment may be a potentially useful therapeutic option for colon cancer.

scholarly journals Inhibition of Anchorage-Independent Proliferation and G0/G1 Cell-Cycle Regulation in Human Colorectal Carcinoma Cells by 4,7-Dimethoxy-5-Methyl-l,3-Benzodioxole Isolated from the Fruiting Body ofAntrodia camphorate

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Hsiu-Man Lien ◽  
Hsiao-Wei Lin ◽  
Ying-Jan Wang ◽  
Li-Ching Chen ◽  
Ding-Yah Yang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Colon Cancer ◽  
Cell Cycle Arrest ◽  
Colony Formation ◽  
Human Colon ◽  
Soft Agar ◽  
Phase Cell ◽  
M Cell ◽  
Human Colon Cancer ◽  
Cycle Arrest

In this study, 4,7-dimethoxy-5-methyl-l,3-benzodioxole (SY-1) was isolated from three different sources of dried fruiting bodies ofAntrodia camphorate(AC).ACis a medicinal mushroom that grows on the inner heartwood wall ofCinnamomum kanehirai Hay(Lauraceae), an endemic species that is used in Chinese medicine for its anti-tumor and immunomodulatory properties. In this study, we demonstrated that SY-1 profoundly decreased the proliferation of human colon cancer cells (COLO 205) through G0/G1 cell-cycle arrest (50–150 μM) and induction of apoptosis (>150 μM). Cell-cycle arrest induced by SY-1 was associated with a significant increase in levels of p53, p21/Cip1 and p27/Kip1, and a decrease in cyclins D1, D3 and A. In contrast, SY-1 treatment did not induce significant changes in G0/G1 phase cell-cycle regulatory proteins in normal human colonic epithelial cells (FHC). The cells were cultured in soft agar to evaluate anchorage-independent colony formation, and we found that the number of transformed colonies was significantly reduced in the SY-1-treated COLO 205 cells. These findings demonstrate for the first time that SY-1 inhibits human colon cancer cell proliferation through inhibition of cell growth and anchorage-independent colony formation in soft agar. However, the detailed mechanisms of these processes remain unclear and will require further investigation.

Sign in / Sign up

Export Citation Format

Share Document