scholarly journals Alteromonas hispanica sp. nov., a polyunsaturated-fatty-acid-producing, halophilic bacterium isolated from Fuente de Piedra, southern Spain

2005 ◽  
Vol 55 (6) ◽  
pp. 2385-2390 ◽  
Author(s):  
Fernando Martínez-Checa ◽  
Victoria Béjar ◽  
Inmaculada Llamas ◽  
Ana del Moral ◽  
Emilia Quesada
Keyword(s):  
Fatty Acid ◽  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Halophilic Bacterium ◽  
Southern Spain ◽  
Isoprenoid Quinone ◽  
Hypersaline Water ◽  
Phenotypic Tests ◽  
Major Isoprenoid Quinone

Strain F-32T, which produces exopolysaccharides and contains polyunsaturated fatty acids, was isolated from a hypersaline water sample collected from Fuente de Piedra (southern Spain). Phylogenetic analyses indicated conclusively that the strain in question belonged to the genus Alteromonas. Phenotypic tests showed that it could be assigned to the genus Alteromonas although it had a number of distinctive characteristics: it is moderately halophilic, growing best with 7·5–10 % w/v NaCl; it grows at 4 °C and produces H2S; it does not grow with d-cellobiose, d-fructose, d-galactose, d-glucose or lactose as sole sources of carbon and energy; and its fatty-acid profile is typical of Alteromonas but it also contains a large amount of an unusual acid with three double bonds [18 : 3ω6c (6, 9, 12); 5·01 %, w/v]. The major isoprenoid quinone is Q8. The DNA G+C content is 46·3 mol%. The phylogenetic, phenotypic and genetic properties of strain F-32T place it within a novel species, for which the name Alteromonas hispanica sp. nov. is proposed. The type strain is F-32T (=CECT 7067T=LMG 22958T).

scholarly journals Mucilaginibacter rigui sp. nov., isolated from wetland freshwater, and emended description of the genus Mucilaginibacter

2010 ◽  
Vol 60 (1) ◽  
pp. 134-139 ◽  
Author(s):  
Keun Sik Baik ◽  
Seong Chan Park ◽  
Eun Mi Kim ◽  
Chae Hong Lim ◽  
Chi Nam Seong
Keyword(s):  
Fatty Acids ◽  
Type Strain ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Isoprenoid Quinone ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Emended Description ◽  
Major Isoprenoid Quinone

A non-motile, rod-shaped bacterium, designated strain WPCB133T, was isolated from freshwater collected from the Woopo wetland (Republic of Korea). Cells were Gram-reaction-negative, aerobic and catalase- and oxidase-positive. The major fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The strain contained MK-7 as the major isoprenoid quinone. The DNA G+C content was 47 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain WPCB133T forms an independent lineage within the genus Mucilaginibacter. Strain WPCB133T was distantly related to Mucilaginibacter kameinonensis SCKT (94.7 % sequence similarity), Mucilaginibacter paludis TPT56T (94.5 %) and Mucilaginibacter gracilis TPT18T (94.4 %). Phenotypic characteristics distinguished strain WPCB133T from members of the genus Mucilaginibacter. On the basis of evidence presented in this study, strain WPCB133T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter rigui sp. nov. is proposed. The type strain is WPCB133T (=KCTC 12534T =NBRC 101115T). An emended description of the genus Mucilaginibacter is also proposed.

scholarly journals Bacillus velezensis sp. nov., a surfactant-producing bacterium isolated from the river Vélez in Málaga, southern Spain

2005 ◽  
Vol 55 (1) ◽  
pp. 191-195 ◽  
Author(s):  
Cristina Ruiz-García ◽  
Victoria Béjar ◽  
Fernando Martínez-Checa ◽  
Inmaculada Llamas ◽  
Emilia Quesada
Keyword(s):  
Dna Hybridization ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Southern Spain ◽  
Bacterial Strains ◽  
Bacillus Atrophaeus ◽  
Bacillus Velezensis ◽  
Phenotypic Characteristics ◽  
Bacillus Mojavensis ◽  
Phenotypic Tests

Two Gram-positive, endospore-forming bacterial strains, CR-502T and CR-14b, which produce surfactant molecules are described. Phenotypic tests and phylogenetic analyses showed these strains to be members of the genus Bacillus and related to the species Bacillus atrophaeus, Bacillus mojavensis, Bacillus subtilis, Bacillus vallismortis and Bacillus amyloliquefaciens, although they differ from these species in a number of phenotypic characteristics. DNA–DNA hybridization confirmed that they show less than 20 % hybridization with the above-mentioned species and therefore represent a novel species of Bacillus. The DNA G+C content is 46·4 mol% in strain CR-502T and 46·1 mol% in strain CR-14b. The main fatty acids in strain CR-502T are 15 : 0 anteiso (32·70 %), 15 : 0 iso (29·86 %) and 16 : 0 (13·41 %). The main quinone in strain CR-502T is MK-7 (96·6 %). In the light of the polyphasic evidence gathered in this study, it is proposed that these strains be classified as a novel species of the genus Bacillus, with the name Bacillus velezensis sp. nov. The type strain (CR-502T=CECT 5686T=LMG 22478T) was isolated from a brackish water sample taken from the river Vélez at Torredelmar in Málaga, southern Spain.

scholarly journals Leucosporidium drummii sp. nov., a member of the Microbotryomycetes isolated from soil

2012 ◽  
Vol 62 (Pt_3) ◽  
pp. 728-734 ◽  
Author(s):  
A. M. Yurkov ◽  
A. M. Schäfer ◽  
D. Begerow
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Its Region ◽  
Rrna Gene ◽  
Grassland Soil ◽  
Core Group ◽  
The Core ◽  
26S Rrna ◽  
Phenotypic Tests

Two strains of a novel teleomorphic basidiomycete were isolated from grassland soil. Standard phenotypic tests and phylogenetic analyses of 26S rRNA gene (D1/D2 domains) and ITS region sequences showed that the species belongs to the core group of the genus Leucosporidium. A novel species, Leucosporidium drummii sp. nov., is proposed to accommodate the two strains, with SEG-3-2-AY220T ( = CBS 11562T = MUCL 52878T) as the type strain. In addition, phylogenetic analysis revealed great genetic variability in the Leucosporidium scottii complex.

scholarly journals Chryseobacterium paridis sp. nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var. yunnanensis

2021 ◽  
Author(s):  
Zhen Zhang ◽  
Ling-Ling Yang ◽  
Cong-Jian Li ◽  
Xing-Wang Jiang ◽  
Xiao-Yang Zhi
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Bacterial Species ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
Isoprenoid Quinone ◽  
16S Rrna Gene Sequences ◽  
Gram Negative ◽  
Paris Polyphylla

Abstract A Gram-negative, yellow-pigmented, rod-shaped bacterial strain YIM B02567 T was isolated from the root of Paris polyphylla Smith var. yunnanensis in China. Strain YIM B02567 T grew optimally at 25–30°C and at pH 7.0 in the absence of NaCl on nutrient agar. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain YIM B02567 T belong to the genus Chryseobacterium , and closely related to Chryseobacterium piperi CTM T and Chryseobacterium soli DSM 19298 T . Whole genome sequencing indicated that the genome size was 4,774,612 bp and had a DNA G+C content of 34.5 %. Values of the ANI and the dDDH between strain YIM B02567 T and its closely related Chryseobacterium species were below 81.72 % and 24.7 %. Strain YIM B02567 T contained menaquinone-6 as the sole isoprenoid quinone, anteiso-C 15:0, iso-C 17:1 ω 9 c and iso-C 17:0 3-OH as major fatty acids and phosphatidylethanolamine as major polar lipid. Based on the polyphasic analyses, strain YIM B02567 T could be differentiated genotypically and phenotypically from recognized species of the genus Chryseobacterium . The isolate therefore represents a novel species, for which the name Chryseobacterium paridis sp. nov. is proposed. The type strain is YIM B02567 T (=CGMCC 1.18657 T =JCM 5426 T ).

scholarly journals Sphingomonas vulcanisoli sp. nov., isolated from soil of a lava forest

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3320-3325 ◽  
Author(s):  
Keun Chul Lee ◽  
Kwang Kyu Kim ◽  
Jong-Shik Kim ◽  
Dae-Shin Kim ◽  
Suk-Hyung Ko ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Isoprenoid Quinone ◽  
Gram Stain ◽  
Rrna Gene Sequencing

A Gram-stain-negative, non-motile, yellow-pigmented and rod-shaped bacterial strain, designated SN6-13T, was isolated from soil of the Gotjawal, lava forest, located in Jeju, Republic of Korea. Cells of strain SN6-13T were oxidase- and catalase-positive. The isolate contained Q-10 as the predominant isoprenoid quinone, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0 as the major fatty acids, sym-homospermidine as the major polyamine and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid, ninhydrinphosphatidylglycerol and two unidentified aminophospholipids as the polar lipids. The DNA G+C content was 64.6 mol%. In phylogenetic analyses based on 16S rRNA gene sequencing, strain SN6-13T was most closely related to Sphingomonas laterariae LNB2T (95.4 % sequence similarity) and formed a separate lineage in the genus Sphingomonas. Based on phenotypic, chemotaxonomic and phylogenetic characteristics, it is concluded that strain SN6-13T represents a novel species of the genus Sphingomonas, for which the name Sphingomonas vulcanisoli sp. nov. is proposed. The type strain is SN6-13T ( = KCTC 42454T = CECT 8804T).

scholarly journals Mesorhizobium camelthorni sp. nov., isolated from Alhagi sparsifolia

2011 ◽  
Vol 61 (3) ◽  
pp. 574-579 ◽  
Author(s):  
Wei-Min Chen ◽  
Wen-Fei Zhu ◽  
Cyril Bontemps ◽  
J. Peter W. Young ◽  
Ge-Hong Wei
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Type Strain ◽  
Novel Species ◽  
Root Nodules ◽  
Phylogenetic Analyses ◽  
Physiological Characteristics ◽  
Alhagi Sparsifolia ◽  
Dna Relatedness ◽  
Core Genes

Nine strains isolated from symbiotic root nodules on Alhagi sparsifolia were previously designated as representing genospecies I. Phylogenetic analyses indicated that genospecies I was related closely to Mesorhizobium alhagi (genospecies II), and clearly formed a new lineage within the genus Mesorhizobium. In this study, we differentiated genospecies I from recognized species of the genus Mesorhizobium based on phylogenetic analyses of additional core genes (recA, glnA), levels of DNA–DNA relatedness (<43.3 %), fatty acid profile (58 % C18 : 1 ω7c, 19 % 11-methyl C18 : 1 ω7c), and biochemical and physiological characteristics. The nine strains are therefore considered to represent a novel species of the genus Mesorhizobium, for which the name Mesorhizobium camelthorni sp. nov. is proposed. The type strain is CCNWXJ 40-4T (=HAMBI 3020T =ACCC 14549T).

scholarly journals Flavihumibacter petaseus gen. nov., sp. nov., isolated from soil of a subtropical rainforest

2010 ◽  
Vol 60 (7) ◽  
pp. 1609-1612 ◽  
Author(s):  
Nan Nan Zhang ◽  
Jian Hang Qu ◽  
Hong Li Yuan ◽  
Yan Mei Sun ◽  
Jin Shui Yang
Keyword(s):  
Type Strain ◽  
New Genus ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Isoprenoid Quinone ◽  
16S Rrna Gene Sequences ◽  
Cellular Fatty Acids ◽  
Major Isoprenoid Quinone ◽  
Is Strain

A yellow-coloured bacterium, T41T, was isolated from a soil sample of a subtropical rainforest in Nepal. Cells were Gram-reaction-positive, aerobic, non-motile, short rods. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain formed a cluster with Terrimonas ferruginea, Terrimonas lutea, Niabella soli, Flavisolibacter ginsengiterrae, Flavisolibacter ginsengisoli, Niastella yeongjuensis and Niastella koreensis in the phylum Bacteroidetes. The strain showed the highest sequence similarity to the type strain of Terrimonas lutea (93.2 %). The major isoprenoid quinone was MK-7 and the predominant cellular fatty acids (>10 %) were iso-15 : 0 (33.8 %), iso-15 : 1 G (13.3 %) and iso-17 : 0 3-OH (12.9 %). The DNA G+C content was 48.1 mol%. On the basis of phenotypic and phylogenetic data and genomic distinctiveness, strain T41T represents a novel species in a new genus in the phylum Bacteroidetes, for which the name Flavihumibacter petaseus gen. nov., sp. nov. is proposed. The type strain of Flavihumibacter petaseus is strain T41T (=CGMCC 1.7723T =NBRC 106054T).

scholarly journals Mesonia hippocampi sp. nov., isolated from the brood pouch of a diseased Barbour's Seahorse (Hippocampus barbouri)

2015 ◽  
Vol 65 (Pt_7) ◽  
pp. 2241-2247 ◽  
Author(s):  
Judy Kolberg ◽  
Hans-Jürgen Busse ◽  
Thomas Wilke ◽  
Patrick Schubert ◽  
Peter Kämpfer ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Type Strain ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Brood Pouch ◽  
Diaminopimelic Acid ◽  
Sugar Residue ◽  
Rrna Gene ◽  
Polar Lipid Profile ◽  
Monophyletic Cluster

An orange-pigmented, Gram-staining-negative, rod-shaped bacterium, designated 96_Hippo_TS_3/13T was isolated from the brood pouch of a diseased seahorse male of the species Hippocampus barbouri from the animal facility of the University of Giessen, Germany. Phylogenetic analyses based on the nearly full-length 16S rRNA gene sequence placed strain 96_Hippo_TS_3/13T into the monophyletic cluster of the genus Mesonia within the family Flavobacteriaceae. However, the strain shared only 92.2–93.8 % sequence similarity to type strains of species of the genus Mesonia, with highest sequence similarity to the type strain of Mesonia aquimarina. Cellular fatty acid analysis showed a Mesonia-typical fatty acid profile including several branched and hydroxyl fatty acids with highest amounts of iso-C15 : 0 (40.9 %) followed by iso-C17 : 0 3-OH (14.8 %). In the polyamine pattern, sym-homospermidine was predominant. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The quinone system contained exclusively menaquinone MK-6. The only identified compound in the polar lipid profile was phosphatidylethanolamine present in major amounts. Additionally, major amounts of an unidentified aminolipid and two unidentified lipids not containing a phosphate group, an amino group or a sugar residue were detected. The genomic G+C content of strain 96_Hippo_TS_3/13T was 30 mol%. Based on genotypic, chemotaxonomic and physiological characterizations we propose a novel species of the genus Mesonia, Mesonia hippocampi sp. nov., with strain 96_Hippo_TS_3/13T ( = CIP 110839T =  LMG 28572T = CCM 8557T) as the type strain. An emended description of the genus Mesonia is also provided.

scholarly journals Dioszegia antarctica sp. nov. and Dioszegia cryoxerica sp. nov., psychrophilic basidiomycetous yeasts from polar desert soils in Antarctica

2010 ◽  
Vol 60 (6) ◽  
pp. 1466-1472 ◽  
Author(s):  
Laurie B. Connell ◽  
Regina Redman ◽  
Russel Rodriguez ◽  
Anne Barrett ◽  
Melissa Iszard ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Fungal Population ◽  
Desert Soils ◽  
Polar Desert ◽  
Yeast Strains ◽  
Victoria Land ◽  
Lsu Rrna Gene

During a survey of the culturable soil fungal population in samples collected in Taylor Valley, South Victoria Land, Antarctica, 13 basidiomycetous yeast strains with orange-coloured colonies were isolated. Phylogenetic analyses of internal transcribed spacer (ITS) and partial LSU rRNA gene sequences showed that the strains belong to the Dioszegia clade of the Tremellales (Tremellomycetes, Agaricomycotina), but did not correspond to any of the hitherto recognized species. Two novel species, Dioszegia antarctica sp. nov. (type strain ANT-03-116T =CBS 10920T =PYCC 5970T) and Dioszegia cryoxerica sp. nov. (type strain ANT-03-071T =CBS 10919T =PYCC 5967T), are described to accommodate ten and three of these strains, respectively. Analysis of ITS sequences demonstrated intrastrain sequence heterogeneity in D. cryoxerica. The latter species is also notable for producing true hyphae with clamp connections and haustoria. However, no sexual structures were observed. The two novel species can be considered obligate psychrophiles, since they failed to grow above 20 °C and grew best between 10 and 15 °C.

Arthrobacter cryoconiti sp. nov., a psychrophilic bacterium isolated from alpine glacier cryoconite

2012 ◽  
Vol 62 (2) ◽  
pp. 397-402 ◽  
Author(s):  
Rosa Margesin ◽  
Peter Schumann ◽  
De-Chao Zhang ◽  
Mersiha Redzic ◽  
Yu-Guang Zhou ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Psychrophilic Bacterium ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Polar Lipid Profile ◽  
Alpine Glacier ◽  
Cellular Fatty Acids

A Gram-stain-positive, aerobic, non-motile, psychrophilic bacterium, designated strain Cr6-08T, was isolated from alpine glacier cryoconite. Growth of strain Cr6-08T occurred at 1–25 °C. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain Cr6-08T is most closely related to members of the genus Arthrobacter. Strain Cr6-08T possessed chemotaxonomic properties consistent with those of the genus Arthrobacter, such as peptidoglycan type A3α (l-Lys–l-Ala4), MK-9(H2) as major menaquinone and anteiso- and iso-branched compounds (anteiso-C15 : 0 and iso-C15 : 0) as major cellular fatty acids. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown glycolipid and three unknown polar lipids. The genomic DNA G+C content of strain Cr6-08T was 57.3 mol%. On the basis of phenotypic and chemotaxonomic characteristics, phylogenetic analysis and DNA–DNA relatedness data, strain Cr6-08T is considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter cryoconiti sp. nov. is proposed. The type strain is Cr6-08T ( = DSM 23324T  = LMG 26052T  = CGMCC 1.10698T).

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