scholarly journals Mesorhizobium camelthorni sp. nov., isolated from Alhagi sparsifolia

2011 ◽  
Vol 61 (3) ◽  
pp. 574-579 ◽  
Author(s):  
Wei-Min Chen ◽  
Wen-Fei Zhu ◽  
Cyril Bontemps ◽  
J. Peter W. Young ◽  
Ge-Hong Wei
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Type Strain ◽  
Novel Species ◽  
Root Nodules ◽  
Phylogenetic Analyses ◽  
Physiological Characteristics ◽  
Alhagi Sparsifolia ◽  
Dna Relatedness ◽  
Core Genes

Nine strains isolated from symbiotic root nodules on Alhagi sparsifolia were previously designated as representing genospecies I. Phylogenetic analyses indicated that genospecies I was related closely to Mesorhizobium alhagi (genospecies II), and clearly formed a new lineage within the genus Mesorhizobium. In this study, we differentiated genospecies I from recognized species of the genus Mesorhizobium based on phylogenetic analyses of additional core genes (recA, glnA), levels of DNA–DNA relatedness (<43.3 %), fatty acid profile (58 % C18 : 1 ω7c, 19 % 11-methyl C18 : 1 ω7c), and biochemical and physiological characteristics. The nine strains are therefore considered to represent a novel species of the genus Mesorhizobium, for which the name Mesorhizobium camelthorni sp. nov. is proposed. The type strain is CCNWXJ 40-4T (=HAMBI 3020T =ACCC 14549T).

scholarly journals Lentzea kentuckyensis sp. nov., of equine origin

2007 ◽  
Vol 57 (8) ◽  
pp. 1780-1783 ◽  
Author(s):  
D. P. Labeda ◽  
J. M. Donahue ◽  
S. F. Sells ◽  
R. M. Kroppenstedt
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Diagnostic Tests ◽  
Type Strain ◽  
Novel Species ◽  
Physiological Characteristics ◽  
Morphological Similarity ◽  
Preliminary Study

A novel actinomycete, designated strain LDDC 2876-05T, was isolated from an equine placenta during the course of routine diagnostic tests for nocardioform placentitis. In a preliminary study, the strain was observed to be phylogenetically distinct from the genera Crossiella and Amycolatopsis and probably a member of the genus Lentzea. A polyphasic study of strain LDDC 2876-05T confirmed its identification as a member of Lentzea on the basis of its chemotaxonomic and morphological similarity to all of the known species of the genus. Moreover, the strain could be distinguished from other species with validly published names on the basis of its phylogenetic and physiological characteristics and its fatty acid profile. Therefore strain LDDC 2876-05T represents a novel species of the genus Lentzea, for which the name Lentzea kentuckyensis sp. nov. is proposed. The type strain is LDDC 2876-05T (=NRRL B-24416T =DSM 44909T).

scholarly journals Alteromonas hispanica sp. nov., a polyunsaturated-fatty-acid-producing, halophilic bacterium isolated from Fuente de Piedra, southern Spain

2005 ◽  
Vol 55 (6) ◽  
pp. 2385-2390 ◽  
Author(s):  
Fernando Martínez-Checa ◽  
Victoria Béjar ◽  
Inmaculada Llamas ◽  
Ana del Moral ◽  
Emilia Quesada
Keyword(s):  
Fatty Acid ◽  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Halophilic Bacterium ◽  
Southern Spain ◽  
Isoprenoid Quinone ◽  
Hypersaline Water ◽  
Phenotypic Tests ◽  
Major Isoprenoid Quinone

Strain F-32T, which produces exopolysaccharides and contains polyunsaturated fatty acids, was isolated from a hypersaline water sample collected from Fuente de Piedra (southern Spain). Phylogenetic analyses indicated conclusively that the strain in question belonged to the genus Alteromonas. Phenotypic tests showed that it could be assigned to the genus Alteromonas although it had a number of distinctive characteristics: it is moderately halophilic, growing best with 7·5–10 % w/v NaCl; it grows at 4 °C and produces H2S; it does not grow with d-cellobiose, d-fructose, d-galactose, d-glucose or lactose as sole sources of carbon and energy; and its fatty-acid profile is typical of Alteromonas but it also contains a large amount of an unusual acid with three double bonds [18 : 3ω6c (6, 9, 12); 5·01 %, w/v]. The major isoprenoid quinone is Q8. The DNA G+C content is 46·3 mol%. The phylogenetic, phenotypic and genetic properties of strain F-32T place it within a novel species, for which the name Alteromonas hispanica sp. nov. is proposed. The type strain is F-32T (=CECT 7067T=LMG 22958T).

scholarly journals Rhizobium desertarenae sp. nov., isolated from the Saline Desert Soil from the Rann of Kachchh, India

2020 ◽  
Author(s):  
Mitesh Khairnar ◽  
Ashwini Hagir ◽  
Avinash Narayan ◽  
Kunal Jain ◽  
Datta Madamwar ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Desert Soil ◽  
Rrna Gene ◽  
The Family ◽  
Saline Desert ◽  
Distinct Branch ◽  
Core Genes

AbstractA novel bacterial strain designated ADMK78T was isolated from the saline desert soil. The cells were rod-shaped, Gram-negative, and non-motile. The strain ADMK78T grows best at 28°C and pH 7.0 and can tolerate up to 2% (w/v) NaCl. Based on 16S rRNA gene phylogeny, the strain ADMK78T belongs to the genus Rhizobium, with the highest similarity to Rhizobium wuzhouense W44T (98.7%) and Rhizobium ipomoeae shin9-1T (97.9%). Core-genes based phylogenetic analysis revealed that the strain ADMK78T forms a distinct branch in between Rhizobium ipomoeae shin9-1T and Rhizobium selenitireducens BAA-1503T. The average nucleotide identity of ADMK78T was less than 82%, to members of the family Rhizobiaceae. The genomic DNA G+C content of strain ADMK78T is 58.6 mol%. The major fatty acids of strain ADMK78T were C18:0 and C18:1 ω7c. The strain ADMK78T showed differences in physiological, phenotypic, and protein profiles estimated by MALDI-TOF MS to its closest relatives. Based on the phenotypic, chemotaxonomic properties, and phylogenetic analyses, the strain ADMK78T could be distinguished from the recognized species of the genus Rhizobium. It is suggested to represent a novel species of this genus, for which the name Rhizobium desertarenae sp. nov. is proposed. The type strain is ADMK78T (=MCC 3400T; KACC 21383T; JCM 33657T).

scholarly journals Oceanicola marinus sp. nov., a marine alphaproteobacterium isolated from seawater collected off Taiwan

2007 ◽  
Vol 57 (7) ◽  
pp. 1625-1629 ◽  
Author(s):  
Kuan-Yin Lin ◽  
Shih-Yi Sheu ◽  
Poh-Shing Chang ◽  
Jang-Cheon Cho ◽  
Wen-Ming Chen
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Dna Relatedness ◽  
Evolutionary Radiation ◽  
Southern Taiwan ◽  
Monophyletic Branch

A short-rod-shaped, Gram-negative, non-motile bacterial strain, designated AZO-CT, was isolated from a sample of seawater collected from the Eluanbi coast of Pingtung County in southern Taiwan and was characterized by using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus Oceanicola in the order Rhodobacterales of the Alphaproteobacteria. The closest neighbours were Oceanicola batsensis HTCC2597T (95.6 % similarity), Oceanicola nanhaiensis SS011B1-20T (94.5 %) and Oceanicola granulosus HTCC2516T (94.0 %). The predominant fatty acid was 18 : 1ω7c (49.1 %), and significant amounts of 19 : 0 cyclo (24.6 %) and 16 : 0 (14.7 %) were present. The DNA–DNA relatedness of the strain with respect to recognized species of the genus Oceanicola was less than 70 %. The isolate was also distinguishable from members of the genus Oceanicola on the basis of phenotypic and biochemical characteristics. It is evident from the genotypic, chemotaxonomic and phenotypic data, therefore, that strain AZO-CT represents a novel species of the genus Oceanicola, for which the name Oceanicola marinus sp. nov. is proposed. The type strain is AZO-CT (=LMG 23705T=BCRC 17591T).

scholarly journals Myroides pelagicus sp. nov., isolated from seawater in Thailand

2006 ◽  
Vol 56 (8) ◽  
pp. 1917-1920 ◽  
Author(s):  
Jaewoo Yoon ◽  
Suppasil Maneerat ◽  
Fusako Kawai ◽  
Akira Yokota
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Taxonomic Study ◽  
The Novel ◽  
16S Rrna Gene Sequences ◽  
Dna Relatedness ◽  
Myroides Odoratimimus

An aerobic, Gram-negative, non-motile, yellow-to-orange pigmented and rod-shaped bacterium, designated strain SM1T, was isolated from seawater in Thailand and subjected to a polyphasic taxonomic study. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the novel isolate shared 93–95 % sequence similarity with species of the genus Myroides. The DNA–DNA relatedness values of strain SM1T with Myroides odoratimimus JCM 7460T and Myroides odoratus JCM 7458T were below 70 %. The DNA G+C content of strain SM1T was 33.6 mol%, the major menaquinone was MK-6 and the major fatty acids were C15 : 0 iso, C17 : 1 ω9c iso and C17 : 0 iso 3-OH. Based on its phenotypic and chemotaxonomic characteristics, strain SM1T was classified as representing a novel species of the genus Myroides, for which the name Myroides pelagicus sp. nov. is proposed. The type strain is SM1T (=IAM 15337T=KCTC 12661T).

scholarly journals Mesonia hippocampi sp. nov., isolated from the brood pouch of a diseased Barbour's Seahorse (Hippocampus barbouri)

2015 ◽  
Vol 65 (Pt_7) ◽  
pp. 2241-2247 ◽  
Author(s):  
Judy Kolberg ◽  
Hans-Jürgen Busse ◽  
Thomas Wilke ◽  
Patrick Schubert ◽  
Peter Kämpfer ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Type Strain ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Brood Pouch ◽  
Diaminopimelic Acid ◽  
Sugar Residue ◽  
Rrna Gene ◽  
Polar Lipid Profile ◽  
Monophyletic Cluster

An orange-pigmented, Gram-staining-negative, rod-shaped bacterium, designated 96_Hippo_TS_3/13T was isolated from the brood pouch of a diseased seahorse male of the species Hippocampus barbouri from the animal facility of the University of Giessen, Germany. Phylogenetic analyses based on the nearly full-length 16S rRNA gene sequence placed strain 96_Hippo_TS_3/13T into the monophyletic cluster of the genus Mesonia within the family Flavobacteriaceae. However, the strain shared only 92.2–93.8 % sequence similarity to type strains of species of the genus Mesonia, with highest sequence similarity to the type strain of Mesonia aquimarina. Cellular fatty acid analysis showed a Mesonia-typical fatty acid profile including several branched and hydroxyl fatty acids with highest amounts of iso-C15 : 0 (40.9 %) followed by iso-C17 : 0 3-OH (14.8 %). In the polyamine pattern, sym-homospermidine was predominant. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The quinone system contained exclusively menaquinone MK-6. The only identified compound in the polar lipid profile was phosphatidylethanolamine present in major amounts. Additionally, major amounts of an unidentified aminolipid and two unidentified lipids not containing a phosphate group, an amino group or a sugar residue were detected. The genomic G+C content of strain 96_Hippo_TS_3/13T was 30 mol%. Based on genotypic, chemotaxonomic and physiological characterizations we propose a novel species of the genus Mesonia, Mesonia hippocampi sp. nov., with strain 96_Hippo_TS_3/13T ( = CIP 110839T =  LMG 28572T = CCM 8557T) as the type strain. An emended description of the genus Mesonia is also provided.

scholarly journals Dioszegia antarctica sp. nov. and Dioszegia cryoxerica sp. nov., psychrophilic basidiomycetous yeasts from polar desert soils in Antarctica

2010 ◽  
Vol 60 (6) ◽  
pp. 1466-1472 ◽  
Author(s):  
Laurie B. Connell ◽  
Regina Redman ◽  
Russel Rodriguez ◽  
Anne Barrett ◽  
Melissa Iszard ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Fungal Population ◽  
Desert Soils ◽  
Polar Desert ◽  
Yeast Strains ◽  
Victoria Land ◽  
Lsu Rrna Gene

During a survey of the culturable soil fungal population in samples collected in Taylor Valley, South Victoria Land, Antarctica, 13 basidiomycetous yeast strains with orange-coloured colonies were isolated. Phylogenetic analyses of internal transcribed spacer (ITS) and partial LSU rRNA gene sequences showed that the strains belong to the Dioszegia clade of the Tremellales (Tremellomycetes, Agaricomycotina), but did not correspond to any of the hitherto recognized species. Two novel species, Dioszegia antarctica sp. nov. (type strain ANT-03-116T =CBS 10920T =PYCC 5970T) and Dioszegia cryoxerica sp. nov. (type strain ANT-03-071T =CBS 10919T =PYCC 5967T), are described to accommodate ten and three of these strains, respectively. Analysis of ITS sequences demonstrated intrastrain sequence heterogeneity in D. cryoxerica. The latter species is also notable for producing true hyphae with clamp connections and haustoria. However, no sexual structures were observed. The two novel species can be considered obligate psychrophiles, since they failed to grow above 20 °C and grew best between 10 and 15 °C.

scholarly journals Enterococcus camelliae sp. nov., isolated from fermented tea leaves in Thailand

2007 ◽  
Vol 57 (9) ◽  
pp. 2151-2154 ◽  
Author(s):  
Sirapan Sukontasing ◽  
Somboon Tanasupawat ◽  
Somporn Moonmangmee ◽  
Jung-Sook Lee ◽  
Ken-ichiro Suzuki
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Unsaturated Fatty Acids ◽  
Novel Species ◽  
Tea Leaves ◽  
Phenotypic Characteristics ◽  
Straight Chain ◽  
Dna Relatedness ◽  
Gene Sequence Analysis ◽  
Enterococcus Italicus

A Gram-positive and catalase-negative coccus that formed chains, strain FP15-1T, isolated from fermented tea leaves (‘miang’), was studied systematically. The strain was facultatively anaerobic and produced l-lactic acid from glucose. Demethylmenaquinone (DMK-7) was the major menaquinone. Straight-chain unsaturated fatty acids C16 : 1 and C18 : 1 were the dominant components. The DNA G+C content was 37.8 mol%. On the basis of 16S rRNA and RNA polymerase α subunit (rpoA) gene sequence analysis, strain FP15-1T was closely related to Enterococcus italicus KCTC 5373T, with 99.2 and 93.8 % similarity, respectively. The strain could be clearly distinguished from E. italicus ATCC 5373T by low DNA–DNA relatedness (≤33.8 %) and phenotypic characteristics. Therefore, this strain represent a novel species of the genus Enterococcus, for which the name Enterococcus camelliae sp. nov. is proposed. The type strain is FP15-1T (=KCTC 13133T =NBRC 101868T =NRIC 0105T =TISTR 932T =PCU 277T).

Arthrobacter cryoconiti sp. nov., a psychrophilic bacterium isolated from alpine glacier cryoconite

2012 ◽  
Vol 62 (2) ◽  
pp. 397-402 ◽  
Author(s):  
Rosa Margesin ◽  
Peter Schumann ◽  
De-Chao Zhang ◽  
Mersiha Redzic ◽  
Yu-Guang Zhou ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Psychrophilic Bacterium ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Polar Lipid Profile ◽  
Alpine Glacier ◽  
Cellular Fatty Acids

A Gram-stain-positive, aerobic, non-motile, psychrophilic bacterium, designated strain Cr6-08T, was isolated from alpine glacier cryoconite. Growth of strain Cr6-08T occurred at 1–25 °C. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain Cr6-08T is most closely related to members of the genus Arthrobacter. Strain Cr6-08T possessed chemotaxonomic properties consistent with those of the genus Arthrobacter, such as peptidoglycan type A3α (l-Lys–l-Ala4), MK-9(H2) as major menaquinone and anteiso- and iso-branched compounds (anteiso-C15 : 0 and iso-C15 : 0) as major cellular fatty acids. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown glycolipid and three unknown polar lipids. The genomic DNA G+C content of strain Cr6-08T was 57.3 mol%. On the basis of phenotypic and chemotaxonomic characteristics, phylogenetic analysis and DNA–DNA relatedness data, strain Cr6-08T is considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter cryoconiti sp. nov. is proposed. The type strain is Cr6-08T ( = DSM 23324T  = LMG 26052T  = CGMCC 1.10698T).

scholarly journals Lactobacillus farraginis sp. nov. and Lactobacillus parafarraginis sp. nov., heterofermentative lactobacilli isolated from a compost of distilled shochu residue

2007 ◽  
Vol 57 (4) ◽  
pp. 708-712 ◽  
Author(s):  
Akihito Endo ◽  
Sanae Okada
Keyword(s):  
Phylogenetic Analysis ◽  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Dna Relatedness ◽  
Clear Separation ◽  
Micro Organisms

Five strains of lactic acid bacteria were isolated from a compost of distilled shochu residue in Japan. The isolates were separated into two groups on the basis of 16S rRNA gene sequence similarity, and two subclusters were formed that comprised micro-organisms closely related to Lactobacillus buchneri, L. diolivorans, L. hilgardii, L. kefiri, L. parabuchneri and L. parakefiri. DNA–DNA relatedness results revealed that the isolates could be separated into two groups, and these groups correlated well with the subclusters generated using the phylogenetic analysis. Moreover, the levels of DNA–DNA relatedness showed clear separation of the two groups from their phylogenetic relatives. Therefore, the two groups represent two novel species, for which the names Lactobacillus farraginis sp. nov. (type strain NRIC 0676T=JCM 14108T=DSM 18382T) and Lactobacillus parafarraginis sp. nov. (type strain NRIC 0677T=JCM 14109T=DSM 18390T) are proposed.

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