Growth-promoting effects of insulin and growth hormone in hypophysectomized and diethylstilboestrol-treated rats, and in rats fed 2-4-dinitrophenol

1956 ◽  
Vol 145 (919) ◽  
pp. 259-270 ◽  
Keyword(s):  
Growth Hormone ◽  
High Energy ◽  
Nitrogen Excretion ◽  
Promoting Effect ◽  
Protamine Zinc Insulin ◽  
Normal Limits ◽  
Pancreatic Insulin ◽  
Liver And Pancreas ◽  
Hypophysectomized Rats ◽  
Growth Promoting

Protamine zinc insulin has no growth-promoting effect in rats whose growth has been inhibited by diethylstilboestrol. Hypophyseal growth hormone stimulates growth in diethylstilboestrol-treated rats, and treatment with growth hormone and protamine zinc insulin results in an enhanced rate of growth. Protamine zinc insulin has growth-promoting properties in hypophysectomized rats, and cortisone acetate inhibits growth in insulin-treated hypophysectomized rats. 2-4-Dinitrophenol prevents growth and increases nitrogen excretion in young rats. Combined treatm ent with insulin, growth hormone and glucose induces growth and brings about positive nitrogen balance in dinitrophenol-poisoned rats. Adrenal, testis, liver and pancreas weights are normal in dinitrophenol-poisoned rats. The amount of extractable pancreatic insulin is also within normal limits. It is suggested that the functions of the hypophysis and pancreas are not seriously impaired, and that treatment with growth hormone and insulin promotes esterification of inorganic phosphate to high-energy phosphate compounds in the dinitrophenol-poisoned rats.

THE BIOASSAY OF GROWTH HORMONE IN SNELL'S DWARF MICE: EFFECTS OF THYROXINE AND PROLACTIN ON THE DOSE—RESPONSE CURVE

1973 ◽  
Vol 56 (2) ◽  
pp. 235-243 ◽  
Author(s):  
M. WALLIS ◽  
JENNIFER A. DEW
Keyword(s):  
Growth Hormone ◽  
Response Curve ◽  
Growth Response ◽  
Growth Promotion ◽  
Promoting Effect ◽  
Pituitary Growth Hormone ◽  
Growth Promoting ◽  
Dependent Growth ◽  
Dwarf Mice ◽  
Dose Dependent

SUMMARY Pituitary growth hormone has a dose-dependent growth promoting effect in pituitary dwarf mice (Snell's strain), and this effect can be used as the basis of a bioassay for the hormone. Prolactin and thyroxine also promote growth in these animals, and the effects of these hormones in combination with growth hormone were studied, in order to see whether their presence might enhance the precision or sensitivity of the growth hormone assay. When prolactin and/or thyroxine were administered with growth hormone, the growth response observed was no greater than the sum of the effects of the hormones given separately; in some cases it was less. Neither prolactin nor thyroxine increase the sensitivity or precision of the growth hormone bioassay. The implications of these results for theories about the mechanisms of growth promotion by these hormones are considered.

SOMATOSTATIN IMMUNIZATION AND GROWTH OF LAMBS

1986 ◽  
Vol 66 (1) ◽  
pp. 77-83 ◽  
Author(s):  
B. LAARVELD ◽  
R. K. CHAPLIN ◽  
D. E. KERR
Keyword(s):  
Growth Hormone ◽  
Glucose Tolerance ◽  
Key Words ◽  
Standard Error ◽  
Average Daily Gain ◽  
Promoting Effect ◽  
Growth Promoting ◽  
Daily Gain

Twenty cross-bred lambs were used in a replicated design to measure the growth-promoting effect of immunization against somatostatin. The lambs were immunized against human alpha-globulin (control) or against somatostatin coupled to human alpha-globulin in a 1:3.75 ratio (wt/wt) (SI). The first immunization occurred when the lambs were 27 d old, followed by three additional immunizations at 2-wk intervals. Growth was monitored from first immunization until the lambs were 103 d old. At the age of 103 d the SI lambs were heavier than the control lambs (33.2 ± 1.1 (mean ± standard error) vs. 30.1 ± 1.4 kg, P < 0.05). The average daily gain after first immunization of the SI lambs was 17% greater than that of the control lambs (310 ± 10 vs. 265 ± 10 g day−1, P < 0.05). Growth hormone and insulin secretory responses to glucose and arginine, glucose tolerance and somatomedin levels in plasma were not different between groups. Somatostatin immunization increased growth in lambs, but the mechanisms through which this occurs are not clear. Key words: Somatostatin, immunization, growth, lambs

scholarly journals Recombinant human growth hormone enhances tibial growth in peripubertal female rats but not in males

2000 ◽  
pp. 517-523 ◽  
Author(s):  
MA Rol De Lama ◽  
A Perez-Romero ◽  
JA Tresguerres ◽  
M Hermanussen ◽  
C Ariznavarreta
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Adult Male ◽  
Female Rats ◽  
Male Rats ◽  
Invasive Technique ◽  
Promoting Effect ◽  
Gh Treatment ◽  
Catch Up ◽  
Growth Promoting

OBJECTIVE: A novel non-invasive technique termed microknemometry, which allows daily leg length measurement, was used to investigate the growth promoting effect of growth hormone (GH) on peripubertal rats. We compared the effect of different patterns of recombinant human (rh) GH administration to peripubertal male rats with the effect produced by two daily administrations of the same amount of rhGH to peripubertal female rats or adult male rats. Another group of peripubertal male rats was also submitted to a 3-day period of starvation, in order to study catch-up growth during refeeding and to determine whether this process could be stimulated by exogenous GH administration. RESULTS: GH treatment was unable to stimulate tibial growth or weight gain in peripubertal males, whereas a clear growth promoting effect was observed in female rats and also in adult male rats. Starvation caused a dramatic body weight loss, and a reduction in tibial growth rate. Peripubertal male rats gained body weight faster than unstarved animals during refeeding, although recovery was not complete after nine days. Tibial growth, however, was resumed at the same speed as in normally fed males. This means that no catch-up effect was observed after refeeding in animals either with or without GH treatment. CONCLUSIONS: During peripuberty, normal male rats grow at a maximal speed that cannot be further increased by exogenous GH treatment, whereas age-matched female rats or older males grow at a slower rate than peripubertal males. Thus, exogenous rhGH administration is capable of enhancing growth velocity.

Growth-Promoting Effect of Growth Hormone and Low Dose Ethinyl Estradiol in Girls with Turner's Syndrome*

1990 ◽  
Vol 70 (1) ◽  
pp. 122-126 ◽  
Author(s):  
MAGDA VANDERSCHUEREN-LODEWEYCKX ◽  
GUY MASSA ◽  
MARC MAES ◽  
MARGARETHA CRAEN ◽  
GUY VAN VLIET ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Low Dose ◽  
Ethinyl Estradiol ◽  
Turner's Syndrome ◽  
Turner’S Syndrome ◽  
Promoting Effect ◽  
Growth Promoting

Potentiation of the growth-promoting activity of porcine growth hormone (pGH) with an antibody generated in rabbits to the peptide sequence pGH110–118

1990 ◽  
Vol 127 (3) ◽  
pp. 481-485 ◽  
Author(s):  
B. S. Wang ◽  
E. Szewczyk ◽  
H.-M. Shieh ◽  
I. C. Hart
Keyword(s):  
Amino Acids ◽  
Growth Hormone ◽  
Growth Rate ◽  
The Other ◽  
Peptide Sequence ◽  
Antigenic Epitope ◽  
Peptide Antigens ◽  
Epitope Specificity ◽  
Hypophysectomized Rats ◽  
Growth Promoting

ABSTRACT An effort was made to identify the antigenic epitope(s) of porcine GH (pGH) capable of inducing antibodies which would enhance the growth-promoting activity of the hormone. Several peptide sequences of the pGH molecule were synthesized and the antibodies to these peptides were generated in rabbits. The majority of these antibodies were found to be immunoreactive with both intact pGH and their respective peptide antigens. The biological activity of the antibodies was tested in growth-retarded hypophysectomized rats. Daily injections with pGH alone significantly increased their growth rate and treatment with a combination of pGH and antibody specific to the peptide sequence 110–118 further heightened the somatogenic effect. Administration of this antibody alone did not enhance the growth rate of hypophysectomized rats, neither did antibodies raised to intact pGH or the other peptide sequences. The present findings suggest that antibody with a particular pGH epitope specificity is able to increase the somatogenic activity of pGH and that the peptide corresponding to pGH amino acids 110–118 may prove useful in the development of growth promoting vaccines. Journal of Endocrinology (1990) 127, 481–485

scholarly journals Factors Attenuating Growth Promoting Effect of Growth Hormone Therapy for Turner's Syndrome.

1988 ◽  
Vol 35 (5) ◽  
pp. 725-731 ◽  
Author(s):  
TAMOTU SATO ◽  
CHIAKI MIYAMORI ◽  
SOHEI KAJIWARA ◽  
KAZUHIKO MIYAGAWA ◽  
MAKOTO SHIMIZU
Keyword(s):  
Growth Hormone ◽  
Hormone Therapy ◽  
Growth Hormone Therapy ◽  
Turner's Syndrome ◽  
Turner’S Syndrome ◽  
Promoting Effect ◽  
Growth Promoting
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