Electron microscopic studies of macromolecules without appositional contrast

1971 ◽  
Vol 261 (837) ◽  
pp. 143-149 ◽  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Carbon Support ◽  
Biological Macromolecules ◽  
Large Area ◽  
Electron Microscopic ◽  
Conventional Transmission Electron Microscopy ◽  
Transmission Electron ◽  
Protein Monolayers ◽  
Microscopic Studies

Dedicated to the memory of Nicole Granboulan In pursuing electron microscopic studies of individual biomacromolecules without contrast-enhancing stains, conventional transmission electron microscopy was improved by the use of thin, large area carbon support films. Contrast of the bright-held appearance of the unstained macromolecules and complexes was strongly enhanced in a dark held obtained by insertion of a dark-held objective aperture or by tilting the condenser system. The results are exemplified by various biological macromolecules such as DNA-protein monolayers or ferritin molecules.

scholarly journals Transmission electron microscopy enables the reconstruction of the catenane and ring forms of CS2 hydrolase

2014 ◽  
Vol 50 (71) ◽  
pp. 10281-10283 ◽  
Author(s):  
Joseph Che-Yen Wang ◽  
Adam Zlotnick ◽  
Jasmin Mecinović
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Direct Evidence ◽  
Protein Assemblies ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Transmission Electron Microscopic ◽  
Microscopic Studies ◽  
Good Agreement

Transmission electron microscopic studies on CS2 hydrolase provide direct evidence for the existence of the hexadecameric catenane and octameric ring topologies. Reconstructions of both protein assemblies are in good agreement with crystallographic analyses.

The blister of porphyria cutanea tarda: A Scanning and Transmission Electron Microscopic study

1986 ◽  
Vol 44 ◽  
pp. 334-335
Author(s):  
Veronika Burmeister ◽  
R. Swaminathan
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Basement Membrane ◽  
Middle Age ◽  
Porphyria Cutanea Tarda ◽  
Minor Trauma ◽  
Electron Microscopic ◽  
Transmission Electron Microscopic Study ◽  
Transmission Electron ◽  
Transmission Electron Microscopic

Porphyria cutanea tarda (PCT) is a disorder of porphyrin metabolism which occurs most often during middle age. The disease is characterized by excessive production of uroporphyrin which causes photosensitivity and skin eruptions on hands and arms, due to minor trauma and exposure to sunlight. The pathology of the blister is well known, being subepidermal with epidermodermal separation, it is not always absolutely clear, whether the basal lamina is attached to the epidermis or the dermis. The purpose of our investigation was to study the attachment of the basement membrane in the blister by comparing scanning with transmission electron microscopy.

Light and electron microscopic investigations in the Dictyonemataceae (Basidiolichens)

1977 ◽  
Vol 55 (20) ◽  
pp. 2565-2573 ◽  
Author(s):  
Robert D. Slocum ◽  
Gary L. Floyd
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cellular Level ◽  
Fungal Symbiont ◽  
Electron Microscopic ◽  
Basidiomycetous Fungus ◽  
Transmission Electron ◽  
Fungal Symbiosis ◽  
Algal Host ◽  
Microscopic Investigations

The nature of the association between the basidiomycetous mycobiont and the blue-green phycobiont in two species of the tropical basidiolichen Dictyonema was investigated using Nomarski light optics and scanning and transmission electron microscopy. Although members of this family may exhibit either a homoiomerous or heteromerous type of thallus organization, the fungus–alga relationship at the cellular level is remarkably consistent. Scytonema filaments are intimately associated with appressorial hyphae of the mycobiont and with extensive intracellular hyphae, which appear to be unrelated to the basidiomycetous fungal symbiont. This is the first report of a lichen displaying an apparent dual fungal symbiosis with the algal host. Association with the intracellular fungus produces no discernible damage to the phycobiont and apparently does not interfere with the symbiosis involving the basidiomycetous fungus.

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