scholarly journals Stappia indica sp. nov., isolated from deep seawater of the Indian Ocean

2010 ◽  
Vol 60 (4) ◽  
pp. 733-736 ◽  
Author(s):  
Qiliang Lai ◽  
Nan Qiao ◽  
Changliang Wu ◽  
Fengqin Sun ◽  
Jun Yuan ◽  
...  
Keyword(s):  
Indian Ocean ◽  
Dna Hybridization ◽  
Sequence Similarity ◽  
Tween 80 ◽  
Rrna Gene ◽  
Chromosomal Dna ◽  
Deep Seawater ◽  
Phenotypic Data ◽  
Polycyclic Aromatic ◽  
The Indian Ocean

A taxonomic study was carried out on strain B106T, which was isolated from a polycyclic aromatic hydrocarbon-degrading consortium, enriched with deep seawater from the Indian Ocean. The isolate was Gram-negative, oxidase- and catalase-positive, rod-shaped and motile by means of one polar flagellum. Growth was observed at salinities of 0.5–11 % and at temperatures of 4–42 °C, and the strain was capable of nitrate reduction, but was unable to degrade Tween 80 or gelatin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain B106T belonged to the genus Stappia, with a highest sequence similarity of 97.7 % to Stappia stellulata IAM 12621T; similarity to other strains was below 95.2 %. DNA–DNA hybridization between strain B106T and S. stellulata IAM 12621T was 43 %. The major fatty acids were C16 : 0 (6.10 %), C18 : 1 ω7c (62.58 %), C18 : 0 (5.17 %), C18 : 1 ω7c 11-methyl (14.48 %) and C19 : 0 ω8c cyclo (4.70 %). The G+C content of the chromosomal DNA was 65.9 mol%. The combined genotypic and phenotypic data showed that strain B106T represents a novel species of the genus Stappia, for which the name Stappia indica sp. nov. is proposed, with the type strain B106T (=PR56-8T=CCTCC AB 208228T=LMG 24625T=MCCC 1A01226T).

scholarly journals Parvibaculum indicum sp. nov., isolated from deep-sea water

2011 ◽  
Vol 61 (2) ◽  
pp. 271-274 ◽  
Author(s):  
Qiliang Lai ◽  
Liping Wang ◽  
Yuhui Liu ◽  
Jun Yuan ◽  
Fengqin Sun ◽  
...  
Keyword(s):  
Deep Sea ◽  
Sea Water ◽  
Sequence Similarity ◽  
Tween 80 ◽  
Rrna Gene ◽  
Chromosomal Dna ◽  
Phenotypic Data ◽  
Respiratory Quinone ◽  
Deep Sea Water ◽  
Polycyclic Aromatic

A taxonomic study was carried out on strain P31T, which was isolated from a polycyclic aromatic hydrocarbon (PAH)-degrading consortium enriched with deep-sea water of the Indian Ocean. The isolate was Gram-reaction-negative, rod-shaped, motile by means of a polar flagellum and incapable of reducing nitrate to nitrite. Growth was observed at 0.5–8 % NaCl and at 10–41 °C. Strain P31T was unable to degrade Tween 80 or gelatin. The major respiratory quinone was ubiquinone 11 (Q-11). The dominant fatty acids were C18 : 1 ω7c (39.79 %), 11-methyl C18 : 1 ω7c (17.84 %), C19 : 0 cyclo ω8c (12.05 %) and C18 : 0 (6.09 %). The G+C content of the chromosomal DNA was 62.1 mol%. A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strain P31T and Parvibaculum lavamentivorans DS-1T formed a distinct lineage in the family Phyllobacteriaceae; these two strains showed 95.7 % sequence similarity, while similarities between P31T and other members of the genus Parvibaculum were below 93 %. Based on the genotypic and phenotypic data, strain P31T represents a novel species of the genus Parvibaculum, for which the name Parvibaculum indicum sp. nov. is proposed. The type strain is P31T (=CCTCC AB 208230T =LMG 24712T =MCCC 1A01132T).

scholarly journals Citreicella marina sp. nov., isolated from deep-sea sediment

2011 ◽  
Vol 61 (4) ◽  
pp. 728-731 ◽  
Author(s):  
Qiliang Lai ◽  
Yuanyuan Fu ◽  
Jianning Wang ◽  
Shuangxi Chen ◽  
Huanzi Zhong ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Deep Sea ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Chromosomal Dna ◽  
Phenotypic Data ◽  
Deep Sea Sediment ◽  
South West Indian Ocean ◽  
West Indian Ocean ◽  
Ocean Ridge

A taxonomic study was carried out on a novel strain, designated CK-I3-6T, which was isolated from deep-sea sediment of the south-west Indian Ocean Ridge. Cells were Gram-reaction-negative, oxidase- and catalase-positive, rod-shaped and non-motile. Growth was observed at 4–38 °C and in 1–12 % (w/v) NaCl. Cells were able to degrade gelatin and oxidize thiosulfate but did not reduce nitrate. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CK-I3-6T belonged to the genus Citreicella with a sequence similarity of 97.3 % to Citreicella thiooxidans CHLG 1T, while similarities with other taxa were <95.7 %. DNA–DNA hybridization showed that strain CK-I3-6T and C. thiooxidans CHLG 1T showed a low DNA–DNA relatedness (48±3 %). The principal fatty acids were C16 : 0 (7.8 %), C18 : 1ω7c (66.6 %), summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c; 6.3 %) and C19 : 0ω8c cyclo (10.0 %). The chromosomal DNA G+C content was 67.5 mol%. On the basis of the combined genotypic and phenotypic data, strain CK-I3-6T represents a novel species of the genus Citreicella, for which the name Citreicella marina sp. nov. is proposed. The type strain is CK-I3-6T ( = CCTCC AB 209064T  = LMG 25230T  = MCCC 1A03060T).

scholarly journals Aestuariivita atlantica sp. nov., isolated from deep-sea sediment

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3281-3285 ◽  
Author(s):  
Guizhen Li ◽  
Qiliang Lai ◽  
Yaping Du ◽  
Xiupian Liu ◽  
Fengqin Sun ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Deep Sea ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Chromosomal Dna ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Respiratory Quinone ◽  
Deep Sea Sediment ◽  
Gram Staining

A novel strain, 22II-S11-z3T, was isolated from the deep-sea sediment of the Atlantic Ocean. The bacterium was aerobic, Gram-staining-negative, oxidase-positive and catalase-negative, oval- to rod-shaped, and non-motile. Growth was observed at salinities of 1–9 % NaCl and temperatures of 10–45 °C. The isolate could hydrolyse aesculin and Tweens 20, 40 and 80, but not gelatin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II-S11-z3T belonged to the genus Aestuariivita, with highest sequence similarity to Aestuariivita boseongensis KCTC 42052T (97.5 %). The average nucleotide identity and digital DNA–DNA hybridization values between strain 22II-S11-z3T and A. boseongensis KCTC 42052T were 71.5 % and 20.0 ± 2.3 %, respectively. The G+C content of the chromosomal DNA was 65.5 mol%. The principal fatty acids (>5 %) were summed feature 8 (C18 : 1ω7c/ω6c) (35.2 %), C19 : 0 cyclo ω8c (20.9 %), C16 : 0 (11.8 %), 11-methyl C18 : 1ω7c (11.4 %) and C12 : 1 3-OH (9.4 %). The respiratory quinone was determined to be Q-10. Diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, nine unidentified phospholipids, one unidentified aminolipid and two unidentified lipids were present. The combined genotypic and phenotypic data show that strain 22II-S11-z3T represents a novel species of the genus Aestuariivita, for which the name Aestuariivita atlantica sp. nov. is proposed, with the type strain 22II-S11-z3T ( = KCTC 42276T = MCCC 1A09432T).

scholarly journals Marinibacterium profundimaris gen. nov., sp. nov., isolated from deep seawater

2015 ◽  
Vol 65 (Pt_11) ◽  
pp. 4175-4179 ◽  
Author(s):  
Guizhen Li ◽  
Qiliang Lai ◽  
Yaping Du ◽  
Xiupian Liu ◽  
Fengqin Sun ◽  
...  
Keyword(s):  
New Genus ◽  
Novel Species ◽  
Sequence Similarity ◽  
Tween 80 ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Deep Seawater ◽  
Phenotypic Data ◽  
Respiratory Quinone ◽  
The Family

A taxonomic study was carried out on strain 22II1-22F33T, which was isolated from deep seawater of the Atlantic Ocean. The bacterium was Gram-stain-negative, oxidase-positive and weakly catalase-positive, oval in shape without flagellum. Growth was observed at salinities of 0–12 % and at temperatures of 4–41 °C. The isolate was capable of hydrolysing aesculin and Tween 80 and reduction of nitrate to nitrite, but unable to hydrolyse gelatin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II1-22F33T belongs to the family Rhodobacteraceae, with highest sequence similarity to Pseudooceanicola marinus AZO-CT (96.5 %). The principal fatty acids (>10 %) were summed feature 8 (C18 : 1ω7c/ω6c) (73.8 %). The G+C content of the genomic DNA was 66.2 mol%. The respiratory quinone was Q-10 (100 %). Phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), two unidentified aminolipids (ALs), six unidentified phospholipids (PLs) and one unidentified lipid (L) were present. The combined genotypic and phenotypic data show that strain 22II1-22F33T represents a novel species within a new genus, for which the name Marinibacterium profundimaris gen. nov., sp. nov. is proposed. The type strain of Marinibacterium profundimaris is 22II1-22F33T ( = LMG 27151T = MCCC 1A09326T).

scholarly journals Paramesonia marina gen. nov., sp. nov., isolated from deep-sea water of the Indian Ocean

2020 ◽  
Vol 70 (4) ◽  
pp. 2325-2333 ◽  
Author(s):  
Lei Wang ◽  
Qiliang Lai ◽  
Xiupian Liu ◽  
Zongze Shao
Keyword(s):  
Indian Ocean ◽  
Deep Sea ◽  
Sea Water ◽  
Rrna Gene ◽  
Chromosomal Dna ◽  
Phenotypic Data ◽  
Content Type ◽  
Deep Sea Water ◽  
Link Type ◽  
The Indian Ocean

A taxonomic study was carried out of strain K7T, which was isolated from deep-sea water collected from the Indian Ocean. The bacterium was Gram-stain-negative, aerobic, oxidase-negative, catalase-positive, rod-shaped and non-motile. Growth was observed at salinities of 0.5–10 % (optimum, 3 %), at a pH range of pH 6.0–10.0 (optimum, pH 7.0) and at temperatures of 10–40 °C (optimum, 28 °C). Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K7T belonged to the family Flavobacteriaceae , with the high sequence similarities to the genera Mesonia (92.2 %–94.4 %), Salinimicrobium (91.9 %–93.2 %), Salegentibacter (92.1 %–92.6 %), Leeuwenhoekiella (92.1 %–92.3 %), Gramella (91.9 %–92.1 %) and Zunongwangia (91.8 %–92.1 %). The principal fatty acids were iso-C15 : 0 (28.4 %), iso-C15 : 1G (14.2 %), summed feature 9 (iso-C17 : 1  ω9c and/or C16 : 0 10-methyl; 11.6 %), iso-C17 : 0 3-OH (10.0 %) and summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c; 9.6 %). The G+C content of the chromosomal DNA was 35.8 mol%. The respiratory quinone was determined to be MK-6 (100 %). Phosphatidylethanolamine, two unidentified aminolipids, two unidentified phospholipid and four unidentified lipids were detected. The combined genotypic and phenotypic data show that strain K7T represents a novel species of a novel genus, for which the name Paramesonia marina gen. nov., sp. nov. is proposed, with the type strain K7T (=MCCC 1A01093T=KCTC 52325T).

scholarly journals Roseovarius indicus sp. nov., isolated from deep-sea water of the Indian Ocean

2011 ◽  
Vol 61 (9) ◽  
pp. 2040-2044 ◽  
Author(s):  
Qiliang Lai ◽  
Huanzi Zhong ◽  
Jianning Wang ◽  
Jun Yuan ◽  
Fengqin Sun ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Indian Ocean ◽  
16S Rrna ◽  
Deep Sea ◽  
Sea Water ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Chromosomal Dna ◽  
Deep Sea Water ◽  
The Indian Ocean

A taxonomic study was carried out on a novel bacterial strain, designated B108T, which was isolated from a polycyclic aromatic hydrocarbon (PAH)-degrading consortium, enriched from deep-sea water of the Indian Ocean. The isolate was Gram-reaction-negative, rod-shaped and non-motile. Growth of strain B108T was observed in 1–15 % (w/v) NaCl and at 10–39 °C and it was unable to degrade Tween 80 or gelatin. 16S rRNA gene sequence comparisons showed that strain B108T was most closely related to Roseovarius halotolerans HJ50T (97.1 % sequence similarity), followed by Roseovarius pacificus 81-2T (96.6 %) and Roseovarius aestuarii SMK-122T (95.2 %); other species shared <95.0 % sequence similarity. DNA–DNA hybridization tests showed that strain B108T had a low DNA–DNA relatedness to R. halotolerans HJ50T and R. pacificus 81-2T (48±4 % and 44±5 %, respectively). The predominant fatty acids were C16 : 0, C16 : 0 2-OH, summed feature 8 (C18 : 1ω7c/ω6c) and C19 : 0ω8c cyclo, which accounted for 84.2 % of the total cellular fatty acids. The G+C content of the chromosomal DNA was 63.6 mol%. The major respiratory quinone was ubiquinone 10 (Q10). Phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and some unidentified compounds were detected. These characteristics were in good agreement with those of members of the genus Roseovarius. The pufLM gene was also detected. According to its morphology, physiology, fatty acid composition and phylogenetic position based on 16S rRNA sequence data, the novel strain most appropriately belongs to the genus Roseovarius but can be readily distinguished from known species of this genus. Therefore, strain B108T represents a novel species, of the genus Roseovarius, for which the name Roseovarius indicus sp. nov. is proposed. The type strain is B108T ( = 2PR52-14T  = CCTCC AB 208233T  = LMG 24622T  = MCCC 1A01227T).

scholarly journals Pararhodobacter marinus sp. nov., isolated from deep-sea water of the Indian Ocean

2019 ◽  
Vol 69 (4) ◽  
pp. 932-936 ◽  
Author(s):  
Qiliang Lai ◽  
Xiupian Liu ◽  
Jun Yuan ◽  
Shuchen Xie ◽  
Zongze Shao
Keyword(s):  
Indian Ocean ◽  
Deep Sea ◽  
Type Species ◽  
Sea Water ◽  
Rrna Gene ◽  
Chromosomal Dna ◽  
Content Type ◽  
Deep Sea Water ◽  
Link Type ◽  
The Indian Ocean

A taxonomic study was carried out on strain CIC4N-9T, which was isolated from deep-sea water of the Indian Ocean. The bacterium was Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and non-motile. Growth was observed at salinities of 0–9% and at temperatures of 4–41 °C. The isolate was able to degrade gelatin but not aesculin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CIC4N-9T belonged to the genus Pararhodobacter , with the highest sequence similarity to the only recognized species, Pararhodobacter aggregans D1-19T (96.9 %). The average nucleotide identity and estimated DNA–DNA hybridization values between strain CIC4N-9T and P. aggregans D1-19T were 80.4 and 23.0 %, respectively. The principal fatty acids were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), C16 : 0, C18 : 1ω7c 11-methyl, C18 : 0 and C17 : 0. The G+C content of the chromosomal DNA was 66.8 mol%. The sole respiratory quinone was determined to be Q-10. Phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, two unknown phospholipids, four unknown aminolipids and one unknown polar lipid were present. The combined genotypic and phenotypic data show that strain CIC4N-9T represents a novel species within the genus Pararhodobacter , for which the name Pararhodobacter marinus sp. nov. is proposed. The type strain is CIC4N-9T (=MCCC 1A01225T=KCTC 52336T).

scholarly journals Solimonas marina sp. nov., isolated from deep seawater of the Pacific Ocean

2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Xiupian Liu ◽  
Qiliang Lai ◽  
Fengqin Sun ◽  
Yaping Du ◽  
Yingbao Gai ◽  
...  
Keyword(s):  
Pacific Ocean ◽  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Chromosomal Dna ◽  
Deep Seawater ◽  
Content Type ◽  
Link Type ◽  
The Pacific ◽  
The Pacific Ocean

A taxonomic study was carried out on strain C16B3T, which was isolated from deep seawater of the Pacific Ocean. The bacterium was Gram-stain-negative, oxidase- and catalase- positive and rod-shaped. Growth was observed at salinities of 0–8.0 % and at temperatures of 10–45 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain C16B3T belonged to the genus Solimonas , with the highest sequence similarity to Solimonas terrae KIS83-12T (97.2 %), followed by Solimonas variicoloris MN28T (97.0 %) and the other four species of the genus Solimonas (94.5 –96.8 %). The average nucleotide identity and estimated DNA–DNA hybridization values between strain C16B3T and the type strains of the genus Solimonas were 74.05−79.48 % and 19.5–22.5 %, respectively. The principal fatty acids (>5 %) were summed feature 8 (C18 : 1  ω7c/C18 : 1  ω6c; 20.9 %), iso-C16 : 0 (14.6 %), C16 : 1  ω5c (9.4 %), iso-C12 : 0 (8.4 %), summed feature 2 (C14 : 0 3-OH/iso I-C16 : 1 and C12 : 0 aldehyde; 6.8 %) and C16 : 0 (5.5 %). The G+C content of the chromosomal DNA was 65.37 mol%. The respiratory quinone was determined to be Q-8 (100 %). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, four unidentified aminolipids, six unidentified phospholipids and one unidentified polar lipid. The combined genotypic and phenotypic data show that strain C16B3T represents a novel species within the genus Solimonas , for which the name Solimonas marina sp. nov. is proposed, with the type strain C16B3T (=MCCC 1A04678T=KCTC 52314T).

Gulosibacter sediminis sp. nov., isolated from Indian Ocean marine sediment

2021 ◽  
Vol 71 (7) ◽  
Author(s):  
Zhao Jiang ◽  
Wei-Hua Zhang ◽  
Dan Song ◽  
Min Xiao ◽  
Dorji Phurbu ◽  
...  
Keyword(s):  
Indian Ocean ◽  
Marine Sediment ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Phenotypic Data ◽  
Content Type ◽  
Link Type ◽  
Marine Sediment Sample ◽  
Unknown Lipid ◽  
Unknown Phospholipid

A novel Gram-stain-positive, catalase-positive, oxidase-negative, aerobic, non-motile, rod-shaped bacterium, designated strain YIM M12148T, was isolated from a marine sediment sample collected from the Indian Ocean. The strain grew optimally at 28 °C, pH 8.0 and in the presence of 1–3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM M12148T belongs to the genus Gulosibacter , with the highest sequence similarity to Gulosibacter faecalis NBRC 15706T (96.12 %). The cell-wall sugars of strain YIM M12148T were rhamnose, ribose, glucose and mannose. The predominant isoprenoid quinones were MK-8 and MK-9. The polar lipids consisted of major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid and one unknown lipid. Major fatty acids (>5 % of the total) of the novel isolate were anteiso-C15 : 0, iso-C15 : 0, iso-C13 : 0 and anteiso-C13 : 0. The genomic DNA G+C content of strain YIM M12148T was 67.15 mol%. On the basis of genotypic and phenotypic data, it is apparent that strain YIM M12148T represents a novel species of the genus Gulosibacter , for which the name Gulosibacter sediminis sp. nov. is proposed. The type strain is YIM M12148T (=KCTC 29660T=DSM 29154T).

Sulfurovum indicum sp. nov., a novel hydrogen- and sulfur-oxidizing chemolithoautotroph isolated from a deep-sea hydrothermal plume in the Northwestern Indian Ocean

2019 ◽  
Vol 71 (3) ◽  
Author(s):  
Shaobin Xie ◽  
Shasha Wang ◽  
Dengfeng Li ◽  
Zongze Shao ◽  
Qiliang Lai ◽  
...  
Keyword(s):  
Indian Ocean ◽  
Deep Sea ◽  
Type Species ◽  
Elemental Sulfur ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Phenotypic Data ◽  
Content Type ◽  
Link Type ◽  
Sulfur Oxidizing

A novel mesophilic, hydrogen-, and sulfur-oxidizing bacterium, designated strain ST-419T, was isolated from a deep-sea hydrothermal vent plume on the Carlsberg Ridge of the Northwestern Indian Ocean. The isolate was a Gram-staining-negative, non-motile and coccoid to oval-shaped bacterium. Growth was observed at 4–50 °C (optimum 37 °C), pH 5.0–8.6 (optimum pH 6.0) and 1.0–5.0 % (w/v) NaCl (optimum 3.0 %). ST-419T could grow chemlithoautotrophically with molecular hydrogen, sulfide, elemental sulfur and thiosulfate as energy sources. Molecular oxygen, nitrate and elemental sulfur could be used as electron acceptors. The predominant fatty acids were C16 : 1ω7c, C18 : 1ω7c and C16 : 0. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The respiratory quinone was menaquinone MK-6 and the G+C content of the genomic DNA was 42.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that ST-419T represented a member of genus Sulfurovum and was most closely related to Sulfurovum riftiae 1812ET, with 97.6 % sequence similarity. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between ST-419T and S. riftiae 1812ET were 74.6 and 19.6 %, respectively. The combined genotypic and phenotypic data indicate that ST-419T represents a novel species within the genus Sulfurovum , for which the name Sulfurovum indicum sp. nov. is proposed. The type strain is ST-419T (=MCCC 1A17954T=KCTC 25164T).

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