scholarly journals Mannan-protein Location and Biosynthesis in Plasma Membranes from the Yeast Form of Candida albicans

1977 ◽  
Vol 103 (1) ◽  
pp. 51-59 ◽  
Author(s):  
M. S. Marriott
Keyword(s):  
Candida Albicans ◽  
Plasma Membranes ◽  
Yeast Form ◽  
Protein Location

ENTERIC CANDIDIASIS

PEDIATRICS ◽  
1962 ◽  
Vol 30 (1) ◽  
pp. 71-85
Author(s):  
Philip J. Kozinn ◽  
Claire L. Taschdjian
Keyword(s):  
Candida Albicans ◽  
A Priori ◽  
Yeast Form ◽  
Direct Microscopic Examination ◽  
Comparative Response ◽  
Clinical Clue ◽  
Oral Thrush ◽  
The Difference ◽  
Cutaneous Candidiasis ◽  
Stool Cultures

Direct microscopic examination of fecal smears permits differentiation between the saprophytic and the pathogenic phase of intestinal Candida albicans. The pathogenic phase is characterized by the presence of mycelia (M) in direct smears. When C. albicans is present saprophytically, direct fecal smears are negative or show the yeast form (Y) only. MY-positive fecal smears were found with nine times higher frequency in abnormal than in normal-appearing stools of infants with oral thrush who yielded C. albicans in stool cultures. Candidal enteritis was diagnosed on the basis of MY-positive fecal smears in 27 patients, and was ruled out on the basis of MY-negative fecal smears in six patients who yielded the organism in cultures only. The validity of the a priori diagnosis was tested by the comparative response of the MY-positive and the MY-negative patients to specific anticandidal therapy (nystatin) given by mouth. Of the patients, 88% responded clinically and mycologically as predicted on the basis of direct mycological stool examinations. Of the MY-positive patients, 80% responded to nystatin within an average of 3 days' therapy. All six MY-negative patients failed to respond within an average of 12 days' therapy. The difference in response was found to be statistically significant. Oral and/or cutaneous candidiasis was present in 85% of the MY- positive patients. The presence of oral and/or cutaneous candidiasis in a patient with diarrhea furnishes a clinical clue to probable candidal etiology of the enteritis.

scholarly journals Simple Carbohydrate Derivatives Diminish the Formation of Biofilm of the Pathogenic Yeast Candida albicans

Antibiotics ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 10 ◽  
Author(s):  
Olena P. Ishchuk ◽  
Olov Sterner ◽  
Ulf Ellervik ◽  
Sophie Manner
Keyword(s):  
Candida Albicans ◽  
Fungal Pathogen ◽  
Biofilm Formation ◽  
Untreated Control ◽  
Morphological Transition ◽  
Pathogenic Yeast ◽  
Yeast Form ◽  
Human Fungal Pathogen ◽  
Morphological Transitions ◽  
Simple Carbohydrate

The opportunistic human fungal pathogen Candida albicans relies on cell morphological transitions to develop biofilm and invade the host. In the current study, we developed new regulatory molecules, which inhibit the morphological transition of C. albicans from yeast-form cells to cells forming hyphae. These compounds, benzyl α-l-fucopyranoside and benzyl β-d-xylopyranoside, inhibit the hyphae formation and adhesion of C. albicans to a polystyrene surface, resulting in a reduced biofilm formation. The addition of cAMP to cells treated with α-l-fucopyranoside restored the yeast-hyphae switch and the biofilm level to that of the untreated control. In the β-d-xylopyranoside treated cells, the biofilm level was only partially restored by the addition of cAMP, and these cells remained mainly as yeast-form cells.

Roles of Ca2+ in hyphal and yeast-form growth in Candida albicans. Growth regulation by altered extracellular and intracellular free Ca2+ concentrations

Mycoscience ◽  
1997 ◽  
Vol 38 (2) ◽  
pp. 215-225
Author(s):  
Shuichi Sakaguchi ◽  
Kyoko Shibuya ◽  
Hidetoshi lida ◽  
Yasuhiro Anraku ◽  
Takahito Suzuki
Keyword(s):  
Candida Albicans ◽  
Growth Regulation ◽  
Yeast Form

scholarly journals Undecylenic Acid Inhibits Morphogenesis ofCandida albicans

2000 ◽  
Vol 44 (10) ◽  
pp. 2873-2875 ◽  
Author(s):  
Nealoo McLain ◽  
Rhoda Ascanio ◽  
Carol Baker ◽  
Robert A. Strohaver ◽  
Joseph W. Dolan
Keyword(s):  
Candida Albicans ◽  
Undecylenic Acid ◽  
Denture Stomatitis ◽  
Yeast Form ◽  
Ofcandida Albicans

ABSTRACT Resilient liners are frequently used to treat denture stomatitis, a condition often associated with Candida albicansinfections. Of 10 liners tested, 2 were found to inhibit the switch from the yeast form to hyphae and a third was found to stimulate this switch. The inhibitor was determined to be undecylenic acid.

Adhesin - receptor interactions in the attachment ofCandida albicansto host epithelial cells

1995 ◽  
Vol 73 (S1) ◽  
pp. 1147-1153 ◽  
Author(s):  
L. Julia Douglas
Keyword(s):  
Candida Albicans ◽  
Epithelial Cells ◽  
Blood Group Antigen ◽  
Receptor Interaction ◽  
Environmental Signals ◽  
Yeast Form ◽  
Adhesion Receptor ◽  
Receptor Interactions ◽  
Fimbrial Adhesin ◽  
Protein Portion

The ability of Candida albicans to adhere to a variety of host surfaces is thought to be an important factor in the pathogenesis of candidosis. Adhesion of the yeast form of the fungus to epithelial cells can involve several kinds of adhesion – receptor interaction. Yeast adhesins are typically mannoproteins associated with fibrils or fimbriae on the fungal surface. Lectinlike interactions have been identified between the protein portion of two mannoprotein adhesins and glycosides containing L-fucose or N-acetylglucosamine. The fucoside-binding adhesin has been purified and shown to have an affinity for glycosphingolipid receptors carrying the H blood-group antigen. A fimbrial adhesin has also been described that binds to gangliosides containing a βGalNAc(1–4)βGal disaccharide sequence. Other mannoprotein adhesins proposed recently include the factor 6 epitope present on serotype A strains of C. albicans and an integrin analogue. Adhesin expression appears to be regulated by a number of environmental signals, including osmolarity and the availability of iron and sugars. Additional adhesion-dependent signals might trigger further responses such as the initiation of morphogenesis. Key words: Candida albicans, yeast adhesion, epithelial cell adhesion.

The Candida albicans cell wall protein Rhd3/Pga29 is abundant in the yeast form and contributes to virulence

Yeast ◽  
2010 ◽  
Vol 27 (8) ◽  
pp. 611-624 ◽  
Author(s):  
Albert D. de Boer ◽  
Piet W. J. de Groot ◽  
Günther Weindl ◽  
Martin Schaller ◽  
Dietmar Riedel ◽  
...  
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Cell Wall Protein ◽  
Yeast Form

scholarly journals The Effect of Oral Probiotic Streptococcus Salivarius K12 on Candida Albicans Biofilm Formation

2020 ◽  
Vol 18 (2) ◽  
Author(s):  
Munirah Mokhtar ◽  
Alia Risma Rismayuddin ◽  
Ridhwan Abdul Wahab ◽  
Muhamad Ashraf Rostam ◽  
Mohd Hamzah Mohd Nasir ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Oral Cancer ◽  
Biofilm Formation ◽  
Streptococcus Salivarius ◽  
Yeast Form ◽  
Cell Numbers ◽  
Crystal Violet Assay ◽  
Common Cancer ◽  
The Media ◽  
Candida Albicans Infection

Introduction:  Oral cancer is the sixth most common cancer worldwide with  Candida albicans  infection being one of the aetiological factors for the disease. Meanwhile,  Streptococcus salivarius  K12 is an oral probiotic that is beneficial to the oral cavity. The objective of the present study is to determine the effect of  S. salivarius  K12 on  C. albicans  biofilm-forming ability with the hypothesis that  S. salivarius  K12 inhibits biofilm formation of  C. albicans  Materials and method: To assess the effect of  S. salivarius  K12 on  C. albicans  biofilm formation,  S. salivarius  K12, lab strain  C. albicans  MYA-4901 and clinical isolates from oral cancer, ALC2 and ALC3 were grown in both nutrient broth (NB) and RPMI. In a mono-species biofilm, 105 of  C. albicans  cells and 106 of  S. salivarius  K12 cells were grown separately in a 96-well plate. In contrast, both microorganisms were combined for polymicrobial biofilms with similar cell numbers as in mono-species. The biofilms were incubated for 72 hours at 37°C and the media were replenished every 24 hours. Finally, the crystal violet assay was conducted, and the optical density was measured at OD620nm.  Results: Polymicrobial biofilms of  C. albicans  (MYA-4901 and ALC3) with  S. salivarius K12 when grown in NB, exhibited a decrease by 64.5 ± 25.8% and 83.7 ± 5.4%, respectively when compared to the expected biofilms which were predominated by yeast form.  Furthermore, polymicrobial biofilms of  C. albicans  (ALC2 and ALC3) with  S. salivarius  K12 showed a decrease by 62.5 ± 25.6% and 55.9 ± 17.1 %, respectively when compared to the expected biofilms when grown in RPMI that were predominated by hyphal form.  Conclusion:  S. salivarius  K12 inhibited polymicrobial biofilms formation of  C. albicans  yeast and hyphal forms, thus supported the hypothesis that  S. salivarius  K12 inhibits biofilm formation of  C. albicans.

scholarly journals Candida Albicans Activated Splenocytes Promote Strong Immune Responses in a Murine Model of Breast Cancer

2021 ◽  
Author(s):  
Siavash Mashhouri ◽  
Erfan Yarahmadi ◽  
Seyyed Meysam Abtahi Froushani
Keyword(s):  
Breast Cancer ◽  
Candida Albicans ◽  
Immune Responses ◽  
Survival Curve ◽  
Tumor Development ◽  
Yeast Form ◽  
Tumor Bearing ◽  
4T1 Cells ◽  
Breast Cancer Growth

Background: The potential of Candida albicans to modulate antigen-presenting cells maturation has been documented in past studies. Dendritic cells are critical modulators in the orchestration of adaptive immune responses alongside myeloid subtypes, which play an important role in the presentation of antigens to T cells. The aim of this study was to evaluate the efficacy of splenocytes activated with the extract of heated 4T1 cells and the yeast form of C. albicans against breast cancer growth in vivo. Methods: 4T1 cells were subcutaneously injected into the left flanks of female BALB/c mice (n=40). At a time when palpable tumors had developed, experimental groups were immunized twice at one-week interim with either activated splenocytes with the extract of heated 4T1 or the killed preparation of yeast form of C. albicans or a combination of the two-One week after the second injection, one-half of animals (n=20) were euthanized to investigate the immune response profile. Results: Administration of activated splenocytes with the combination protocol caused a favorable survival curve and slower rates of tumor development compared to other tumor-bearing mice. Moreover, combination therapy significantly increased the secretion of IFN-γ, respiratory burst and nitric oxide production and conversely diminished the secretion of IL-4, IL-10 and TGF-β in the splenocyte population. Conclusion: Since the murine 4T1 cell line is similar to the final stage of human breast carcinoma, we postulate that activated splenocytes with the extract of heated 4T1 cells and yeast form of C. albicans can reduce tumor development in tumor-bearing mice.

scholarly journals Intravital Imaging of Candida albicans Identifies Differential In Vitro and In Vivo Filamentation Phenotypes for Transcription Factor Deletion Mutants

mSphere ◽  
2021 ◽  
Author(s):  
Rohan S. Wakade ◽  
Manning Huang ◽  
Aaron P. Mitchell ◽  
Melanie Wellington ◽  
Damian J. Krysan
Keyword(s):  
Candida Albicans ◽  
Fungal Infections ◽  
Deletion Mutants ◽  
Filamentous Form ◽  
Content Type ◽  
Yeast Form ◽  
Common Causes ◽  
Do So

Candida albicans is one of the most common causes of fungal infections in humans. C. albicans undergoes a transition from a round yeast form to a filamentous form during infection, which is critical for its ability to cause disease. Although this transition has been studied in the laboratory for years, methods to do so in an animal model of infection have been limited.

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