Halovivax ruber sp. nov., an extremely halophilic archaeon isolated from Lake Xilinhot, Inner Mongolia, China

2007 ◽  
Vol 57 (5) ◽  
pp. 1024-1027 ◽  
Author(s):  
A. M. Castillo ◽  
M. C. Gutiérrez ◽  
M. Kamekura ◽  
Y. Xue ◽  
Y. Ma ◽  
...  
Keyword(s):  
Inner Mongolia ◽  
Type Strain ◽  
Lipid Analysis ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
Physiological Characterization ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Extremely Halophilic Archaeon ◽  
Type Strains

A Gram-negative, pleomorphic, extremely halophilic archaeon, designated strain XH-70T, was isolated from the saline Lake Xilinhot, in Inner Mongolia, China. It formed small (0.9–1.5 mm), red-pigmented, elevated colonies on agar medium. The strain required at least 2.5 M NaCl and 5 mM Mg2+ for growth. The 16S rRNA gene sequence analysis indicated that strain XH-70T belongs to the family Halobacteriaceae, showing 99.5 % similarity to the type strain of Halovivax asiaticus and 94.7 and 94.6 % similarity, respectively, to the type strains of Natronococcus amylolyticus and Natronococcus occultus. Polar lipid analysis supported the placement of strain XH-70T in the genus Halovivax. DNA–DNA hybridization studies (32 % with Halovivax asiaticus CGMCC 1.4248T), as well as biochemical and physiological characterization, allowed strain XH-70T to be differentiated from Halovivax asiaticus. A novel species, Halovivax ruber sp. nov., is therefore proposed to accommodate this strain. The type strain is XH-70T (=CGMCC 1.6204T=DSM 18193T=JCM 13892T).

scholarly journals Haloferax sulfurifontis sp. nov., a halophilic archaeon isolated from a sulfide- and sulfur-rich spring

2004 ◽  
Vol 54 (6) ◽  
pp. 2275-2279 ◽  
Author(s):  
Mostafa S. Elshahed ◽  
Kristen N. Savage ◽  
Aharon Oren ◽  
M. Carmen Gutierrez ◽  
Antonio Ventosa ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Lipid Analysis ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
Physiological Characterization ◽  
Gene Sequence Analysis ◽  
Wide Range ◽  
The Family ◽  
Extremely Halophilic Archaeon

A pleomorphic, extremely halophilic archaeon (strain M6T) was isolated from a sulfide- and sulfur-rich spring in south-western Oklahoma (USA). It formed small (0·8–1·0 mm), salmon pink, elevated colonies on agar medium. The strain grew in a wide range of NaCl concentrations (6 % to saturation) and required at least 1 mM Mg2+ for growth. Strain M6T was able to reduce sulfur to sulfide anaerobically. 16S rRNA gene sequence analysis indicated that strain M6T belongs to the family Halobacteriaceae, genus Haloferax; it showed 96·7–98·0 % similarity to other members of the genus with validly published names and 89 % similarity to Halogeometricum borinquense, its closest relative outside the genus Haloferax. Polar lipid analysis and DNA G+C content further supported placement of strain M6T in the genus Haloferax. DNA–DNA hybridization values, as well as biochemical and physiological characterization, allowed strain M6T to be differentiated from other members of the genus Haloferax. A novel species, Haloferax sulfurifontis sp. nov., is therefore proposed to accommodate the strain. The type strain is M6T (=JCM 12327T=CCM 7217T=DSM 16227T=CIP 108334T).

scholarly journals Natronoarchaeum mannanilyticum gen. nov., sp. nov., an aerobic, extremely halophilic archaeon isolated from commercial salt

2010 ◽  
Vol 60 (11) ◽  
pp. 2529-2534 ◽  
Author(s):  
Yasuhiro Shimane ◽  
Yuji Hatada ◽  
Hiroaki Minegishi ◽  
Toru Mizuki ◽  
Akinobu Echigo ◽  
...  
Keyword(s):  
New Genus ◽  
Lipid Analysis ◽  
Optimal Growth ◽  
Rrna Gene ◽  
New Genus And Species ◽  
Optimal Growth Temperature ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Extremely Halophilic Archaeon ◽  
Type Strains

Strain YSM-123T was isolated from commercial salt made from Japanese seawater in Niigata prefecture. Optimal NaCl and Mg2+ concentrations for growth were 4.0–4.5 M and 5 mM, respectively. The isolate was a mesophilic and slightly alkaliphilic haloarchaeon, whose optimal growth temperature and pH were 37 °C and pH 8.0–9.0. Phylogenetic analysis based on 16S rRNA gene sequence analysis suggested that strain YSM-123T is a member of the phylogenetic group defined by the family Halobacteriaceae, but there were low similarities to type strains of other genera of this family (≤90 %); for example, Halococcus (similarity <89 %), Halostagnicola (<89 %), Natronolimnobius (<89 %), Halobiforma (<90 %), Haloterrigena (<90 %), Halovivax (<90 %), Natrialba (<90 %), Natronobacterium (<90 %) and Natronococcus (<90 %). The G+C content of the DNA was 63 mol%. Polar lipid analysis revealed the presence of phosphatidylglycerol, phosphatidylglycerophosphate methyl ester, disulfated diglycosyl diether and an unknown glycolipid. On the basis of the data presented, we propose that strain YSM-123T should be placed in a new genus and species, Natronoarchaeum mannanilyticum gen. nov., sp. nov. The type strain of Natronoarchaeum mannanilyticum is strain YSM-123T (=JCM 16328T =CECT 7565T).

scholarly journals Halopiger aswanensis sp. nov., a polymer-producing and extremely halophilic archaeon isolated from hypersaline soil

2010 ◽  
Vol 60 (3) ◽  
pp. 633-637 ◽  
Author(s):  
F. F. Hezayen ◽  
M. C. Gutiérrez ◽  
A. Steinbüchel ◽  
B. J. Tindall ◽  
B. H. A. Rehm
Keyword(s):  
Lipid Analysis ◽  
Optimal Growth ◽  
Distinct Species ◽  
Rrna Gene ◽  
The Novel ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Extremely Halophilic Archaeon ◽  
Sulfated Glycolipid ◽  
Physiological And Biochemical

Strain 56T was isolated from a hypersaline soil in Aswan (Egypt). Cells were pleomorphic rods. The organism was neutrophilic, motile and required at least 1.7 M (10 % w/v) NaCl, but not MgCl2, for growth; optimal growth occurred at ≥3.8 M (≥22.5 %) NaCl. The strain was thermotolerant with an optimum temperature for growth of 40 °C, although growth was possible up to 55 °C. The G+C content of the DNA of the novel strain was 67.1 mol%.16S rRNA gene sequence analysis revealed that strain 56T was a member of the phyletic group defined by the family Halobacteriaceae, showing the highest similarity to Halopiger xanaduensis SH-6T (99 %) and the next highest similarity of 94 % to other members of the family Halobacteriaceae. DNA–DNA hybridization revealed 27 % relatedness between strain 56T and Hpg. xanaduensis SH-6T. Polar lipid analysis revealed the presence of the bis-sulfated glycolipid S2-DGD-1 as the sole glycolipid and the absence of the glycerol diether analogue phosphatidylglycerosulfate. Both C20 . 20 and C20 . 25 core lipids were present. Strain 56T accumulated large amounts of polyhydroxybutyrate and also secreted an exopolymer. Physiological and biochemical differences suggested that Hpg. xanaduanesis and strain 56T were sufficiently different to be separated into two distinct species. It is suggested that strain 56T represents a novel species of the genus Halopiger, for which the name Halopiger aswanensis sp. nov. is proposed. The type strain is strain 56T (=DSM 13151T=JCM 11628T).

scholarly journals Nocardia goodfellowii sp. nov. and Nocardia thraciensis sp. nov., isolated from soil

2012 ◽  
Vol 62 (Pt_6) ◽  
pp. 1228-1234 ◽  
Author(s):  
Anil Sazak ◽  
Nevzat Sahin ◽  
Mustafa Camas
Keyword(s):  
Type Strain ◽  
Type Species ◽  
Rrna Gene ◽  
Phenotypic Data ◽  
Content Type ◽  
Link Type ◽  
Soil Actinomycetes ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Type Strains

The taxonomic position of two soil actinomycetes, strains A2012T and A2019T, isolated from Turkish soils, was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that the strains belonged to the family Nocardiaceae . Strains A2012T and A2019T were most closely related to Nocardia caishijiensis DSM 44831T (98.9 %) and Nocardia mexicana CIP 108295T (98.6 %), respectively; similarity to other type strains of the genus Nocardia ranged from 96.9 to 97.9 %. However, DNA–DNA relatedness and phenotypic data demonstrated that strains A2012T and A2019T could be clearly distinguished from members of the most closely related Nocardia species. It is evident from the genotypic and phenotypic data that the two isolates represent two novel species of the genus Nocardia . It is proposed, therefore, that strains A2012T and A2019T be classified in the genus Nocardia as representatives of Nocardia goodfellowii sp. nov. (type strain A2012T = DSM 45516T = NRRL B-24833T = KCTC 19986T) and Nocardia thraciensis sp. nov. (type strain A2019T = DSM 45517T = NRRL B-24834T = KCTC 19985T), respectively.

scholarly journals Salarchaeum japonicum gen. nov., sp. nov., an aerobic, extremely halophilic member of the Archaea isolated from commercial salt

2011 ◽  
Vol 61 (9) ◽  
pp. 2266-2270 ◽  
Author(s):  
Yasuhiro Shimane ◽  
Yuji Hatada ◽  
Hiroaki Minegishi ◽  
Akinobu Echigo ◽  
Syuhei Nagaoka ◽  
...  
Keyword(s):  
New Genus ◽  
Gene Sequence ◽  
Lipid Analysis ◽  
Sequence Similarity ◽  
Maximum Growth ◽  
Rrna Gene ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Optimum Ph ◽  
Extremely Halophilic Archaeon

Strain YSM-79T was isolated from commercial salt made from seawater in Yonaguni island, Okinawa, Japan. The strain is an aerobic, Gram-negative, chemo-organotrophic and extremely halophilic archaeon. Cells are short rods that lyse in distilled water. Growth occurs at 1.5–5.3 M NaCl (optimum 2.5–3.0 M), pH 5.0–8.8 (optimum pH 5.2–6.3) and 20–55 °C (optimum 40 °C). Mg2+ is required for growth, with maximum growth at 200–300 mM Mg2+. Polar lipid analysis revealed the presence of phosphatidylglycerol, phosphatidylglycerophosphate methyl ester, sulfated diglycosyl diether-1 and five unidentified glycolipids. The G+C content of the DNA was 64 mol%. On the basis of 16S rRNA gene sequence analysis, strain YSM-79T was determined to be a member of the family Halobacteriaceae, with the closest related genus being Halobacterium (94 % sequence identity). In addition, the rpoB′ gene sequence of strain YSM-79T had <88 % sequence similarity to those of other members of the family Halobacteriaceae. The results of phenotypic, chemotaxonomic and phylogenetic analysis suggested that strain YSM-79T should be placed in a new genus, Salarchaeum gen. nov., as a representative of Salarchaeum japonicum sp. nov. The type strain is YSM-79T ( = JCM 16327T  = CECT 7563T).

scholarly journals Halostagnicola kamekurae sp. nov., an extremely halophilic archaeon from solar salt

2010 ◽  
Vol 60 (12) ◽  
pp. 2828-2831 ◽  
Author(s):  
Shuhei Nagaoka ◽  
Hiroaki Minegishi ◽  
Akinobu Echigo ◽  
Ron Usami
Keyword(s):  
Lipid Analysis ◽  
Optimal Growth ◽  
The Philippines ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
Solar Salt ◽  
Physiological And Biochemical Characteristics ◽  
Gene Sequence Analysis ◽  
Extremely Halophilic Archaeon ◽  
Physiological And Biochemical

A novel extremely halophilic archaeon, strain 194-10T, was isolated from a solar salt sample imported into Japan from the Philippines. Strain 194-10T was pleomorphic, neutrophilic and mesophilic and required at least 10 % (w/v) NaCl but no MgSO4 . 7H2O for growth; it exhibited optimal growth at 15 % (w/v) NaCl and 60 mM MgSO4 . 7H2O. Strain 194-10T grew at 20–45 °C (optimum, 30 °C) and pH 6.0–9.0 (optimum, pH 6.5–7.0). The G+C content of its DNA was 59.8 mol%. 16S rRNA gene sequence analysis revealed closest proximity to Halostagnicola larsenii XH-48T (98.5 % similarity), the sole representative of the genus Halostagnicola. Polar lipid analysis revealed that strain 194-10T contained phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester (the latter derived from both C20C20 and C20C25 archaeol) and several unidentified glycolipids. The results of DNA–DNA hybridization (20.7 % relatedness between Hst. larsenii JCM 13463T and strain 194-10T) and physiological and biochemical characteristics allowed differentiation of strain 194-10T from Hst. larsenii XH-48T. Therefore, strain 194-10T represents a novel species of the genus Halostagnicola, for which the name Halostagnicola kamekurae sp. nov. is proposed, with the type strain 194-10T (=DSM 22427T =JCM 16110T =CECT 7536T).

Halopiger salifodinae sp. nov., an extremely halophilic archaeon isolated from a salt mine

2013 ◽  
Vol 63 (Pt_10) ◽  
pp. 3563-3567 ◽  
Author(s):  
Wei-Yan Zhang ◽  
Yuan Meng ◽  
Xu-Fen Zhu ◽  
Min Wu
Keyword(s):  
Type Species ◽  
Lipid Analysis ◽  
Rrna Gene ◽  
Salt Mine ◽  
Halophilic Archaeon ◽  
Content Type ◽  
Link Type ◽  
Extremely Halophilic Archaeon ◽  
Sulfated Glycolipid ◽  
Genotypic Characteristics

A novel extremely halophilic archaeon KCY07-B2T was isolated from a salt mine in Kuche county, Xinjiang province, China. Colonies were cream-pigmented and cells were pleomorphic rod-shaped. Strain KCY07-B2T was able to grow at 25–50 °C (optimum 37–45 °C) and pH 6.0–8.0 (optimum 7.0). The strain required at least 1.9 M NaCl for growth. MgCl2 was not required. Cells lysed in distilled water. Polar lipid analysis revealed the presence of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, derived from both C20C20 and C20C25 glycerol diethers, together with five glyolipids. The bis-sulfated glycolipid S2-DGD-1 was present. The DNA G+C content was 62.5 mol%. Analysis of the 16S rRNA gene sequence revealed that strain KCY07-B2T was closely related to Halopiger xanaduensis SH-6T and Halopiger aswanensis 56T (95.8 % and 95.5 % similarity, respectively). On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain KCY07-B2T is considered to represent a novel species of the genus Halopiger , for which the name Halopiger salifodinae sp. nov. is proposed. The type strain is KCY07-B2T ( = JCM 18547T = CGMCC 1.12284T).

scholarly journals Halalkalicoccus jeotgali sp. nov., a halophilic archaeon from shrimp jeotgal, a traditional Korean fermented seafood

2007 ◽  
Vol 57 (10) ◽  
pp. 2296-2298 ◽  
Author(s):  
Seong Woon Roh ◽  
Young-Do Nam ◽  
Ho-Won Chang ◽  
Youlboong Sung ◽  
Kyoung-Ho Kim ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Biochemical Tests ◽  
Extremely Halophilic Archaeon ◽  
Physiological And Biochemical

A novel, extremely halophilic archaeon B3T was isolated from shrimp-salted seafood. Its morphology, physiology, biochemical features and 16S rRNA gene sequence were characterized. Strain B3T is non-motile, Gram-variable, requires at least 10 % (w/v) NaCl for growth and grows in the ranges of 21–50 °C and pH 6.5–9.0. The DNA G+C content of strain B3T was 63.2 mol%. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain B3T belonged to the genus Halalkalicoccus and was phylogenetically closely related to the type strain Halalkalicoccus tibetensis (98.64 %). However, DNA–DNA hybridization experiments showed 7.0 % relatedness between strain B3T and a strain of a reference species of the genus Halalkalicoccus. Combined analysis of 16S rRNA gene sequences, DNA–DNA relatedness data, physiological and biochemical tests indicated that the genotypic and phenotypic characteristics differentiate strain B3T from other Halalkalicoccus species. On the basis of the evidence presented in this report, strain B3T represents a novel species of the genus Halalkalicoccus, for which the name Halalkalicoccus jeotgali. sp. nov. is proposed. The type strain is B3T (=KCTC 4019T=DSM 18796T=JCM 14584T=CECT 7217T).

scholarly journals Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae

2005 ◽  
Vol 55 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Olga I. Nedashkovskaya ◽  
Seung Bum Kim ◽  
Suk Kyun Han ◽  
Cindy Snauwaert ◽  
Marc Vancanneyt ◽  
...  
Keyword(s):  
Type Strain ◽  
New Genus ◽  
Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Respiratory Quinone ◽  
Peter The Great ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Major Respiratory Quinone

Three novel heterotrophic, Gram-negative, yellow-pigmented, aerobic, gliding, oxidase- and catalase-positive bacteria were isolated from algae collected in the Gulf of Peter the Great, Sea of Japan. 16S rRNA gene sequence analysis revealed that the strains studied represented members of the family Flavobacteriaceae and showed 93·5–93·8 % similarity with their closest relative, Psychroserpens burtonensis. The DNA G+C content of the strains was 34–37 mol%. The major respiratory quinone was MK-6. The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C15 : 1, iso-C16 : 0-3OH and iso-C17 : 0-3OH. On the basis of their phenotypic, chemotaxonomic, genotypic and phylogenetic characteristics, the newly described bacteria have been assigned to the new genus Winogradskyella gen. nov., as Winogradskyella thalassocola sp. nov. (type strain, KMM 3907T=KCTC 12221T=LMG 22492T=DSM 15363T), Winogradskyella epiphytica sp. nov. (type strain, KMM 3906T=KCTC 12220T=LMG 22491T=CCUG 47091T) and Winogradskyella eximia sp. nov. (type strain, KMM 3944T (=KCTC 12219T=LMG 22474T).

scholarly journals Salinadaptatus halalkaliphilus gen. nov., sp. nov., a haloalkaliphilic archaeon isolated from salt pond in Inner Mongolia Autonomous Region, China

2020 ◽  
Author(s):  
Qiong Xue ◽  
Zhenqiang Zuo ◽  
Heng Zhou ◽  
Jian Zhou ◽  
Shengjie Zhang ◽  
...  
Keyword(s):  
Inner Mongolia ◽  
Type Strain ◽  
Amino Acid Sequences ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Autonomous Region ◽  
Salt Pond ◽  
A Genome ◽  
The Family ◽  
Inner Mongolia Autonomous Region

A haloalkaliphilic strain XQ-INN 246T was isolated from the sediment of a salt pond in Inner Mongolia Autonomous Region, China. Cells of the strain were rods, motile and strictly aerobic. The strain was able to grow in the presence of 2.6–5.3 M NaCl (optimum concentration is 4.4 M) at 30–50 °C (optimum temperature is 42 °C) and pH 7.0–10.0 (optimum pH is 8.0–8.5). The whole genome sequencing of strain XQ-INN 246T revealed a genome size of 4.52 Mbp and a DNA G+C content of 62.06 mol%. Phylogenetic tree based on 16S rRNA gene sequences and concatenated amino acid sequences of 122 single-copy conserved proteins revealed a robust lineage of the strain XQ-INN 246T with members of related genera of the family Natrialbaceae . The strain possessed the polar lipids of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. No glycolipids were detected. Based on phylogenetic analysis, phenotypic characteristics, chemotaxonomic properties and genome relatedness, the isolate was proposed as the type strain of a novel species of a new genus within the family Natrialbaceae, for which the name Salinadaptatus halalkaliphilus gen. nov., sp. nov. is proposed. The type strain is XQ-INN 246T (=CGMCC 1.16692T=JCM 33751T).

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